(Circular) (Six-base) MAPSORT of: pFN21AE0025.seq Check: 5072 from: 1 to: 6427 pFN21AE0025 6427 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3770 1937 Size: 1833 4594 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4490 5874 6142 1632 Size: 2858 1384 268 1917 Fragments arranged by size: 2858 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 5326 6303 1203 Size: 4123 977 1327 Fragments arranged by size: 4123 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6427 AlwNI CAG_nnn'CTG Cuts at: 4170 5742 4170 Size: 1572 4855 ApaLI G'TGCA_C Cuts at: 4484 5640 4484 Size: 1156 5271 ApoI r'AATT_y Cuts at: 1493 2498 3067 3093 3736 3962 4114 6223 1493 Size: 1005 569 26 643 226 152 2109 1697 Fragments arranged by size: 2109 1697 1005 643 569 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2165 2282 2989 3030 3636 3752 1870 Size: 73 222 117 707 41 606 116 4545 Fragments arranged by size: 4545 707 606 222 117 116 73 41 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 3748 5203 618 Size: 325 217 627 382 1579 1455 1842 Fragments arranged by size: 1842 1579 1455 627 382 325 217 BanII G_rGCy'C Cuts at: 1391 2727 3643 3746 1391 Size: 1336 916 103 4072 Fragments arranged by size: 4072 1336 916 103 BbeI G_GCGC'C Cuts at: 2173 2173 Size: 6427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3491 3525 4618 4652 1903 Size: 34 1554 34 1093 34 3678 Fragments arranged by size: 3678 1554 1093 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3326 4319 5529 3326 Size: 993 1210 4224 Fragments arranged by size: 4224 1210 993 BclI T'GATC_A Cuts at: 1548 2599 3112 1548 Size: 1051 513 4863 Fragments arranged by size: 4863 1051 513 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5038 136 Size: 107 122 71 631 605 3366 1525 Fragments arranged by size: 3366 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 4488 5644 1790 Size: 2698 1156 2573 Fragments arranged by size: 2698 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2182 3379 4158 Size: 416 53 83 399 24 765 1197 779 Cuts at: 4158 5116 442 Size: 958 1753 Fragments arranged by size: 1753 1197 958 779 765 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3662 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2835 5087 1239 Size: 1596 2252 2579 Fragments arranged by size: 2579 2252 1596 Bpu10I CC'TnA_GC Cuts at: 3414 3414 Size: 6427 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4552 5417 5715 4552 Size: 865 298 5264 Fragments arranged by size: 5264 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2165 2896 5090 882 Size: 617 666 731 2194 2219 Fragments arranged by size: 2219 2194 731 666 617 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2170 4234 4616 5251 Size: 53 83 186 936 637 2064 382 635 Cuts at: 5251 275 Size: 1451 Fragments arranged by size: 2064 1451 936 637 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2703 4860 5258 5532 5679 6090 2703 Size: 2157 398 274 147 411 3040 Fragments arranged by size: 3040 2157 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 2385 2415 2538 Size: 30 165 30 672 30 280 30 123 Cuts at: 2538 2568 1178 Size: 30 5037 Fragments arranged by size: 5037 672 280 165 123 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 3066 1186 Size: 967 913 4547 Fragments arranged by size: 4547 967 913 BseYI C'CCAG_C Cuts at: 2116 5630 6126 2116 Size: 3514 496 2417 Fragments arranged by size: 3514 2417 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2678 1163 Size: 573 942 4912 Fragments arranged by size: 4912 942 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2130 4488 4573 5644 2130 Size: 2358 85 1071 2913 Fragments arranged by size: 2913 2358 1071 85 BsmI GAATG_Cn' Cuts at: 2947 3938 4031 2947 Size: 991 93 5343 Fragments arranged by size: 5343 991 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3219 3219 Size: 6427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2727 3643 3746 4488 4573 5644 Size: 399 340 597 916 103 742 85 1071 Cuts at: 5644 1391 Size: 2174 Fragments arranged by size: 2174 1071 916 742 597 399 340 103 85 BspEI T'CCGG_A Cuts at: 2703 6090 2703 Size: 3387 3040 BspHI T'CATG_A Cuts at: 2596 2694 4316 6046 2596 Size: 98 1622 1730 2977 Fragments arranged by size: 2977 1730 1622 98 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3782 844 Size: 2938 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2181 4314 1394 Size: 429 358 2133 3507 Fragments arranged by size: 3507 2133 429 358 BsrDI GCAATG_nn' Cuts at: 65 1248 2022 4922 5096 65 Size: 1183 774 2900 174 1396 Fragments arranged by size: 2900 1396 1183 774 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5071 5258 1700 Size: 110 3261 187 2869 Fragments arranged by size: 3261 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2348 3666 96 Size: 2252 1318 2857 Fragments arranged by size: 2857 2252 1318 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 2966 3590 794 Size: 2172 624 3631 Fragments arranged by size: 3631 2172 624 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6427 BstYI r'GATC_y Cuts at: 1972 3757 4124 4509 4526 5978 6064 6076 6422 Size: 1785 367 385 17 1452 86 12 346 Cuts at: 6422 1972 Size: 1977 Fragments arranged by size: 1977 1785 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5875 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5688 Fragments arranged by size: 5688 683 56 BtsI GCAGTG_nn' Cuts at: 3943 4737 4757 3943 Size: 794 20 5613 Fragments arranged by size: 5613 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3478 3513 529 Size: 35 2914 35 3443 Fragments arranged by size: 3443 2914 35 35 DraI TTT'AAA Cuts at: 2956 3280 3731 2956 Size: 324 451 5652 Fragments arranged by size: 5652 451 324 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 3874 4763 Size: 54 1425 50 275 18 243 1801 889 Cuts at: 4763 6392 8 Size: 1629 43 Fragments arranged by size: 1801 1629 1425 889 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 2204 2662 4357 1353 Size: 782 69 458 1695 3423 Fragments arranged by size: 3423 1695 782 458 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2835 4490 5087 5874 6142 1239 Size: 393 1203 1655 597 787 268 1524 Fragments arranged by size: 1655 1524 1203 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3413 3413 Size: 6427 EcoO109I rG'GnC_Cy Cuts at: 2086 3515 2086 Size: 1429 4998 EcoRI G'AATT_C Cuts at: 2498 3736 4114 2498 Size: 1238 378 4811 Fragments arranged by size: 4811 1238 378 EcoRV GAT'ATC Cuts at: 2821 2821 Size: 6427 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2173 5574 1678 Size: 495 3401 2531 Fragments arranged by size: 3401 2531 495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2584 2616 5068 Size: 32 135 32 626 32 354 32 2452 Cuts at: 5068 5100 5142 5174 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2452 626 354 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3043 3771 4017 669 Size: 988 281 1105 728 246 3079 Fragments arranged by size: 3079 1105 988 728 281 246 HindIII A'AGCT_T Cuts at: 748 2326 3175 748 Size: 1578 849 4000 Fragments arranged by size: 4000 1578 849 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KasI G'GCGC_C Cuts at: 2169 2169 Size: 6427 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 4026 4026 Size: 6427 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2473 5541 5725 1305 Size: 1168 3068 184 2007 Fragments arranged by size: 3068 2007 1168 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 10 Size: 54 1425 343 243 4362 Fragments arranged by size: 4362 1425 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 4385 4744 4903 518 Size: 648 3219 359 159 2042 Fragments arranged by size: 3219 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2139 2342 2423 3843 4520 5668 5913 1606 Size: 533 203 81 1420 677 1148 245 2120 Fragments arranged by size: 2120 1420 1148 677 533 245 203 81 NarI GG'CG_CC Cuts at: 2170 2170 Size: 6427 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5875 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 3785 5330 1132 Size: 97 2556 1545 2229 Fragments arranged by size: 2556 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6427 PciI A'CATG_T Cuts at: 5326 5326 Size: 6427 PflMI CCAn_nnn'nTGG Cuts at: 1108 3254 1108 Size: 2146 4281 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 3515 2086 Size: 1429 4998 PshAI GACnn'nnGTC Cuts at: 1613 2538 1613 Size: 925 5502 PsiI TTA'TAA Cuts at: 2046 3997 2046 Size: 1951 4476 PspXI vC'TCGA_Gb Cuts at: 2282 3030 3636 2282 Size: 748 606 5073 Fragments arranged by size: 5073 748 606 PstI C_TGCA'G Cuts at: 830 2508 3779 830 Size: 1678 1271 3478 Fragments arranged by size: 3478 1678 1271 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2342 2423 2342 Size: 81 6346 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SapI GCTCTTCn'nnn_ Cuts at: 2135 2662 2135 Size: 527 5900 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 3036 4675 6387 3036 Size: 1639 1712 3076 Fragments arranged by size: 3076 1712 1639 SfcI C'TryA_G Cuts at: 826 1045 2504 3775 4910 5591 5782 826 Size: 219 1459 1271 1135 681 191 1471 Fragments arranged by size: 1471 1459 1271 1135 681 219 191 SfoI GGC'GCC Cuts at: 2171 2171 Size: 6427 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 3030 3636 4531 5432 5694 Size: 197 926 339 748 606 895 901 262 Cuts at: 5694 820 Size: 1553 Fragments arranged by size: 1553 926 901 895 748 606 339 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 2314 3687 4351 5 Size: 47 2262 1373 664 2081 Fragments arranged by size: 2262 2081 1373 664 47 StuI AGG'CCT Cuts at: 1517 2220 1517 Size: 703 5724 StyI C'CwwG_G Cuts at: 513 1065 2536 3062 3620 3866 513 Size: 552 1471 526 558 246 3074 Fragments arranged by size: 3074 1471 558 552 526 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4454 4471 4624 4809 5254 325 Size: 1255 2874 17 153 185 445 1498 Fragments arranged by size: 2874 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2348 2796 3034 Size: 274 80 33 51 1443 371 448 238 Cuts at: 3034 3666 4673 6385 96 Size: 632 1007 1712 138 Fragments arranged by size: 1712 1443 1007 632 448 371 274 238 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2385 2417 2433 2465 2538 2570 6387 6419 2385 Size: 32 16 32 73 32 3817 32 2393 Fragments arranged by size: 3817 2393 73 32 32 32 32 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2579 2579 Size: 6427 XhoI C'TCGA_G Cuts at: 1943 2282 3030 3636 1943 Size: 339 748 606 4734 Fragments arranged by size: 4734 748 606 339 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 4556 4556 Size: 6427 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI