(Circular) (Six-base) MAPSORT of: pFN21AE0066.seq Check: 3406 from: 1 to: 7384 pFN21AE0066 7384 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5194 278 Size: 53 83 186 936 3658 2468 Fragments arranged by size: 3658 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2640 2682 2697 4727 1937 Size: 703 42 15 2030 4594 Fragments arranged by size: 4594 2030 703 42 15 Acc65I G'GTAC_C Cuts at: 2813 4705 2813 Size: 1892 5492 AclI AA'CG_TT Cuts at: 1563 5511 5884 1563 Size: 3948 373 3063 Fragments arranged by size: 3948 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2311 2708 3095 3788 4392 4632 5447 6831 Size: 679 397 387 693 604 240 815 1384 Cuts at: 6831 7099 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 815 693 679 604 397 387 268 240 AfeI AGC'GCT Cuts at: 3821 3821 Size: 7384 AflII C'TTAA_G Cuts at: 820 1017 3131 820 Size: 197 2114 5073 Fragments arranged by size: 5073 2114 197 AflIII A'CryG_T Cuts at: 1203 2532 4444 4446 6283 7260 1203 Size: 1329 1912 2 1837 977 1327 Fragments arranged by size: 1912 1837 1329 1327 977 2 AgeI A'CCGG_T Cuts at: 6215 6215 Size: 7384 AhdI GACnn_n'nnGTC Cuts at: 6113 6113 Size: 7384 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7384 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7384 AlwNI CAG_nnn'CTG Cuts at: 2495 4007 4108 4225 5127 6699 2495 Size: 1512 101 117 902 1572 3180 Fragments arranged by size: 3180 1572 1512 902 117 101 ApaI G_GGCC'C Cuts at: 2174 2174 Size: 7384 ApaLI G'TGCA_C Cuts at: 5441 6597 5441 Size: 1156 6228 ApoI r'AATT_y Cuts at: 1493 4693 4919 5071 7180 1493 Size: 3200 226 152 2109 1697 Fragments arranged by size: 3200 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5938 160 Size: 872 4906 1606 Fragments arranged by size: 4906 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7384 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2087 2160 2222 2249 3838 4709 Size: 73 76 68 73 62 27 1589 871 Cuts at: 4709 1870 Size: 4545 Fragments arranged by size: 4545 1589 871 76 73 73 68 62 27 BamHI G'GATC_C Cuts at: 4714 5081 4714 Size: 367 7017 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2183 2209 2813 4705 Size: 325 217 627 369 27 26 604 1892 Cuts at: 4705 6160 618 Size: 1455 1842 Fragments arranged by size: 1892 1842 1455 627 604 369 325 217 27 26 BanII G_rGCy'C Cuts at: 1391 2174 2322 3294 4581 4703 1391 Size: 783 148 972 1287 122 4072 Fragments arranged by size: 4072 1287 972 783 148 122 BbeI G_GCGC'C Cuts at: 2160 2187 2213 2160 Size: 27 26 7331 Fragments arranged by size: 7331 27 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2430 3309 4380 928 Size: 971 531 879 1071 3932 Fragments arranged by size: 3932 1071 971 879 531 BbvCI CC'TCA_GC Cuts at: 3779 4238 3779 Size: 459 6925 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5575 5609 1903 Size: 34 3638 34 3678 Fragments arranged by size: 3678 3638 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2810 3078 5276 6486 2810 Size: 268 2198 1210 3708 Fragments arranged by size: 3708 2198 1210 268 BclI T'GATC_A Cuts at: 1548 4477 1548 Size: 2929 4455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 2192 4011 Size: 107 122 71 631 605 454 66 1819 Cuts at: 4011 5995 136 Size: 1984 1525 Fragments arranged by size: 1984 1819 1525 631 605 454 122 107 71 66 BglII A'GATC_T Cuts at: 7379 7379 Size: 7384 BlpI GC'TnA_GC Cuts at: 2079 2234 2321 4801 2079 Size: 155 87 2480 4662 Fragments arranged by size: 4662 2480 155 87 Bme1580I G_kGCm'C Cuts at: 1790 2174 3624 5445 6601 1790 Size: 384 1450 1821 1156 2573 Fragments arranged by size: 2573 1821 1450 1156 384 BmgBI CAC'GTC Cuts at: 1318 2644 1318 Size: 1326 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4289 4630 5115 Size: 416 53 83 399 24 2872 341 485 Cuts at: 5115 6073 442 Size: 958 1753 Fragments arranged by size: 2872 1753 958 485 416 399 341 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7384 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3449 3894 3984 4117 6044 1239 Size: 2210 445 90 133 1927 2579 Fragments arranged by size: 2579 2210 1927 445 133 90 Bpu10I CC'TnA_GC Cuts at: 2615 3102 3779 4238 2615 Size: 487 677 459 5761 Fragments arranged by size: 5761 677 487 459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3442 3915 5509 6374 6672 2369 Size: 1073 473 1594 865 298 3081 Fragments arranged by size: 3081 1594 1073 865 473 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4002 6047 882 Size: 617 2503 2045 2219 Fragments arranged by size: 2503 2219 2045 617 BsaAI yAC'GTr Cuts at: 493 1204 4447 5175 493 Size: 711 3243 728 2702 Fragments arranged by size: 3243 2702 728 711 BsaBI GATnn'nnATC Cuts at: 7378 7378 Size: 7384 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2157 2184 2210 Size: 53 83 186 936 476 148 27 26 Cuts at: 2210 5191 5573 6208 275 Size: 2981 382 635 1451 Fragments arranged by size: 2981 1451 936 635 476 382 186 148 83 53 27 26 BsaWI w'CCGG_w Cuts at: 5817 6215 6489 6636 7047 5817 Size: 398 274 147 411 6154 Fragments arranged by size: 6154 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7159 Fragments arranged by size: 7159 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 2444 4120 4534 1186 Size: 1144 114 1676 414 4036 Fragments arranged by size: 4036 1676 1144 414 114 BseYI C'CCAG_C Cuts at: 2106 2195 2305 2331 4080 4181 6587 7083 2106 Size: 89 110 26 1749 101 2406 496 2407 Fragments arranged by size: 2407 2406 1749 496 110 101 89 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4094 1163 Size: 573 2358 4453 Fragments arranged by size: 4453 2358 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 4834 5595 6623 1540 Size: 275 186 120 2713 761 1028 2301 Fragments arranged by size: 2713 2301 1028 761 275 186 120 BsiHKAI G_wGCw'C Cuts at: 2105 3294 3885 5445 5530 6601 2105 Size: 1189 591 1560 85 1071 2888 Fragments arranged by size: 2888 1560 1189 1071 591 85 BsmI GAATG_Cn' Cuts at: 4895 4988 4895 Size: 93 7291 BsmBI CGTCTCn'nnnn_ Cuts at: 3729 3729 Size: 7384 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2174 2322 3294 3624 3885 4581 Size: 399 315 69 148 972 330 261 696 Cuts at: 4581 4703 5445 5530 6601 1391 Size: 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 972 742 696 399 330 315 261 148 122 85 69 BspEI T'CCGG_A Cuts at: 7047 7047 Size: 7384 BspHI T'CATG_A Cuts at: 5273 7003 5273 Size: 1730 5654 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 4739 844 Size: 1631 2264 3489 Fragments arranged by size: 3489 2264 1631 BsrBI CCG'CTC Cuts at: 1394 1823 2154 5271 1394 Size: 429 331 3117 3507 Fragments arranged by size: 3507 3117 429 331 BsrDI GCAATG_nn' Cuts at: 65 1248 3527 4359 5879 6053 65 Size: 1183 2279 832 1520 174 1396 Fragments arranged by size: 2279 1520 1396 1183 832 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 4094 6028 6215 1700 Size: 110 376 1908 1934 187 2869 Fragments arranged by size: 2869 1934 1908 376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7384 BssHII G'CGCG_C Cuts at: 1925 2406 2455 1925 Size: 481 49 6854 Fragments arranged by size: 6854 481 49 BssSI C'ACGA_G Cuts at: 5444 6456 5444 Size: 1012 6372 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7384 BstBI TT'CG_AA Cuts at: 6188 6188 Size: 7384 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7384 BstXI CCAn_nnnn'nTGG Cuts at: 2810 4141 2810 Size: 1331 6053 BstYI r'GATC_y Cuts at: 1972 3106 4287 4714 5081 5466 5483 6935 7021 Size: 1134 1181 427 367 385 17 1452 86 Cuts at: 7021 7033 7379 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1181 1134 427 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2683 2683 Size: 7384 Bsu36I CC'TnA_GG Cuts at: 2660 2885 4089 4101 4671 2660 Size: 225 1204 12 570 5373 Fragments arranged by size: 5373 1204 570 225 12 BtgI C'CryG_G Cuts at: 513 1065 2377 513 Size: 552 1312 5520 Fragments arranged by size: 5520 1312 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2162 4171 4195 4691 487 Size: 683 56 936 2009 24 496 3180 Fragments arranged by size: 3180 2009 936 683 496 56 24 BtsI GCAGTG_nn' Cuts at: 2137 4057 4900 5694 5714 2137 Size: 1920 843 794 20 3807 Fragments arranged by size: 3807 1920 843 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7384 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7349 DraI TTT'AAA Cuts at: 4688 4688 Size: 7384 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 4391 6391 809 Size: 507 698 2377 2000 1802 Fragments arranged by size: 2377 2000 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 4831 5720 Size: 54 1425 50 275 18 288 2713 889 Cuts at: 5720 7349 8 Size: 1629 43 Fragments arranged by size: 2713 1629 1425 889 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2118 4831 1537 Size: 275 306 2713 4090 Fragments arranged by size: 4090 2713 306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5314 1353 Size: 3961 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 2056 5971 6345 6491 387 Size: 1064 138 450 17 3915 374 146 1280 Fragments arranged by size: 3915 1280 1064 450 374 146 138 17 EcoICRI GAG'CTC Cuts at: 3292 3292 Size: 7384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2311 2708 3095 3449 3788 3894 3984 Size: 393 679 397 387 354 339 106 90 Cuts at: 3984 4117 4392 4632 5447 6044 6831 7099 1239 Size: 133 275 240 815 597 787 268 1524 Fragments arranged by size: 1524 815 787 679 597 397 393 387 354 339 275 268 240 133 106 90 EcoNI CCTnn'n_nnAGG Cuts at: 3467 3467 Size: 7384 EcoO109I rG'GnC_Cy Cuts at: 2170 2389 3715 3772 4004 2170 Size: 219 1326 57 232 5550 Fragments arranged by size: 5550 1326 232 219 57 EcoRI G'AATT_C Cuts at: 4693 5071 4693 Size: 378 7006 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3337 3436 4598 3337 Size: 99 1162 6123 Fragments arranged by size: 6123 1162 99 FseI GG_CCGG'CC Cuts at: 4098 4098 Size: 7384 FspI TGC'GCA Cuts at: 5890 5890 Size: 7384 HaeII r_GCGC'y Cuts at: 1678 2068 2160 2187 2213 2417 3370 3712 3823 Size: 390 92 27 26 204 953 342 111 Cuts at: 3823 6531 1678 Size: 2708 2531 Fragments arranged by size: 2708 2531 953 390 342 204 111 92 27 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2969 3001 4654 4686 6025 Size: 32 135 32 1397 32 1653 32 1339 Cuts at: 6025 6057 6099 6131 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1653 1397 1339 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2641 4728 4974 669 Size: 988 281 703 2087 246 3079 Fragments arranged by size: 3079 2087 988 703 281 246 HindIII A'AGCT_T Cuts at: 748 4325 4483 748 Size: 3577 158 3649 Fragments arranged by size: 3649 3577 158 HpaI GTT'AAC Cuts at: 4974 4974 Size: 7384 KasI G'GCGC_C Cuts at: 2156 2183 2209 2156 Size: 27 26 7331 Fragments arranged by size: 7331 27 26 KpnI G_GTAC'C Cuts at: 2817 4709 2817 Size: 1892 5492 MfeI C'AATT_G Cuts at: 4983 4983 Size: 7384 MluI A'CGCG_T Cuts at: 7260 7260 Size: 7384 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2842 6498 6682 1305 Size: 1537 3656 184 2007 Fragments arranged by size: 3656 2007 1537 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5562 Fragments arranged by size: 5562 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 3443 3809 3860 5342 5701 5860 Size: 648 1656 621 366 51 1482 359 159 Cuts at: 5860 518 Size: 2042 Fragments arranged by size: 2042 1656 1482 648 621 366 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2266 2331 2379 3787 4246 4294 4800 5477 Size: 660 65 48 1408 459 48 506 677 Cuts at: 5477 6625 6870 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1408 1148 677 660 506 459 245 65 48 48 NaeI GCC'GGC Cuts at: 2188 4096 2188 Size: 1908 5476 NarI GG'CG_CC Cuts at: 2157 2184 2210 2157 Size: 27 26 7331 Fragments arranged by size: 7331 27 26 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6832 NdeI CA'TA_TG Cuts at: 387 3987 387 Size: 3600 3784 NgoMIV G'CCGG_C Cuts at: 2186 4094 2186 Size: 1908 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7384 NotI GC'GGCC_GC Cuts at: 4831 4831 Size: 7384 NspI r_CATG'y Cuts at: 1132 1229 2536 4346 4402 4742 6287 1132 Size: 97 1307 1810 56 340 1545 2229 Fragments arranged by size: 2229 1810 1545 1307 340 97 56 PasI CC'CwG_GG Cuts at: 1348 3757 4264 1348 Size: 2409 507 4468 Fragments arranged by size: 4468 2409 507 PciI A'CATG_T Cuts at: 2532 6283 2532 Size: 3751 3633 PflMI CCAn_nnn'nTGG Cuts at: 1108 4007 4043 4629 1108 Size: 2899 36 586 3863 Fragments arranged by size: 3863 2899 586 36 PfoI T'CCnGG_A Cuts at: 2829 3268 2829 Size: 439 6945 PmeI GTTT'AAAC Cuts at: 4688 4688 Size: 7384 PmlI CAC'GTG Cuts at: 4447 4447 Size: 7384 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5453 5485 6966 6998 5453 Size: 32 1481 32 5839 Fragments arranged by size: 5839 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3715 3772 3715 Size: 57 7327 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7384 PsiI TTA'TAA Cuts at: 4954 4954 Size: 7384 PspOMI G'GGCC_C Cuts at: 2170 2170 Size: 7384 PstI C_TGCA'G Cuts at: 830 4352 4736 830 Size: 3522 384 3478 Fragments arranged by size: 3522 3478 384 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7384 PvuII CAG'CTG Cuts at: 2266 2331 3787 4246 4294 2266 Size: 65 1456 459 48 5356 Fragments arranged by size: 5356 1456 459 65 48 SacI G_AGCT'C Cuts at: 3294 3294 Size: 7384 SacII CC_GC'GG Cuts at: 2380 2380 Size: 7384 SalI G'TCGA_C Cuts at: 1936 2639 4726 1936 Size: 703 2087 4594 Fragments arranged by size: 4594 2087 703 SbfI CC_TGCA'GG Cuts at: 4736 4736 Size: 7384 ScaI AGT'ACT Cuts at: 5632 7344 5632 Size: 1712 5672 SfcI C'TryA_G Cuts at: 826 1045 2580 4348 4732 5867 6548 6739 826 Size: 219 1535 1768 384 1135 681 191 1471 Fragments arranged by size: 1768 1535 1471 1135 681 384 219 191 SfoI GGC'GCC Cuts at: 2158 2185 2211 2158 Size: 27 26 7331 Fragments arranged by size: 7331 27 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7384 SgrAI Cr'CCGG_yG Cuts at: 1700 2186 1700 Size: 486 6898 SmaI CCC'GGG Cuts at: 1872 2021 2089 2162 2224 2251 4711 1872 Size: 149 68 73 62 27 2460 4545 Fragments arranged by size: 4545 2460 149 73 68 62 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3131 3421 3930 5488 6389 Size: 197 926 441 747 290 509 1558 901 Cuts at: 6389 6651 820 Size: 262 1553 Fragments arranged by size: 1558 1553 926 901 747 509 441 290 262 197 SnaBI TAC'GTA Cuts at: 493 5175 493 Size: 4682 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7384 SphI G_CATG'C Cuts at: 1132 4346 4742 1132 Size: 3214 396 3774 Fragments arranged by size: 3774 3214 396 SrfI GCCC'GGGC Cuts at: 2251 2251 Size: 7384 SspI AAT'ATT Cuts at: 5 52 5308 5 Size: 47 5256 2081 Fragments arranged by size: 5256 2081 47 StuI AGG'CCT Cuts at: 1517 3907 4669 1517 Size: 2390 762 4232 Fragments arranged by size: 4232 2390 762 StyI C'CwwG_G Cuts at: 513 1065 3405 4582 4823 513 Size: 552 2340 1177 241 3074 Fragments arranged by size: 3074 2340 1177 552 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2120 2624 4232 5411 5428 5581 5766 Size: 1255 540 504 1608 1179 17 153 185 Cuts at: 5766 6211 325 Size: 445 1498 Fragments arranged by size: 1608 1498 1255 1179 540 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3540 5630 7342 Size: 274 80 33 51 1443 1563 2090 1712 Cuts at: 7342 96 Size: 138 Fragments arranged by size: 2090 1712 1563 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2656 2688 3737 3769 7344 7376 2656 Size: 32 1049 32 3575 32 2664 Fragments arranged by size: 3575 2664 1049 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7384 XbaI T'CTAG_A Cuts at: 4720 4720 Size: 7384 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3509 3614 3509 Size: 105 7279 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7384 XmaI C'CCGG_G Cuts at: 1870 2019 2087 2160 2222 2249 4709 1870 Size: 149 68 73 62 27 2460 4545 Fragments arranged by size: 4545 2460 149 73 68 62 27 XmnI GAAnn'nnTTC Cuts at: 5513 5513 Size: 7384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5192 276 Size: 53 83 186 936 3658 2468 Fragments arranged by size: 3658 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI DraIII EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI SwaI