Restriction map for
pFN21AB8748
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# NO CUT ENZYMES ( 200 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BmyI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI Ksp632I KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tru9I Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 444 GGTACC G^GTACC
AocI 85 CCTCAGG CC^T[ACGT]AGG
Asp718I 444 GGTACC G^GTACC
BbsI 492 GAAGAC GAAGAC(2/6)
BbvCI 40 CCTCAGC CCTCAGC(-5/-2)
BciVI 409 GGATAC GTATCC(6/5)
BlpI 484 GCTCAGC GC^T[ACGT]AGC
BpmI 473 CTCCAG CTGGAG(16/14)
Bpu1102I 484 GCTCAGC GC^T[ACGT]AGC
BpuAI 492 GAAGAC GAAGAC(2/6)
BspMI 57 GCAGGT ACCTGC(4/8)
Bsu36I 85 CCTCAGG CC^T[ACGT]AGG
Cac8I 74 GCCAGC GC[ACGT]^[ACGT]GC
CelII 484 GCTCAGC GC^T[ACGT]AGC
Eco81I 85 CCTCAGG CC^T[ACGT]AGG
HaeIII 478 GGCC GG^CC
HinfI 156 GATTC G^A[ACGT]TC
HphI 60 GGTGA GGTGA(8/7)
KpnI 444 GGTACC GGTAC^C
MaeIII 469 GTCAC ^GT[ACGT]AC
MboII 492 GAAGA GAAGA(8/7)
MwoI 45 GCTTCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 136 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 341 GCATC GCATC(5/9)
SspI 93 AATATT AAT^ATT
TfiI 156 GATTC G^A[AT]TC
Tsp45I 469 GTCAC ^GT[GC]AC
Van91I 136 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 7 enzymes) #
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AluI 44 AGCT AG^CT
483 AGCT
BanI 385 GGTGCC G^G[CT][GA]CC
444 GGTACC
BbvI 54 GCAGC GCAGC(8/12)
338 GCAGC
Fnu4HI 54 GCAGC GC^[ACGT]GC
338 GCAGC
ItaI 54 GCAGC GC^[ACGT]GC
338 GCAGC
NlaIII 119 CATG CATG^
455 CATG
TseI 54 GCAGC G^C[AT]GC
338 GCAGC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AvaII BsaJI BsiYI BslI
BsmFI BssKI BstNI Cfr13I DdeI
DraII EcoNI EcoO109I EcoRII EcoT14I
MnlI MvaI NlaIV PpuMI RsaI
Sau96I ScrFI StyI