(Circular) (Six-base) MAPSORT of: pFN21AB6436.seq Check: 3311 from: 1 to: 5638 pFN21AB6436 5638 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2828 3701 5085 5353 1632 Size: 1196 873 1384 268 1917 Fragments arranged by size: 1917 1384 1196 873 268 AflII C'TTAA_G Cuts at: 820 1017 2288 820 Size: 197 1271 4170 Fragments arranged by size: 4170 1271 197 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 2935 4469 2935 Size: 1534 4104 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2383 2436 1184 Size: 1199 53 4386 Fragments arranged by size: 4386 1199 53 AlwNI CAG_nnn'CTG Cuts at: 3381 4953 3381 Size: 1572 4066 ApaLI G'TGCA_C Cuts at: 2421 3695 4851 2421 Size: 1274 1156 3208 Fragments arranged by size: 3208 1274 1156 ApoI r'AATT_y Cuts at: 1493 2477 2622 2774 2903 2947 3173 3325 5434 Size: 984 145 152 129 44 226 152 2109 Cuts at: 5434 1493 Size: 1697 Fragments arranged by size: 2109 1697 984 226 152 152 145 129 44 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2963 1870 Size: 73 1020 4545 Fragments arranged by size: 4545 1020 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2922 2955 2922 Size: 33 5605 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2756 2959 4414 618 Size: 325 217 627 266 703 203 1455 1842 Fragments arranged by size: 1842 1455 703 627 325 266 217 203 BanII G_rGCy'C Cuts at: 1391 2957 1391 Size: 1566 4072 BbeI G_GCGC'C Cuts at: 2057 2057 Size: 5638 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2848 928 Size: 971 949 3718 Fragments arranged by size: 3718 971 949 BbvCI CC'TCA_GC Cuts at: 2790 2790 Size: 5638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 1548 Size: 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 2051 2062 Size: 107 122 71 631 605 346 33 11 Cuts at: 2062 4249 136 Size: 2187 1525 Fragments arranged by size: 2187 1525 631 605 346 122 107 71 33 11 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 2425 3699 4855 1790 Size: 635 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 635 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2831 2848 3369 Size: 416 53 83 399 24 1414 17 521 Cuts at: 3369 4327 442 Size: 958 1753 Fragments arranged by size: 1753 1414 958 521 416 399 83 53 24 17 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2329 2416 4298 1239 Size: 1028 62 87 1882 2579 Fragments arranged by size: 2579 1882 1028 87 62 Bpu10I CC'TnA_GC Cuts at: 2790 2790 Size: 5638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2594 2709 3763 4628 4926 2594 Size: 115 1054 865 298 3306 Fragments arranged by size: 3306 1054 865 298 115 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 882 Size: 617 2802 2219 Fragments arranged by size: 2802 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 2124 3445 3827 Size: 53 83 186 936 521 70 1321 382 Cuts at: 3827 4462 275 Size: 635 1451 Fragments arranged by size: 1451 1321 936 635 521 382 186 83 70 53 BsaWI w'CCGG_w Cuts at: 2935 4071 4469 4743 4890 5301 2935 Size: 1136 398 274 147 411 3272 Fragments arranged by size: 3272 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2259 2289 2548 2578 1178 Size: 30 165 30 856 30 259 30 4238 Fragments arranged by size: 4238 856 259 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5638 BseYI C'CCAG_C Cuts at: 2035 2087 4841 5337 2035 Size: 52 2754 496 2336 Fragments arranged by size: 2754 2336 496 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5065 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2302 2345 2425 3699 3784 4855 2302 Size: 43 80 1274 85 1071 3085 Fragments arranged by size: 3085 1274 1071 85 80 43 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2302 2345 2425 2957 3699 3784 4855 Size: 399 512 43 80 532 742 85 1071 Cuts at: 4855 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 532 512 399 85 80 43 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4133 4307 65 Size: 1183 2885 174 1396 Fragments arranged by size: 2885 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 2177 2935 4282 4469 1700 Size: 110 246 121 758 1347 187 2869 Fragments arranged by size: 2869 1347 758 246 187 121 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633 Size: 996 367 385 17 1452 86 12 346 Cuts at: 5633 1972 Size: 1977 Fragments arranged by size: 1977 1452 996 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2517 2517 Size: 5638 BtgI C'CryG_G Cuts at: 513 1065 2004 2592 513 Size: 552 939 588 3559 Fragments arranged by size: 3559 939 588 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 3154 3948 3968 3154 Size: 794 20 4824 Fragments arranged by size: 4824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2638 2942 2638 Size: 304 5334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 3085 3974 Size: 54 1425 50 275 18 283 972 889 Cuts at: 3974 5603 8 Size: 1629 43 Fragments arranged by size: 1629 1425 972 889 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 3568 1353 Size: 800 1415 3423 Fragments arranged by size: 3423 1415 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2329 2416 2828 3701 4298 5085 Size: 393 635 62 87 412 873 597 787 Cuts at: 5085 5353 1239 Size: 268 1524 Fragments arranged by size: 1524 873 787 635 597 412 393 268 87 62 EcoO109I rG'GnC_Cy Cuts at: 2330 2330 Size: 5638 EcoRI G'AATT_C Cuts at: 2774 2947 3325 2774 Size: 173 378 5087 Fragments arranged by size: 5087 378 173 FspI TGC'GCA Cuts at: 2072 4144 2072 Size: 2072 3566 HaeII r_GCGC'y Cuts at: 1678 2057 4785 1678 Size: 379 2728 2531 Fragments arranged by size: 2728 2531 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 4279 4311 4353 Size: 32 135 32 638 32 2037 32 42 Cuts at: 4353 4385 1373 Size: 32 2626 Fragments arranged by size: 2626 2037 638 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3228 669 Size: 988 281 1044 246 3079 Fragments arranged by size: 3079 1044 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KasI G'GCGC_C Cuts at: 2053 2053 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3816 Fragments arranged by size: 3816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3596 3955 4114 518 Size: 648 2430 359 159 2042 Fragments arranged by size: 2430 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2136 3054 3731 4879 5124 1606 Size: 501 29 918 677 1148 245 2120 Fragments arranged by size: 2120 1148 918 677 501 245 29 NaeI GCC'GGC Cuts at: 2058 2179 2058 Size: 121 5517 NarI GG'CG_CC Cuts at: 2054 2054 Size: 5638 NcoI C'CATG_G Cuts at: 513 1065 2004 2592 513 Size: 552 939 588 3559 Fragments arranged by size: 3559 939 588 552 NdeI CA'TA_TG Cuts at: 387 2230 387 Size: 1843 3795 NgoMIV G'CCGG_C Cuts at: 2056 2177 2056 Size: 121 5517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2996 4541 1132 Size: 97 1767 1545 2229 Fragments arranged by size: 2229 1767 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5638 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2312 2372 2879 2990 830 Size: 1482 60 507 111 3478 Fragments arranged by size: 3478 1482 507 111 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2308 2368 2875 2986 4121 4802 4993 Size: 219 1263 60 507 111 1135 681 191 Cuts at: 4993 826 Size: 1471 Fragments arranged by size: 1471 1263 1135 681 507 219 191 111 60 SfoI GGC'GCC Cuts at: 2055 2055 Size: 5638 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 2056 1700 Size: 356 5282 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 2573 2724 3742 4643 4905 Size: 197 926 345 285 151 1018 901 262 Cuts at: 4905 820 Size: 1553 Fragments arranged by size: 1553 1018 926 901 345 285 262 197 151 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 2719 2769 3562 5 Size: 47 2667 50 793 2081 Fragments arranged by size: 2667 2081 793 50 47 StuI AGG'CCT Cuts at: 1517 2026 1517 Size: 509 5129 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 3077 513 Size: 552 939 588 485 3074 Fragments arranged by size: 3074 939 588 552 485 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2552 3665 3682 3835 4020 4465 325 Size: 1255 972 1113 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1113 972 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3884 5596 96 Size: 274 80 33 51 1443 1907 1712 138 Fragments arranged by size: 1907 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2259 2291 2548 2580 2695 2727 5598 5630 2259 Size: 32 257 32 115 32 2871 32 2267 Fragments arranged by size: 2871 2267 257 115 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2291 2767 2291 Size: 476 5162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 2667 2718 3767 2667 Size: 51 1049 4538 Fragments arranged by size: 4538 1049 51 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI