Restriction map for
pFN21AE0070
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI BamHI
BbrPI BcgI BciVI BfrI BglII
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrDI BsrGI Bst1107I BstYI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI HindIII
KpnI MamI MfeI MflI MluI
MroI MslI MunI NcoI NdeI
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
ScaI SexAI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 951 GTAC GT^AC
AocI 306 CCTGAGG CC^T[ACGT]AGG
AseI 1656 ATTAAT AT^TAAT
AsnI 1656 ATTAAT AT^TAAT
AvrII 1317 CCTAGG C^CTAGG
BalI 1638 TGGCCA TGG^CCA
BbsI 82 GTCTTC GAAGAC(2/6)
BbvCI 3 GCTGAGG CCTCAGC(-5/-2)
BlnI 1317 CCTAGG C^CTAGG
BpuAI 82 GTCTTC GAAGAC(2/6)
BsaI 1198 GAGACC GGTCTC(1/5)
BseRI 1458 CTCCTC GAGGAG(10/8)
BsgI 1036 CTGCAC GTGCAG(16/14)
BsiEI 714 CGACCG CG[GA][CT]^CG
BspHI 236 TCATGA T^CATGA
BspLU11I 419 ACATGT A^CATGT
BsrBI 1381 CCGCTC CCGCTC(-3/-3)
BssSI 1324 CTCGTG CACGAG(-5/-1)
BstAPI 159 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1649 TTCGAA TT^CGAA
BstEII 1527 GGTGACC G^GT[ACGT]ACC
BstPI 1527 GGTGACC G^GT[ACGT]ACC
BstXI 1005 CCAGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 306 CCTGAGG CC^T[ACGT]AGG
EaeI 1638 TGGCCA [CT]^GGCC[GA]
Eco81I 306 CCTGAGG CC^T[ACGT]AGG
EcoNI 552 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1527 GGTGACC G^GT[ACGT]ACC
HpaI 436 GTTAAC GTT^AAC
MluNI 1638 TGGCCA TGG^CCA
MscI 1638 TGGCCA TGG^CCA
NaeI 1372 GCCGGC GCC^GGC
NgoMIV 1372 GCCGGC G^CCGGC
NspI 419 ACATGT [GA]CATG^[CT]
NspV 1649 TTCGAA TT^CGAA
PaeR7I 586 CTCGAG C^TCGAG
PpuMI 1244 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1239 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1656 ATTAAT AT^TAAT
RcaI 236 TCATGA T^CATGA
RsaI 951 GTAC GT^AC
SfiI 1420 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1649 TTCGAA TT^CGAA
SmlI 586 CTCGAG C^T[CT][GA]AG
XhoI 586 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 419 ACATGT A^C[GA][CT]GT
1214 ACGTGT
AvaII 387 GGACC G^G[AT]CC
1245 GGTCC
AviII 905 TGCGCA TGC^GCA
1351 TGCGCA
BbeI 652 GGCGCC GGCGC^C
997 GGCGCC
BclI 1601 TGATCA T^GATCA
1610 TGATCA
BfaI 136 CTAG C^TAG
1318 CTAG
BglI 1322 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1421 GCCCCCGGGGC
BlpI 580 GCTAAGC GC^T[ACGT]AGC
978 GCTGAGC
Bpu1102I 580 GCTAAGC GC^T[ACGT]AGC
978 GCTGAGC
BspMI 440 ACCTGC ACCTGC(4/8)
551 ACCTGC
BsrI 432 ACTGG ACTGG(1/-1)
488 CCAGT
BstF5I 162 CATCC GGATG(2/0)
687 CATCC
CelII 580 GCTAAGC GC^T[ACGT]AGC
978 GCTGAGC
FbaI 1601 TGATCA T^GATCA
1610 TGATCA
FokI 162 CATCC GGATG(9/13)
687 CATCC
FspI 905 TGCGCA TGC^GCA
1351 TGCGCA
HincII 436 GTTAAC GT[CT]^[GA]AC
810 GTTGAC
HindII 436 GTTAAC GT[CT]^[GA]AC
810 GTTGAC
KasI 652 GGCGCC G^GCGCC
997 GGCGCC
KspI 183 CCGCGG CCGC^GG
973 CCGCGG
MaeI 136 CTAG C^TAG
1318 CTAG
MaeII 116 ACGT A^CGT
1214 ACGT
NarI 652 GGCGCC GG^CGCC
997 GGCGCC
PvuII 901 CAGCTG CAG^CTG
1192 CAGCTG
SacII 183 CCGCGG CCGC^GG
973 CCGCGG
SbfI 441 CCTGCAGG CCTGCA^GG
873 CCTGCAGG
SfaNI 125 GCATC GCATC(5/9)
333 GCATC
SfoI 652 GGCGCC GGC^GCC
997 GGCGCC
Sse8387I 441 CCTGCAGG CCTGCA^GG
873 CCTGCAGG
StuI 138 AGGCCT AGG^CCT
1320 AGGCCT
Tsp509I 1635 AATT ^AATT
1655 AATT
TspRI 100 CAGTG CA[GC]TG[ACGT][ACGT]^
282 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AspHI AvaI BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
SacI SapI Sau3AI Sau96I ScrFI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I TthHB8I XmaCI
XmaI