(Circular) (Six-base) MAPSORT of: pFN21AB9481.seq Check: 7789 from: 1 to: 11350 pFN21AB9481 11350 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9160 278 Size: 53 83 186 936 7624 2468 Fragments arranged by size: 7624 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2678 3712 5196 8520 8693 1937 Size: 741 1034 1484 3324 173 4594 Fragments arranged by size: 4594 3324 1484 1034 741 173 Acc65I G'GTAC_C Cuts at: 6918 8671 6918 Size: 1753 9597 AclI AA'CG_TT Cuts at: 1563 3229 8040 9477 9850 1563 Size: 1666 4811 1437 373 3063 Fragments arranged by size: 4811 3063 1666 1437 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2032 2727 3563 4281 4738 4813 5627 5916 Size: 400 695 836 718 457 75 814 289 Cuts at: 5916 7181 7391 7454 7945 9413 10797 11065 1632 Size: 1265 210 63 491 1468 1384 268 1917 Fragments arranged by size: 1917 1468 1384 1265 836 814 718 695 491 457 400 289 268 210 75 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11153 AflIII A'CryG_T Cuts at: 1203 5541 10249 11226 1203 Size: 4338 4708 977 1327 Fragments arranged by size: 4708 4338 1327 977 AgeI A'CCGG_T Cuts at: 10181 10181 Size: 11350 AhdI GACnn_n'nnGTC Cuts at: 4365 8508 10079 4365 Size: 4143 1571 5636 Fragments arranged by size: 5636 4143 1571 AleI CACnn'nnGTG Cuts at: 1166 7023 7127 7886 8360 8370 1166 Size: 5857 104 759 474 10 4146 Fragments arranged by size: 5857 4146 759 474 104 10 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5730 8388 1184 Size: 4546 2658 4146 Fragments arranged by size: 4546 4146 2658 AlwNI CAG_nnn'CTG Cuts at: 4319 4850 5120 5648 5918 6182 6251 8296 9093 Size: 531 270 528 270 264 69 2045 797 Cuts at: 9093 10665 4319 Size: 1572 5004 Fragments arranged by size: 5004 2045 1572 797 531 528 270 270 264 69 ApaI G_GGCC'C Cuts at: 6510 8355 6510 Size: 1845 9505 ApaLI G'TGCA_C Cuts at: 8363 8465 9407 10563 8363 Size: 102 942 1156 9150 Fragments arranged by size: 9150 1156 942 102 ApoI r'AATT_y Cuts at: 1493 2015 2796 3132 4018 4027 4214 4288 4406 Size: 522 781 336 886 9 187 74 118 Cuts at: 4406 5313 5810 7352 7439 8659 8885 9037 11146 Size: 907 497 1542 87 1220 226 152 2109 Cuts at: 11146 1493 Size: 1697 Fragments arranged by size: 2109 1697 1542 1220 907 886 781 522 497 336 226 187 152 118 87 74 9 AseI AT'TA_AT Cuts at: 160 1032 9904 160 Size: 872 8872 1606 Fragments arranged by size: 8872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11350 AvaI C'yCGr_G Cuts at: 1870 1943 8341 8675 1870 Size: 73 6398 334 4545 Fragments arranged by size: 6398 4545 334 73 AvrII C'CTAG_G Cuts at: 7321 7321 Size: 11350 BamHI G'GATC_C Cuts at: 3378 3885 6783 8680 9047 3378 Size: 507 2898 1897 367 5681 Fragments arranged by size: 5681 2898 1897 507 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2272 4234 4657 5955 6918 Size: 325 217 627 485 1962 423 1298 963 Cuts at: 6918 8000 8671 10126 618 Size: 1082 671 1455 1842 Fragments arranged by size: 1962 1842 1455 1298 1082 963 671 627 485 423 325 217 BanII G_rGCy'C Cuts at: 1391 2129 2807 3972 5994 6049 6510 7503 8355 Size: 738 678 1165 2022 55 461 993 852 Cuts at: 8355 8669 1391 Size: 314 4072 Fragments arranged by size: 4072 2022 1165 993 852 738 678 461 314 55 BbeI G_GCGC'C Cuts at: 4661 4661 Size: 11350 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3029 3988 6565 6614 6978 7050 928 Size: 971 1130 959 2577 49 364 72 5228 Fragments arranged by size: 5228 2577 1130 971 959 364 72 49 BbvCI CC'TCA_GC Cuts at: 4637 4637 Size: 11350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2268 2302 2457 2491 9541 9575 1903 Size: 34 331 34 155 34 7050 34 3678 Fragments arranged by size: 7050 3678 331 155 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4918 9242 10452 4918 Size: 4324 1210 5816 Fragments arranged by size: 5816 4324 1210 BclI T'GATC_A Cuts at: 1548 2293 4936 1548 Size: 745 2643 7962 Fragments arranged by size: 7962 2643 745 BfrBI ATG'CAT Cuts at: 5675 5675 Size: 11350 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 9961 136 Size: 107 122 71 631 605 337 7952 1525 Fragments arranged by size: 7952 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5818 6400 11345 5818 Size: 582 4945 5823 Fragments arranged by size: 5823 4945 582 BlpI GC'TnA_GC Cuts at: 2245 5993 8767 2245 Size: 3748 2774 4828 Fragments arranged by size: 4828 3748 2774 Bme1580I G_kGCm'C Cuts at: 1790 2864 6510 6909 8355 8367 8469 9411 10567 Size: 1074 3646 399 1446 12 102 942 1156 Cuts at: 10567 1790 Size: 2573 Fragments arranged by size: 3646 2573 1446 1156 1074 942 399 102 12 BmgBI CAC'GTC Cuts at: 1318 5530 7795 1318 Size: 4212 2265 4873 Fragments arranged by size: 4873 4212 2265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3880 6444 8059 Size: 416 53 83 399 24 2463 2564 1615 Cuts at: 8059 8284 9081 10039 442 Size: 225 797 958 1753 Fragments arranged by size: 2564 2463 1753 1615 958 797 416 399 225 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11350 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7910 8564 7910 Size: 654 10696 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2321 2335 2680 2752 3240 3255 3827 3885 Size: 1082 14 345 72 488 15 572 58 Cuts at: 3885 4055 4447 4947 5576 6020 6259 6887 7217 Size: 170 392 500 629 444 239 628 330 Cuts at: 7217 8405 10010 1239 Size: 1188 1605 2579 Fragments arranged by size: 2579 1605 1188 1082 629 628 572 500 488 444 392 345 330 239 170 72 58 15 14 Bpu10I CC'TnA_GC Cuts at: 4637 4637 Size: 11350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 4481 6033 9475 10340 10638 2330 Size: 2151 1552 3442 865 298 3042 Fragments arranged by size: 3442 3042 2151 1552 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3840 4164 4463 10013 882 Size: 617 2341 324 299 5550 2219 Fragments arranged by size: 5550 2341 2219 617 324 299 BsaAI yAC'GTr Cuts at: 493 1204 6373 6498 9141 493 Size: 711 5169 125 2643 2702 Fragments arranged by size: 5169 2702 2643 711 125 BsaBI GATnn'nnATC Cuts at: 5849 6030 7911 11344 5849 Size: 181 1881 3433 5855 Fragments arranged by size: 5855 3433 1881 181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4658 9157 9539 10174 Size: 53 83 186 936 3125 4499 382 635 Cuts at: 10174 275 Size: 1451 Fragments arranged by size: 4499 3125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3065 4619 7686 9783 10181 10455 10602 11013 3065 Size: 1554 3067 2097 398 274 147 411 3402 Fragments arranged by size: 3402 3067 2097 1554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2457 2487 4593 4623 4857 Size: 30 165 30 1054 30 2106 30 234 Cuts at: 4857 4887 6326 6356 7199 7229 7262 7292 1178 Size: 30 1439 30 843 30 33 30 5236 Fragments arranged by size: 5236 2106 1439 1054 843 234 165 33 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2497 6996 7796 1186 Size: 1311 4499 800 4740 Fragments arranged by size: 4740 4499 1311 800 BseYI C'CCAG_C Cuts at: 5911 6175 8348 10553 11049 5911 Size: 264 2173 2205 496 6212 Fragments arranged by size: 6212 2205 2173 496 264 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2184 2624 6959 1163 Size: 573 448 440 4335 5554 Fragments arranged by size: 5554 4335 573 448 440 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4624 6948 8800 9561 10589 Size: 275 186 1711 912 2324 1852 761 1028 Cuts at: 10589 1540 Size: 2301 Fragments arranged by size: 2324 2301 1852 1711 1028 912 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2489 5153 5541 5994 6049 8367 8469 9411 9496 Size: 2664 388 453 55 2318 102 942 85 Cuts at: 9496 10567 2489 Size: 1071 3272 Fragments arranged by size: 3272 2664 2318 1071 942 453 388 102 85 55 BsmI GAATG_Cn' Cuts at: 4989 5483 6827 7398 7632 8861 8954 4989 Size: 494 1344 571 234 1229 93 7385 Fragments arranged by size: 7385 1344 1229 571 494 234 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4392 5451 4392 Size: 1059 10291 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2489 2807 2864 3972 5153 5541 Size: 399 339 360 318 57 1108 1181 388 Cuts at: 5541 5994 6049 6510 6909 7503 8355 8367 8469 Size: 453 55 461 399 594 852 12 102 Cuts at: 8469 8669 9411 9496 10567 1391 Size: 200 742 85 1071 2174 Fragments arranged by size: 2174 1181 1108 1071 852 742 594 461 453 399 399 388 360 339 318 200 102 85 57 55 12 BspEI T'CCGG_A Cuts at: 3065 11013 3065 Size: 7948 3402 BspHI T'CATG_A Cuts at: 4696 5580 6894 7734 9239 10969 4696 Size: 884 1314 840 1505 1730 5077 Fragments arranged by size: 5077 1730 1505 1314 884 840 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2045 2294 6747 8705 844 Size: 1201 249 4453 1958 3489 Fragments arranged by size: 4453 3489 1958 1201 249 BsrBI CCG'CTC Cuts at: 1394 1823 2244 4840 9237 1394 Size: 429 421 2596 4397 3507 Fragments arranged by size: 4397 3507 2596 429 421 BsrDI GCAATG_nn' Cuts at: 65 1248 4725 5135 6471 6742 6768 7818 9845 Size: 1183 3477 410 1336 271 26 1050 2027 Cuts at: 9845 10019 65 Size: 174 1396 Fragments arranged by size: 3477 2027 1396 1336 1183 1050 410 271 174 26 BsrFI r'CCGG_y Cuts at: 1700 1810 3035 3354 6747 6947 9994 10181 1700 Size: 110 1225 319 3393 200 3047 187 2869 Fragments arranged by size: 3393 3047 2869 1225 319 200 187 110 BsrGI T'GTAC_A Cuts at: 96 5043 5341 6015 6105 96 Size: 4947 298 674 90 5341 Fragments arranged by size: 5341 4947 674 298 90 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11350 BssSI C'ACGA_G Cuts at: 9410 10422 9410 Size: 1012 10338 BstAPI GCAn_nnn'nTGC Cuts at: 794 5672 6550 794 Size: 4878 878 5594 Fragments arranged by size: 5594 4878 878 BstBI TT'CG_AA Cuts at: 10154 10154 Size: 11350 BstEII G'GTnAC_C Cuts at: 1184 7682 8059 1184 Size: 6498 377 4475 Fragments arranged by size: 6498 4475 377 BstXI CCAn_nnnn'nTGG Cuts at: 2359 3335 6865 6964 2359 Size: 976 3530 99 6745 Fragments arranged by size: 6745 3530 976 99 BstYI r'GATC_y Cuts at: 1972 2983 3378 3885 4545 5128 5208 5818 5850 Size: 1011 395 507 660 583 80 610 32 Cuts at: 5850 6400 6635 6704 6783 8629 8680 9047 9432 Size: 550 235 69 79 1846 51 367 385 Cuts at: 9432 9449 10901 10987 10999 11345 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1846 1452 1011 660 610 583 550 507 395 385 367 346 235 86 80 79 69 51 32 17 12 Bsu36I CC'TnA_GG Cuts at: 5930 5930 Size: 11350 BtgI C'CryG_G Cuts at: 513 1065 2004 2230 3328 3525 4518 6762 7600 Size: 552 939 226 1098 197 993 2244 838 Cuts at: 7600 8269 513 Size: 669 3594 Fragments arranged by size: 3594 2244 1098 993 939 838 669 552 226 197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10611 Fragments arranged by size: 10611 683 56 BtsI GCAGTG_nn' Cuts at: 2162 2842 3177 3777 6551 7156 7380 8365 8377 Size: 680 335 600 2774 605 224 985 12 Cuts at: 8377 8469 8866 9660 9680 2162 Size: 92 397 794 20 3832 Fragments arranged by size: 3832 2774 985 794 680 605 600 397 335 224 92 20 12 ClaI AT'CG_AT Cuts at: 1554 5278 7852 1554 Size: 3724 2574 5052 Fragments arranged by size: 5052 3724 2574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6751 6786 529 Size: 35 6187 35 5093 Fragments arranged by size: 6187 5093 35 35 DraI TTT'AAA Cuts at: 3694 8654 3694 Size: 4960 6390 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6062 8273 10357 809 Size: 507 4746 2211 2084 1802 Fragments arranged by size: 4746 2211 2084 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 4344 6489 Size: 54 1425 50 275 18 381 2133 2145 Cuts at: 6489 6765 6945 8105 8261 8797 9686 11315 8 Size: 276 180 1160 156 536 889 1629 43 Fragments arranged by size: 2145 2133 1629 1425 1160 889 536 381 276 275 180 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6945 8797 1537 Size: 275 5133 1852 4090 Fragments arranged by size: 5133 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2230 2703 2952 3899 4300 4555 5225 7960 Size: 877 473 249 947 401 255 670 2735 Cuts at: 7960 9280 1353 Size: 1320 3423 Fragments arranged by size: 3423 2735 1320 947 877 670 473 401 255 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2738 2768 5647 8228 9937 10311 Size: 1064 138 1149 30 2879 2581 1709 374 Cuts at: 10311 10457 387 Size: 146 1280 Fragments arranged by size: 2879 2581 1709 1280 1149 1064 374 146 138 30 EcoICRI GAG'CTC Cuts at: 5992 6047 5992 Size: 55 11295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2321 2335 2680 2727 2752 3240 Size: 393 400 289 14 345 47 25 488 Cuts at: 3240 3255 3563 3827 3885 4055 4281 4447 4738 Size: 15 308 264 58 170 226 166 291 Cuts at: 4738 4813 4947 5576 5627 5916 6020 6259 6887 Size: 75 134 629 51 289 104 239 628 Cuts at: 6887 7181 7217 7391 7454 7945 8405 9413 10010 Size: 294 36 174 63 491 460 1008 597 Cuts at: 10010 10797 11065 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1008 787 629 628 597 491 488 460 400 393 345 308 294 291 289 289 268 264 239 226 174 170 166 134 104 75 63 58 51 47 36 25 15 14 EcoO109I rG'GnC_Cy Cuts at: 5601 6325 6506 6507 7429 8137 5601 Size: 724 181 1 922 708 8814 Fragments arranged by size: 8814 922 724 708 181 1 EcoRI G'AATT_C Cuts at: 4027 4214 7439 8659 9037 4027 Size: 187 3225 1220 378 6340 Fragments arranged by size: 6340 3225 1220 378 187 EcoRV GAT'ATC Cuts at: 5740 5740 Size: 11350 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2324 5548 8029 2324 Size: 3224 2481 5645 Fragments arranged by size: 5645 3224 2481 FspI TGC'GCA Cuts at: 5141 5774 9856 5141 Size: 633 4082 6635 Fragments arranged by size: 6635 4082 633 HaeII r_GCGC'y Cuts at: 1678 4661 10497 1678 Size: 2983 5836 2531 Fragments arranged by size: 5836 2983 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2346 2378 2614 2646 2932 Size: 32 135 32 774 32 236 32 286 Cuts at: 2932 2964 3820 3852 4351 4383 5724 5756 6109 Size: 32 856 32 499 32 1341 32 353 Cuts at: 6109 6141 6625 6657 7519 7551 7588 7620 7904 Size: 32 484 32 862 32 37 32 284 Cuts at: 7904 7936 8494 8526 8619 8651 9991 10023 10065 Size: 32 558 32 93 32 1340 32 42 Cuts at: 10065 10097 1373 Size: 32 2626 Fragments arranged by size: 2626 1341 1340 862 856 774 558 499 484 353 286 284 236 135 93 42 37 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2410 2679 3713 5197 5506 6055 Size: 988 281 472 269 1034 1484 309 549 Cuts at: 6055 7078 8694 8940 669 Size: 1023 1616 246 3079 Fragments arranged by size: 3079 1616 1484 1034 1023 988 549 472 309 281 269 246 HindIII A'AGCT_T Cuts at: 748 7298 748 Size: 6550 4800 HpaI GTT'AAC Cuts at: 8940 8940 Size: 11350 KasI G'GCGC_C Cuts at: 4657 4657 Size: 11350 KpnI G_GTAC'C Cuts at: 6922 8675 6922 Size: 1753 9597 MfeI C'AATT_G Cuts at: 8949 8949 Size: 11350 MluI A'CGCG_T Cuts at: 11226 11226 Size: 11350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 7779 10464 10648 1305 Size: 939 5535 2685 184 2007 Fragments arranged by size: 5535 2685 2007 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 4346 6491 8107 8263 Size: 54 1425 343 381 2133 2145 1616 156 Cuts at: 8263 10 Size: 3097 Fragments arranged by size: 3097 2145 2133 1616 1425 381 343 156 54 MslI CAynn'nnrTG Cuts at: 518 1166 2278 2893 3393 3467 3926 4092 4434 Size: 648 1112 615 500 74 459 166 342 Cuts at: 4434 6896 6935 7023 7025 7127 7223 7556 7886 Size: 2462 39 88 2 102 96 333 330 Cuts at: 7886 8054 8360 8370 8532 9308 9667 9826 518 Size: 168 306 10 162 776 359 159 2042 Fragments arranged by size: 2462 2042 1112 776 648 615 500 459 359 342 333 330 306 168 166 162 159 102 96 88 74 39 10 2 MspA1I CmG'CkG Cuts at: 1606 2232 2262 3212 3735 5215 5598 5918 5970 Size: 626 30 950 523 1480 383 320 52 Cuts at: 5970 5997 6422 7086 8111 8766 9443 10591 10836 Size: 27 425 664 1025 655 677 1148 245 Cuts at: 10836 1606 Size: 2120 Fragments arranged by size: 2120 1480 1148 1025 950 677 664 655 626 523 425 383 320 245 52 30 27 NaeI GCC'GGC Cuts at: 6749 6949 6749 Size: 200 11150 NarI GG'CG_CC Cuts at: 4658 4658 Size: 11350 NcoI C'CATG_G Cuts at: 513 1065 2004 3328 3525 7600 8269 513 Size: 552 939 1324 197 4075 669 3594 Fragments arranged by size: 4075 3594 1324 939 669 552 197 NdeI CA'TA_TG Cuts at: 387 3082 387 Size: 2695 8655 NgoMIV G'CCGG_C Cuts at: 6747 6947 6747 Size: 200 11150 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11350 NotI GC'GGCC_GC Cuts at: 8797 8797 Size: 11350 NsiI A_TGCA'T Cuts at: 5677 5677 Size: 11350 NspI r_CATG'y Cuts at: 1132 1229 3047 3413 4091 4343 4922 5168 7555 Size: 97 1818 366 678 252 579 246 2387 Cuts at: 7555 8651 8708 10253 1132 Size: 1096 57 1545 2229 Fragments arranged by size: 2387 2229 1818 1545 1096 678 579 366 252 246 97 57 PasI CC'CwG_GG Cuts at: 1348 2845 1348 Size: 1497 9853 PciI A'CATG_T Cuts at: 10249 10249 Size: 11350 PflMI CCAn_nnn'nTGG Cuts at: 1108 5554 6251 6498 6701 8270 1108 Size: 4446 697 247 203 1569 4188 Fragments arranged by size: 4446 4188 1569 697 247 203 PfoI T'CCnGG_A Cuts at: 3598 6036 6358 6674 8187 3598 Size: 2438 322 316 1513 6761 Fragments arranged by size: 6761 2438 1513 322 316 PmeI GTTT'AAAC Cuts at: 8654 8654 Size: 11350 PmlI CAC'GTG Cuts at: 6498 6498 Size: 11350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5528 5560 9419 9451 10932 10964 5528 Size: 32 3859 32 1481 32 5914 Fragments arranged by size: 5914 3859 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5601 6325 7429 8137 5601 Size: 724 1104 708 8814 Fragments arranged by size: 8814 1104 724 708 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11350 PsiI TTA'TAA Cuts at: 8920 8920 Size: 11350 PspOMI G'GGCC_C Cuts at: 6506 8351 6506 Size: 1845 9505 PstI C_TGCA'G Cuts at: 830 2596 3484 6546 7085 7586 8151 8372 8702 Size: 1766 888 3062 539 501 565 221 330 Cuts at: 8702 830 Size: 3478 Fragments arranged by size: 3478 3062 1766 888 565 539 501 330 221 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11350 PvuII CAG'CTG Cuts at: 3212 3735 5215 5918 5997 7086 3212 Size: 523 1480 703 79 1089 7476 Fragments arranged by size: 7476 1480 1089 703 523 79 SacI G_AGCT'C Cuts at: 5994 6049 5994 Size: 55 11295 SacII CC_GC'GG Cuts at: 2233 2233 Size: 11350 SalI G'TCGA_C Cuts at: 1936 2677 3711 5195 8692 1936 Size: 741 1034 1484 3497 4594 Fragments arranged by size: 4594 3497 1484 1034 741 SanDI GG'GwC_CC Cuts at: 5601 5601 Size: 11350 SapI GCTCTTCn'nnn_ Cuts at: 5225 5225 Size: 11350 SbfI CC_TGCA'GG Cuts at: 8702 8702 Size: 11350 ScaI AGT'ACT Cuts at: 3145 9598 11310 3145 Size: 6453 1712 3185 Fragments arranged by size: 6453 3185 1712 SexAI A'CCwGG_T Cuts at: 4230 6921 4230 Size: 2691 8659 SfcI C'TryA_G Cuts at: 826 1045 2592 2602 2830 3480 3496 4128 4257 Size: 219 1547 10 228 650 16 632 129 Cuts at: 4257 6542 7081 7387 7582 7742 8147 8368 8698 Size: 2285 539 306 195 160 405 221 330 Cuts at: 8698 9833 10514 10705 826 Size: 1135 681 191 1471 Fragments arranged by size: 2285 1547 1471 1135 681 650 632 539 405 330 306 228 221 219 195 191 160 129 16 10 SfoI GGC'GCC Cuts at: 4659 4659 Size: 11350 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11350 SmaI CCC'GGG Cuts at: 1872 8343 8677 1872 Size: 6471 334 4545 Fragments arranged by size: 6471 4545 334 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 4496 6048 9454 10355 10617 Size: 197 926 366 2187 1552 3406 901 262 Cuts at: 10617 820 Size: 1553 Fragments arranged by size: 3406 2187 1553 1552 926 901 366 262 197 SnaBI TAC'GTA Cuts at: 493 9141 493 Size: 8648 2702 SpeI A'CTAG_T Cuts at: 152 3508 6061 152 Size: 3356 2553 5441 Fragments arranged by size: 5441 3356 2553 SphI G_CATG'C Cuts at: 1132 4922 7555 8708 1132 Size: 3790 2633 1153 3774 Fragments arranged by size: 3790 3774 2633 1153 SspI AAT'ATT Cuts at: 5 52 3199 5395 9274 5 Size: 47 3147 2196 3879 2081 Fragments arranged by size: 3879 3147 2196 2081 47 StuI AGG'CCT Cuts at: 1517 2576 2765 5871 6351 1517 Size: 1059 189 3106 480 6516 Fragments arranged by size: 6516 3106 1059 480 189 StyI C'CwwG_G Cuts at: 513 1065 2004 2760 3328 3525 3671 4042 4351 Size: 552 939 756 568 197 146 371 309 Cuts at: 4351 5653 6486 7321 7600 7770 8004 8269 8789 Size: 1302 833 835 279 170 234 265 520 Cuts at: 8789 513 Size: 3074 Fragments arranged by size: 3074 1302 939 835 833 756 568 552 520 371 309 279 265 234 197 170 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4903 6370 6854 8303 8427 9377 9394 Size: 1255 3323 1467 484 1449 124 950 17 Cuts at: 9394 9547 9732 10177 325 Size: 153 185 445 1498 Fragments arranged by size: 3323 1498 1467 1449 1255 950 484 445 185 153 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3143 4512 5043 Size: 274 80 33 51 1443 1166 1369 531 Cuts at: 5043 5341 6015 6105 7200 9596 11308 96 Size: 298 674 90 1095 2396 1712 138 Fragments arranged by size: 2396 1712 1443 1369 1166 1095 674 531 298 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3246 3278 4299 4331 4593 4625 5434 5466 6597 Size: 32 1021 32 262 32 809 32 1131 Cuts at: 6597 6629 7199 7231 11310 11342 3246 Size: 32 570 32 4079 32 3254 Fragments arranged by size: 4079 3254 1131 1021 809 570 262 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4856 5173 1316 Size: 3540 317 7493 Fragments arranged by size: 7493 3540 317 XbaI T'CTAG_A Cuts at: 8686 8686 Size: 11350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 8443 8443 Size: 11350 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11350 XmaI C'CCGG_G Cuts at: 1870 8341 8675 1870 Size: 6471 334 4545 Fragments arranged by size: 6471 4545 334 XmnI GAAnn'nnTTC Cuts at: 2064 3229 7118 7439 9479 2064 Size: 1165 3889 321 2040 3935 Fragments arranged by size: 3935 3889 2040 1165 321 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9158 276 Size: 53 83 186 936 7624 2468 Fragments arranged by size: 7624 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BsiWI BstZ17I DraIII EcoNI FseI FspAI NruI PacI PspXI PsrI RsrII SfiI SrfI SwaI