(Circular) (Six-base) MAPSORT of: pFN21AB8144.seq Check: 1888 from: 1 to: 5512 pFN21AB8144 5512 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2036 2036 Size: 5512 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3322 278 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2855 1937 Size: 918 4594 Acc65I G'GTAC_C Cuts at: 2833 2833 Size: 5512 AclI AA'CG_TT Cuts at: 1563 3639 4012 1563 Size: 2076 373 3063 Fragments arranged by size: 3063 2076 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 3575 4959 5227 1632 Size: 407 1536 1384 268 1917 Fragments arranged by size: 1917 1536 1384 407 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5315 AflIII A'CryG_T Cuts at: 1203 4411 5388 1203 Size: 3208 977 1327 Fragments arranged by size: 3208 1327 977 AgeI A'CCGG_T Cuts at: 4343 4343 Size: 5512 AhdI GACnn_n'nnGTC Cuts at: 4241 4241 Size: 5512 AleI CACnn'nnGTG Cuts at: 1166 2560 1166 Size: 1394 4118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5512 AlwNI CAG_nnn'CTG Cuts at: 3255 4827 3255 Size: 1572 3940 ApaLI G'TGCA_C Cuts at: 3569 4725 3569 Size: 1156 4356 ApoI r'AATT_y Cuts at: 1493 2265 2663 2821 3047 3199 5308 1493 Size: 772 398 158 226 152 2109 1697 Fragments arranged by size: 2109 1697 772 398 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 4066 160 Size: 872 3034 1606 Fragments arranged by size: 3034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2837 1870 Size: 73 197 697 4545 Fragments arranged by size: 4545 697 197 73 BamHI G'GATC_C Cuts at: 2842 3209 2842 Size: 367 5145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2833 4288 618 Size: 325 217 627 1046 1455 1842 Fragments arranged by size: 1842 1455 1046 627 325 217 BanII G_rGCy'C Cuts at: 1391 2831 1391 Size: 1440 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2362 928 Size: 971 463 4078 Fragments arranged by size: 4078 971 463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2531 2565 3703 3737 1903 Size: 34 50 34 510 34 1138 34 3678 Fragments arranged by size: 3678 1138 510 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3404 4614 3404 Size: 1210 4302 BclI T'GATC_A Cuts at: 1548 1548 Size: 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2115 4123 136 Size: 107 122 71 631 605 443 2008 1525 Fragments arranged by size: 2008 1525 631 605 443 122 107 71 BglII A'GATC_T Cuts at: 2635 5507 2635 Size: 2872 2640 BlpI GC'TnA_GC Cuts at: 2929 2929 Size: 5512 Bme1580I G_kGCm'C Cuts at: 1790 3573 4729 1790 Size: 1783 1156 2573 Fragments arranged by size: 2573 1783 1156 BmgBI CAC'GTC Cuts at: 1318 2049 2562 1318 Size: 731 513 4268 Fragments arranged by size: 4268 731 513 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2169 3243 4201 Size: 416 53 83 399 24 752 1074 958 Cuts at: 4201 442 Size: 1753 Fragments arranged by size: 1753 1074 958 752 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5512 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2298 2298 Size: 5512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 4172 1239 Size: 933 2000 2579 Fragments arranged by size: 2579 2000 933 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3637 4502 4800 3637 Size: 865 298 4349 Fragments arranged by size: 4349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2586 4175 882 Size: 617 1087 1589 2219 Fragments arranged by size: 2219 1589 1087 617 BsaAI yAC'GTr Cuts at: 493 1204 3303 493 Size: 711 2099 2702 Fragments arranged by size: 2702 2099 711 BsaBI GATnn'nnATC Cuts at: 5506 5506 Size: 5512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3319 3701 4336 275 Size: 53 83 186 936 1786 382 635 1451 Fragments arranged by size: 1786 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3945 4343 4617 4764 5175 3945 Size: 398 274 147 411 4282 Fragments arranged by size: 4282 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5287 Fragments arranged by size: 5287 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5512 BseYI C'CCAG_C Cuts at: 2011 4715 5211 2011 Size: 2704 496 2312 Fragments arranged by size: 2704 2312 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2571 1163 Size: 573 835 4104 Fragments arranged by size: 4104 835 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2962 3723 4751 1540 Size: 275 186 961 761 1028 2301 Fragments arranged by size: 2301 1028 961 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3573 3658 4729 3573 Size: 85 1071 4356 Fragments arranged by size: 4356 1071 85 BsmI GAATG_Cn' Cuts at: 3023 3116 3023 Size: 93 5419 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2831 3573 3658 4729 1391 Size: 399 1041 742 85 1071 2174 Fragments arranged by size: 2174 1071 1041 742 399 85 BspEI T'CCGG_A Cuts at: 5175 5175 Size: 5512 BspHI T'CATG_A Cuts at: 3401 5131 3401 Size: 1730 3782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2036 2867 844 Size: 1192 831 3489 Fragments arranged by size: 3489 1192 831 BsrBI CCG'CTC Cuts at: 1394 1823 3399 1394 Size: 429 1576 3507 Fragments arranged by size: 3507 1576 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2098 4007 4181 65 Size: 1183 850 1909 174 1396 Fragments arranged by size: 1909 1396 1183 850 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4156 4343 1700 Size: 110 2346 187 2869 Fragments arranged by size: 2869 2346 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5512 BssSI C'ACGA_G Cuts at: 3572 4584 3572 Size: 1012 4500 BstAPI GCAn_nnn'nTGC Cuts at: 794 2037 794 Size: 1243 4269 BstBI TT'CG_AA Cuts at: 4316 4316 Size: 5512 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5512 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2809 2011 Size: 798 4714 BstYI r'GATC_y Cuts at: 1972 2085 2635 2842 3209 3594 3611 5063 5149 Size: 113 550 207 367 385 17 1452 86 Cuts at: 5149 5161 5507 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 550 385 367 346 207 113 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4960 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4773 Fragments arranged by size: 4773 683 56 BtsI GCAGTG_nn' Cuts at: 2415 3028 3822 3842 2415 Size: 613 794 20 4085 Fragments arranged by size: 4085 794 613 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5512 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5477 DraI TTT'AAA Cuts at: 2368 2816 2368 Size: 448 5064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2050 4519 809 Size: 507 734 2469 1802 Fragments arranged by size: 2469 1802 734 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2959 3848 Size: 54 1425 50 275 18 277 852 889 Cuts at: 3848 5477 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 852 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2959 1537 Size: 275 1147 4090 Fragments arranged by size: 4090 1147 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2540 3442 1353 Size: 1187 902 3423 Fragments arranged by size: 3423 1187 902 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2200 4099 4473 4619 387 Size: 1064 138 611 1899 374 146 1280 Fragments arranged by size: 1899 1280 1064 611 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2172 3575 4172 4959 5227 1239 Size: 393 407 133 1403 597 787 268 1524 Fragments arranged by size: 1524 1403 787 597 407 393 268 133 EcoRI G'AATT_C Cuts at: 2821 3199 2821 Size: 378 5134 FspI TGC'GCA Cuts at: 4018 4018 Size: 5512 HaeII r_GCGC'y Cuts at: 1678 4659 1678 Size: 2981 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2508 2540 2594 2626 4153 Size: 32 135 32 936 32 54 32 1527 Cuts at: 4153 4185 4227 4259 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1527 936 135 54 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2856 3102 669 Size: 988 281 918 246 3079 Fragments arranged by size: 3079 988 918 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5512 HpaI GTT'AAC Cuts at: 3102 3102 Size: 5512 KpnI G_GTAC'C Cuts at: 2837 2837 Size: 5512 MfeI C'AATT_G Cuts at: 3111 3111 Size: 5512 MluI A'CGCG_T Cuts at: 5388 5388 Size: 5512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4626 4810 1305 Size: 3321 184 2007 Fragments arranged by size: 3321 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3690 Fragments arranged by size: 3690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2560 3470 3829 3988 518 Size: 648 1394 910 359 159 2042 Fragments arranged by size: 2042 1394 910 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2127 2169 2928 3605 4753 4998 1606 Size: 405 116 42 759 677 1148 245 2120 Fragments arranged by size: 2120 1148 759 677 405 245 116 42 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4960 NdeI CA'TA_TG Cuts at: 387 2750 387 Size: 2363 3149 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5512 NotI GC'GGCC_GC Cuts at: 2959 2959 Size: 5512 NspI r_CATG'y Cuts at: 1132 1229 2870 4415 1132 Size: 97 1641 1545 2229 Fragments arranged by size: 2229 1641 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5512 PciI A'CATG_T Cuts at: 4411 4411 Size: 5512 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5512 PfoI T'CCnGG_A Cuts at: 2526 2526 Size: 5512 PmeI GTTT'AAAC Cuts at: 2816 2816 Size: 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3581 3613 5094 5126 3581 Size: 32 1481 32 3967 Fragments arranged by size: 3967 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5512 PsiI TTA'TAA Cuts at: 3082 3082 Size: 5512 PstI C_TGCA'G Cuts at: 830 2033 2422 2864 830 Size: 1203 389 442 3478 Fragments arranged by size: 3478 1203 442 389 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5512 SalI G'TCGA_C Cuts at: 1936 2854 1936 Size: 918 4594 SbfI CC_TGCA'GG Cuts at: 2864 2864 Size: 5512 ScaI AGT'ACT Cuts at: 3760 5472 3760 Size: 1712 3800 SfcI C'TryA_G Cuts at: 826 1045 2029 2418 2860 3995 4676 4867 826 Size: 219 984 389 442 1135 681 191 1471 Fragments arranged by size: 1471 1135 984 681 442 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5512 SmaI CCC'GGG Cuts at: 1872 2839 1872 Size: 967 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3616 4517 4779 820 Size: 197 926 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3303 493 Size: 2810 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5512 SphI G_CATG'C Cuts at: 1132 2870 1132 Size: 1738 3774 SspI AAT'ATT Cuts at: 5 52 3436 5 Size: 47 3384 2081 Fragments arranged by size: 3384 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5512 StyI C'CwwG_G Cuts at: 513 1065 2642 2951 513 Size: 552 1577 309 3074 Fragments arranged by size: 3074 1577 552 309 SwaI ATTT'AAAT Cuts at: 2368 2368 Size: 5512 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3539 3556 3709 3894 4339 325 Size: 1255 1959 17 153 185 445 1498 Fragments arranged by size: 1959 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3758 5470 96 Size: 274 80 33 51 1443 1781 1712 138 Fragments arranged by size: 1781 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5472 5504 5472 Size: 32 5480 Tth111I GACn'n_nGTC Cuts at: 1316 2047 1316 Size: 731 4781 XbaI T'CTAG_A Cuts at: 2848 2848 Size: 5512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2102 2102 Size: 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5512 XmaI C'CCGG_G Cuts at: 1870 2837 1870 Size: 967 4545 XmnI GAAnn'nnTTC Cuts at: 2637 3641 2637 Size: 1004 4508 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3320 276 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI