(Circular) (Six-base) MAPSORT of: pFN21AE0053.seq Check: 2912 from: 1 to: 9409 pFN21AE0053 9409 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2919 6219 7219 278 Size: 53 83 186 936 1383 3300 1000 2468 Fragments arranged by size: 3300 2468 1383 1000 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5304 6071 6752 1937 Size: 3367 767 681 4594 Fragments arranged by size: 4594 3367 767 681 Acc65I G'GTAC_C Cuts at: 2529 6730 2529 Size: 4201 5208 AclI AA'CG_TT Cuts at: 1563 7536 7909 1563 Size: 5973 373 3063 Fragments arranged by size: 5973 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2302 2980 5139 5741 5919 6293 7472 8856 Size: 670 678 2159 602 178 374 1179 1384 Cuts at: 8856 9124 1632 Size: 268 1917 Fragments arranged by size: 2159 1917 1384 1179 678 670 602 374 268 178 AflII C'TTAA_G Cuts at: 820 1017 3203 5728 820 Size: 197 2186 2525 4501 Fragments arranged by size: 4501 2525 2186 197 AflIII A'CryG_T Cuts at: 1203 8308 9285 1203 Size: 7105 977 1327 Fragments arranged by size: 7105 1327 977 AgeI A'CCGG_T Cuts at: 8240 8240 Size: 9409 AhdI GACnn_n'nnGTC Cuts at: 8138 8138 Size: 9409 AleI CACnn'nnGTG Cuts at: 1166 2459 2552 2819 1166 Size: 1293 93 267 7756 Fragments arranged by size: 7756 1293 267 93 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2345 2459 2837 3535 1184 Size: 1161 114 378 698 7058 Fragments arranged by size: 7058 1161 698 378 114 AlwNI CAG_nnn'CTG Cuts at: 2662 3914 4110 6212 7152 8724 2662 Size: 1252 196 2102 940 1572 3347 Fragments arranged by size: 3347 2102 1572 1252 940 196 ApaLI G'TGCA_C Cuts at: 3445 7466 8622 3445 Size: 4021 1156 4232 Fragments arranged by size: 4232 4021 1156 ApoI r'AATT_y Cuts at: 1493 3223 3351 3528 3748 4163 4336 4590 5588 Size: 1730 128 177 220 415 173 254 998 Cuts at: 5588 5926 6509 6545 6573 6718 6944 7096 9205 Size: 338 583 36 28 145 226 152 2109 Cuts at: 9205 1493 Size: 1697 Fragments arranged by size: 2109 1730 1697 998 583 415 338 254 226 220 177 173 152 145 128 36 28 AseI AT'TA_AT Cuts at: 160 1032 7963 160 Size: 872 6931 1606 Fragments arranged by size: 6931 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9409 AvaI C'yCGr_G Cuts at: 1870 1943 2306 2360 4068 6734 1870 Size: 73 363 54 1708 2666 4545 Fragments arranged by size: 4545 2666 1708 363 73 54 BamHI G'GATC_C Cuts at: 4241 5894 6739 7106 4241 Size: 1653 845 367 6544 Fragments arranged by size: 6544 1653 845 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2284 2399 2453 2529 Size: 325 217 627 239 258 115 54 76 Cuts at: 2529 2667 3146 3426 6730 8185 618 Size: 138 479 280 3304 1455 1842 Fragments arranged by size: 3304 1842 1455 627 479 325 280 258 239 217 138 115 76 54 BanII G_rGCy'C Cuts at: 1391 2118 2486 3639 4806 4968 5472 6145 6728 Size: 727 368 1153 1167 162 504 673 583 Cuts at: 6728 1391 Size: 4072 Fragments arranged by size: 4072 1167 1153 727 673 583 504 368 162 BbeI G_GCGC'C Cuts at: 2030 2288 2403 2671 2030 Size: 258 115 268 8768 Fragments arranged by size: 8768 268 258 115 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3929 3946 3979 5568 6311 928 Size: 971 2030 17 33 1589 743 4026 Fragments arranged by size: 4026 2030 1589 971 743 33 17 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5359 5393 7600 7634 1903 Size: 34 3422 34 2207 34 3678 Fragments arranged by size: 3678 3422 2207 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3860 4217 7301 8511 3860 Size: 357 3084 1210 4758 Fragments arranged by size: 4758 3084 1210 357 BclI T'GATC_A Cuts at: 1548 1548 Size: 9409 BfrBI ATG'CAT Cuts at: 5128 6359 5128 Size: 1231 8178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 2153 8020 Size: 107 122 71 631 605 450 31 5867 Cuts at: 8020 136 Size: 1525 Fragments arranged by size: 5867 1525 631 605 450 122 107 71 31 BglII A'GATC_T Cuts at: 4705 9404 4705 Size: 4699 4710 BlpI GC'TnA_GC Cuts at: 4995 6826 4995 Size: 1831 7578 Bme1580I G_kGCm'C Cuts at: 1790 3449 3524 5795 7470 8626 1790 Size: 1659 75 2271 1675 1156 2573 Fragments arranged by size: 2573 2271 1675 1659 1156 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4431 7140 8098 Size: 416 53 83 399 24 3014 2709 958 Cuts at: 8098 442 Size: 1753 Fragments arranged by size: 3014 2709 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9409 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2293 2983 2293 Size: 690 8719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2878 2967 3207 3528 4820 8069 1239 Size: 1001 638 89 240 321 1292 3249 2579 Fragments arranged by size: 3249 2579 1292 1001 638 321 240 89 Bpu10I CC'TnA_GC Cuts at: 2429 2562 2429 Size: 133 9276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2609 2657 5798 7534 8399 8697 2609 Size: 48 3141 1736 865 298 3321 Fragments arranged by size: 3321 3141 1736 865 298 48 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2017 2281 8072 882 Size: 617 518 264 5791 2219 Fragments arranged by size: 5791 2219 617 518 264 BsaAI yAC'GTr Cuts at: 493 1204 2554 4982 7200 493 Size: 711 1350 2428 2218 2702 Fragments arranged by size: 2702 2428 2218 1350 711 BsaBI GATnn'nnATC Cuts at: 4246 9403 4246 Size: 5157 4252 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2285 2400 2668 Size: 53 83 186 936 494 258 115 268 Cuts at: 2668 2712 2916 5139 6180 6216 7216 7598 8233 Size: 44 204 2223 1041 36 1000 382 635 Cuts at: 8233 275 Size: 1451 Fragments arranged by size: 2223 1451 1041 1000 936 635 494 382 268 258 204 186 115 83 53 44 36 BsaWI w'CCGG_w Cuts at: 3663 4968 5584 7842 8240 8514 8661 9072 3663 Size: 1305 616 2258 398 274 147 411 4000 Fragments arranged by size: 4000 2258 1305 616 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3340 3370 3450 3480 5813 Size: 30 165 30 1937 30 80 30 2333 Cuts at: 5813 5843 1178 Size: 30 4744 Fragments arranged by size: 4744 2333 1937 165 80 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2876 2881 3361 3948 5402 1186 Size: 1690 5 480 587 1454 5193 Fragments arranged by size: 5193 1690 1454 587 480 5 BseYI C'CCAG_C Cuts at: 2662 2789 3036 3332 3799 4789 8612 9108 2662 Size: 127 247 296 467 990 3823 496 2963 Fragments arranged by size: 3823 2963 990 496 467 296 247 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2248 2684 3655 5664 1163 Size: 573 512 436 971 2009 4908 Fragments arranged by size: 4908 2009 971 573 512 436 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 2129 2355 2395 2493 2875 Size: 275 186 100 28 226 40 98 382 Cuts at: 2875 2943 3054 4890 6859 7620 8648 1540 Size: 68 111 1836 1969 761 1028 2301 Fragments arranged by size: 2301 1969 1836 1028 761 382 275 226 186 111 100 98 68 40 28 BsiHKAI G_wGCw'C Cuts at: 2226 2816 3449 3639 4806 4857 4968 6145 7470 Size: 590 633 190 1167 51 111 1177 1325 Cuts at: 7470 7555 8626 2226 Size: 85 1071 3009 Fragments arranged by size: 3009 1325 1177 1167 1071 633 590 190 111 85 51 BsmI GAATG_Cn' Cuts at: 3800 5723 5990 6347 6920 7013 3800 Size: 1923 267 357 573 93 6196 Fragments arranged by size: 6196 1923 573 357 267 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2564 5130 5377 2564 Size: 2566 247 6596 Fragments arranged by size: 6596 2566 247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2226 2486 2816 3449 3524 3639 Size: 399 328 108 260 330 633 75 115 Cuts at: 3639 4806 4857 4968 5472 5795 6145 6728 7470 Size: 1167 51 111 504 323 350 583 742 Cuts at: 7470 7555 8626 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1167 1071 742 633 583 504 399 350 330 328 323 260 115 111 108 85 75 51 BspEI T'CCGG_A Cuts at: 4968 5584 9072 4968 Size: 616 3488 5305 Fragments arranged by size: 5305 3488 616 BspHI T'CATG_A Cuts at: 7298 9028 7298 Size: 1730 7679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3677 5569 6764 844 Size: 2833 1892 1195 3489 Fragments arranged by size: 3489 2833 1892 1195 BsrBI CCG'CTC Cuts at: 1394 1823 2187 2769 2962 7296 1394 Size: 429 364 582 193 4334 3507 Fragments arranged by size: 4334 3507 582 429 364 193 BsrDI GCAATG_nn' Cuts at: 65 1248 4254 4755 5280 7904 8078 65 Size: 1183 3006 501 525 2624 174 1396 Fragments arranged by size: 3006 2624 1396 1183 525 501 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2272 2354 2378 2492 2780 8053 8240 Size: 110 462 82 24 114 288 5273 187 Cuts at: 8240 1700 Size: 2869 Fragments arranged by size: 5273 2869 462 288 187 114 110 82 24 BsrGI T'GTAC_A Cuts at: 96 3466 5547 96 Size: 3370 2081 3958 Fragments arranged by size: 3958 3370 2081 BssHII G'CGCG_C Cuts at: 1925 2798 3017 3019 1925 Size: 873 219 2 8315 Fragments arranged by size: 8315 873 219 2 BssSI C'ACGA_G Cuts at: 7469 8481 7469 Size: 1012 8397 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9409 BstBI TT'CG_AA Cuts at: 3541 8213 3541 Size: 4672 4737 BstEII G'GTnAC_C Cuts at: 1184 4897 1184 Size: 3713 5696 BstXI CCAn_nnnn'nTGG Cuts at: 2902 4217 2902 Size: 1315 8094 BstYI r'GATC_y Cuts at: 1972 3009 4241 4705 5894 6208 6739 7106 7491 Size: 1037 1232 464 1189 314 531 367 385 Cuts at: 7491 7508 8960 9046 9058 9404 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1232 1189 1037 531 464 385 367 346 314 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2592 2857 2979 3150 4651 4890 Size: 552 939 588 265 122 171 1501 239 Cuts at: 4890 5520 513 Size: 630 4402 Fragments arranged by size: 4402 1501 939 630 588 552 265 239 171 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2134 2602 2788 487 Size: 683 56 908 468 186 7108 Fragments arranged by size: 7108 908 683 468 186 56 BtsI GCAGTG_nn' Cuts at: 2057 3061 3415 5285 5959 6925 7719 7739 2057 Size: 1004 354 1870 674 966 794 20 3727 Fragments arranged by size: 3727 1870 1004 966 794 674 354 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9374 DraI TTT'AAA Cuts at: 4443 6713 4443 Size: 2270 7139 DraIII CAC_nnn'GTG Cuts at: 2707 2707 Size: 9409 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8416 809 Size: 507 7100 1802 Fragments arranged by size: 7100 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2352 2490 Size: 54 1425 50 275 18 268 254 138 Cuts at: 2490 2541 2613 2649 2854 2860 2872 2940 3051 Size: 51 72 36 205 6 12 68 111 Cuts at: 3051 3193 4887 5695 5872 6856 7745 9374 8 Size: 142 1694 808 177 984 889 1629 43 Fragments arranged by size: 1694 1629 1425 984 889 808 275 268 254 205 177 142 138 111 72 68 54 51 50 43 36 18 12 6 EagI C'GGCC_G Cuts at: 1537 1812 2098 2352 2490 2872 2940 3051 4887 Size: 275 286 254 138 382 68 111 1836 Cuts at: 4887 6856 1537 Size: 1969 4090 Fragments arranged by size: 4090 1969 1836 382 286 275 254 138 111 68 EarI CTCTTCn'nnn_ Cuts at: 1353 2999 3261 4176 4237 4652 4771 6255 7339 Size: 1646 262 915 61 415 119 1484 1084 Cuts at: 7339 1353 Size: 3423 Fragments arranged by size: 3423 1646 1484 1084 915 415 262 119 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7996 8370 8516 387 Size: 1064 138 6407 374 146 1280 Fragments arranged by size: 6407 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3637 4804 4966 6143 3637 Size: 1167 162 1177 6903 Fragments arranged by size: 6903 1177 1167 162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2302 2878 2967 2980 3207 3528 Size: 393 608 62 576 89 13 227 321 Cuts at: 3528 4820 5139 5741 5919 6293 7472 8069 8856 Size: 1292 319 602 178 374 1179 597 787 Cuts at: 8856 9124 1239 Size: 268 1524 Fragments arranged by size: 1524 1292 1179 787 608 602 597 576 393 374 321 319 268 227 178 89 62 13 EcoNI CCTnn'n_nnAGG Cuts at: 2903 5091 2903 Size: 2188 7221 EcoO109I rG'GnC_Cy Cuts at: 2908 4531 5516 2908 Size: 1623 985 6801 Fragments arranged by size: 6801 1623 985 EcoRI G'AATT_C Cuts at: 3351 5588 6718 7096 3351 Size: 2237 1130 378 5664 Fragments arranged by size: 5664 2237 1130 378 EcoRV GAT'ATC Cuts at: 5886 6427 5886 Size: 541 8868 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3465 3465 Size: 9409 FseI GG_CCGG'CC Cuts at: 2358 2358 Size: 9409 FspI TGC'GCA Cuts at: 2447 4460 7915 2447 Size: 2013 3455 3941 Fragments arranged by size: 3941 3455 2013 FspAI rTGC'GCAy Cuts at: 4460 4460 Size: 9409 HaeII r_GCGC'y Cuts at: 1678 2030 2057 2288 2403 2671 2775 2968 2987 Size: 352 27 231 115 268 104 193 19 Cuts at: 2987 8556 1678 Size: 5569 2531 Fragments arranged by size: 5569 2531 352 268 231 193 115 104 27 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 3119 3151 3488 Size: 32 135 32 878 32 637 32 337 Cuts at: 3488 3520 3911 3943 4805 4837 5813 5845 8050 Size: 32 391 32 862 32 976 32 2205 Cuts at: 8050 8082 8124 8156 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2205 976 878 862 637 391 337 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3721 4940 6072 6753 6999 669 Size: 988 281 1783 1219 1132 681 246 3079 Fragments arranged by size: 3079 1783 1219 1132 988 681 281 246 HindIII A'AGCT_T Cuts at: 748 3580 5194 6407 748 Size: 2832 1614 1213 3750 Fragments arranged by size: 3750 2832 1614 1213 HpaI GTT'AAC Cuts at: 6999 6999 Size: 9409 KasI G'GCGC_C Cuts at: 2026 2284 2399 2667 2026 Size: 258 115 268 8768 Fragments arranged by size: 8768 268 258 115 KpnI G_GTAC'C Cuts at: 2533 6734 2533 Size: 4201 5208 MfeI C'AATT_G Cuts at: 7008 7008 Size: 9409 MluI A'CGCG_T Cuts at: 9285 9285 Size: 9409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2109 2625 3118 3150 5443 5636 8523 8707 Size: 804 516 493 32 2293 193 2887 184 Cuts at: 8707 1305 Size: 2007 Fragments arranged by size: 2887 2293 2007 804 516 493 193 184 32 MscI TGG'CCA Cuts at: 10 64 1489 1832 2651 3195 5697 5874 10 Size: 54 1425 343 819 544 2502 177 3545 Fragments arranged by size: 3545 2502 1425 819 544 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2552 2819 4085 4157 6540 7367 Size: 648 1293 93 267 1266 72 2383 827 Cuts at: 7367 7726 7885 518 Size: 359 159 2042 Fragments arranged by size: 2383 2042 1293 1266 827 648 359 267 159 93 72 MspA1I CmG'CkG Cuts at: 1606 2193 2206 2526 2583 2594 2945 3036 3332 Size: 587 13 320 57 11 351 91 296 Cuts at: 3332 6825 7502 8650 8895 1606 Size: 3493 677 1148 245 2120 Fragments arranged by size: 3493 2120 1148 677 587 351 320 296 245 91 57 13 11 NaeI GCC'GGC Cuts at: 2356 2494 2356 Size: 138 9271 NarI GG'CG_CC Cuts at: 2027 2285 2400 2668 2027 Size: 258 115 268 8768 Fragments arranged by size: 8768 268 258 115 NcoI C'CATG_G Cuts at: 513 1065 2004 4651 513 Size: 552 939 2647 5271 Fragments arranged by size: 5271 2647 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9409 NgoMIV G'CCGG_C Cuts at: 2354 2492 2354 Size: 138 9271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9409 NotI GC'GGCC_GC Cuts at: 2940 6856 2940 Size: 3916 5493 NsiI A_TGCA'T Cuts at: 5130 6361 5130 Size: 1231 8178 NspI r_CATG'y Cuts at: 1132 1229 3807 5365 6668 6767 8312 1132 Size: 97 2578 1558 1303 99 1545 2229 Fragments arranged by size: 2578 2229 1558 1545 1303 99 97 PasI CC'CwG_GG Cuts at: 1348 2419 1348 Size: 1071 8338 PciI A'CATG_T Cuts at: 8308 8308 Size: 9409 PflMI CCAn_nnn'nTGG Cuts at: 1108 2784 1108 Size: 1676 7733 PfoI T'CCnGG_A Cuts at: 2015 5451 5628 2015 Size: 3436 177 5796 Fragments arranged by size: 5796 3436 177 PmeI GTTT'AAAC Cuts at: 6713 6713 Size: 9409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2810 2842 7478 7510 8991 9023 2810 Size: 32 4636 32 1481 32 3196 Fragments arranged by size: 4636 3196 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4531 5516 4531 Size: 985 8424 PshAI GACnn'nnGTC Cuts at: 1613 5256 1613 Size: 3643 5766 PsiI TTA'TAA Cuts at: 6979 6979 Size: 9409 PstI C_TGCA'G Cuts at: 830 4521 5004 6761 830 Size: 3691 483 1757 3478 Fragments arranged by size: 3691 3478 1757 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9409 PvuII CAG'CTG Cuts at: 2193 2206 3036 3332 2193 Size: 13 830 296 8270 Fragments arranged by size: 8270 830 296 13 SacI G_AGCT'C Cuts at: 3639 4806 4968 6145 3639 Size: 1167 162 1177 6903 Fragments arranged by size: 6903 1177 1167 162 SacII CC_GC'GG Cuts at: 2595 2595 Size: 9409 SalI G'TCGA_C Cuts at: 1936 6070 6751 1936 Size: 4134 681 4594 Fragments arranged by size: 4594 4134 681 SanDI GG'GwC_CC Cuts at: 5516 5516 Size: 9409 SapI GCTCTTCn'nnn_ Cuts at: 4237 4237 Size: 9409 SbfI CC_TGCA'GG Cuts at: 6761 6761 Size: 9409 ScaI AGT'ACT Cuts at: 5596 5948 7657 9369 5596 Size: 352 1709 1712 5636 Fragments arranged by size: 5636 1712 1709 352 SfcI C'TryA_G Cuts at: 826 1045 2212 3561 3676 4517 5000 6757 7892 Size: 219 1167 1349 115 841 483 1757 1135 Cuts at: 7892 8573 8764 826 Size: 681 191 1471 Fragments arranged by size: 1757 1471 1349 1167 1135 841 681 483 219 191 115 SfoI GGC'GCC Cuts at: 2028 2286 2401 2669 2028 Size: 258 115 268 8768 Fragments arranged by size: 8768 268 258 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9409 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9409 SmaI CCC'GGG Cuts at: 1872 2308 4070 6736 1872 Size: 436 1762 2666 4545 Fragments arranged by size: 4545 2666 1762 436 SmlI C'TyrA_G Cuts at: 820 1017 1943 2624 2672 3203 5728 5777 7513 Size: 197 926 681 48 531 2525 49 1736 Cuts at: 7513 8414 8676 820 Size: 901 262 1553 Fragments arranged by size: 2525 1736 1553 926 901 681 531 262 197 49 48 SnaBI TAC'GTA Cuts at: 493 7200 493 Size: 6707 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9409 SphI G_CATG'C Cuts at: 1132 3807 6767 1132 Size: 2675 2960 3774 Fragments arranged by size: 3774 2960 2675 SspI AAT'ATT Cuts at: 5 52 4403 4618 4690 5662 6596 7333 5 Size: 47 4351 215 72 972 934 737 2081 Fragments arranged by size: 4351 2081 972 934 737 215 72 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9409 StyI C'CwwG_G Cuts at: 513 1065 2004 3945 4651 6075 6420 6848 513 Size: 552 939 1941 706 1424 345 428 3074 Fragments arranged by size: 3074 1941 1424 939 706 552 428 345 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 5120 7436 7453 7606 7791 8236 Size: 1255 2589 951 2316 17 153 185 445 Cuts at: 8236 325 Size: 1498 Fragments arranged by size: 2589 2316 1498 1255 951 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3466 4486 5547 Size: 274 80 33 51 1443 1489 1020 1061 Cuts at: 5547 5594 5946 7655 9367 96 Size: 47 352 1709 1712 138 Fragments arranged by size: 1712 1709 1489 1443 1061 1020 352 274 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3340 3372 4291 4323 9369 9401 3340 Size: 32 919 32 5046 32 3348 Fragments arranged by size: 5046 3348 919 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9409 XbaI T'CTAG_A Cuts at: 6745 6745 Size: 9409 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2659 4217 4658 5341 2659 Size: 1558 441 683 6727 Fragments arranged by size: 6727 1558 683 441 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9409 XmaI C'CCGG_G Cuts at: 1870 2306 4068 6734 1870 Size: 436 1762 2666 4545 Fragments arranged by size: 4545 2666 1762 436 XmnI GAAnn'nnTTC Cuts at: 7538 7538 Size: 9409 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2917 6217 7217 276 Size: 53 83 186 936 1383 3300 1000 2468 Fragments arranged by size: 3300 2468 1383 1000 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BstZ17I Bsu36I NruI PacI PmlI PspOMI PspXI PsrI RsrII SexAI SfiI SrfI SwaI