(Circular) (Six-base) MAPSORT of: pFN21AE0076.seq Check: 6349 from: 1 to: 9514 pFN21AE0076 9514 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4255 4286 6215 4255 Size: 31 1929 7554 Fragments arranged by size: 7554 1929 31 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3033 3849 7324 278 Size: 53 83 186 936 1497 816 3475 2468 Fragments arranged by size: 3475 2468 1497 936 816 186 83 53 AccI GT'mk_AC Cuts at: 1937 4197 6857 1937 Size: 2260 2660 4594 Fragments arranged by size: 4594 2660 2260 Acc65I G'GTAC_C Cuts at: 5212 6405 6699 6835 5212 Size: 1193 294 136 7891 Fragments arranged by size: 7891 1193 294 136 AclI AA'CG_TT Cuts at: 1563 7641 8014 1563 Size: 6078 373 3063 Fragments arranged by size: 6078 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 4060 4794 5101 7577 8961 9229 1632 Size: 1955 473 734 307 2476 1384 268 1917 Fragments arranged by size: 2476 1955 1917 1384 734 473 307 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9317 AflIII A'CryG_T Cuts at: 1203 8413 9390 1203 Size: 7210 977 1327 Fragments arranged by size: 7210 1327 977 AgeI A'CCGG_T Cuts at: 2302 8345 2302 Size: 6043 3471 AhdI GACnn_n'nnGTC Cuts at: 8243 8243 Size: 9514 AleI CACnn'nnGTG Cuts at: 1166 2427 1166 Size: 1261 8253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2928 1184 Size: 1744 7770 AlwNI CAG_nnn'CTG Cuts at: 2026 2416 2690 2830 2953 3064 3722 3784 4133 Size: 390 274 140 123 111 658 62 349 Cuts at: 4133 4301 5234 5252 6608 7257 8829 2026 Size: 168 933 18 1356 649 1572 2711 Fragments arranged by size: 2711 1572 1356 933 658 649 390 349 274 168 140 123 111 62 18 ApaI G_GGCC'C Cuts at: 4148 4336 5087 5137 5808 6746 6768 4148 Size: 188 751 50 671 938 22 6894 Fragments arranged by size: 6894 938 751 671 188 50 22 ApaLI G'TGCA_C Cuts at: 3941 4464 7571 8727 3941 Size: 523 3107 1156 4728 Fragments arranged by size: 4728 3107 1156 523 ApoI r'AATT_y Cuts at: 1493 6823 7049 7201 9310 1493 Size: 5330 226 152 2109 1697 Fragments arranged by size: 5330 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8068 160 Size: 872 7036 1606 Fragments arranged by size: 7036 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9514 AvaI C'yCGr_G Cuts at: 1870 1943 2795 2810 2876 3125 3230 4147 4398 Size: 73 852 15 66 249 105 917 251 Cuts at: 4398 5291 6122 6785 6839 1870 Size: 893 831 663 54 4545 Fragments arranged by size: 4545 917 893 852 831 663 251 249 105 73 66 54 15 AvrII C'CTAG_G Cuts at: 2615 2615 Size: 9514 BamHI G'GATC_C Cuts at: 6844 7211 6844 Size: 367 9147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2220 4167 4267 4347 4610 Size: 325 217 627 433 1947 100 80 263 Cuts at: 4610 4846 5212 5222 6074 6405 6699 6835 8290 Size: 236 366 10 852 331 294 136 1455 Cuts at: 8290 618 Size: 1842 Fragments arranged by size: 1947 1842 1455 852 627 433 366 331 325 294 263 236 217 136 100 80 10 BanII G_rGCy'C Cuts at: 1391 2454 3054 3090 3555 4148 4336 4488 5087 Size: 1063 600 36 465 593 188 152 599 Cuts at: 5087 5137 5421 5808 6276 6746 6768 6833 1391 Size: 50 284 387 468 470 22 65 4072 Fragments arranged by size: 4072 1063 600 599 593 470 468 465 387 284 188 152 65 50 36 22 BbeI G_GCGC'C Cuts at: 2224 2224 Size: 9514 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3483 3595 928 Size: 971 1584 112 6847 Fragments arranged by size: 6847 1584 971 112 BbvCI CC'TCA_GC Cuts at: 2823 4858 2823 Size: 2035 7479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 7705 7739 1903 Size: 34 50 34 5684 34 3678 Fragments arranged by size: 5684 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7406 8616 7406 Size: 1210 8304 BclI T'GATC_A Cuts at: 1548 1548 Size: 9514 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3067 3265 3725 Size: 107 122 71 631 605 1395 198 460 Cuts at: 3725 3740 3860 5335 5369 8125 136 Size: 15 120 1475 34 2756 1525 Fragments arranged by size: 2756 1525 1475 1395 631 605 460 198 122 120 107 71 34 15 BglII A'GATC_T Cuts at: 9509 9509 Size: 9514 BlpI GC'TnA_GC Cuts at: 3549 4487 5845 6418 6931 3549 Size: 938 1358 573 513 6132 Fragments arranged by size: 6132 1358 938 573 513 Bme1580I G_kGCm'C Cuts at: 1790 3072 3945 4148 4170 4272 4336 4350 4468 Size: 1282 873 203 22 102 64 14 118 Cuts at: 4468 4613 4639 4851 5087 5137 5225 5808 6395 Size: 145 26 212 236 50 88 583 587 Cuts at: 6395 6511 6746 6768 7575 8731 1790 Size: 116 235 22 807 1156 2573 Fragments arranged by size: 2573 1282 1156 873 807 587 583 236 235 212 203 145 118 116 102 88 64 50 26 22 22 14 BmgBI CAC'GTC Cuts at: 1318 5410 1318 Size: 4092 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2511 3374 3918 Size: 416 53 83 399 24 1094 863 544 Cuts at: 3918 4218 4266 4425 4688 4906 5368 5388 5512 Size: 300 48 159 263 218 462 20 124 Cuts at: 5512 5811 6230 7245 8203 442 Size: 299 419 1015 958 1753 Fragments arranged by size: 1753 1094 1015 958 863 544 462 419 416 399 300 299 263 218 159 124 83 53 48 24 20 BmtI G_CTAG'C Cuts at: 1056 6525 1056 Size: 5469 4045 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2767 5509 5521 2767 Size: 2742 12 6760 Fragments arranged by size: 6760 2742 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2909 2930 3068 4442 4785 5627 6275 6396 Size: 1670 21 138 1374 343 842 648 121 Cuts at: 6396 8174 1239 Size: 1778 2579 Fragments arranged by size: 2579 1778 1670 1374 842 648 343 138 121 21 Bpu10I CC'TnA_GC Cuts at: 2604 2610 2691 2823 3509 4858 6335 2604 Size: 6 81 132 686 1349 1477 5783 Fragments arranged by size: 5783 1477 1349 686 132 81 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3517 5557 6263 7639 8504 8802 3517 Size: 2040 706 1376 865 298 4229 Fragments arranged by size: 4229 2040 1376 865 706 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3346 3391 4823 5480 6012 8177 882 Size: 617 1847 45 1432 657 532 2165 2219 Fragments arranged by size: 2219 2165 1847 1432 657 617 532 45 BsaAI yAC'GTr Cuts at: 493 1204 7305 493 Size: 711 6101 2702 Fragments arranged by size: 6101 2702 711 BsaBI GATnn'nnATC Cuts at: 2381 9508 2381 Size: 7127 2387 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2221 2727 3030 3222 Size: 53 83 186 936 688 506 303 192 Cuts at: 3222 3816 3846 6177 7321 7703 8338 275 Size: 594 30 2331 1144 382 635 1451 Fragments arranged by size: 2331 1451 1144 936 688 635 594 506 382 303 192 186 83 53 30 BsaWI w'CCGG_w Cuts at: 2302 7947 8345 8619 8766 9177 2302 Size: 5645 398 274 147 411 2639 Fragments arranged by size: 5645 2639 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2159 2189 6482 6512 1178 Size: 30 165 30 756 30 4293 30 4180 Fragments arranged by size: 4293 4180 756 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2500 2561 3297 4540 5251 5254 5257 5260 Size: 1314 61 736 1243 711 3 3 3 Cuts at: 5260 5263 1186 Size: 3 5437 Fragments arranged by size: 5437 1314 1243 736 711 61 3 3 3 3 BseYI C'CCAG_C Cuts at: 2210 2334 2582 2628 2772 2838 3054 3457 3529 Size: 124 248 46 144 66 216 403 72 Cuts at: 3529 3670 3795 4133 4329 4647 4718 5039 6107 Size: 141 125 338 196 318 71 321 1068 Cuts at: 6107 6355 6380 8717 9213 2210 Size: 248 25 2337 496 2511 Fragments arranged by size: 2511 2337 1068 496 403 338 321 318 248 248 216 196 144 141 125 124 72 71 66 46 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 2390 2457 4010 1163 Size: 573 335 319 67 1553 6667 Fragments arranged by size: 6667 1553 573 335 319 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2307 3649 3711 6964 7725 8753 Size: 275 186 306 1342 62 3253 761 1028 Cuts at: 8753 1540 Size: 2301 Fragments arranged by size: 3253 2301 1342 1028 761 306 275 186 62 BsiHKAI G_wGCw'C Cuts at: 3110 3902 3945 4468 4488 5801 6121 7575 7660 Size: 792 43 523 20 1313 320 1454 85 Cuts at: 7660 8731 3110 Size: 1071 3893 Fragments arranged by size: 3893 1454 1313 1071 792 523 320 85 43 20 BsmI GAATG_Cn' Cuts at: 7025 7118 7025 Size: 93 9421 BsmBI CGTCTCn'nnnn_ Cuts at: 2304 4222 2304 Size: 1918 7596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2454 3054 3072 3090 3110 3555 3902 Size: 399 664 600 18 18 20 445 347 Cuts at: 3902 3945 4148 4170 4272 4336 4350 4468 4488 Size: 43 203 22 102 64 14 118 20 Cuts at: 4488 4613 4639 4851 5087 5137 5225 5421 5801 Size: 125 26 212 236 50 88 196 380 Cuts at: 5801 5808 6121 6276 6395 6511 6746 6768 6833 Size: 7 313 155 119 116 235 22 65 Cuts at: 6833 7575 7660 8731 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 600 445 399 380 347 313 236 235 212 203 196 155 125 119 118 116 102 88 85 65 64 50 43 26 22 22 20 20 18 18 14 7 BspEI T'CCGG_A Cuts at: 9177 9177 Size: 9514 BspHI T'CATG_A Cuts at: 2385 7403 9133 2385 Size: 5018 1730 2766 Fragments arranged by size: 5018 2766 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 2424 3672 4255 4286 4914 6215 6357 Size: 1520 60 1248 583 31 628 1301 142 Cuts at: 6357 6869 844 Size: 512 3489 Fragments arranged by size: 3489 1520 1301 1248 628 583 512 142 60 31 BsrBI CCG'CTC Cuts at: 1394 1823 2551 2578 2875 4872 5860 6753 7401 Size: 429 728 27 297 1997 988 893 648 Cuts at: 7401 1394 Size: 3507 Fragments arranged by size: 3507 1997 988 893 728 648 429 297 27 BsrDI GCAATG_nn' Cuts at: 65 1248 8009 8183 65 Size: 1183 6761 174 1396 Fragments arranged by size: 6761 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2302 3189 4514 5057 5530 6179 8158 Size: 110 492 887 1325 543 473 649 1979 Cuts at: 8158 8345 1700 Size: 187 2869 Fragments arranged by size: 2869 1979 1325 887 649 543 492 473 187 110 BsrGI T'GTAC_A Cuts at: 96 2431 96 Size: 2335 7179 BssHII G'CGCG_C Cuts at: 1925 4754 1925 Size: 2829 6685 BssSI C'ACGA_G Cuts at: 7574 8586 7574 Size: 1012 8502 BstAPI GCAn_nnn'nTGC Cuts at: 794 3988 4995 5252 5642 6608 794 Size: 3194 1007 257 390 966 3700 Fragments arranged by size: 3700 3194 1007 966 390 257 BstBI TT'CG_AA Cuts at: 8318 8318 Size: 9514 BstEII G'GTnAC_C Cuts at: 1184 3137 1184 Size: 1953 7561 BstXI CCAn_nnnn'nTGG Cuts at: 3268 3890 4420 4696 6259 3268 Size: 622 530 276 1563 6523 Fragments arranged by size: 6523 1563 622 530 276 BstYI r'GATC_y Cuts at: 1972 3177 3444 6844 7211 7596 7613 9065 9151 Size: 1205 267 3400 367 385 17 1452 86 Cuts at: 9151 9163 9509 1972 Size: 12 346 1977 Fragments arranged by size: 3400 1977 1452 1205 385 367 346 267 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2544 3132 4435 4471 5776 2544 Size: 588 1303 36 1305 6282 Fragments arranged by size: 6282 1305 1303 588 36 BtgI C'CryG_G Cuts at: 513 1065 2261 2658 2670 3261 3738 4122 4342 Size: 552 1196 397 12 591 477 384 220 Cuts at: 4342 4441 4863 5701 6165 6589 6768 513 Size: 99 422 838 464 424 179 3259 Fragments arranged by size: 3259 1196 838 591 552 477 464 424 422 397 384 220 179 99 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2790 487 Size: 683 56 1564 7211 Fragments arranged by size: 7211 1564 683 56 BtsI GCAGTG_nn' Cuts at: 3070 4707 5020 5978 6094 7030 7824 7844 3070 Size: 1637 313 958 116 936 794 20 4740 Fragments arranged by size: 4740 1637 958 936 794 313 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9514 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6154 6189 529 Size: 35 5590 35 3854 Fragments arranged by size: 5590 3854 35 35 DraI TTT'AAA Cuts at: 6818 6818 Size: 9514 DraIII CAC_nnn'GTG Cuts at: 3614 3926 6590 3614 Size: 312 2664 6538 Fragments arranged by size: 6538 2664 312 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5408 5479 8521 809 Size: 507 4092 71 3042 1802 Fragments arranged by size: 4092 3042 1802 507 71 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2523 2635 2667 Size: 54 1425 50 275 18 693 112 32 Cuts at: 2667 3081 3282 3438 3646 3708 3732 3741 3852 Size: 414 201 156 208 62 24 9 111 Cuts at: 3852 4033 4053 4119 4366 4659 5874 6082 6242 Size: 181 20 66 247 293 1215 208 160 Cuts at: 6242 6961 7850 9479 8 Size: 719 889 1629 43 Fragments arranged by size: 1629 1425 1215 889 719 693 414 293 275 247 208 208 201 181 160 156 112 111 66 62 54 50 43 32 24 20 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3646 3708 6961 1537 Size: 275 1834 62 3253 4090 Fragments arranged by size: 4090 3253 1834 275 62 EarI CTCTTCn'nnn_ Cuts at: 1353 4514 6228 7444 1353 Size: 3161 1714 1216 3423 Fragments arranged by size: 3423 3161 1714 1216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3208 3481 3601 3787 4371 4739 Size: 1064 138 1619 273 120 186 584 368 Cuts at: 4739 5725 5749 6788 8101 8475 8621 387 Size: 986 24 1039 1313 374 146 1280 Fragments arranged by size: 1619 1313 1280 1064 1039 986 584 374 368 273 186 146 138 120 24 EcoICRI GAG'CTC Cuts at: 4486 4486 Size: 9514 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2909 2930 3068 3587 4060 4442 4785 Size: 393 1277 21 138 519 473 382 343 Cuts at: 4785 4794 5101 5627 6275 6396 7577 8174 8961 Size: 9 307 526 648 121 1181 597 787 Cuts at: 8961 9229 1239 Size: 268 1524 Fragments arranged by size: 1524 1277 1181 787 648 597 526 519 473 393 382 343 307 268 138 121 21 9 EcoNI CCTnn'n_nnAGG Cuts at: 2237 2408 2637 5729 6188 2237 Size: 171 229 3092 459 5563 Fragments arranged by size: 5563 3092 459 229 171 EcoO109I rG'GnC_Cy Cuts at: 2023 2206 2392 2413 2791 2950 3674 3949 4144 Size: 183 186 21 378 159 724 275 195 Cuts at: 4144 4333 4654 5083 5084 5133 5134 5805 5838 Size: 189 321 429 1 49 1 671 33 Cuts at: 5838 5997 6038 6346 6742 6743 6764 2023 Size: 159 41 308 396 1 21 4773 Fragments arranged by size: 4773 724 671 429 396 378 321 308 275 195 189 186 183 159 159 49 41 33 21 21 1 1 1 EcoRI G'AATT_C Cuts at: 6823 7201 6823 Size: 378 9136 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3169 3880 5128 6772 3169 Size: 711 1248 1644 5911 Fragments arranged by size: 5911 1644 1248 711 FspI TGC'GCA Cuts at: 8020 8020 Size: 9514 HaeII r_GCGC'y Cuts at: 1678 2224 4501 5049 5058 8661 1678 Size: 546 2277 548 9 3603 2531 Fragments arranged by size: 3603 2531 2277 548 546 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2789 2821 3092 3124 3169 Size: 32 135 32 1217 32 271 32 45 Cuts at: 3169 3201 3920 3952 4535 4567 5348 5380 5573 Size: 32 719 32 583 32 781 32 193 Cuts at: 5573 5605 5624 5656 8155 8187 8229 8261 1373 Size: 32 19 32 2499 32 42 32 2626 Fragments arranged by size: 2626 2499 1217 781 719 583 271 193 135 45 42 32 32 32 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 5911 6858 7104 669 Size: 988 281 3973 947 246 3079 Fragments arranged by size: 3973 3079 988 947 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9514 HpaI GTT'AAC Cuts at: 7104 7104 Size: 9514 KasI G'GCGC_C Cuts at: 2220 2220 Size: 9514 KpnI G_GTAC'C Cuts at: 5216 6409 6703 6839 5216 Size: 1193 294 136 7891 Fragments arranged by size: 7891 1193 294 136 MfeI C'AATT_G Cuts at: 7113 7113 Size: 9514 MluI A'CGCG_T Cuts at: 9390 9390 Size: 9514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2287 2479 2553 5463 5466 5532 8628 8812 Size: 982 192 74 2910 3 66 3096 184 Cuts at: 8812 1305 Size: 2007 Fragments arranged by size: 3096 2910 2007 982 192 184 74 66 3 MscI TGG'CCA Cuts at: 10 64 1489 1832 2525 2637 3440 3743 3854 Size: 54 1425 343 693 112 803 303 111 Cuts at: 3854 4661 6084 10 Size: 807 1423 3440 Fragments arranged by size: 3440 1425 1423 807 803 693 343 303 112 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2427 3428 3743 7472 7831 7990 Size: 648 843 418 1001 315 3729 359 159 Cuts at: 7990 518 Size: 2042 Fragments arranged by size: 3729 2042 1001 843 648 418 359 315 159 MspA1I CmG'CkG Cuts at: 1606 2254 2292 2374 2776 2986 3562 3698 3731 Size: 648 38 82 402 210 576 136 33 Cuts at: 3731 3912 4133 4651 4713 5771 5873 6151 6317 Size: 181 221 518 62 1058 102 278 166 Cuts at: 6317 6355 6384 6422 6608 6930 7607 8755 9000 Size: 38 29 38 186 322 677 1148 245 Cuts at: 9000 1606 Size: 2120 Fragments arranged by size: 2120 1148 1058 677 648 576 518 402 322 278 245 221 210 186 181 166 136 102 82 62 38 38 38 33 29 NaeI GCC'GGC Cuts at: 3191 5059 6181 3191 Size: 1868 1122 6524 Fragments arranged by size: 6524 1868 1122 NarI GG'CG_CC Cuts at: 2221 2221 Size: 9514 NcoI C'CATG_G Cuts at: 513 1065 3738 4342 4863 6768 513 Size: 552 2673 604 521 1905 3259 Fragments arranged by size: 3259 2673 1905 604 552 521 NdeI CA'TA_TG Cuts at: 387 387 Size: 9514 NgoMIV G'CCGG_C Cuts at: 3189 5057 6179 3189 Size: 1868 1122 6524 Fragments arranged by size: 6524 1868 1122 NheI G'CTAG_C Cuts at: 1052 6521 1052 Size: 5469 4045 NotI GC'GGCC_GC Cuts at: 3646 6961 3646 Size: 3315 6199 NspI r_CATG'y Cuts at: 1132 1229 5974 6872 8417 1132 Size: 97 4745 898 1545 2229 Fragments arranged by size: 4745 2229 1545 898 97 PasI CC'CwG_GG Cuts at: 1348 2020 3064 1348 Size: 672 1044 7798 Fragments arranged by size: 7798 1044 672 PciI A'CATG_T Cuts at: 8413 8413 Size: 9514 PflMI CCAn_nnn'nTGG Cuts at: 1108 2026 2953 4286 6590 1108 Size: 918 927 1333 2304 4032 Fragments arranged by size: 4032 2304 1333 927 918 PfoI T'CCnGG_A Cuts at: 2070 2650 2829 2850 4235 4595 5430 5922 2070 Size: 580 179 21 1385 360 835 492 5662 Fragments arranged by size: 5662 1385 835 580 492 360 179 21 PmeI GTTT'AAAC Cuts at: 6818 6818 Size: 9514 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7583 7615 9096 9128 7583 Size: 32 1481 32 7969 Fragments arranged by size: 7969 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2392 2413 3674 6038 6346 2206 Size: 186 21 1261 2364 308 5374 Fragments arranged by size: 5374 2364 1261 308 186 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9514 PsiI TTA'TAA Cuts at: 7084 7084 Size: 9514 PspOMI G'GGCC_C Cuts at: 4144 4332 5083 5133 5804 6742 6764 4144 Size: 188 751 50 671 938 22 6894 Fragments arranged by size: 6894 938 751 671 188 50 22 PspXI vC'TCGA_Gb Cuts at: 2795 2795 Size: 9514 PstI C_TGCA'G Cuts at: 830 2421 5000 5248 6189 6322 6604 6684 6866 Size: 1591 2579 248 941 133 282 80 182 Cuts at: 6866 830 Size: 3478 Fragments arranged by size: 3478 2579 1591 941 282 248 182 133 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9514 PvuII CAG'CTG Cuts at: 2374 2776 2986 3698 6317 6384 6422 2374 Size: 402 210 712 2619 67 38 5466 Fragments arranged by size: 5466 2619 712 402 210 67 38 SacI G_AGCT'C Cuts at: 4488 4488 Size: 9514 SalI G'TCGA_C Cuts at: 1936 6856 1936 Size: 4920 4594 SanDI GG'GwC_CC Cuts at: 2206 3674 2206 Size: 1468 8046 SapI GCTCTTCn'nnn_ Cuts at: 6228 6228 Size: 9514 SbfI CC_TGCA'GG Cuts at: 6684 6866 6684 Size: 182 9332 ScaI AGT'ACT Cuts at: 2084 3839 7762 9474 2084 Size: 1755 3923 1712 2124 Fragments arranged by size: 3923 2124 1755 1712 SfcI C'TryA_G Cuts at: 826 1045 2417 2492 4453 4996 5244 5953 6185 Size: 219 1372 75 1961 543 248 709 232 Cuts at: 6185 6318 6600 6680 6694 6862 7997 8678 8869 Size: 133 282 80 14 168 1135 681 191 Cuts at: 8869 826 Size: 1471 Fragments arranged by size: 1961 1471 1372 1135 709 681 543 282 248 232 219 191 168 133 80 75 14 SfiI GGCCn_nnn'nGGCC Cuts at: 3740 3860 5369 3740 Size: 120 1509 7885 Fragments arranged by size: 7885 1509 120 SfoI GGC'GCC Cuts at: 2222 2222 Size: 9514 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9514 SgrAI Cr'CCGG_yG Cuts at: 1700 3189 1700 Size: 1489 8025 SmaI CCC'GGG Cuts at: 1872 3127 4149 4400 6841 1872 Size: 1255 1022 251 2441 4545 Fragments arranged by size: 4545 2441 1255 1022 251 SmlI C'TyrA_G Cuts at: 820 1017 1943 2795 3496 5572 6278 7618 8519 Size: 197 926 852 701 2076 706 1340 901 Cuts at: 8519 8781 820 Size: 262 1553 Fragments arranged by size: 2076 1553 1340 926 901 852 706 701 262 197 SnaBI TAC'GTA Cuts at: 493 7305 493 Size: 6812 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9514 SphI G_CATG'C Cuts at: 1132 6872 1132 Size: 5740 3774 SrfI GCCC'GGGC Cuts at: 4149 4400 4149 Size: 251 9263 SspI AAT'ATT Cuts at: 5 52 7438 5 Size: 47 7386 2081 Fragments arranged by size: 7386 2081 47 StuI AGG'CCT Cuts at: 1517 2156 5108 5352 5372 5570 5735 1517 Size: 639 2952 244 20 198 165 5296 Fragments arranged by size: 5296 2952 639 244 198 165 20 StyI C'CwwG_G Cuts at: 513 1065 2238 2615 2638 2973 3687 3738 4342 Size: 552 1173 377 23 335 714 51 604 Cuts at: 4342 4683 4863 5217 6085 6540 6768 6953 513 Size: 341 180 354 868 455 228 185 3074 Fragments arranged by size: 3074 1173 868 714 604 552 455 377 354 341 335 228 185 180 51 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2180 2530 3937 3974 4185 4365 4406 Size: 1255 600 350 1407 37 211 180 41 Cuts at: 4406 4496 4642 4898 5053 5531 6104 6174 6178 Size: 90 146 256 155 478 573 70 4 Cuts at: 6178 6725 7541 7558 7711 7896 8341 325 Size: 547 816 17 153 185 445 1498 Fragments arranged by size: 1498 1407 1255 816 600 573 547 478 445 350 256 211 185 180 155 153 146 90 70 41 37 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 2431 2496 Size: 274 80 33 51 1443 105 349 65 Cuts at: 2496 3837 7760 9472 96 Size: 1341 3923 1712 138 Fragments arranged by size: 3923 1712 1443 1341 349 274 138 105 80 65 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 3910 3942 5653 5685 6359 6391 6482 Size: 32 1719 32 1711 32 674 32 91 Cuts at: 6482 6514 9474 9506 2159 Size: 32 2960 32 2167 Fragments arranged by size: 2960 2167 1719 1711 674 91 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6646 1316 Size: 5330 4184 XbaI T'CTAG_A Cuts at: 6850 6850 Size: 9514 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3268 5428 6725 3268 Size: 2160 1297 6057 Fragments arranged by size: 6057 2160 1297 XhoI C'TCGA_G Cuts at: 1943 2795 1943 Size: 852 8662 XmaI C'CCGG_G Cuts at: 1870 3125 4147 4398 6839 1870 Size: 1255 1022 251 2441 4545 Fragments arranged by size: 4545 2441 1255 1022 251 XmnI GAAnn'nnTTC Cuts at: 7643 7643 Size: 9514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3031 3847 7322 276 Size: 53 83 186 936 1497 816 3475 2468 Fragments arranged by size: 3475 2468 1497 936 816 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SexAI SwaI