(Circular) (Six-base) MAPSORT of: pFN21AB9918.seq Check: 3874 from: 1 to: 6442 pFN21AB9918 6442 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2640 4252 278 Size: 53 83 186 936 1104 1612 2468 Fragments arranged by size: 2468 1612 1104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 3785 1937 Size: 72 1776 4594 Fragments arranged by size: 4594 1776 72 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 2619 2626 3723 4505 5889 6157 1632 Size: 714 273 7 1097 782 1384 268 1917 Fragments arranged by size: 1917 1384 1097 782 714 273 268 7 AfeI AGC'GCT Cuts at: 2457 3044 2457 Size: 587 5855 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 2675 3177 5341 6318 1203 Size: 1472 502 2164 977 1327 Fragments arranged by size: 2164 1472 1327 977 502 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3036 1184 Size: 1852 4590 AlwNI CAG_nnn'CTG Cuts at: 2018 3272 4185 5757 2018 Size: 1254 913 1572 2703 Fragments arranged by size: 2703 1572 1254 913 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 2520 2764 3751 3977 4129 6238 1493 Size: 1027 244 987 226 152 2109 1697 Fragments arranged by size: 2109 1697 1027 987 244 226 152 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 3767 1870 Size: 73 1824 4545 Fragments arranged by size: 4545 1824 73 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2593 3763 5218 618 Size: 325 217 627 806 1170 1455 1842 Fragments arranged by size: 1842 1455 1170 806 627 325 217 BanII G_rGCy'C Cuts at: 1391 2336 3761 1391 Size: 945 1425 4072 Fragments arranged by size: 4072 1425 945 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2034 2632 928 Size: 971 135 598 4738 Fragments arranged by size: 4738 971 598 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4633 4667 1903 Size: 34 2696 34 3678 Fragments arranged by size: 3678 2696 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2913 4334 5544 2913 Size: 1421 1210 3811 Fragments arranged by size: 3811 1421 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6442 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5053 136 Size: 107 122 71 631 605 3381 1525 Fragments arranged by size: 3381 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6437 6437 Size: 6442 BlpI GC'TnA_GC Cuts at: 3859 3859 Size: 6442 Bme1580I G_kGCm'C Cuts at: 1790 3464 4503 5659 1790 Size: 1674 1039 1156 2573 Fragments arranged by size: 2573 1674 1156 1039 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3297 4173 5131 Size: 416 53 83 399 24 1880 876 958 Cuts at: 5131 442 Size: 1753 Fragments arranged by size: 1880 1753 958 876 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5102 1239 Size: 3863 2579 Bpu10I CC'TnA_GC Cuts at: 2772 2772 Size: 6442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4567 5432 5730 4567 Size: 865 298 5279 Fragments arranged by size: 5279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5105 882 Size: 617 3606 2219 Fragments arranged by size: 3606 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4233 493 Size: 711 3029 2702 Fragments arranged by size: 3029 2702 711 BsaBI GATnn'nnATC Cuts at: 6436 6436 Size: 6442 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2637 4249 4631 5266 Size: 53 83 186 936 1104 1612 382 635 Cuts at: 5266 275 Size: 1451 Fragments arranged by size: 1612 1451 1104 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4875 5273 5547 5694 6105 4875 Size: 398 274 147 411 5212 Fragments arranged by size: 5212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6217 Fragments arranged by size: 6217 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2017 2020 2023 2026 2151 1186 Size: 831 3 3 3 125 5477 Fragments arranged by size: 5477 831 125 3 3 3 BseYI C'CCAG_C Cuts at: 2646 2707 2820 5645 6141 2646 Size: 61 113 2825 496 2947 Fragments arranged by size: 2947 2825 496 113 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5869 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3892 4653 5681 1540 Size: 275 186 48 1843 761 1028 2301 Fragments arranged by size: 2301 1843 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 4503 4588 5659 4503 Size: 85 1071 5286 Fragments arranged by size: 5286 1071 85 BsmI GAATG_Cn' Cuts at: 3953 4046 3953 Size: 93 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2336 3464 3761 4503 4588 5659 1391 Size: 399 546 1128 297 742 85 1071 2174 Fragments arranged by size: 2174 1128 1071 742 546 399 297 85 BspEI T'CCGG_A Cuts at: 6105 6105 Size: 6442 BspHI T'CATG_A Cuts at: 4331 6061 4331 Size: 1730 4712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3797 844 Size: 2953 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4329 1394 Size: 429 2506 3507 Fragments arranged by size: 3507 2506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2366 2400 2967 3689 4937 5111 65 Size: 1183 1118 34 567 722 1248 174 1396 Fragments arranged by size: 1396 1248 1183 1118 722 567 174 34 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 5086 5273 1700 Size: 110 238 3038 187 2869 Fragments arranged by size: 3038 2869 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 4502 5514 4502 Size: 1012 5430 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6442 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6442 BstXI CCAn_nnnn'nTGG Cuts at: 3415 3415 Size: 6442 BstYI r'GATC_y Cuts at: 1972 2812 3236 3772 4139 4524 4541 5993 6079 Size: 840 424 536 367 385 17 1452 86 Cuts at: 6079 6091 6437 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 840 536 424 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3325 3325 Size: 6442 BtgI C'CryG_G Cuts at: 513 1065 3118 3362 513 Size: 552 2053 244 3593 Fragments arranged by size: 3593 2053 552 244 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5703 Fragments arranged by size: 5703 683 56 BtsI GCAGTG_nn' Cuts at: 3133 3492 3958 4752 4772 3133 Size: 359 466 794 20 4803 Fragments arranged by size: 4803 794 466 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 3746 3746 Size: 6442 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5449 809 Size: 507 4133 1802 Fragments arranged by size: 4133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3889 4778 Size: 54 1425 50 275 18 216 1843 889 Cuts at: 4778 6407 8 Size: 1629 43 Fragments arranged by size: 1843 1629 1425 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3889 1537 Size: 275 234 1843 4090 Fragments arranged by size: 4090 1843 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 2115 2128 4372 1353 Size: 732 30 13 2244 3423 Fragments arranged by size: 3423 2244 732 30 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5029 5403 5549 387 Size: 1064 138 3440 374 146 1280 Fragments arranged by size: 3440 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2346 2619 2626 3723 4505 5102 5889 Size: 393 714 273 7 1097 782 597 787 Cuts at: 5889 6157 1239 Size: 268 1524 Fragments arranged by size: 1524 1097 787 782 714 597 393 273 268 7 EcoNI CCTnn'n_nnAGG Cuts at: 3626 3626 Size: 6442 EcoO109I rG'GnC_Cy Cuts at: 2077 2470 2077 Size: 393 6049 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2459 2827 3046 5589 1678 Size: 781 368 219 2543 2531 Fragments arranged by size: 2543 2531 781 368 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2662 2694 5083 5115 5157 Size: 32 135 32 1090 32 2389 32 42 Cuts at: 5157 5189 1373 Size: 32 2626 Fragments arranged by size: 2626 2389 1090 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3786 4032 669 Size: 988 281 1848 246 3079 Fragments arranged by size: 3079 1848 988 281 246 HindIII A'AGCT_T Cuts at: 748 2276 3103 3641 748 Size: 1528 827 538 3549 Fragments arranged by size: 3549 1528 827 538 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 2675 6318 2675 Size: 3643 2799 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 2660 5556 5740 1305 Size: 805 550 2896 184 2007 Fragments arranged by size: 2896 2007 805 550 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4620 Fragments arranged by size: 4620 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3005 3413 4400 4759 4918 518 Size: 648 1839 408 987 359 159 2042 Fragments arranged by size: 2042 1839 987 648 408 359 159 MspA1I CmG'CkG Cuts at: 1606 2646 3858 4535 5683 5928 1606 Size: 1040 1212 677 1148 245 2120 Fragments arranged by size: 2120 1212 1148 1040 677 245 NcoI C'CATG_G Cuts at: 513 1065 3362 513 Size: 552 2297 3593 Fragments arranged by size: 3593 2297 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NspI r_CATG'y Cuts at: 1132 1229 2446 3181 3800 5345 1132 Size: 97 1217 735 619 1545 2229 Fragments arranged by size: 2229 1545 1217 735 619 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 3177 5341 3177 Size: 2164 4278 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 1108 Size: 1317 5125 PfoI T'CCnGG_A Cuts at: 2878 2878 Size: 6442 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2077 2470 2077 Size: 393 6049 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PstI C_TGCA'G Cuts at: 830 3794 830 Size: 2964 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 PvuII CAG'CTG Cuts at: 2646 2646 Size: 6442 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SexAI A'CCwGG_T Cuts at: 3271 3271 Size: 6442 SfcI C'TryA_G Cuts at: 826 1045 2010 3443 3790 4925 5606 5797 826 Size: 219 965 1433 347 1135 681 191 1471 Fragments arranged by size: 1471 1433 1135 965 681 347 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4546 5447 5709 820 Size: 197 926 2603 901 262 1553 Fragments arranged by size: 2603 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 3571 152 Size: 3419 3023 SphI G_CATG'C Cuts at: 1132 2446 3800 1132 Size: 1314 1354 3774 Fragments arranged by size: 3774 1354 1314 SspI AAT'ATT Cuts at: 5 52 2269 4366 5 Size: 47 2217 2097 2081 Fragments arranged by size: 2217 2097 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6442 StyI C'CwwG_G Cuts at: 513 1065 2419 3362 3881 513 Size: 552 1354 943 519 3074 Fragments arranged by size: 3074 1354 943 552 519 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4469 4486 4639 4824 5269 325 Size: 1255 2889 17 153 185 445 1498 Fragments arranged by size: 2889 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2948 4688 6400 Size: 274 80 33 51 1443 971 1740 1712 Cuts at: 6400 96 Size: 138 Fragments arranged by size: 1740 1712 1443 971 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6402 6434 6402 Size: 32 6410 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6442 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3478 3478 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 4571 4571 Size: 6442 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2638 4250 276 Size: 53 83 186 936 1104 1612 2468 Fragments arranged by size: 2468 1612 1104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI