Restriction map for pFN21AE0025
 (Circular) (Six-base) MAPSORT of: pFN21AE0025.seq  Check: 5072  from: 1  to: 6427



pFN21AE0025  6427 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4237     278 
   Size:         53      83     186     936    2701    2468

  Fragments arranged by size: 

                2701    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3770    1937 
   Size:       1833    4594
 
Acc65I G'GTAC_C

Cuts at:   3748    3748 
   Size:       6427
 
AclI AA'CG_TT

Cuts at:   1563    4554    4927    1563 
   Size:       2991     373    3063

  Fragments arranged by size: 

                3063    2991     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    4490    5874    6142    1632 
   Size:       2858    1384     268    1917

  Fragments arranged by size: 

                2858    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6230
 
AflIII A'CryG_T

Cuts at:   1203    5326    6303    1203 
   Size:       4123     977    1327

  Fragments arranged by size: 

                4123    1327     977
 
AgeI A'CCGG_T

Cuts at:   5258    5258 
   Size:       6427
 
AhdI GACnn_n'nnGTC

Cuts at:   5156    5156 
   Size:       6427
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6427
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6427
 
AlwNI CAG_nnn'CTG

Cuts at:   4170    5742    4170 
   Size:       1572    4855
 
ApaLI G'TGCA_C

Cuts at:   4484    5640    4484 
   Size:       1156    5271
 
ApoI r'AATT_y

Cuts at:   1493    2498    3067    3093    3736    3962    4114    6223    1493 
   Size:       1005     569      26     643     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1005     643     569     226     152      26
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4981     160 
   Size:        872    3949    1606

  Fragments arranged by size: 

                3949    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6427
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2165    2282    2989    3030    3636    3752    1870 
   Size:         73     222     117     707      41     606     116    4545

  Fragments arranged by size: 

                4545     707     606     222     117     116      73      41
            
 
BamHI G'GATC_C

Cuts at:   3757    4124    3757 
   Size:        367    6060
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2169    3748    5203     618 
   Size:        325     217     627     382    1579    1455    1842

  Fragments arranged by size: 

                1842    1579    1455     627     382     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2727    3643    3746    1391 
   Size:       1336     916     103    4072

  Fragments arranged by size: 

                4072    1336     916     103
 
BbeI G_GCGC'C

Cuts at:   2173    2173 
   Size:       6427
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5456
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3491    3525    4618    4652    1903 
   Size:         34    1554      34    1093      34    3678

  Fragments arranged by size: 

                3678    1554    1093      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3326    4319    5529    3326 
   Size:        993    1210    4224

  Fragments arranged by size: 

                4224    1210     993
 
BclI T'GATC_A

Cuts at:   1548    2599    3112    1548 
   Size:       1051     513    4863

  Fragments arranged by size: 

                4863    1051     513
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5038     136 
   Size:        107     122      71     631     605    3366    1525

  Fragments arranged by size: 

                3366    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6422    6422 
   Size:       6427
 
BlpI GC'TnA_GC

Cuts at:   3844    3844 
   Size:       6427
 
Bme1580I G_kGCm'C

Cuts at:   1790    4488    5644    1790 
   Size:       2698    1156    2573

  Fragments arranged by size: 

                2698    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6427
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2182    3379    4158 
   Size:        416      53      83     399      24     765    1197     779

Cuts at:   4158    5116     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1197     958     779     765     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6427
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3662    3662 
   Size:       6427
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2835    5087    1239 
   Size:       1596    2252    2579

  Fragments arranged by size: 

                2579    2252    1596
 
Bpu10I CC'TnA_GC

Cuts at:   3414    3414 
   Size:       6427
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4552    5417    5715    4552 
   Size:        865     298    5264

  Fragments arranged by size: 

                5264     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2165    2896    5090     882 
   Size:        617     666     731    2194    2219

  Fragments arranged by size: 

                2219    2194     731     666     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4218     493 
   Size:        711    3014    2702

  Fragments arranged by size: 

                3014    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6421    6421 
   Size:       6427
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2170    4234    4616    5251 
   Size:         53      83     186     936     637    2064     382     635

Cuts at:   5251     275 
   Size:       1451

  Fragments arranged by size: 

                2064    1451     936     637     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2703    4860    5258    5532    5679    6090    2703 
   Size:       2157     398     274     147     411    3040

  Fragments arranged by size: 

                3040    2157     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2075    2105    2385    2415    2538 
   Size:         30     165      30     672      30     280      30     123

Cuts at:   2538    2568    1178 
   Size:         30    5037

  Fragments arranged by size: 

                5037     672     280     165     123      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2153    3066    1186 
   Size:        967     913    4547

  Fragments arranged by size: 

                4547     967     913
 
BseYI C'CCAG_C

Cuts at:   2116    5630    6126    2116 
   Size:       3514     496    2417

  Fragments arranged by size: 

                3514    2417     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2678    1163 
   Size:        573     942    4912

  Fragments arranged by size: 

                4912     942     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3877    4638    5666    1540 
   Size:        275     186    1876     761    1028    2301

  Fragments arranged by size: 

                2301    1876    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2130    4488    4573    5644    2130 
   Size:       2358      85    1071    2913

  Fragments arranged by size: 

                2913    2358    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2947    3938    4031    2947 
   Size:        991      93    5343

  Fragments arranged by size: 

                5343     991      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3219    3219 
   Size:       6427
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2130    2727    3643    3746    4488    4573    5644 
   Size:        399     340     597     916     103     742      85    1071

Cuts at:   5644    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     916     742     597     399     340     103
                  85
 
BspEI T'CCGG_A

Cuts at:   2703    6090    2703 
   Size:       3387    3040
 
BspHI T'CATG_A

Cuts at:   2596    2694    4316    6046    2596 
   Size:         98    1622    1730    2977

  Fragments arranged by size: 

                2977    1730    1622      98
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3782     844 
   Size:       2938    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2181    4314    1394 
   Size:        429     358    2133    3507

  Fragments arranged by size: 

                3507    2133     429     358
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2022    4922    5096      65 
   Size:       1183     774    2900     174    1396

  Fragments arranged by size: 

                2900    1396    1183     774     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5071    5258    1700 
   Size:        110    3261     187    2869

  Fragments arranged by size: 

                3261    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2348    3666      96 
   Size:       2252    1318    2857

  Fragments arranged by size: 

                2857    2252    1318
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6427
 
BssSI C'ACGA_G

Cuts at:   4487    5499    4487 
   Size:       1012    5415
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2966    3590     794 
   Size:       2172     624    3631

  Fragments arranged by size: 

                3631    2172     624
 
BstBI TT'CG_AA

Cuts at:   5231    5231 
   Size:       6427
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6427
 
BstYI r'GATC_y

Cuts at:   1972    3757    4124    4509    4526    5978    6064    6076    6422 
   Size:       1785     367     385      17    1452      86      12     346

Cuts at:   6422    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1785    1452     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5875
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5688

  Fragments arranged by size: 

                5688     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3943    4737    4757    3943 
   Size:        794      20    5613

  Fragments arranged by size: 

                5613     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6427
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3478    3513     529 
   Size:         35    2914      35    3443

  Fragments arranged by size: 

                3443    2914      35      35
 
DraI TTT'AAA

Cuts at:   2956    3280    3731    2956 
   Size:        324     451    5652

  Fragments arranged by size: 

                5652     451     324
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5434     809 
   Size:        507    4118    1802

  Fragments arranged by size: 

                4118    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2073    3874    4763 
   Size:         54    1425      50     275      18     243    1801     889

Cuts at:   4763    6392       8 
   Size:       1629      43

  Fragments arranged by size: 

                1801    1629    1425     889     275     243      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3874    1537 
   Size:        275    2062    4090

  Fragments arranged by size: 

                4090    2062     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2135    2204    2662    4357    1353 
   Size:        782      69     458    1695    3423

  Fragments arranged by size: 

                3423    1695     782     458      69
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5014    5388    5534     387 
   Size:       1064     138    3425     374     146    1280

  Fragments arranged by size: 

                3425    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2835    4490    5087    5874    6142    1239 
   Size:        393    1203    1655     597     787     268    1524

  Fragments arranged by size: 

                1655    1524    1203     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3413    3413 
   Size:       6427
 
EcoO109I rG'GnC_Cy

Cuts at:   2086    3515    2086 
   Size:       1429    4998
 
EcoRI G'AATT_C

Cuts at:   2498    3736    4114    2498 
   Size:       1238     378    4811

  Fragments arranged by size: 

                4811    1238     378
 
EcoRV GAT'ATC

Cuts at:   2821    2821 
   Size:       6427
 
FspI TGC'GCA

Cuts at:   4933    4933 
   Size:       6427
 
HaeII r_GCGC'y

Cuts at:   1678    2173    5574    1678 
   Size:        495    3401    2531

  Fragments arranged by size: 

                3401    2531     495
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2198    2230    2584    2616    5068 
   Size:         32     135      32     626      32     354      32    2452

Cuts at:   5068    5100    5142    5174    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2452     626     354     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3043    3771    4017     669 
   Size:        988     281    1105     728     246    3079

  Fragments arranged by size: 

                3079    1105     988     728     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2326    3175     748 
   Size:       1578     849    4000

  Fragments arranged by size: 

                4000    1578     849
 
HpaI GTT'AAC

Cuts at:   4017    4017 
   Size:       6427
 
KasI G'GCGC_C

Cuts at:   2169    2169 
   Size:       6427
 
KpnI G_GTAC'C

Cuts at:   3752    3752 
   Size:       6427
 
MfeI C'AATT_G

Cuts at:   4026    4026 
   Size:       6427
 
MluI A'CGCG_T

Cuts at:   6303    6303 
   Size:       6427
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2473    5541    5725    1305 
   Size:       1168    3068     184    2007

  Fragments arranged by size: 

                3068    2007    1168     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2075      10 
   Size:         54    1425     343     243    4362

  Fragments arranged by size: 

                4362    1425     343     243      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    4385    4744    4903     518 
   Size:        648    3219     359     159    2042

  Fragments arranged by size: 

                3219    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2139    2342    2423    3843    4520    5668    5913    1606 
   Size:        533     203      81    1420     677    1148     245    2120

  Fragments arranged by size: 

                2120    1420    1148     677     533     245     203      81
            
 
NarI GG'CG_CC

Cuts at:   2170    2170 
   Size:       6427
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5875
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6427
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6427
 
NotI GC'GGCC_GC

Cuts at:   3874    3874 
   Size:       6427
 
NspI r_CATG'y

Cuts at:   1132    1229    3785    5330    1132 
   Size:         97    2556    1545    2229

  Fragments arranged by size: 

                2556    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6427
 
PciI A'CATG_T

Cuts at:   5326    5326 
   Size:       6427
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3254    1108 
   Size:       2146    4281
 
PmeI GTTT'AAAC

Cuts at:   3731    3731 
   Size:       6427
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4496    4528    6009    6041    4496 
   Size:         32    1481      32    4882

  Fragments arranged by size: 

                4882    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2086    3515    2086 
   Size:       1429    4998
 
PshAI GACnn'nnGTC

Cuts at:   1613    2538    1613 
   Size:        925    5502
 
PsiI TTA'TAA

Cuts at:   2046    3997    2046 
   Size:       1951    4476
 
PspXI vC'TCGA_Gb

Cuts at:   2282    3030    3636    2282 
   Size:        748     606    5073

  Fragments arranged by size: 

                5073     748     606
 
PstI C_TGCA'G

Cuts at:    830    2508    3779     830 
   Size:       1678    1271    3478

  Fragments arranged by size: 

                3478    1678    1271
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6427
 
PvuII CAG'CTG

Cuts at:   2342    2423    2342 
   Size:         81    6346
 
SalI G'TCGA_C

Cuts at:   1936    3769    1936 
   Size:       1833    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2135    2662    2135 
   Size:        527    5900
 
SbfI CC_TGCA'GG

Cuts at:   3779    3779 
   Size:       6427
 
ScaI AGT'ACT

Cuts at:   3036    4675    6387    3036 
   Size:       1639    1712    3076

  Fragments arranged by size: 

                3076    1712    1639
 
SfcI C'TryA_G

Cuts at:    826    1045    2504    3775    4910    5591    5782     826 
   Size:        219    1459    1271    1135     681     191    1471

  Fragments arranged by size: 

                1471    1459    1271    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2171    2171 
   Size:       6427
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6427
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6427
 
SmaI CCC'GGG

Cuts at:   1872    3754    1872 
   Size:       1882    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2282    3030    3636    4531    5432    5694 
   Size:        197     926     339     748     606     895     901     262

Cuts at:   5694     820 
   Size:       1553

  Fragments arranged by size: 

                1553     926     901     895     748     606     339     262
                 197
 
SnaBI TAC'GTA

Cuts at:    493    4218     493 
   Size:       3725    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6427
 
SphI G_CATG'C

Cuts at:   1132    3785    1132 
   Size:       2653    3774
 
SspI AAT'ATT

Cuts at:      5      52    2314    3687    4351       5 
   Size:         47    2262    1373     664    2081

  Fragments arranged by size: 

                2262    2081    1373     664      47
 
StuI AGG'CCT

Cuts at:   1517    2220    1517 
   Size:        703    5724
 
StyI C'CwwG_G

Cuts at:    513    1065    2536    3062    3620    3866     513 
   Size:        552    1471     526     558     246    3074

  Fragments arranged by size: 

                3074    1471     558     552     526     246
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4454    4471    4624    4809    5254     325 
   Size:       1255    2874      17     153     185     445    1498

  Fragments arranged by size: 

                2874    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2348    2796    3034 
   Size:        274      80      33      51    1443     371     448     238

Cuts at:   3034    3666    4673    6385      96 
   Size:        632    1007    1712     138

  Fragments arranged by size: 

                1712    1443    1007     632     448     371     274     238
                 138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2385    2417    2433    2465    2538    2570    6387    6419    2385 
   Size:         32      16      32      73      32    3817      32    2393

  Fragments arranged by size: 

                3817    2393      73      32      32      32      32      16
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6427
 
XbaI T'CTAG_A

Cuts at:   3763    3763 
   Size:       6427
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2579    2579 
   Size:       6427
 
XhoI C'TCGA_G

Cuts at:   1943    2282    3030    3636    1943 
   Size:        339     748     606    4734

  Fragments arranged by size: 

                4734     748     606     339
 
XmaI C'CCGG_G

Cuts at:   1870    3752    1870 
   Size:       1882    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4556    4556 
   Size:       6427
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4235     276 
   Size:         53      83     186     936    2701    2468

  Fragments arranged by size: 

                2701    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PstI     PvuI    PvuII     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI     FalI
     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI   PspOMI     PsrI    RsrII     SacI    SacII    SanDI    SexAI
     SfiI     SrfI     SwaI
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