Restriction map for
pFN21AE0066
(Linear) (Six-base) MAPSORT of: KIEE0066.seq Check: 7417 from: 1 to: 2679
KIEE0066.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 0 635 677 692 2679
Size: 635 42 15 1987
Fragments arranged by size:
1987 635 42 15
Acc65I G'GTAC_C
Cuts at: 0 808 2679
Size: 808 1871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 703 1090 1783 2387 2627 2679
Size: 306 397 387 693 604 240 52
Fragments arranged by size:
693 604 397 387 306 240 52
AfeI AGC'GCT
Cuts at: 0 1816 2679
Size: 1816 863
AflII C'TTAA_G
Cuts at: 0 1126 2679
Size: 1126 1553
AflIII A'CryG_T
Cuts at: 0 527 2439 2441 2679
Size: 527 1912 2 238
Fragments arranged by size:
1912 527 238 2
AlwNI CAG_nnn'CTG
Cuts at: 0 490 2002 2103 2220 2679
Size: 490 1512 101 117 459
Fragments arranged by size:
1512 490 459 117 101
ApaI G_GGCC'C
Cuts at: 0 169 2679
Size: 169 2510
AvaI C'yCGr_G
Cuts at: 0 14 82 155 217 244 1833 2679
Size: 14 68 73 62 27 1589 846
Fragments arranged by size:
1589 846 73 68 62 27 14
BanI G'GyrC_C
Cuts at: 0 151 178 204 808 2679
Size: 151 27 26 604 1871
Fragments arranged by size:
1871 604 151 27 26
BanII G_rGCy'C
Cuts at: 0 169 317 1289 2576 2679
Size: 169 148 972 1287 103
Fragments arranged by size:
1287 972 169 148 103
BbeI G_GCGC'C
Cuts at: 0 155 182 208 2679
Size: 155 27 26 2471
Fragments arranged by size:
2471 155 27 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 425 1304 2375 2679
Size: 425 879 1071 304
Fragments arranged by size:
1071 879 425 304
BbvCI CC'TCA_GC
Cuts at: 0 1774 2233 2679
Size: 1774 459 446
Fragments arranged by size:
1774 459 446
BciVI GTATCCnnnnn_n'
Cuts at: 0 805 1073 2679
Size: 805 268 1606
Fragments arranged by size:
1606 805 268
BclI T'GATC_A
Cuts at: 0 2472 2679
Size: 2472 207
BglI GCCn_nnn'nGGC
Cuts at: 0 121 187 2006 2679
Size: 121 66 1819 673
Fragments arranged by size:
1819 673 121 66
BlpI GC'TnA_GC
Cuts at: 0 74 229 316 2679
Size: 74 155 87 2363
Fragments arranged by size:
2363 155 87 74
Bme1580I G_kGCm'C
Cuts at: 0 169 1619 2679
Size: 169 1450 1060
Fragments arranged by size:
1450 1060 169
BmgBI CAC'GTC
Cuts at: 0 639 2679
Size: 639 2040
BmrI ACTGGGnnnn_n'
Cuts at: 0 2284 2625 2679
Size: 2284 341 54
Fragments arranged by size:
2284 341 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 1889 1979 2112 2679
Size: 1444 445 90 133 567
Fragments arranged by size:
1444 567 445 133 90
Bpu10I CC'TnA_GC
Cuts at: 0 610 1097 1774 2233 2679
Size: 610 487 677 459 446
Fragments arranged by size:
677 610 487 459 446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1437 1910 2679
Size: 364 1073 473 769
Fragments arranged by size:
1073 769 473 364
BsaI GGTCTCn'nnnn_
Cuts at: 0 1997 2679
Size: 1997 682
BsaAI yAC'GTr
Cuts at: 0 2442 2679
Size: 2442 237
BsaHI Gr'CG_yC
Cuts at: 0 4 152 179 205 2679
Size: 4 148 27 26 2474
Fragments arranged by size:
2474 148 27 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 439 2115 2529 2679
Size: 325 114 1676 414 150
Fragments arranged by size:
1676 414 325 150 114
BseYI C'CCAG_C
Cuts at: 0 101 190 300 326 2075 2176 2679
Size: 101 89 110 26 1749 101 503
Fragments arranged by size:
1749 503 110 101 101 89 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2089 2679
Size: 2089 590
BsiEI CG_ry'CG
Cuts at: 0 116 2679
Size: 116 2563
BsiHKAI G_wGCw'C
Cuts at: 0 100 1289 1880 2679
Size: 100 1189 591 799
Fragments arranged by size:
1189 799 591 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1724 2679
Size: 1724 955
Bsp1286I G_dGCh'C
Cuts at: 0 100 169 317 1289 1619 1880 2576 2679
Size: 100 69 148 972 330 261 696 103
Fragments arranged by size:
972 696 330 261 148 103 100 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 2679
Size: 470 2209
BsrBI CCG'CTC
Cuts at: 0 149 2679
Size: 149 2530
BsrDI GCAATG_nn'
Cuts at: 0 1522 2354 2679
Size: 1522 832 325
Fragments arranged by size:
1522 832 325
BsrFI r'CCGG_y
Cuts at: 0 181 2089 2679
Size: 181 1908 590
Fragments arranged by size:
1908 590 181
BssHII G'CGCG_C
Cuts at: 0 401 450 2679
Size: 401 49 2229
Fragments arranged by size:
2229 401 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 805 2136 2679
Size: 805 1331 543
Fragments arranged by size:
1331 805 543
BstYI r'GATC_y
Cuts at: 0 1101 2282 2679
Size: 1101 1181 397
Fragments arranged by size:
1181 1101 397
BstZ17I GTA'TAC
Cuts at: 0 678 2679
Size: 678 2001
Bsu36I CC'TnA_GG
Cuts at: 0 655 880 2084 2096 2666 2679
Size: 655 225 1204 12 570 13
Fragments arranged by size:
1204 655 570 225 13 12
BtgI C'CryG_G
Cuts at: 0 372 2679
Size: 372 2307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 157 2166 2190 2679
Size: 157 2009 24 489
Fragments arranged by size:
2009 489 157 24
BtsI GCAGTG_nn'
Cuts at: 0 132 2052 2679
Size: 132 1920 627
Fragments arranged by size:
1920 627 132
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 2386 2679
Size: 9 2377 293
Fragments arranged by size:
2377 293 9
EaeI y'GGCC_r
Cuts at: 0 113 2679
Size: 113 2566
EagI C'GGCC_G
Cuts at: 0 113 2679
Size: 113 2566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 51 2679
Size: 34 17 2628
Fragments arranged by size:
2628 34 17
EcoICRI GAG'CTC
Cuts at: 0 1287 2679
Size: 1287 1392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 703 1090 1444 1783 1889 1979 2112
Size: 306 397 387 354 339 106 90 133
Cuts at: 2112 2387 2627 2679
Size: 275 240 52
Fragments arranged by size:
397 387 354 339 306 275 240 133
106 90 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1462 2679
Size: 1462 1217
EcoO109I rG'GnC_Cy
Cuts at: 0 165 384 1710 1767 1999 2679
Size: 165 219 1326 57 232 680
Fragments arranged by size:
1326 680 232 219 165 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1332 1431 2593 2679
Size: 1332 99 1162 86
Fragments arranged by size:
1332 1162 99 86
FseI GG_CCGG'CC
Cuts at: 0 2093 2679
Size: 2093 586
HaeII r_GCGC'y
Cuts at: 0 63 155 182 208 412 1365 1707 1818
Size: 63 92 27 26 204 953 342 111
Cuts at: 1818 2679
Size: 861
Fragments arranged by size:
953 861 342 204 111 92 63 27
26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 964 996 2649 2679
Size: 964 32 1653 30
Fragments arranged by size:
1653 964 32 30
HincII GTy'rAC
Cuts at: 0 636 2679
Size: 636 2043
HindIII A'AGCT_T
Cuts at: 0 2320 2478 2679
Size: 2320 158 201
Fragments arranged by size:
2320 201 158
KasI G'GCGC_C
Cuts at: 0 151 178 204 2679
Size: 151 27 26 2475
Fragments arranged by size:
2475 151 27 26
KpnI G_GTAC'C
Cuts at: 0 812 2679
Size: 812 1867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 2679
Size: 837 1842
MslI CAynn'nnrTG
Cuts at: 0 817 1438 1804 1855 2679
Size: 817 621 366 51 824
Fragments arranged by size:
824 817 621 366 51
MspA1I CmG'CkG
Cuts at: 0 261 326 374 1782 2241 2289 2679
Size: 261 65 48 1408 459 48 390
Fragments arranged by size:
1408 459 390 261 65 48 48
NaeI GCC'GGC
Cuts at: 0 183 2091 2679
Size: 183 1908 588
Fragments arranged by size:
1908 588 183
NarI GG'CG_CC
Cuts at: 0 152 179 205 2679
Size: 152 27 26 2474
Fragments arranged by size:
2474 152 27 26
NdeI CA'TA_TG
Cuts at: 0 1982 2679
Size: 1982 697
NgoMIV G'CCGG_C
Cuts at: 0 181 2089 2679
Size: 181 1908 590
Fragments arranged by size:
1908 590 181
NspI r_CATG'y
Cuts at: 0 531 2341 2397 2679
Size: 531 1810 56 282
Fragments arranged by size:
1810 531 282 56
PasI CC'CwG_GG
Cuts at: 0 1752 2259 2679
Size: 1752 507 420
Fragments arranged by size:
1752 507 420
PciI A'CATG_T
Cuts at: 0 527 2679
Size: 527 2152
PflMI CCAn_nnn'nTGG
Cuts at: 0 2002 2038 2624 2679
Size: 2002 36 586 55
Fragments arranged by size:
2002 586 55 36
PfoI T'CCnGG_A
Cuts at: 0 824 1263 2679
Size: 824 439 1416
Fragments arranged by size:
1416 824 439
PmlI CAC'GTG
Cuts at: 0 2442 2679
Size: 2442 237
PpuMI rG'GwC_Cy
Cuts at: 0 1710 1767 2679
Size: 1710 57 912
Fragments arranged by size:
1710 912 57
PspOMI G'GGCC_C
Cuts at: 0 165 2679
Size: 165 2514
PstI C_TGCA'G
Cuts at: 0 2347 2679
Size: 2347 332
PvuII CAG'CTG
Cuts at: 0 261 326 1782 2241 2289 2679
Size: 261 65 1456 459 48 390
Fragments arranged by size:
1456 459 390 261 65 48
SacI G_AGCT'C
Cuts at: 0 1289 2679
Size: 1289 1390
SacII CC_GC'GG
Cuts at: 0 375 2679
Size: 375 2304
SalI G'TCGA_C
Cuts at: 0 634 2679
Size: 634 2045
SfcI C'TryA_G
Cuts at: 0 575 2343 2679
Size: 575 1768 336
Fragments arranged by size:
1768 575 336
SfoI GGC'GCC
Cuts at: 0 153 180 206 2679
Size: 153 27 26 2473
Fragments arranged by size:
2473 153 27 26
SgrAI Cr'CCGG_yG
Cuts at: 0 181 2679
Size: 181 2498
SmaI CCC'GGG
Cuts at: 0 16 84 157 219 246 2679
Size: 16 68 73 62 27 2433
Fragments arranged by size:
2433 73 68 62 27 16
SmlI C'TyrA_G
Cuts at: 0 379 1126 1416 1925 2679
Size: 379 747 290 509 754
Fragments arranged by size:
754 747 509 379 290
SphI G_CATG'C
Cuts at: 0 2341 2679
Size: 2341 338
SrfI GCCC'GGGC
Cuts at: 0 246 2679
Size: 246 2433
StuI AGG'CCT
Cuts at: 0 1902 2664 2679
Size: 1902 762 15
Fragments arranged by size:
1902 762 15
StyI C'CwwG_G
Cuts at: 0 1400 2577 2679
Size: 1400 1177 102
Fragments arranged by size:
1400 1177 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 115 619 2227 2679
Size: 115 504 1608 452
Fragments arranged by size:
1608 504 452 115
TatI w'GTAC_w
Cuts at: 0 1535 2679
Size: 1535 1144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 651 683 1732 1764 2679
Size: 651 32 1049 32 915
Fragments arranged by size:
1049 915 651 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1504 1609 2679
Size: 1504 105 1070
Fragments arranged by size:
1504 1070 105
XmaI C'CCGG_G
Cuts at: 0 14 82 155 217 244 2679
Size: 14 68 73 62 27 2435
Fragments arranged by size:
2435 73 68 62 27 14
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DrdI EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SalI SfcI SfoI SgrAI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BfrBI BglII BmtI BplI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrGI BssSI BstAPI BstBI
BstEII ClaI CspCI DraI DraIII EarI EcoRI EcoRV
FspI FspAI HpaI MfeI MluI MscI NcoI NheI
NotI NruI NsiI PacI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SanDI SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI