Restriction map for
pFN21AE0796
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AocI 670 CCTGAGG CC^T[ACGT]AGG
Aor51HI 716 AGCGCT AGC^GCT
ApaI 1220 GGGCCC GGGCC^C
Asp700I 49 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 193 GGTGCC G^G[CT][GA]CC
BbvCI 1084 GCTGAGG CCTCAGC(-5/-2)
BsgI 997 GTGCAG GTGCAG(16/14)
Bsp1407I 140 TGTACA T^GTACA
BsrDI 495 CATTGC GCAATG(2/0)
BsrGI 140 TGTACA T^GTACA
BstAPI 827 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 670 CCTGAGG CC^T[ACGT]AGG
DpnII 508 GATC ^GATC
DraII 1220 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 716 AGCGCT AGC^GCT
Eco81I 670 CCTGAGG CC^T[ACGT]AGG
EcoNI 1224 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1220 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 991 CCATGG C^C[AT][AT]GG
HindIII 1180 AAGCTT A^AGCTT
MaeII 458 ACGT A^CGT
MboI 508 GATC ^GATC
MseI 1100 TTAA T^TAA
MslI 421 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1082 CAGCTG C[AC]G^C[GT]G
NcoI 991 CCATGG C^CATGG
NdeII 508 GATC ^GATC
PvuII 1082 CAGCTG CAG^CTG
SapI 1074 GCTCTTC GCTCTTC(1/4)
Sau3AI 508 GATC ^GATC
SspBI 140 TGTACA T^GTACA
StyI 991 CCATGG C^C[AT][AT]GG
TfiI 535 GAATC G^A[AT]TC
Tru9I 1100 TTAA T^TAA
XcmI 1230 CCAGGCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 49 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 141 GTAC GT^AC
611 GTAC
BanII 1220 GGGCCC G[GA]GC[CT]^C
1254 GAGCCC
BbsI 1246 GAAGAC GAAGAC(2/6)
685 GTCTTC
BfaI 1135 CTAG C^TAG
1156 CTAG
BpuAI 1246 GAAGAC GAAGAC(2/6)
685 GTCTTC
BseRI 78 GAGGAG GAGGAG(10/8)
1141 GAGGAG
BsmBI 286 GAGACG CGTCTC(1/5)
1215 GAGACG
BstXI 440 CCAGCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
932 CCAAGCTCCTGG
CfoI 717 GCGC GCG^C
968 GCGC
DsaI 991 CCATGG C^C[GA][CT]GG
1239 CCGTGG
EarI 1075 CTCTTC CTCTTC(1/4)
1123 GAAGAG
HaeII 716 AGCGCT [GA]GCGC^[CT]
967 AGCGCC
HgaI 288 GACGC GACGC(5/10)
1071 GACGC
HhaI 717 GCGC GCG^C
968 GCGC
HinP1I 717 GCGC G^CGC
968 GCGC
HphI 70 GGTGA GGTGA(8/7)
1243 GGTGA
Ksp632I 1075 CTCTTC CTCTTC(1/4)
1123 GAAGAG
MaeI 1135 CTAG C^TAG
1156 CTAG
PleI 112 GACTC GAGTC(4/5)
1022 GACTC
RsaI 141 GTAC GT^AC
611 GTAC
SfaNI 949 GATGC GCATC(5/9)
857 GCATC
TaqI 239 TCGA T^CGA
276 TCGA
Tsp509I 158 AATT ^AATT
377 AATT
TthHB8I 239 TCGA T^CGA
276 TCGA
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeIII
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PstI Sau96I
ScrFI SfcI TseI Tsp45I TspRI