(Circular) (Six-base) MAPSORT of: pFN21AE0033.seq Check: 9979 from: 1 to: 6397 pFN21AE0033 6397 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3655 3655 Size: 6397 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4207 278 Size: 53 83 186 936 2671 2468 Fragments arranged by size: 2671 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3740 1937 Size: 1803 4594 Acc65I G'GTAC_C Cuts at: 3718 3718 Size: 6397 AclI AA'CG_TT Cuts at: 1563 4524 4897 1563 Size: 2961 373 3063 Fragments arranged by size: 3063 2961 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 4460 5844 6112 1632 Size: 591 2237 1384 268 1917 Fragments arranged by size: 2237 1917 1384 591 268 AfeI AGC'GCT Cuts at: 2059 2059 Size: 6397 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6200 AflIII A'CryG_T Cuts at: 1203 3676 5296 6273 1203 Size: 2473 1620 977 1327 Fragments arranged by size: 2473 1620 1327 977 AgeI A'CCGG_T Cuts at: 5228 5228 Size: 6397 AhdI GACnn_n'nnGTC Cuts at: 5126 5126 Size: 6397 AleI CACnn'nnGTG Cuts at: 1166 2078 1166 Size: 912 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2514 1184 Size: 1330 5067 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3039 3071 3039 Size: 32 6365 AlwNI CAG_nnn'CTG Cuts at: 4140 5712 4140 Size: 1572 4825 ApaI G_GGCC'C Cuts at: 3240 3240 Size: 6397 ApaLI G'TGCA_C Cuts at: 4454 5610 4454 Size: 1156 5241 ApoI r'AATT_y Cuts at: 1493 2415 2744 3137 3428 3706 3932 4084 6193 Size: 922 329 393 291 278 226 152 2109 Cuts at: 6193 1493 Size: 1697 Fragments arranged by size: 2109 1697 922 393 329 291 278 226 152 AseI AT'TA_AT Cuts at: 160 1032 3261 4951 160 Size: 872 2229 1690 1606 Fragments arranged by size: 2229 1690 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6397 AvaI C'yCGr_G Cuts at: 1870 1943 3627 3722 1870 Size: 73 1684 95 4545 Fragments arranged by size: 4545 1684 95 73 BamHI G'GATC_C Cuts at: 3727 4094 3727 Size: 367 6030 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3718 5173 618 Size: 325 217 627 1931 1455 1842 Fragments arranged by size: 1931 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2318 3240 3628 3716 1391 Size: 927 922 388 88 4072 Fragments arranged by size: 4072 927 922 388 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5426 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4588 4622 1903 Size: 34 2651 34 3678 Fragments arranged by size: 3678 2651 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4289 5499 4289 Size: 1210 5187 BclI T'GATC_A Cuts at: 1548 1548 Size: 6397 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 5008 136 Size: 107 122 71 631 605 397 2939 1525 Fragments arranged by size: 2939 1525 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 2646 6392 2646 Size: 3746 2651 BlpI GC'TnA_GC Cuts at: 2054 2976 3814 2054 Size: 922 838 4637 Fragments arranged by size: 4637 922 838 Bme1580I G_kGCm'C Cuts at: 1790 3240 4458 5614 1790 Size: 1450 1218 1156 2573 Fragments arranged by size: 2573 1450 1218 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6397 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2719 3298 4128 Size: 416 53 83 399 24 1302 579 830 Cuts at: 4128 5086 442 Size: 958 1753 Fragments arranged by size: 1753 1302 958 830 579 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6397 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3099 5057 1239 Size: 1860 1958 2579 Fragments arranged by size: 2579 1958 1860 Bpu10I CC'TnA_GC Cuts at: 3600 3600 Size: 6397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4522 5387 5685 4522 Size: 865 298 5234 Fragments arranged by size: 5234 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5060 882 Size: 617 3561 2219 Fragments arranged by size: 3561 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4188 493 Size: 711 2984 2702 Fragments arranged by size: 2984 2702 711 BsaBI GATnn'nnATC Cuts at: 6391 6391 Size: 6397 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4204 4586 5221 275 Size: 53 83 186 936 2671 382 635 1451 Fragments arranged by size: 2671 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4830 5228 5502 5649 6060 4830 Size: 398 274 147 411 5167 Fragments arranged by size: 5167 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2358 2388 3039 3069 3447 Size: 30 165 30 955 30 651 30 378 Cuts at: 3447 3477 1178 Size: 30 4098 Fragments arranged by size: 4098 955 651 378 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3463 1186 Size: 2277 4120 BseYI C'CCAG_C Cuts at: 5600 6096 5600 Size: 496 5901 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3527 1163 Size: 573 1791 4033 Fragments arranged by size: 4033 1791 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3847 4608 5636 1540 Size: 275 186 1846 761 1028 2301 Fragments arranged by size: 2301 1846 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4458 4543 5614 4458 Size: 85 1071 5241 Fragments arranged by size: 5241 1071 85 BsmI GAATG_Cn' Cuts at: 2639 3022 3908 4001 2639 Size: 383 886 93 5035 Fragments arranged by size: 5035 886 383 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2318 3240 3628 3716 4458 4543 5614 Size: 399 528 922 388 88 742 85 1071 Cuts at: 5614 1391 Size: 2174 Fragments arranged by size: 2174 1071 922 742 528 399 388 88 85 BspEI T'CCGG_A Cuts at: 6060 6060 Size: 6397 BspHI T'CATG_A Cuts at: 2728 4286 6016 2728 Size: 1558 1730 3109 Fragments arranged by size: 3109 1730 1558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3655 3752 844 Size: 2811 97 3489 Fragments arranged by size: 3489 2811 97 BsrBI CCG'CTC Cuts at: 1394 1823 4284 1394 Size: 429 2461 3507 Fragments arranged by size: 3507 2461 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3444 4892 5066 65 Size: 1183 2196 1448 174 1396 Fragments arranged by size: 2196 1448 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5041 5228 1700 Size: 110 3231 187 2869 Fragments arranged by size: 3231 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2158 2357 3223 96 Size: 2062 199 866 3270 Fragments arranged by size: 3270 2062 866 199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6397 BssSI C'ACGA_G Cuts at: 4457 5469 4457 Size: 1012 5385 BstAPI GCAn_nnn'nTGC Cuts at: 794 3278 3514 794 Size: 2484 236 3677 Fragments arranged by size: 3677 2484 236 BstBI TT'CG_AA Cuts at: 5201 5201 Size: 6397 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6397 BstXI CCAn_nnnn'nTGG Cuts at: 2080 2080 Size: 6397 BstYI r'GATC_y Cuts at: 1972 2539 2646 3594 3727 4094 4479 4496 5948 Size: 567 107 948 133 367 385 17 1452 Cuts at: 5948 6034 6046 6392 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 948 567 385 367 346 133 107 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4906 Fragments arranged by size: 4906 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5658 Fragments arranged by size: 5658 683 56 BtsI GCAGTG_nn' Cuts at: 2025 3048 3913 4707 4727 2025 Size: 1023 865 794 20 3695 Fragments arranged by size: 3695 1023 865 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6397 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3229 3264 529 Size: 35 2665 35 3662 Fragments arranged by size: 3662 2665 35 35 DraI TTT'AAA Cuts at: 2223 3701 2223 Size: 1478 4919 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3364 5404 809 Size: 507 2048 2040 1802 Fragments arranged by size: 2048 2040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 3844 4733 Size: 54 1425 50 275 18 240 1774 889 Cuts at: 4733 6362 8 Size: 1629 43 Fragments arranged by size: 1774 1629 1425 889 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3844 1537 Size: 275 2032 4090 Fragments arranged by size: 4090 2032 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4327 1353 Size: 2974 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4984 5358 5504 387 Size: 1064 138 3395 374 146 1280 Fragments arranged by size: 3395 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 3099 4460 5057 5844 6112 1239 Size: 393 591 876 1361 597 787 268 1524 Fragments arranged by size: 1524 1361 876 787 597 591 393 268 EcoRI G'AATT_C Cuts at: 2415 3706 4084 2415 Size: 1291 378 4728 Fragments arranged by size: 4728 1291 378 EcoRV GAT'ATC Cuts at: 3129 3129 Size: 6397 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2539 2542 3297 2539 Size: 3 755 5639 Fragments arranged by size: 5639 755 3 FspI TGC'GCA Cuts at: 4903 4903 Size: 6397 HaeII r_GCGC'y Cuts at: 1678 2061 5544 1678 Size: 383 3483 2531 Fragments arranged by size: 3483 2531 383 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5038 5070 5112 5144 1373 Size: 32 135 32 3466 32 42 32 2626 Fragments arranged by size: 3466 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3741 3987 669 Size: 988 281 1803 246 3079 Fragments arranged by size: 3079 1803 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6397 HpaI GTT'AAC Cuts at: 3987 3987 Size: 6397 KpnI G_GTAC'C Cuts at: 3722 3722 Size: 6397 MfeI C'AATT_G Cuts at: 3996 3996 Size: 6397 MluI A'CGCG_T Cuts at: 6273 6273 Size: 6397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2149 3397 5511 5695 1305 Size: 844 1248 2114 184 2007 Fragments arranged by size: 2114 2007 1248 844 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4575 Fragments arranged by size: 4575 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 4355 4714 4873 518 Size: 648 912 2277 359 159 2042 Fragments arranged by size: 2277 2042 912 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2053 3813 4490 5638 5883 1606 Size: 447 1760 677 1148 245 2120 Fragments arranged by size: 2120 1760 1148 677 447 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4906 Fragments arranged by size: 4906 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6397 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6397 NotI GC'GGCC_GC Cuts at: 3844 3844 Size: 6397 NspI r_CATG'y Cuts at: 1132 1229 3680 3755 5300 1132 Size: 97 2451 75 1545 2229 Fragments arranged by size: 2451 2229 1545 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6397 PciI A'CATG_T Cuts at: 3676 5296 3676 Size: 1620 4777 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6397 PmeI GTTT'AAAC Cuts at: 3701 3701 Size: 6397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3039 3071 4466 4498 5979 6011 3039 Size: 32 1395 32 1481 32 3425 Fragments arranged by size: 3425 1481 1395 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6397 PsiI TTA'TAA Cuts at: 3967 3967 Size: 6397 PspOMI G'GGCC_C Cuts at: 3236 3236 Size: 6397 PstI C_TGCA'G Cuts at: 830 2102 3749 830 Size: 1272 1647 3478 Fragments arranged by size: 3478 1647 1272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6397 PvuII CAG'CTG Cuts at: 2053 2053 Size: 6397 SalI G'TCGA_C Cuts at: 1936 3739 1936 Size: 1803 4594 SbfI CC_TGCA'GG Cuts at: 3749 3749 Size: 6397 ScaI AGT'ACT Cuts at: 4645 6357 4645 Size: 1712 4685 SfcI C'TryA_G Cuts at: 826 1045 2098 2758 3745 4880 5561 5752 826 Size: 219 1053 660 987 1135 681 191 1471 Fragments arranged by size: 1471 1135 1053 987 681 660 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6397 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6397 SmaI CCC'GGG Cuts at: 1872 3724 1872 Size: 1852 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4501 5402 5664 820 Size: 197 926 2558 901 262 1553 Fragments arranged by size: 2558 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4188 493 Size: 3695 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6397 SphI G_CATG'C Cuts at: 1132 3755 1132 Size: 2623 3774 SspI AAT'ATT Cuts at: 5 52 4321 5 Size: 47 4269 2081 Fragments arranged by size: 4269 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6397 StyI C'CwwG_G Cuts at: 513 1065 2004 3836 513 Size: 552 939 1832 3074 Fragments arranged by size: 3074 1832 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2730 4424 4441 4594 4779 5224 325 Size: 1255 1150 1694 17 153 185 445 1498 Fragments arranged by size: 1694 1498 1255 1150 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2158 2357 3223 Size: 274 80 33 51 1443 181 199 866 Cuts at: 3223 4643 6355 96 Size: 1420 1712 138 Fragments arranged by size: 1712 1443 1420 866 274 199 181 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2416 2448 2608 2640 6357 6389 2416 Size: 32 160 32 3717 32 2424 Fragments arranged by size: 3717 2424 160 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6397 XbaI T'CTAG_A Cuts at: 2771 3476 3733 2771 Size: 705 257 5435 Fragments arranged by size: 5435 705 257 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6397 XmaI C'CCGG_G Cuts at: 1870 3722 1870 Size: 1852 4545 XmnI GAAnn'nnTTC Cuts at: 2522 3054 4526 2522 Size: 532 1472 4393 Fragments arranged by size: 4393 1472 532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4205 276 Size: 53 83 186 936 2671 2468 Fragments arranged by size: 2671 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI