(Circular) (Six-base) MAPSORT of: pFN21AE0065.seq Check: 1501 from: 1 to: 7795 pFN21AE0065 7795 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5605 278 Size: 53 83 186 936 4069 2468 Fragments arranged by size: 4069 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 5138 1937 Size: 1242 1959 4594 Fragments arranged by size: 4594 1959 1242 Acc65I G'GTAC_C Cuts at: 5116 5116 Size: 7795 AclI AA'CG_TT Cuts at: 1563 5922 6295 1563 Size: 4359 373 3063 Fragments arranged by size: 4359 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2858 3264 3531 5858 7242 7510 1632 Size: 1226 406 267 2327 1384 268 1917 Fragments arranged by size: 2327 1917 1384 1226 406 268 267 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7598 AflIII A'CryG_T Cuts at: 1203 2583 5005 6694 7671 1203 Size: 1380 2422 1689 977 1327 Fragments arranged by size: 2422 1689 1380 1327 977 AgeI A'CCGG_T Cuts at: 6626 6626 Size: 7795 AhdI GACnn_n'nnGTC Cuts at: 2587 3490 6524 2587 Size: 903 3034 3858 Fragments arranged by size: 3858 3034 903 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7795 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7795 AlwNI CAG_nnn'CTG Cuts at: 3049 5538 7110 3049 Size: 2489 1572 3734 Fragments arranged by size: 3734 2489 1572 ApaI G_GGCC'C Cuts at: 3523 5038 3523 Size: 1515 6280 ApaLI G'TGCA_C Cuts at: 5852 7008 5852 Size: 1156 6639 ApoI r'AATT_y Cuts at: 1493 2039 2132 2796 2830 3577 4609 4623 5104 Size: 546 93 664 34 747 1032 14 481 Cuts at: 5104 5330 5482 7591 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1032 747 664 546 481 226 152 93 34 14 AseI AT'TA_AT Cuts at: 160 1032 6349 160 Size: 872 5317 1606 Fragments arranged by size: 5317 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7795 AvaI C'yCGr_G Cuts at: 1870 1943 3066 4472 5120 1870 Size: 73 1123 1406 648 4545 Fragments arranged by size: 4545 1406 1123 648 73 BamHI G'GATC_C Cuts at: 5125 5492 5125 Size: 367 7428 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2379 4016 4467 5116 6571 Size: 325 217 627 592 1637 451 649 1455 Cuts at: 6571 618 Size: 1842 Fragments arranged by size: 1842 1637 1455 649 627 592 451 325 217 BanII G_rGCy'C Cuts at: 1391 2745 3523 3537 4864 5038 5114 1391 Size: 1354 778 14 1327 174 76 4072 Fragments arranged by size: 4072 1354 1327 778 174 76 14 BbeI G_GCGC'C Cuts at: 4471 4471 Size: 7795 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4744 928 Size: 971 2845 3979 Fragments arranged by size: 3979 2845 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3607 3641 3972 4006 5986 6020 1903 Size: 34 1670 34 331 34 1980 34 3678 Fragments arranged by size: 3678 1980 1670 331 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5687 6897 5687 Size: 1210 6585 BclI T'GATC_A Cuts at: 1548 1548 Size: 7795 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6406 136 Size: 107 122 71 631 605 4734 1525 Fragments arranged by size: 4734 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3023 3190 3320 3986 4479 4486 7790 3023 Size: 167 130 666 493 7 3304 3028 Fragments arranged by size: 3304 3028 666 493 167 130 7 BlpI GC'TnA_GC Cuts at: 2615 3629 5212 2615 Size: 1014 1583 5198 Fragments arranged by size: 5198 1583 1014 Bme1580I G_kGCm'C Cuts at: 1790 3478 3523 4931 5038 5856 7012 1790 Size: 1688 45 1408 107 818 1156 2573 Fragments arranged by size: 2573 1688 1408 1156 818 107 45 BmgBI CAC'GTC Cuts at: 1318 3677 4169 1318 Size: 2359 492 4944 Fragments arranged by size: 4944 2359 492 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3421 3531 3800 Size: 416 53 83 399 24 2004 110 269 Cuts at: 3800 5526 6484 442 Size: 1726 958 1753 Fragments arranged by size: 2004 1753 1726 958 416 399 269 110 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4440 1056 Size: 3384 4411 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4498 3977 Size: 521 7274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2661 3141 3951 3972 3991 4691 4864 4889 Size: 1422 480 810 21 19 700 173 25 Cuts at: 4889 4922 6455 1239 Size: 33 1533 2579 Fragments arranged by size: 2579 1533 1422 810 700 480 173 33 25 21 19 Bpu10I CC'TnA_GC Cuts at: 4640 4640 Size: 7795 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 3290 3910 4301 5920 6785 7083 2189 Size: 1101 620 391 1619 865 298 2901 Fragments arranged by size: 2901 1619 1101 865 620 391 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3086 3995 6458 882 Size: 617 1587 909 2463 2219 Fragments arranged by size: 2463 2219 1587 909 617 BsaAI yAC'GTr Cuts at: 493 1204 5586 493 Size: 711 4382 2702 Fragments arranged by size: 4382 2702 711 BsaBI GATnn'nnATC Cuts at: 7789 7789 Size: 7795 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4468 5602 5984 6619 Size: 53 83 186 936 2935 1134 382 635 Cuts at: 6619 275 Size: 1451 Fragments arranged by size: 2935 1451 1134 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2745 6228 6626 6900 7047 7458 2745 Size: 3483 398 274 147 411 3082 Fragments arranged by size: 3483 3082 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4126 4156 1178 Size: 30 165 30 2723 30 4817 Fragments arranged by size: 4817 2723 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7795 BseYI C'CCAG_C Cuts at: 2848 3368 3816 3945 4974 6998 7494 2848 Size: 520 448 129 1029 2024 496 3149 Fragments arranged by size: 3149 2024 1029 520 496 448 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3125 3747 1163 Size: 573 1389 622 5211 Fragments arranged by size: 5211 1389 622 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5245 6006 7034 1540 Size: 275 186 3244 761 1028 2301 Fragments arranged by size: 3244 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3732 5856 5941 7012 3732 Size: 2124 85 1071 4515 Fragments arranged by size: 4515 2124 1071 85 BsmI GAATG_Cn' Cuts at: 3835 4268 5080 5306 5399 3835 Size: 433 812 226 93 6231 Fragments arranged by size: 6231 812 433 226 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2745 3478 3523 3537 3732 4864 4931 Size: 399 955 733 45 14 195 1132 67 Cuts at: 4931 5038 5114 5856 5941 7012 1391 Size: 107 76 742 85 1071 2174 Fragments arranged by size: 2174 1132 1071 955 742 733 399 195 107 85 76 67 45 14 BspEI T'CCGG_A Cuts at: 7458 7458 Size: 7795 BspHI T'CATG_A Cuts at: 4063 5684 7414 4063 Size: 1621 1730 4444 Fragments arranged by size: 4444 1730 1621 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5150 844 Size: 4306 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5682 1394 Size: 429 3859 3507 Fragments arranged by size: 3859 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6290 6464 65 Size: 1183 5042 174 1396 Fragments arranged by size: 5042 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2990 6439 6626 1700 Size: 110 1180 3449 187 2869 Fragments arranged by size: 3449 2869 1180 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7795 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7795 BssSI C'ACGA_G Cuts at: 3616 5855 6867 3616 Size: 2239 1012 4544 Fragments arranged by size: 4544 2239 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7795 BstBI TT'CG_AA Cuts at: 6599 6599 Size: 7795 BstEII G'GTnAC_C Cuts at: 1184 4820 1184 Size: 3636 4159 BstXI CCAn_nnnn'nTGG Cuts at: 3877 4464 4652 3877 Size: 587 188 7020 Fragments arranged by size: 7020 587 188 BstYI r'GATC_y Cuts at: 1972 2046 3023 3190 3320 3986 4479 4486 4570 Size: 74 977 167 130 666 493 7 84 Cuts at: 4570 5125 5492 5877 5894 7346 7432 7444 7790 Size: 555 367 385 17 1452 86 12 346 Cuts at: 7790 1972 Size: 1977 Fragments arranged by size: 1977 1452 977 666 555 493 385 367 346 167 130 86 84 74 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2577 2737 3469 4277 513 Size: 552 1512 160 732 808 4031 Fragments arranged by size: 4031 1512 808 732 552 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2634 487 Size: 683 56 1408 5648 Fragments arranged by size: 5648 1408 683 56 BtsI GCAGTG_nn' Cuts at: 3137 3374 4517 5311 6105 6125 3137 Size: 237 1143 794 794 20 4807 Fragments arranged by size: 4807 1143 794 794 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7795 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7760 DraI TTT'AAA Cuts at: 5099 5099 Size: 7795 DraIII CAC_nnn'GTG Cuts at: 3482 4845 3482 Size: 1363 6432 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6802 809 Size: 507 5486 1802 Fragments arranged by size: 5486 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5242 6131 7760 Size: 54 1425 50 275 18 3412 889 1629 Cuts at: 7760 8 Size: 43 Fragments arranged by size: 3412 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5242 1537 Size: 275 3430 4090 Fragments arranged by size: 4090 3430 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4027 4546 5725 1353 Size: 2674 519 1179 3423 Fragments arranged by size: 3423 2674 1179 519 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6382 6756 6902 387 Size: 1064 138 4793 374 146 1280 Fragments arranged by size: 4793 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2661 2858 3141 3264 3531 3951 3972 Size: 393 1029 197 283 123 267 420 21 Cuts at: 3972 3991 4691 4864 4889 4922 5858 6455 7242 Size: 19 700 173 25 33 936 597 787 Cuts at: 7242 7510 1239 Size: 268 1524 Fragments arranged by size: 1524 1029 936 787 700 597 420 393 283 268 267 197 173 123 33 25 21 19 EcoNI CCTnn'n_nnAGG Cuts at: 3282 3448 4643 3282 Size: 166 1195 6434 Fragments arranged by size: 6434 1195 166 EcoO109I rG'GnC_Cy Cuts at: 3519 5034 3519 Size: 1515 6280 EcoRI G'AATT_C Cuts at: 3577 5104 5482 3577 Size: 1527 378 5890 Fragments arranged by size: 5890 1527 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2278 2964 3021 2278 Size: 686 57 7052 Fragments arranged by size: 7052 686 57 FspI TGC'GCA Cuts at: 4523 6301 4523 Size: 1778 6017 HaeII r_GCGC'y Cuts at: 1678 2562 4471 6942 1678 Size: 884 1909 2471 2531 Fragments arranged by size: 2531 2471 1909 884 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2573 2605 3476 3508 3978 Size: 32 135 32 1001 32 871 32 470 Cuts at: 3978 4010 4058 4090 4471 4503 6436 6468 6510 Size: 32 48 32 381 32 1933 32 42 Cuts at: 6510 6542 1373 Size: 32 2626 Fragments arranged by size: 2626 1933 1001 871 470 381 135 48 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2761 4203 5139 5385 669 Size: 988 281 823 1442 936 246 3079 Fragments arranged by size: 3079 1442 988 936 823 281 246 HindIII A'AGCT_T Cuts at: 748 2711 3097 3886 748 Size: 1963 386 789 4657 Fragments arranged by size: 4657 1963 789 386 HpaI GTT'AAC Cuts at: 2761 5385 2761 Size: 2624 5171 KasI G'GCGC_C Cuts at: 4467 4467 Size: 7795 KpnI G_GTAC'C Cuts at: 5120 5120 Size: 7795 MfeI C'AATT_G Cuts at: 5394 5394 Size: 7795 MluI A'CGCG_T Cuts at: 7671 7671 Size: 7795 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2225 2823 4416 6909 7093 1305 Size: 920 598 1593 2493 184 2007 Fragments arranged by size: 2493 2007 1593 920 598 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5973 Fragments arranged by size: 5973 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2388 2442 4077 4922 5073 5753 6112 Size: 648 1222 54 1635 845 151 680 359 Cuts at: 6112 6271 518 Size: 159 2042 Fragments arranged by size: 2042 1635 1222 845 680 648 359 159 151 54 MspA1I CmG'CkG Cuts at: 1606 2756 2852 4711 4965 5211 5888 7036 7281 Size: 1150 96 1859 254 246 677 1148 245 Cuts at: 7281 1606 Size: 2120 Fragments arranged by size: 2120 1859 1150 1148 677 254 246 245 96 NaeI GCC'GGC Cuts at: 2992 2992 Size: 7795 NarI GG'CG_CC Cuts at: 4468 4468 Size: 7795 NcoI C'CATG_G Cuts at: 513 1065 2737 3469 4277 513 Size: 552 1672 732 808 4031 Fragments arranged by size: 4031 1672 808 732 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7795 NgoMIV G'CCGG_C Cuts at: 2990 2990 Size: 7795 NheI G'CTAG_C Cuts at: 1052 4436 1052 Size: 3384 4411 NotI GC'GGCC_GC Cuts at: 5242 5242 Size: 7795 NspI r_CATG'y Cuts at: 1132 1229 2587 5153 6698 1132 Size: 97 1358 2566 1545 2229 Fragments arranged by size: 2566 2229 1545 1358 97 PasI CC'CwG_GG Cuts at: 1348 3290 1348 Size: 1942 5853 PciI A'CATG_T Cuts at: 2583 6694 2583 Size: 4111 3684 PflMI CCAn_nnn'nTGG Cuts at: 1108 2529 4721 1108 Size: 1421 2192 4182 Fragments arranged by size: 4182 2192 1421 PfoI T'CCnGG_A Cuts at: 2638 2638 Size: 7795 PmeI GTTT'AAAC Cuts at: 5099 5099 Size: 7795 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3323 3355 5864 5896 7377 7409 3323 Size: 32 2509 32 1481 32 3709 Fragments arranged by size: 3709 2509 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3078 3675 1613 Size: 1465 597 5733 Fragments arranged by size: 5733 1465 597 PsiI TTA'TAA Cuts at: 5365 5365 Size: 7795 PspOMI G'GGCC_C Cuts at: 3519 5034 3519 Size: 1515 6280 PstI C_TGCA'G Cuts at: 830 3381 4494 5147 830 Size: 2551 1113 653 3478 Fragments arranged by size: 3478 2551 1113 653 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7795 PvuII CAG'CTG Cuts at: 2756 2852 4711 4965 2756 Size: 96 1859 254 5586 Fragments arranged by size: 5586 1859 254 96 SalI G'TCGA_C Cuts at: 1936 5137 1936 Size: 3201 4594 SbfI CC_TGCA'GG Cuts at: 5147 5147 Size: 7795 ScaI AGT'ACT Cuts at: 3001 4902 6043 7755 3001 Size: 1901 1141 1712 3041 Fragments arranged by size: 3041 1901 1712 1141 SfcI C'TryA_G Cuts at: 826 1045 2719 3377 3448 4490 5143 6278 6959 Size: 219 1674 658 71 1042 653 1135 681 Cuts at: 6959 7150 826 Size: 191 1471 Fragments arranged by size: 1674 1471 1135 1042 681 658 653 219 191 71 SfoI GGC'GCC Cuts at: 4469 4469 Size: 7795 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7795 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7795 SmaI CCC'GGG Cuts at: 1872 3068 5122 1872 Size: 1196 2054 4545 Fragments arranged by size: 4545 2054 1196 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 3305 3889 4316 5899 6800 Size: 197 926 261 1101 584 427 1583 901 Cuts at: 6800 7062 820 Size: 262 1553 Fragments arranged by size: 1583 1553 1101 926 901 584 427 262 261 197 SnaBI TAC'GTA Cuts at: 493 5586 493 Size: 5093 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7795 SphI G_CATG'C Cuts at: 1132 5153 1132 Size: 4021 3774 SspI AAT'ATT Cuts at: 5 52 3265 4297 5719 5 Size: 47 3213 1032 1422 2081 Fragments arranged by size: 3213 2081 1422 1032 47 StuI AGG'CCT Cuts at: 1517 3060 4649 1517 Size: 1543 1589 4663 Fragments arranged by size: 4663 1589 1543 StyI C'CwwG_G Cuts at: 513 1065 2050 2737 2775 2791 3456 3469 3870 Size: 552 985 687 38 16 665 13 401 Cuts at: 3870 4130 4277 5234 513 Size: 260 147 957 3074 Fragments arranged by size: 3074 985 957 687 665 552 401 260 147 38 16 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2526 3982 5822 5839 5992 6177 6622 Size: 1255 946 1456 1840 17 153 185 445 Cuts at: 6622 325 Size: 1498 Fragments arranged by size: 1840 1498 1456 1255 946 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2083 2259 2999 Size: 274 80 33 51 1443 106 176 740 Cuts at: 2999 4900 6041 7753 96 Size: 1901 1141 1712 138 Fragments arranged by size: 1901 1712 1443 1141 740 274 176 138 106 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2562 2594 4547 4579 4926 4958 7755 7787 2562 Size: 32 1953 32 347 32 2797 32 2570 Fragments arranged by size: 2797 2570 1953 347 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7795 XbaI T'CTAG_A Cuts at: 5131 5131 Size: 7795 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7795 XmaI C'CCGG_G Cuts at: 1870 3066 5120 1870 Size: 1196 2054 4545 Fragments arranged by size: 4545 2054 1196 XmnI GAAnn'nnTTC Cuts at: 2238 2714 5924 2238 Size: 476 3210 4109 Fragments arranged by size: 4109 3210 476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5603 276 Size: 53 83 186 936 4069 2468 Fragments arranged by size: 4069 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI