(Circular) (Six-base) MAPSORT of: pFN21AE0077.seq Check: 174 from: 1 to: 8314 pFN21AE0077 8314 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6124 278 Size: 53 83 186 936 4588 2468 Fragments arranged by size: 4588 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5657 1937 Size: 3720 4594 Acc65I G'GTAC_C Cuts at: 5635 5635 Size: 8314 AclI AA'CG_TT Cuts at: 1563 6441 6814 1563 Size: 4878 373 3063 Fragments arranged by size: 4878 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 3198 3895 4276 6377 7761 8029 1632 Size: 482 1084 697 381 2101 1384 268 1917 Fragments arranged by size: 2101 1917 1384 1084 697 482 381 268 AfeI AGC'GCT Cuts at: 4289 4289 Size: 8314 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8117 AflIII A'CryG_T Cuts at: 1203 7213 8190 1203 Size: 6010 977 1327 Fragments arranged by size: 6010 1327 977 AgeI A'CCGG_T Cuts at: 7145 7145 Size: 8314 AhdI GACnn_n'nnGTC Cuts at: 7043 7043 Size: 8314 AleI CACnn'nnGTG Cuts at: 1166 4121 1166 Size: 2955 5359 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2981 3147 4001 4364 5196 1184 Size: 1797 166 854 363 832 4302 Fragments arranged by size: 4302 1797 854 832 363 166 AlwNI CAG_nnn'CTG Cuts at: 2131 2293 2347 2668 3379 3717 3742 3754 4876 Size: 162 54 321 711 338 25 12 1122 Cuts at: 4876 5537 5609 6057 7629 2131 Size: 661 72 448 1572 2816 Fragments arranged by size: 2816 1572 1122 711 661 448 338 321 162 72 54 25 12 ApaI G_GGCC'C Cuts at: 2209 4459 4795 5351 2209 Size: 2250 336 556 5172 Fragments arranged by size: 5172 2250 556 336 ApaLI G'TGCA_C Cuts at: 4829 4924 6371 7527 4829 Size: 95 1447 1156 5616 Fragments arranged by size: 5616 1447 1156 95 ApoI r'AATT_y Cuts at: 1493 2870 5623 5849 6001 8110 1493 Size: 1377 2753 226 152 2109 1697 Fragments arranged by size: 2753 2109 1697 1377 226 152 AseI AT'TA_AT Cuts at: 160 1032 6868 160 Size: 872 5836 1606 Fragments arranged by size: 5836 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 AvaI C'yCGr_G Cuts at: 1870 1943 2038 4158 4562 4794 5639 1870 Size: 73 95 2120 404 232 845 4545 Fragments arranged by size: 4545 2120 845 404 232 95 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5357 5390 5357 Size: 33 8281 BamHI G'GATC_C Cuts at: 2154 2653 5644 6011 2154 Size: 499 2991 367 4457 Fragments arranged by size: 4457 2991 499 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3455 3500 3672 3874 4153 Size: 325 217 627 1668 45 172 202 279 Cuts at: 4153 4163 4333 5516 5635 7090 618 Size: 10 170 1183 119 1455 1842 Fragments arranged by size: 1842 1668 1455 1183 627 325 279 217 202 172 170 119 45 10 BanII G_rGCy'C Cuts at: 1391 2209 2609 3333 3902 3909 4459 4569 4795 Size: 818 400 724 569 7 550 110 226 Cuts at: 4795 5351 5547 5633 1391 Size: 556 196 86 4072 Fragments arranged by size: 4072 818 724 569 556 550 400 226 196 110 86 7 BbeI G_GCGC'C Cuts at: 3676 3676 Size: 8314 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3631 4766 928 Size: 971 1732 1135 4476 Fragments arranged by size: 4476 1732 1135 971 BbvCI CC'TCA_GC Cuts at: 2936 3653 2936 Size: 717 7597 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3490 3524 4915 4949 6505 6539 1903 Size: 34 1553 34 1391 34 1556 34 3678 Fragments arranged by size: 3678 1556 1553 1391 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6206 7416 6206 Size: 1210 7104 BclI T'GATC_A Cuts at: 1548 1548 Size: 8314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2204 3098 4331 Size: 107 122 71 631 605 532 894 1233 Cuts at: 4331 4628 4820 6925 136 Size: 297 192 2105 1525 Fragments arranged by size: 2105 1525 1233 894 631 605 532 297 192 122 107 71 BglII A'GATC_T Cuts at: 8309 8309 Size: 8314 BlpI GC'TnA_GC Cuts at: 4710 5731 4710 Size: 1021 7293 Bme1580I G_kGCm'C Cuts at: 1790 2209 2797 3340 3503 3879 3997 4166 4459 Size: 419 588 543 163 376 118 169 293 Cuts at: 4459 4795 4833 4928 5351 6375 7531 1790 Size: 336 38 95 423 1024 1156 2573 Fragments arranged by size: 2573 1156 1024 588 543 423 419 376 336 293 169 163 118 95 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3256 3905 4411 Size: 416 53 83 399 24 1839 649 506 Cuts at: 4411 4723 5619 6045 7003 442 Size: 312 896 426 958 1753 Fragments arranged by size: 1839 1753 958 896 649 506 426 416 399 312 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8314 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2612 2612 Size: 8314 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2396 2690 3052 3449 3694 3939 4031 4506 Size: 1157 294 362 397 245 245 92 475 Cuts at: 4506 4739 6974 1239 Size: 233 2235 2579 Fragments arranged by size: 2579 2235 1157 475 397 362 294 245 245 233 92 Bpu10I CC'TnA_GC Cuts at: 2936 3653 3726 4059 4100 4184 4196 5426 2936 Size: 717 73 333 41 84 12 1230 5824 Fragments arranged by size: 5824 1230 717 333 84 73 41 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2717 4286 5189 6439 7304 7602 2717 Size: 1569 903 1250 865 298 3429 Fragments arranged by size: 3429 1569 1250 903 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 2910 6977 882 Size: 617 726 685 4067 2219 Fragments arranged by size: 4067 2219 726 685 617 BsaAI yAC'GTr Cuts at: 493 1204 2746 5110 6105 493 Size: 711 1542 2364 995 2702 Fragments arranged by size: 2702 2364 1542 995 711 BsaBI GATnn'nnATC Cuts at: 3737 8308 3737 Size: 4571 3743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4491 6121 6503 Size: 53 83 186 936 2140 818 1630 382 Cuts at: 6503 7138 275 Size: 635 1451 Fragments arranged by size: 2140 1630 1451 936 818 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6747 7145 7419 7566 7977 6747 Size: 398 274 147 411 7084 Fragments arranged by size: 7084 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2388 2418 4241 4271 1178 Size: 30 165 30 985 30 1823 30 5221 Fragments arranged by size: 5221 1823 985 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2687 3053 3130 3965 3968 4943 5294 Size: 1150 351 366 77 835 3 975 351 Cuts at: 5294 5434 1186 Size: 140 4066 Fragments arranged by size: 4066 1150 975 835 366 351 351 140 77 3 BseYI C'CCAG_C Cuts at: 2053 2084 2314 2677 2899 3120 3643 3754 4191 Size: 31 230 363 222 221 523 111 437 Cuts at: 4191 4823 4841 5215 7517 8013 2053 Size: 632 18 374 2302 496 2354 Fragments arranged by size: 2354 2302 632 523 496 437 374 363 230 222 221 111 31 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2747 3104 3598 4265 4334 4646 1163 Size: 573 1011 357 494 667 69 312 4831 Fragments arranged by size: 4831 1011 667 573 494 357 312 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 2626 5764 6525 7553 1540 Size: 275 186 14 611 3138 761 1028 2301 Fragments arranged by size: 3138 2301 1028 761 611 275 186 14 BsiHKAI G_wGCw'C Cuts at: 2181 2960 3699 3909 4080 4090 4569 4833 4928 Size: 779 739 210 171 10 479 264 95 Cuts at: 4928 4945 5175 5289 6375 6460 7531 2181 Size: 17 230 114 1086 85 1071 2964 Fragments arranged by size: 2964 1086 1071 779 739 479 264 230 210 171 114 95 85 17 10 BsmI GAATG_Cn' Cuts at: 5825 5918 5825 Size: 93 8221 BsmBI CGTCTCn'nnnn_ Cuts at: 4482 4482 Size: 8314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2209 2609 2797 2960 3333 3340 Size: 399 391 28 400 188 163 373 7 Cuts at: 3340 3503 3699 3879 3902 3909 3997 4080 4090 Size: 163 196 180 23 7 88 83 10 Cuts at: 4090 4166 4459 4569 4795 4833 4928 4945 5175 Size: 76 293 110 226 38 95 17 230 Cuts at: 5175 5289 5351 5547 5633 6375 6460 7531 1391 Size: 114 62 196 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 391 373 293 230 226 196 196 188 180 163 163 114 110 95 88 86 85 83 76 62 38 28 23 17 10 7 7 BspEI T'CCGG_A Cuts at: 7977 7977 Size: 8314 BspHI T'CATG_A Cuts at: 6203 7933 6203 Size: 1730 6584 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 5157 5669 844 Size: 1781 2532 512 3489 Fragments arranged by size: 3489 2532 1781 512 BsrBI CCG'CTC Cuts at: 1394 1823 2527 6201 1394 Size: 429 704 3674 3507 Fragments arranged by size: 3674 3507 704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3780 4444 5210 6809 6983 65 Size: 1183 2532 664 766 1599 174 1396 Fragments arranged by size: 2532 1599 1396 1183 766 664 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4814 6958 7145 1700 Size: 110 2511 493 2144 187 2869 Fragments arranged by size: 2869 2511 2144 493 187 110 BsrGI T'GTAC_A Cuts at: 96 5373 96 Size: 5277 3037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8314 BssSI C'ACGA_G Cuts at: 2834 5315 6374 7386 2834 Size: 2481 1059 1012 3762 Fragments arranged by size: 3762 2481 1059 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8314 BstBI TT'CG_AA Cuts at: 7118 7118 Size: 8314 BstEII G'GTnAC_C Cuts at: 1184 3844 4228 1184 Size: 2660 384 5270 Fragments arranged by size: 5270 2660 384 BstXI CCAn_nnnn'nTGG Cuts at: 3151 4072 5059 5215 5488 3151 Size: 921 987 156 273 5977 Fragments arranged by size: 5977 987 921 273 156 BstYI r'GATC_y Cuts at: 1972 2076 2154 2653 3126 3738 5391 5644 6011 Size: 104 78 499 473 612 1653 253 367 Cuts at: 6011 6396 6413 7865 7951 7963 8309 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1653 1452 612 499 473 385 367 346 253 104 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 3319 3319 Size: 8314 BtgI C'CryG_G Cuts at: 513 1065 2854 3276 4680 5058 5308 5487 513 Size: 552 1789 422 1404 378 250 179 3340 Fragments arranged by size: 3340 1789 1404 552 422 378 250 179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5009 487 Size: 683 56 3783 3792 Fragments arranged by size: 3792 3783 683 56 BtsI GCAGTG_nn' Cuts at: 2424 2779 3676 3792 4606 5830 6624 6644 2424 Size: 355 897 116 814 1224 794 20 4094 Fragments arranged by size: 4094 1224 897 814 794 355 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8279 DraI TTT'AAA Cuts at: 5618 5618 Size: 8314 DraIII CAC_nnn'GTG Cuts at: 2792 3874 2792 Size: 1082 7232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7321 809 Size: 507 6005 1802 Fragments arranged by size: 6005 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2623 2910 Size: 54 1425 50 275 18 399 394 287 Cuts at: 2910 3534 3597 4323 4816 4902 5490 5761 6650 Size: 624 63 726 493 86 588 271 889 Cuts at: 6650 8279 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 726 624 588 493 399 394 287 275 271 86 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2623 5761 1537 Size: 275 811 3138 4090 Fragments arranged by size: 4090 3138 811 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 3914 4095 4574 4865 6244 1353 Size: 788 1773 181 479 291 1379 3423 Fragments arranged by size: 3423 1773 1379 788 479 291 181 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2599 6901 7275 7421 387 Size: 1064 138 1010 4302 374 146 1280 Fragments arranged by size: 4302 1280 1064 1010 374 146 138 EcoICRI GAG'CTC Cuts at: 3907 4567 3907 Size: 660 7654 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2396 2690 3052 3198 3449 3694 Size: 393 482 282 294 362 146 251 245 Cuts at: 3694 3895 3939 4031 4276 4506 4739 6377 6974 Size: 201 44 92 245 230 233 1638 597 Cuts at: 6974 7761 8029 1239 Size: 787 268 1524 Fragments arranged by size: 1638 1524 787 597 482 393 362 294 282 268 251 245 245 233 230 201 146 92 44 EcoNI CCTnn'n_nnAGG Cuts at: 3746 4214 3746 Size: 468 7846 EcoO109I rG'GnC_Cy Cuts at: 2128 2205 2206 2681 2947 3100 3934 4455 4456 Size: 77 1 475 266 153 834 521 1 Cuts at: 4456 4791 2128 Size: 335 5651 Fragments arranged by size: 5651 834 521 475 335 266 153 77 1 1 EcoRI G'AATT_C Cuts at: 5623 6001 5623 Size: 378 7936 EcoRV GAT'ATC Cuts at: 2635 2635 Size: 8314 FseI GG_CCGG'CC Cuts at: 4818 4818 Size: 8314 FspI TGC'GCA Cuts at: 4807 6820 4807 Size: 2013 6301 HaeII r_GCGC'y Cuts at: 1678 3079 3676 3688 4191 4291 4891 5279 7461 Size: 1401 597 12 503 100 600 388 2182 Cuts at: 7461 1678 Size: 2531 Fragments arranged by size: 2531 2182 1401 600 597 503 388 100 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2139 2171 2364 2396 5383 Size: 32 135 32 567 32 193 32 2987 Cuts at: 5383 5415 6955 6987 7029 7061 1373 Size: 32 1540 32 42 32 2626 Fragments arranged by size: 2987 2626 1540 567 193 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5658 5904 669 Size: 988 281 3720 246 3079 Fragments arranged by size: 3720 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8314 HpaI GTT'AAC Cuts at: 5904 5904 Size: 8314 KasI G'GCGC_C Cuts at: 3672 3672 Size: 8314 KpnI G_GTAC'C Cuts at: 5639 5639 Size: 8314 MfeI C'AATT_G Cuts at: 5913 5913 Size: 8314 MluI A'CGCG_T Cuts at: 8190 8190 Size: 8314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7428 7612 1305 Size: 6123 184 2007 Fragments arranged by size: 6123 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2231 2912 5492 10 Size: 54 1425 343 399 681 2580 2832 Fragments arranged by size: 2832 2580 1425 681 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2769 4121 5057 5486 6272 6631 6790 Size: 648 1603 1352 936 429 786 359 159 Cuts at: 6790 518 Size: 2042 Fragments arranged by size: 2042 1603 1352 936 786 648 429 359 159 MspA1I CmG'CkG Cuts at: 1606 2665 2986 3073 3088 3999 4519 4845 5730 Size: 1059 321 87 15 911 520 326 885 Cuts at: 5730 6407 7555 7800 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1059 911 885 677 520 326 321 245 87 15 NaeI GCC'GGC Cuts at: 4816 4816 Size: 8314 NarI GG'CG_CC Cuts at: 3673 3673 Size: 8314 NcoI C'CATG_G Cuts at: 513 1065 2854 5308 513 Size: 552 1789 2454 3519 Fragments arranged by size: 3519 2454 1789 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8314 NgoMIV G'CCGG_C Cuts at: 4814 4814 Size: 8314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8314 NotI GC'GGCC_GC Cuts at: 5761 5761 Size: 8314 NspI r_CATG'y Cuts at: 1132 1229 3170 3434 3842 4058 4178 4806 5672 Size: 97 1941 264 408 216 120 628 866 Cuts at: 5672 7217 1132 Size: 1545 2229 Fragments arranged by size: 2229 1941 1545 866 628 408 264 216 120 97 PasI CC'CwG_GG Cuts at: 1348 3268 4234 4609 5134 1348 Size: 1920 966 375 525 4528 Fragments arranged by size: 4528 1920 966 525 375 PciI A'CATG_T Cuts at: 7213 7213 Size: 8314 PflMI CCAn_nnn'nTGG Cuts at: 1108 3754 3841 5609 1108 Size: 2646 87 1768 3813 Fragments arranged by size: 3813 2646 1768 87 PfoI T'CCnGG_A Cuts at: 2706 3465 2706 Size: 759 7555 PmeI GTTT'AAAC Cuts at: 5618 5618 Size: 8314 PmlI CAC'GTG Cuts at: 5110 5110 Size: 8314 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6383 6415 7896 7928 6383 Size: 32 1481 32 6769 Fragments arranged by size: 6769 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2681 3934 2681 Size: 1253 7061 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8314 PsiI TTA'TAA Cuts at: 5884 5884 Size: 8314 PspOMI G'GGCC_C Cuts at: 2205 4455 4791 5347 2205 Size: 2250 336 556 5172 Fragments arranged by size: 5172 2250 556 336 PstI C_TGCA'G Cuts at: 830 2955 3492 4768 5666 830 Size: 2125 537 1276 898 3478 Fragments arranged by size: 3478 2125 1276 898 537 PvuI CG_AT'CG Cuts at: 2001 2015 2001 Size: 14 8300 PvuII CAG'CTG Cuts at: 2665 2986 3088 4519 2665 Size: 321 102 1431 6460 Fragments arranged by size: 6460 1431 321 102 SacI G_AGCT'C Cuts at: 3909 4569 3909 Size: 660 7654 SalI G'TCGA_C Cuts at: 1936 5656 1936 Size: 3720 4594 SanDI GG'GwC_CC Cuts at: 2681 2681 Size: 8314 SapI GCTCTTCn'nnn_ Cuts at: 2141 3914 4095 4574 2141 Size: 1773 181 479 5881 Fragments arranged by size: 5881 1773 479 181 SbfI CC_TGCA'GG Cuts at: 5666 5666 Size: 8314 ScaI AGT'ACT Cuts at: 6562 8274 6562 Size: 1712 6602 SexAI A'CCwGG_T Cuts at: 3870 5596 3870 Size: 1726 6588 SfcI C'TryA_G Cuts at: 826 1045 2951 3488 3590 4370 4764 5079 5662 Size: 219 1906 537 102 780 394 315 583 Cuts at: 5662 6797 7478 7669 826 Size: 1135 681 191 1471 Fragments arranged by size: 1906 1471 1135 780 681 583 537 394 315 219 191 102 SfiI GGCCn_nnn'nGGCC Cuts at: 2204 3098 2204 Size: 894 7420 SfoI GGC'GCC Cuts at: 3674 3674 Size: 8314 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8314 SmaI CCC'GGG Cuts at: 1872 4796 5641 1872 Size: 2924 845 4545 Fragments arranged by size: 4545 2924 845 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 4301 5168 6418 7319 7581 Size: 197 926 753 1605 867 1250 901 262 Cuts at: 7581 820 Size: 1553 Fragments arranged by size: 1605 1553 1250 926 901 867 753 262 197 SnaBI TAC'GTA Cuts at: 493 6105 493 Size: 5612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8314 SphI G_CATG'C Cuts at: 1132 3170 3434 4178 4806 5672 1132 Size: 2038 264 744 628 866 3774 Fragments arranged by size: 3774 2038 866 744 628 264 SrfI GCCC'GGGC Cuts at: 4796 4796 Size: 8314 SspI AAT'ATT Cuts at: 5 52 6238 5 Size: 47 6186 2081 Fragments arranged by size: 6186 2081 47 StuI AGG'CCT Cuts at: 1517 2741 3324 1517 Size: 1224 583 6507 Fragments arranged by size: 6507 1224 583 StyI C'CwwG_G Cuts at: 513 1065 2304 2599 2854 2923 3325 4119 4167 Size: 552 1239 295 255 69 402 794 48 Cuts at: 4167 4716 5308 5549 5753 513 Size: 549 592 241 204 3074 Fragments arranged by size: 3074 1239 794 592 552 549 402 295 255 241 204 69 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4343 4582 5147 5207 5514 6341 6358 Size: 1255 2763 239 565 60 307 827 17 Cuts at: 6358 6511 6696 7141 325 Size: 153 185 445 1498 Fragments arranged by size: 2763 1498 1255 827 565 445 307 239 185 153 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5373 6560 8272 Size: 274 80 33 51 1443 3396 1187 1712 Cuts at: 8272 96 Size: 138 Fragments arranged by size: 3396 1712 1443 1187 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2061 2093 2388 2420 2624 2656 3390 3422 8274 Size: 32 295 32 204 32 734 32 4852 Cuts at: 8274 8306 2061 Size: 32 2069 Fragments arranged by size: 4852 2069 734 295 204 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5598 1316 Size: 4282 4032 XbaI T'CTAG_A Cuts at: 5650 5650 Size: 8314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2081 2165 2311 3529 3655 4531 4653 2081 Size: 84 146 1218 126 876 122 5742 Fragments arranged by size: 5742 1218 876 146 126 122 84 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8314 XmaI C'CCGG_G Cuts at: 1870 4794 5639 1870 Size: 2924 845 4545 Fragments arranged by size: 4545 2924 845 XmnI GAAnn'nnTTC Cuts at: 6443 6443 Size: 8314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6122 276 Size: 53 83 186 936 4588 2468 Fragments arranged by size: 4588 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BfrBI BsiWI BstZ17I FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SwaI