(Circular) (Six-base) MAPSORT of: pFN21AE0057.seq Check: 8775 from: 1 to: 8242 pFN21AE0057 8242 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3082 5193 3082 Size: 2111 6131 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6052 278 Size: 53 83 186 936 4516 2468 Fragments arranged by size: 4516 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5585 1937 Size: 3648 4594 Acc65I G'GTAC_C Cuts at: 5563 5563 Size: 8242 AclI AA'CG_TT Cuts at: 1563 6369 6742 1563 Size: 4806 373 3063 Fragments arranged by size: 4806 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2098 2403 2937 3046 3431 3464 3776 Size: 401 65 305 534 109 385 33 312 Cuts at: 3776 3836 3944 5162 5458 5525 6305 7689 7957 Size: 60 108 1218 296 67 780 1384 268 Cuts at: 7957 1632 Size: 1917 Fragments arranged by size: 1917 1384 1218 780 534 401 385 312 305 296 268 109 108 67 65 60 33 AfeI AGC'GCT Cuts at: 3767 3833 3767 Size: 66 8176 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8045 AflIII A'CryG_T Cuts at: 1203 7141 8118 1203 Size: 5938 977 1327 Fragments arranged by size: 5938 1327 977 AgeI A'CCGG_T Cuts at: 7073 7073 Size: 8242 AhdI GACnn_n'nnGTC Cuts at: 6971 6971 Size: 8242 AleI CACnn'nnGTG Cuts at: 1166 2571 1166 Size: 1405 6837 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2660 5454 1184 Size: 1476 2794 3972 Fragments arranged by size: 3972 2794 1476 AlwNI CAG_nnn'CTG Cuts at: 3287 3409 4056 4144 4217 4305 5185 5329 5985 Size: 122 647 88 73 88 880 144 656 Cuts at: 5985 7557 3287 Size: 1572 3972 Fragments arranged by size: 3972 1572 880 656 647 144 122 88 88 73 ApaI G_GGCC'C Cuts at: 4611 4611 Size: 8242 ApaLI G'TGCA_C Cuts at: 6299 7455 6299 Size: 1156 7086 ApoI r'AATT_y Cuts at: 1493 3290 3443 3977 5551 5777 5929 8038 1493 Size: 1797 153 534 1574 226 152 2109 1697 Fragments arranged by size: 2109 1797 1697 1574 534 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 6796 160 Size: 872 5764 1606 Fragments arranged by size: 5764 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8242 AvaI C'yCGr_G Cuts at: 1870 1943 2943 3983 5567 1870 Size: 73 1000 1040 1584 4545 Fragments arranged by size: 4545 1584 1040 1000 73 BamHI G'GATC_C Cuts at: 2486 4457 5572 5939 2486 Size: 1971 1115 367 4789 Fragments arranged by size: 4789 1971 1115 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2937 3094 3235 4540 4600 Size: 325 217 627 1150 157 141 1305 60 Cuts at: 4600 4916 5563 7018 618 Size: 316 647 1455 1842 Fragments arranged by size: 1842 1455 1305 1150 647 627 325 316 217 157 141 60 BanII G_rGCy'C Cuts at: 1391 2051 2383 3990 4211 4611 4640 5391 5561 Size: 660 332 1607 221 400 29 751 170 Cuts at: 5561 1391 Size: 4072 Fragments arranged by size: 4072 1607 751 660 400 332 221 170 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2316 2550 3166 3253 928 Size: 971 417 234 616 87 5917 Fragments arranged by size: 5917 971 616 417 234 87 BbvCI CC'TCA_GC Cuts at: 3534 3625 3534 Size: 91 8151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6433 6467 1903 Size: 34 4496 34 3678 Fragments arranged by size: 4496 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3455 6134 7344 3455 Size: 2679 1210 4353 Fragments arranged by size: 4353 2679 1210 BclI T'GATC_A Cuts at: 1548 2629 1548 Size: 1081 7161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5456 6853 136 Size: 107 122 71 631 605 3784 1397 1525 Fragments arranged by size: 3784 1525 1397 631 605 122 107 71 BglII A'GATC_T Cuts at: 2856 3048 3808 8237 2856 Size: 192 760 4429 2861 Fragments arranged by size: 4429 2861 760 192 BlpI GC'TnA_GC Cuts at: 2823 3309 4311 5659 2823 Size: 486 1002 1348 5406 Fragments arranged by size: 5406 1348 1002 486 Bme1580I G_kGCm'C Cuts at: 1790 2942 4036 4603 4611 4921 6303 7459 1790 Size: 1152 1094 567 8 310 1382 1156 2573 Fragments arranged by size: 2573 1382 1156 1152 1094 567 310 8 BmgBI CAC'GTC Cuts at: 1318 3514 1318 Size: 2196 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2565 2680 3740 Size: 416 53 83 399 24 1148 115 1060 Cuts at: 3740 5973 6931 442 Size: 2233 958 1753 Fragments arranged by size: 2233 1753 1148 1060 958 416 399 115 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8242 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3448 4439 5157 3448 Size: 991 718 6533 Fragments arranged by size: 6533 991 718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2490 2792 3418 3952 5000 5052 5110 5351 Size: 1251 302 626 534 1048 52 58 241 Cuts at: 5351 6902 1239 Size: 1551 2579 Fragments arranged by size: 2579 1551 1251 1048 626 534 302 241 58 52 Bpu10I CC'TnA_GC Cuts at: 2653 2844 3534 3625 3704 4069 5088 2653 Size: 191 690 91 79 365 1019 5807 Fragments arranged by size: 5807 1019 690 365 191 91 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2042 2221 4338 4959 6367 7232 7530 2042 Size: 179 2117 621 1408 865 298 2754 Fragments arranged by size: 2754 2117 1408 865 621 298 179 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4825 4861 6905 882 Size: 617 3326 36 2044 2219 Fragments arranged by size: 3326 2219 2044 617 36 BsaAI yAC'GTr Cuts at: 493 1204 6033 493 Size: 711 4829 2702 Fragments arranged by size: 4829 2702 711 BsaBI GATnn'nnATC Cuts at: 3677 4456 8236 3677 Size: 779 3780 3683 Fragments arranged by size: 3780 3683 779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6049 6431 7066 275 Size: 53 83 186 936 4516 382 635 1451 Fragments arranged by size: 4516 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6675 7073 7347 7494 7905 6675 Size: 398 274 147 411 7012 Fragments arranged by size: 7012 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3035 3065 4474 4504 1178 Size: 30 165 30 1632 30 1409 30 4916 Fragments arranged by size: 4916 1632 1409 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2199 2743 3043 3776 3853 4950 1186 Size: 1013 544 300 733 77 1097 4478 Fragments arranged by size: 4478 1097 1013 733 544 300 77 BseYI C'CCAG_C Cuts at: 2050 3015 3215 3797 4000 4098 4461 4867 4996 Size: 965 200 582 203 98 363 406 129 Cuts at: 4996 5050 5322 5384 7445 7941 2050 Size: 54 272 62 2061 496 2351 Fragments arranged by size: 2351 2061 965 582 496 406 363 272 203 200 129 98 62 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2951 1163 Size: 573 1215 6454 Fragments arranged by size: 6454 1215 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3741 4849 5692 6453 7481 1540 Size: 275 186 1740 1108 843 761 1028 2301 Fragments arranged by size: 2301 1740 1108 1028 843 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2702 3990 4070 4312 6303 6388 7459 2702 Size: 1288 80 242 1991 85 1071 3485 Fragments arranged by size: 3485 1991 1288 1071 242 85 80 BsmI GAATG_Cn' Cuts at: 5753 5846 5753 Size: 93 8149 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2383 2702 2942 3990 4036 4070 Size: 399 261 332 319 240 1048 46 34 Cuts at: 4070 4211 4312 4603 4611 4640 4921 5391 5561 Size: 141 101 291 8 29 281 470 170 Cuts at: 5561 6303 6388 7459 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1048 742 470 399 332 319 291 281 261 240 170 141 101 85 46 34 29 8 BspEI T'CCGG_A Cuts at: 7905 7905 Size: 8242 BspHI T'CATG_A Cuts at: 6131 7861 6131 Size: 1730 6512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 3082 5193 5597 844 Size: 1352 886 2111 404 3489 Fragments arranged by size: 3489 2111 1352 886 404 BsrBI CCG'CTC Cuts at: 1394 1823 2026 4084 6129 1394 Size: 429 203 2058 2045 3507 Fragments arranged by size: 3507 2058 2045 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 6737 6911 65 Size: 1183 5489 174 1396 Fragments arranged by size: 5489 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2535 6886 7073 1700 Size: 110 725 4351 187 2869 Fragments arranged by size: 4351 2869 725 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8242 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8242 BssSI C'ACGA_G Cuts at: 6302 7314 6302 Size: 1012 7230 BstAPI GCAn_nnn'nTGC Cuts at: 794 2919 4690 5447 794 Size: 2125 1771 757 3589 Fragments arranged by size: 3589 2125 1771 757 BstBI TT'CG_AA Cuts at: 7046 7046 Size: 8242 BstEII G'GTnAC_C Cuts at: 1184 3415 1184 Size: 2231 6011 BstXI CCAn_nnnn'nTGG Cuts at: 3787 4255 3787 Size: 468 7774 BstYI r'GATC_y Cuts at: 1972 2452 2486 2593 2790 2856 3048 3672 3808 Size: 480 34 107 197 66 192 624 136 Cuts at: 3808 4457 5572 5939 6324 6341 7793 7879 7891 Size: 649 1115 367 385 17 1452 86 12 Cuts at: 7891 8237 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1115 649 624 480 385 367 346 197 192 136 107 86 66 34 17 12 BtgI C'CryG_G Cuts at: 513 1065 3741 4128 4752 513 Size: 552 2676 387 624 4003 Fragments arranged by size: 4003 2676 624 552 387 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4544 5506 487 Size: 683 56 3318 962 3223 Fragments arranged by size: 3318 3223 962 683 56 BtsI GCAGTG_nn' Cuts at: 2245 2647 2887 2939 3086 4278 5448 5758 6552 Size: 402 240 52 147 1192 1170 310 794 Cuts at: 6552 6572 2245 Size: 20 3915 Fragments arranged by size: 3915 1192 1170 794 402 310 240 147 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8207 DraI TTT'AAA Cuts at: 5546 5546 Size: 8242 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4839 7249 809 Size: 507 3523 2410 1802 Fragments arranged by size: 3523 2410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2076 3492 Size: 54 1425 50 275 18 197 49 1416 Cuts at: 3492 3738 3840 4275 4846 5689 6578 8207 8 Size: 246 102 435 571 843 889 1629 43 Fragments arranged by size: 1629 1425 1416 889 843 571 435 275 246 197 102 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3738 4846 5689 1537 Size: 275 1926 1108 843 4090 Fragments arranged by size: 4090 1926 1108 843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3061 3500 4737 5234 5307 6172 1353 Size: 1708 439 1237 497 73 865 3423 Fragments arranged by size: 3423 1708 1237 865 497 439 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6829 7203 7349 387 Size: 1064 138 5240 374 146 1280 Fragments arranged by size: 5240 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3988 3988 Size: 8242 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2098 2403 2490 2792 2937 3046 Size: 393 401 65 305 87 302 145 109 Cuts at: 3046 3418 3431 3464 3776 3836 3944 3952 5000 Size: 372 13 33 312 60 108 8 1048 Cuts at: 5000 5052 5110 5162 5351 5458 5525 6305 6902 Size: 52 58 52 189 107 67 780 597 Cuts at: 6902 7689 7957 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1048 787 780 597 401 393 372 312 305 302 268 189 145 109 108 107 87 67 65 60 58 52 52 33 13 8 EcoNI CCTnn'n_nnAGG Cuts at: 2203 3707 3791 4965 2203 Size: 1504 84 1174 5480 Fragments arranged by size: 5480 1504 1174 84 EcoO109I rG'GnC_Cy Cuts at: 2158 2947 4141 4193 4214 4477 4568 4608 4841 Size: 789 1194 52 21 263 91 40 233 Cuts at: 4841 4909 4970 5434 2158 Size: 68 61 464 4966 Fragments arranged by size: 4966 1194 789 464 263 233 91 68 61 52 40 21 EcoRI G'AATT_C Cuts at: 3290 5551 5929 3290 Size: 2261 378 5603 Fragments arranged by size: 5603 2261 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3436 3494 4671 5120 5263 3436 Size: 58 1177 449 143 6415 Fragments arranged by size: 6415 1177 449 143 58 FspI TGC'GCA Cuts at: 6748 6748 Size: 8242 HaeII r_GCGC'y Cuts at: 1678 3769 3835 5258 7389 1678 Size: 2091 66 1423 2131 2531 Fragments arranged by size: 2531 2131 2091 1423 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3035 3067 4427 4459 4714 Size: 32 135 32 1463 32 1360 32 255 Cuts at: 4714 4746 5216 5248 6883 6915 6957 6989 1373 Size: 32 470 32 1635 32 42 32 2626 Fragments arranged by size: 2626 1635 1463 1360 470 255 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5586 5832 669 Size: 988 281 3648 246 3079 Fragments arranged by size: 3648 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3372 3452 4882 5422 748 Size: 2624 80 1430 540 3568 Fragments arranged by size: 3568 2624 1430 540 80 HpaI GTT'AAC Cuts at: 5832 5832 Size: 8242 KpnI G_GTAC'C Cuts at: 5567 5567 Size: 8242 MfeI C'AATT_G Cuts at: 5841 5841 Size: 8242 MluI A'CGCG_T Cuts at: 8118 8118 Size: 8242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5287 7356 7540 1305 Size: 3982 2069 184 2007 Fragments arranged by size: 3982 2069 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 3494 3842 4277 10 Size: 54 1425 343 246 1416 348 435 3975 Fragments arranged by size: 3975 1425 1416 435 348 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2571 4283 6200 6559 6718 518 Size: 648 1405 1712 1917 359 159 2042 Fragments arranged by size: 2042 1917 1712 1405 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2731 2916 3308 5658 6335 7483 7728 Size: 427 698 185 392 2350 677 1148 245 Cuts at: 7728 1606 Size: 2120 Fragments arranged by size: 2350 2120 1148 698 677 427 392 245 185 NcoI C'CATG_G Cuts at: 513 1065 4128 4752 513 Size: 552 3063 624 4003 Fragments arranged by size: 4003 3063 624 552 NdeI CA'TA_TG Cuts at: 387 3030 387 Size: 2643 5599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8242 NotI GC'GGCC_GC Cuts at: 5689 5689 Size: 8242 NspI r_CATG'y Cuts at: 1132 1229 2293 4000 4761 5450 5600 7145 1132 Size: 97 1064 1707 761 689 150 1545 2229 Fragments arranged by size: 2229 1707 1545 1064 761 689 150 97 PasI CC'CwG_GG Cuts at: 1348 2482 4264 4299 4640 4718 5004 1348 Size: 1134 1782 35 341 78 286 4586 Fragments arranged by size: 4586 1782 1134 341 286 78 35 PciI A'CATG_T Cuts at: 7141 7141 Size: 8242 PflMI CCAn_nnn'nTGG Cuts at: 1108 4946 5329 1108 Size: 3838 383 4021 Fragments arranged by size: 4021 3838 383 PfoI T'CCnGG_A Cuts at: 4982 5155 4982 Size: 173 8069 PmeI GTTT'AAAC Cuts at: 5546 5546 Size: 8242 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6311 6343 7824 7856 6311 Size: 32 1481 32 6697 Fragments arranged by size: 6697 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2947 4141 4193 4477 4568 4841 4909 4970 Size: 789 1194 52 284 91 273 68 61 Cuts at: 4970 2158 Size: 5430 Fragments arranged by size: 5430 1194 789 284 273 91 68 61 52 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8242 PsiI TTA'TAA Cuts at: 5812 5812 Size: 8242 PspOMI G'GGCC_C Cuts at: 4607 4607 Size: 8242 PstI C_TGCA'G Cuts at: 830 3093 3318 3584 4140 5175 5594 830 Size: 2263 225 266 556 1035 419 3478 Fragments arranged by size: 3478 2263 1035 556 419 266 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8242 PvuII CAG'CTG Cuts at: 2033 2731 2916 3308 2033 Size: 698 185 392 6967 Fragments arranged by size: 6967 698 392 185 SacI G_AGCT'C Cuts at: 3990 3990 Size: 8242 SalI G'TCGA_C Cuts at: 1936 5584 1936 Size: 3648 4594 SanDI GG'GwC_CC Cuts at: 2158 2947 4568 4909 2158 Size: 789 1621 341 5491 Fragments arranged by size: 5491 1621 789 341 SapI GCTCTTCn'nnn_ Cuts at: 5234 5234 Size: 8242 SbfI CC_TGCA'GG Cuts at: 5594 5594 Size: 8242 ScaI AGT'ACT Cuts at: 6490 8202 6490 Size: 1712 6530 SexAI A'CCwGG_T Cuts at: 4473 4473 Size: 8242 SfcI C'TryA_G Cuts at: 826 1045 3089 3314 3580 3916 4136 4228 4594 Size: 219 2044 225 266 336 220 92 366 Cuts at: 4594 5171 5464 5590 6725 7406 7597 826 Size: 577 293 126 1135 681 191 1471 Fragments arranged by size: 2044 1471 1135 681 577 366 336 293 266 225 220 219 191 126 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8242 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8242 SmaI CCC'GGG Cuts at: 1872 5569 1872 Size: 3697 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 2200 4353 4974 6346 7247 Size: 197 926 78 179 2153 621 1372 901 Cuts at: 7247 7509 820 Size: 262 1553 Fragments arranged by size: 2153 1553 1372 926 901 621 262 197 179 78 SnaBI TAC'GTA Cuts at: 493 6033 493 Size: 5540 2702 SpeI A'CTAG_T Cuts at: 152 2837 152 Size: 2685 5557 SphI G_CATG'C Cuts at: 1132 2293 4761 5450 5600 1132 Size: 1161 2468 689 150 3774 Fragments arranged by size: 3774 2468 1161 689 150 SspI AAT'ATT Cuts at: 5 52 2139 6166 5 Size: 47 2087 4027 2081 Fragments arranged by size: 4027 2087 2081 47 StuI AGG'CCT Cuts at: 1517 2658 3323 3575 3590 4651 1517 Size: 1141 665 252 15 1061 5108 Fragments arranged by size: 5108 1141 1061 665 252 15 StyI C'CwwG_G Cuts at: 513 1065 3324 3540 3665 4128 4493 4499 4536 Size: 552 2259 216 125 463 365 6 37 Cuts at: 4536 4752 5681 513 Size: 216 929 3074 Fragments arranged by size: 3074 2259 929 552 463 365 216 216 125 37 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2699 4980 6269 6286 6439 6624 7069 Size: 1255 1119 2281 1289 17 153 185 445 Cuts at: 7069 325 Size: 1498 Fragments arranged by size: 2281 1498 1289 1255 1119 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2994 6488 8200 Size: 274 80 33 51 1443 1017 3494 1712 Cuts at: 8200 96 Size: 138 Fragments arranged by size: 3494 1712 1443 1017 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3893 3925 8202 8234 3893 Size: 32 4277 32 3901 Fragments arranged by size: 4277 3901 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8242 XbaI T'CTAG_A Cuts at: 5578 5578 Size: 8242 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8242 XmaI C'CCGG_G Cuts at: 1870 5567 1870 Size: 3697 4545 XmnI GAAnn'nnTTC Cuts at: 2396 6371 2396 Size: 3975 4267 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6050 276 Size: 53 83 186 936 4516 2468 Fragments arranged by size: 4516 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI