Restriction map for
pFN21AE0025
(Linear) (Six-base) MAPSORT of: KIEE0025.seq Check: 9575 from: 1 to: 1722
KIEE0025.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
ApoI r'AATT_y
Cuts at: 0 493 1062 1088 1722
Size: 493 569 26 634
Fragments arranged by size:
634 569 493 26
AvaI C'yCGr_G
Cuts at: 0 160 277 984 1025 1631 1722
Size: 160 117 707 41 606 91
Fragments arranged by size:
707 606 160 117 91 41
BanI G'GyrC_C
Cuts at: 0 164 1722
Size: 164 1558
BanII G_rGCy'C
Cuts at: 0 722 1638 1722
Size: 722 916 84
Fragments arranged by size:
916 722 84
BbeI G_GCGC'C
Cuts at: 0 168 1722
Size: 168 1554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1486 1520 1722
Size: 1486 34 202
Fragments arranged by size:
1486 202 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1321 1722
Size: 1321 401
BclI T'GATC_A
Cuts at: 0 594 1107 1722
Size: 594 513 615
Fragments arranged by size:
615 594 513
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 1374 1722
Size: 177 1197 348
Fragments arranged by size:
1197 348 177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1657 1722
Size: 1657 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1722
Size: 830 892
Bpu10I CC'TnA_GC
Cuts at: 0 1409 1722
Size: 1409 313
BsaI GGTCTCn'nnnn_
Cuts at: 0 160 891 1722
Size: 160 731 831
Fragments arranged by size:
831 731 160
BsaHI Gr'CG_yC
Cuts at: 0 165 1722
Size: 165 1557
BsaWI w'CCGG_w
Cuts at: 0 698 1722
Size: 698 1024
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 380 410 533 563 1722
Size: 70 30 280 30 123 30 1159
Fragments arranged by size:
1159 280 123 70 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 1061 1722
Size: 148 913 661
Fragments arranged by size:
913 661 148
BseYI C'CCAG_C
Cuts at: 0 111 1722
Size: 111 1611
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1722
Size: 673 1049
BsiHKAI G_wGCw'C
Cuts at: 0 125 1722
Size: 125 1597
BsmI GAATG_Cn'
Cuts at: 0 942 1722
Size: 942 780
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1214 1722
Size: 1214 508
Bsp1286I G_dGCh'C
Cuts at: 0 125 722 1638 1722
Size: 125 597 916 84
Fragments arranged by size:
916 597 125 84
BspEI T'CCGG_A
Cuts at: 0 698 1722
Size: 698 1024
BspHI T'CATG_A
Cuts at: 0 591 689 1722
Size: 591 98 1033
Fragments arranged by size:
1033 591 98
BsrBI CCG'CTC
Cuts at: 0 176 1722
Size: 176 1546
BsrDI GCAATG_nn'
Cuts at: 0 17 1722
Size: 17 1705
BsrGI T'GTAC_A
Cuts at: 0 343 1661 1722
Size: 343 1318 61
Fragments arranged by size:
1318 343 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 961 1585 1722
Size: 961 624 137
Fragments arranged by size:
961 624 137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1473 1508 1722
Size: 1473 35 214
Fragments arranged by size:
1473 214 35
DraI TTT'AAA
Cuts at: 0 951 1275 1722
Size: 951 324 447
Fragments arranged by size:
951 447 324
EaeI y'GGCC_r
Cuts at: 0 68 1722
Size: 68 1654
EarI CTCTTCn'nnn_
Cuts at: 0 130 199 657 1722
Size: 130 69 458 1065
Fragments arranged by size:
1065 458 130 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1722
Size: 830 892
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1408 1722
Size: 1408 314
EcoO109I rG'GnC_Cy
Cuts at: 0 81 1510 1722
Size: 81 1429 212
Fragments arranged by size:
1429 212 81
EcoRI G'AATT_C
Cuts at: 0 493 1722
Size: 493 1229
EcoRV GAT'ATC
Cuts at: 0 816 1722
Size: 816 906
HaeII r_GCGC'y
Cuts at: 0 168 1722
Size: 168 1554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 579 611 1722
Size: 193 32 354 32 1111
Fragments arranged by size:
1111 354 193 32 32
HincII GTy'rAC
Cuts at: 0 1038 1722
Size: 1038 684
HindIII A'AGCT_T
Cuts at: 0 321 1170 1722
Size: 321 849 552
Fragments arranged by size:
849 552 321
KasI G'GCGC_C
Cuts at: 0 164 1722
Size: 164 1558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1722
Size: 468 1254
MscI TGG'CCA
Cuts at: 0 70 1722
Size: 70 1652
MspA1I CmG'CkG
Cuts at: 0 134 337 418 1722
Size: 134 203 81 1304
Fragments arranged by size:
1304 203 134 81
NarI GG'CG_CC
Cuts at: 0 165 1722
Size: 165 1557
PflMI CCAn_nnn'nTGG
Cuts at: 0 1249 1722
Size: 1249 473
PpuMI rG'GwC_Cy
Cuts at: 0 81 1510 1722
Size: 81 1429 212
Fragments arranged by size:
1429 212 81
PshAI GACnn'nnGTC
Cuts at: 0 533 1722
Size: 533 1189
PsiI TTA'TAA
Cuts at: 0 41 1722
Size: 41 1681
PspXI vC'TCGA_Gb
Cuts at: 0 277 1025 1631 1722
Size: 277 748 606 91
Fragments arranged by size:
748 606 277 91
PstI C_TGCA'G
Cuts at: 0 503 1722
Size: 503 1219
PvuII CAG'CTG
Cuts at: 0 337 418 1722
Size: 337 81 1304
Fragments arranged by size:
1304 337 81
SapI GCTCTTCn'nnn_
Cuts at: 0 130 657 1722
Size: 130 527 1065
Fragments arranged by size:
1065 527 130
ScaI AGT'ACT
Cuts at: 0 1031 1722
Size: 1031 691
SfcI C'TryA_G
Cuts at: 0 499 1722
Size: 499 1223
SfoI GGC'GCC
Cuts at: 0 166 1722
Size: 166 1556
SmlI C'TyrA_G
Cuts at: 0 277 1025 1631 1722
Size: 277 748 606 91
Fragments arranged by size:
748 606 277 91
SspI AAT'ATT
Cuts at: 0 309 1682 1722
Size: 309 1373 40
Fragments arranged by size:
1373 309 40
StuI AGG'CCT
Cuts at: 0 215 1722
Size: 215 1507
StyI C'CwwG_G
Cuts at: 0 531 1057 1615 1722
Size: 531 526 558 107
Fragments arranged by size:
558 531 526 107
TatI w'GTAC_w
Cuts at: 0 343 791 1029 1661 1722
Size: 343 448 238 632 61
Fragments arranged by size:
632 448 343 238 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 380 412 428 460 533 565 1722
Size: 380 32 16 32 73 32 1157
Fragments arranged by size:
1157 380 73 32 32 32 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 574 1722
Size: 574 1148
XhoI C'TCGA_G
Cuts at: 0 277 1025 1631 1722
Size: 277 748 606 91
Fragments arranged by size:
748 606 277 91
Enzymes that do cut:
ApoI AvaI BanI BanII BbeI BcgI BciVI BclI
BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrGI BstAPI CspCI DraI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
HincII HindIII KasI MmeI MscI MspA1I NarI PflMI
PpuMI PshAI PsiI PspXI PstI PvuII SapI ScaI
SfcI SfoI SmlI SspI StuI StyI TatI TstI
XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BpuEI BsaAI BsaBI BsiEI BsiWI BspMI BsrFI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraIII DrdI EagI EciI EcoICRI
FalI FseI FspI FspAI HpaI KpnI MfeI MluI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PspOMI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII Tth111I XbaI XmaI
XmnI ZraI