Restriction map for pFN21AE0077
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvrII       BciVI       BclI        BfrI        BglII       
BlnI        BsaWI       BseAI       BsiWI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
CpoI        DraI        DrdI        Eam1105I    EcoRI       
EcoT22I     FbaI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MfeI        MluI        
MroI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tru9I       
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII        2260  CGCG                 CG^CG
AcsI          865  AAATTC               [GA]^AATT[CT]
AocI         1313  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2282  AGCGCT               AGC^GCT
ApoI          865  AAATTC               [GA]^AATT[CT]
AspI         3590  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2800  TGCGCA               TGC^GCA
BbeI         1667  GGCGCC               GGCGC^C
BbrPI        3103  CACGTG               CAC^GTG
BlpI         2704  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2704  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1728  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        2483  GAGACG               CGTCTC(1/5)
Bsp1407I     3368  TGTACA               T^GTACA
BsrBI         520  GAGCGG               CCGCTC(-3/-3)
BsrGI        3368  TGTACA               T^GTACA
BstUI        2260  CGCG                 CG^CG
Bsu36I       1313  CCTCAGG              CC^T[ACGT]AGG
CelII        2704  GCTCAGC              GC^T[ACGT]AGC
EagI          618  CGGCCG               C^GGCCG
EclXI         618  CGGCCG               C^GGCCG
Eco47III     2282  AGCGCT               AGC^GCT
Eco52I        618  CGGCCG               C^GGCCG
Eco81I       1313  CCTCAGG              CC^T[ACGT]AGG
EcoRV         628  GATATC               GAT^ATC
FseI         2808  GGCCGGCC             GGCCGG^CC
FspI         2800  TGCGCA               TGC^GCA
HgaI         2485  GACGC                GACGC(5/10)
KasI         1667  GGCGCC               G^GCGCC
MamI         1728  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         2260  CGCG                 CG^CG
NaeI         2809  GCCGGC               GCC^GGC
NarI         1667  GGCGCC               GG^CGCC
NgoMIV       2809  GCCGGC               G^CCGGC
PflFI        3590  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3103  CACGTG               CAC^GTG
PmlI         3103  CACGTG               CAC^GTG
PvuI            7  CGATCG               CGAT^CG
SfoI         1667  GGCGCC               GGC^GCC
SmaI         2789  CCCGGG               CCC^GGG
SspBI        3368  TGTACA               T^GTACA
Tth111I      3590  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2789  CCCGGG               C^CCGGG
XmaI         2789  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI         1667  GGCGCC               G[GA]^CG[CT]C
             2485  GACGCC              
Alw44I       2824  GTGCAC               G^TGCAC
             2919  GTGCAC              
ApaLI        2824  GTGCAC               G^TGCAC
             2919  GTGCAC              
BamHI         149  GGATCC               G^GATCC
              648  GGATCC              
BbsI         2754  GAAGAC               GAAGAC(2/6)
             1633  GTCTTC              
BbvCI        1647  CCTCAGC              CCTCAGC(-5/-2)
              930  GCTGAGG             
BcgI         1496  GCACCAGTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2921  GCACTTTCCTCG        
BpuAI        2754  GAAGAC               GAAGAC(2/6)
             1633  GTCTTC              
BsaAI         739  TACGTG               [CT]AC^GT[GA]
             3103  CACGTG              
BsaHI        1667  GGCGCC               G[GA]^CG[CT]C
             2485  GACGCC              
BsaI          911  GAGACC               GGTCTC(1/5)
              214  GGTCTC              
BsiEI           7  CGATCG               CG[GA][CT]^CG
              618  CGGCCG              
BspMI         611  ACCTGC               ACCTGC(4/8)
             3143  ACCTGC              
BsrFI        2316  ACCGGC               [GA]^CCGG[CT]
             2809  GCCGGC              
BssSI         829  CACGAG               CACGAG(-5/-1)
             3310  CTCGTG              
BstEII       1839  GGTCACC              G^GT[ACGT]ACC
             2223  GGTGACC             
BstPI        1839  GGTCACC              G^GT[ACGT]ACC
             2223  GGTGACC             
Cfr10I       2316  ACCGGC               [GA]^CCGG[CT]
             2809  GCCGGC              
DraIII        782  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1864  CACCTGGTG           
EcoNI        1737  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2205  CCTGTACGAGG         
EcoO65I      1839  GGTCACC              G^GT[ACGT]ACC
             2223  GGTGACC             
Hin1I        1667  GGCGCC               G[GA]^CG[CT]C
             2485  GACGCC              
NcoI          849  CCATGG               C^CATGG
             3303  CCATGG              
PpuMI         675  GGGTCCC              [GA]G^G[AT]CC[CT]
             1928  GGGACCT             
SacI         1900  GAGCTC               GAGCT^C
             2560  GAGCTC              
SexAI        1865  ACCTGGT              A^CC[AT]GGT
             3591  ACCAGGT             
SfiI          192  GGCCTTTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1086  GGCCCAGGAGGCC       
StuI          734  AGGCCT               AGG^CCT
             1317  AGGCCT              
Tsp509I       866  AATT                 ^AATT
              887  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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