Restriction map for
pFN21AB5713
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstEII
BstPI Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 1541 CGCG CG^CG
AflIII 147 ACATGT A^C[GA][CT]GT
Alw44I 2227 GTGCAC G^TGCAC
ApaLI 2227 GTGCAC G^TGCAC
AvaII 39 GGACC G^G[AT]CC
BalI 1775 TGGCCA TGG^CCA
BcnI 36 CCCGG CC^[GC]GG
BglII 407 AGATCT A^GATCT
BpmI 2092 CTGGAG CTGGAG(16/14)
BsmFI 1594 GGGAC GGGAC(10/14)
BsmI 859 GAATGC GAATGC(1/-1)
Bsp1407I 2280 TGTACA T^GTACA
BspLU11I 147 ACATGT A^CATGT
BsrGI 2280 TGTACA T^GTACA
Bst1107I 1859 GTATAC GTA^TAC
BstAPI 1147 GCACATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1192 TTCGAA TT^CGAA
BstUI 1541 CGCG CG^CG
BstYI 407 AGATCT [GA]^GATC[CT]
BstZ17I 1859 GTATAC GTA^TAC
DraI 2101 TTTAAA TTT^AAA
DsaI 1947 CCGTGG C^C[GA][CT]GG
EcoNI 2043 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 37 CCGG C^CGG
HincII 490 GTCAAC GT[CT]^[GA]AC
HindII 490 GTCAAC GT[CT]^[GA]AC
HpaII 37 CCGG C^CGG
MaeII 226 ACGT A^CGT
MflI 407 AGATCT [GA]^GATC[CT]
MluNI 1775 TGGCCA TGG^CCA
MscI 1775 TGGCCA TGG^CCA
MspI 37 CCGG C^CGG
MvnI 1541 CGCG CG^CG
NciI 36 CCCGG CC^[GC]GG
NspI 147 ACATGT [GA]CATG^[CT]
NspV 1192 TTCGAA TT^CGAA
PstI 2047 CTGCAG CTGCA^G
SacI 1973 GAGCTC GAGCT^C
SbfI 2046 CCTGCAGG CCTGCA^GG
ScaI 833 AGTACT AGT^ACT
SfuI 1192 TTCGAA TT^CGAA
Sse8387I 2046 CCTGCAGG CCTGCA^GG
SspBI 2280 TGTACA T^GTACA
SspI 636 AATATT AAT^ATT
Tsp45I 1169 GTCAC ^GT[GC]AC
XhoII 407 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 793 GTCTAC GT^[AC][GT]AC
1859 GTATAC
AlwI 1483 GATCC GGATC(4/5)
1387 GGATC
AlwNI 295 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2007 CAGGGGCTG
Asp700I 1185 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2134 GAAATGATTC
AspHI 1973 GAGCTC G[AT]GC[AT]^C
2227 GTGCAC
BbvCI 109 GCTGAGG CCTCAGC(-5/-2)
1348 GCTGAGG
BfaI 589 CTAG C^TAG
1417 CTAG
BsaI 272 GAGACC GGTCTC(1/5)
1399 GAGACC
BseRI 1526 CTCCTC GAGGAG(10/8)
1714 GAGGAG
BsgI 1046 GTGCAG GTGCAG(16/14)
1337 GTGCAG
BsiHKAI 1973 GAGCTC G[AT]GC[AT]^C
2227 GTGCAC
BspHI 1308 TCATGA T^CATGA
1511 TCATGA
BstXI 721 CCAGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2232 CCATCCAGGTGG
Cfr13I 39 GGACC G^G[ACGT]CC
55 GGCCC
EaeI 718 CGGCCA [CT]^GGCC[GA]
1775 TGGCCA
HaeII 12 AGCGCC [GA]GCGC^[CT]
60 AGCGCC
MaeI 589 CTAG C^TAG
1417 CTAG
MaeIII 334 GTTAC ^GT[ACGT]AC
1169 GTCAC
PleI 1806 GAGTC GAGTC(4/5)
1986 GAGTC
RcaI 1308 TCATGA T^CATGA
1511 TCATGA
Sau96I 39 GGACC G^G[ACGT]CC
55 GGCCC
SfaNI 106 GATGC GCATC(5/9)
1111 GCATC
SmlI 1180 CTCAAG C^T[CT][GA]AG
1895 CTCAAG
TaqI 1193 TCGA T^CGA
2307 TCGA
TthHB8I 1193 TCGA T^CGA
2307 TCGA
XmnI 1185 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2134 GAAATGATTC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanII BbsI BbvI BmyI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
Ksp632I MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI SfcI
TfiI Tru9I TseI Tsp509I TspRI