(Circular) (Six-base) MAPSORT of: pFN21AB9910.seq Check: 6523 from: 1 to: 6475 pFN21AB9910 6475 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3392 3519 3392 Size: 127 6348 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3624 4285 278 Size: 53 83 186 936 2088 661 2468 Fragments arranged by size: 2468 2088 936 661 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 2553 3695 3796 2553 Size: 1142 101 5232 Fragments arranged by size: 5232 1142 101 AclI AA'CG_TT Cuts at: 1563 3774 4602 4975 1563 Size: 2211 828 373 3063 Fragments arranged by size: 3063 2211 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 2436 2514 3173 3308 4538 5922 6190 Size: 590 214 78 659 135 1230 1384 268 Cuts at: 6190 1632 Size: 1917 Fragments arranged by size: 1917 1384 1230 659 590 268 214 135 78 AfeI AGC'GCT Cuts at: 2804 2804 Size: 6475 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 2712 5374 6351 1203 Size: 1509 2662 977 1327 Fragments arranged by size: 2662 1509 1327 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 2270 2573 3611 1166 Size: 1104 303 1038 4030 Fragments arranged by size: 4030 1104 1038 303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2823 1184 Size: 1639 4836 AlwNI CAG_nnn'CTG Cuts at: 2880 3384 3397 3412 4218 5790 2880 Size: 504 13 15 806 1572 3565 Fragments arranged by size: 3565 1572 806 504 15 13 ApaLI G'TGCA_C Cuts at: 4532 5688 4532 Size: 1156 5319 ApoI r'AATT_y Cuts at: 1493 2153 3784 4010 4162 6271 1493 Size: 660 1631 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 660 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 2163 2608 2891 3800 1870 Size: 73 220 445 283 909 4545 Fragments arranged by size: 4545 909 445 283 220 73 BamHI G'GATC_C Cuts at: 3805 4172 3805 Size: 367 6108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 2553 3695 3796 5251 Size: 325 217 627 237 529 1142 101 1455 Cuts at: 5251 618 Size: 1842 Fragments arranged by size: 1842 1455 1142 627 529 325 237 217 101 BanII G_rGCy'C Cuts at: 1391 2771 2917 3043 3794 1391 Size: 1380 146 126 751 4072 Fragments arranged by size: 4072 1380 751 146 126 BbeI G_GCGC'C Cuts at: 2028 2028 Size: 6475 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3491 928 Size: 971 1592 3912 Fragments arranged by size: 3912 1592 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4666 4700 1903 Size: 34 2729 34 3678 Fragments arranged by size: 3678 2729 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4367 5577 4367 Size: 1210 5265 BclI T'GATC_A Cuts at: 1548 2373 1548 Size: 825 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2885 5086 136 Size: 107 122 71 631 605 1213 2201 1525 Fragments arranged by size: 2201 1525 1213 631 605 122 107 71 BglII A'GATC_T Cuts at: 6470 6470 Size: 6475 BlpI GC'TnA_GC Cuts at: 3892 3892 Size: 6475 Bme1580I G_kGCm'C Cuts at: 1790 2858 4536 5692 1790 Size: 1068 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 1068 BmgBI CAC'GTC Cuts at: 1318 3460 1318 Size: 2142 4333 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4206 5164 442 Size: 416 53 83 399 24 2789 958 1753 Fragments arranged by size: 2789 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2214 3584 3695 5135 1239 Size: 888 87 1370 111 1440 2579 Fragments arranged by size: 2579 1440 1370 888 111 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3494 4600 5465 5763 3494 Size: 1106 865 298 4206 Fragments arranged by size: 4206 1106 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2232 2420 3101 5138 882 Size: 617 733 188 681 2037 2219 Fragments arranged by size: 2219 2037 733 681 617 188 BsaAI yAC'GTr Cuts at: 493 1204 2466 2715 4266 493 Size: 711 1262 249 1551 2702 Fragments arranged by size: 2702 1551 1262 711 249 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2025 2829 3621 4282 Size: 53 83 186 936 492 804 792 661 Cuts at: 4282 4664 5299 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 804 792 661 635 492 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2619 2974 4908 5306 5580 5727 6138 2619 Size: 355 1934 398 274 147 411 2956 Fragments arranged by size: 2956 1934 411 398 355 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 3648 3678 1178 Size: 30 165 30 1296 30 919 30 3975 Fragments arranged by size: 3975 1296 919 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2664 2771 2961 3621 1186 Size: 1478 107 190 660 4040 Fragments arranged by size: 4040 1478 660 190 107 BseYI C'CCAG_C Cuts at: 2431 3450 5678 6174 2431 Size: 1019 2228 496 2732 Fragments arranged by size: 2732 2228 1019 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 2819 3383 1163 Size: 573 615 468 564 4255 Fragments arranged by size: 4255 615 573 564 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 3925 4686 5714 1540 Size: 275 186 60 1864 761 1028 2301 Fragments arranged by size: 2301 1864 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2619 2712 3242 3447 4536 4621 5692 2619 Size: 93 530 205 1089 85 1071 3402 Fragments arranged by size: 3402 1089 1071 530 205 93 85 BsiWI C'GTAC_G Cuts at: 3087 3087 Size: 6475 BsmI GAATG_Cn' Cuts at: 2147 3986 4079 2147 Size: 1839 93 4543 Fragments arranged by size: 4543 1839 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2619 2712 2771 2858 2917 3043 3242 Size: 399 829 93 59 87 59 126 199 Cuts at: 3242 3447 3794 4536 4621 5692 1391 Size: 205 347 742 85 1071 2174 Fragments arranged by size: 2174 1071 829 742 399 347 205 199 126 93 87 85 59 59 BspEI T'CCGG_A Cuts at: 2619 6138 2619 Size: 3519 2956 BspHI T'CATG_A Cuts at: 2824 4364 6094 2824 Size: 1540 1730 3205 Fragments arranged by size: 3205 1730 1540 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 2323 3392 3519 3683 3830 844 Size: 1454 25 1069 127 164 147 3489 Fragments arranged by size: 3489 1454 1069 164 147 127 25 BsrBI CCG'CTC Cuts at: 1394 1823 4362 1394 Size: 429 2539 3507 Fragments arranged by size: 3507 2539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3282 3624 4970 5144 65 Size: 1183 2034 342 1346 174 1396 Fragments arranged by size: 2034 1396 1346 1183 342 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5119 5306 1700 Size: 110 3309 187 2869 Fragments arranged by size: 3309 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 4535 5547 4535 Size: 1012 5463 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6475 BstYI r'GATC_y Cuts at: 1972 3805 4172 4557 4574 6026 6112 6124 6470 Size: 1833 367 385 17 1452 86 12 346 Cuts at: 6470 1972 Size: 1977 Fragments arranged by size: 1977 1833 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2701 513 Size: 552 939 697 4287 Fragments arranged by size: 4287 939 697 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3673 487 Size: 683 56 2447 3289 Fragments arranged by size: 3289 2447 683 56 BtsI GCAGTG_nn' Cuts at: 3488 3745 3991 4785 4805 3488 Size: 257 246 794 20 5158 Fragments arranged by size: 5158 794 257 246 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3031 3779 3031 Size: 748 5727 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2936 5482 809 Size: 507 1620 2546 1802 Fragments arranged by size: 2546 1802 1620 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2086 2295 Size: 54 1425 50 275 18 228 28 209 Cuts at: 2295 3922 4811 6440 8 Size: 1627 889 1629 43 Fragments arranged by size: 1629 1627 1425 889 275 228 209 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 3922 1537 Size: 275 246 1864 4090 Fragments arranged by size: 4090 1864 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 2357 4405 1353 Size: 845 159 2048 3423 Fragments arranged by size: 3423 2048 845 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5062 5436 5582 387 Size: 1064 138 3473 374 146 1280 Fragments arranged by size: 3473 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2214 2222 2436 2514 3173 3308 Size: 393 495 87 8 214 78 659 135 Cuts at: 3308 3584 3695 4538 5135 5922 6190 1239 Size: 276 111 843 597 787 268 1524 Fragments arranged by size: 1524 843 787 659 597 495 393 276 268 214 135 111 87 78 8 EcoNI CCTnn'n_nnAGG Cuts at: 2872 2872 Size: 6475 EcoO109I rG'GnC_Cy Cuts at: 2473 2877 3213 3409 2473 Size: 404 336 196 5539 Fragments arranged by size: 5539 404 336 196 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2678 2678 Size: 6475 FspI TGC'GCA Cuts at: 2780 4981 2780 Size: 2201 4274 HaeII r_GCGC'y Cuts at: 1678 2028 2806 5622 1678 Size: 350 778 2816 2531 Fragments arranged by size: 2816 2531 778 350 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3120 3140 3152 3172 5116 Size: 32 135 32 1548 20 12 20 1944 Cuts at: 5116 5148 5190 5222 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1944 1548 135 42 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3819 4065 669 Size: 988 281 1221 660 246 3079 Fragments arranged by size: 3079 1221 988 660 281 246 HindIII A'AGCT_T Cuts at: 748 2534 3428 748 Size: 1786 894 3795 Fragments arranged by size: 3795 1786 894 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KasI G'GCGC_C Cuts at: 2024 2024 Size: 6475 KpnI G_GTAC'C Cuts at: 2557 3699 3800 2557 Size: 1142 101 5232 Fragments arranged by size: 5232 1142 101 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5589 5773 1305 Size: 4284 184 2007 Fragments arranged by size: 4284 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4653 Fragments arranged by size: 4653 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2573 2960 3611 3767 4433 4792 Size: 648 1104 303 387 651 156 666 359 Cuts at: 4792 4951 518 Size: 159 2042 Fragments arranged by size: 2042 1104 666 651 648 387 359 303 159 156 MspA1I CmG'CkG Cuts at: 1606 2270 2774 2861 3331 3418 3454 3891 4568 Size: 664 504 87 470 87 36 437 677 Cuts at: 4568 5716 5961 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 504 470 437 245 87 87 36 NarI GG'CG_CC Cuts at: 2025 2025 Size: 6475 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4984 Fragments arranged by size: 4984 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 2589 3833 5378 1132 Size: 97 1360 1244 1545 2229 Fragments arranged by size: 2229 1545 1360 1244 97 PasI CC'CwG_GG Cuts at: 1348 2874 1348 Size: 1526 4949 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 3082 1108 Size: 1974 4501 PfoI T'CCnGG_A Cuts at: 3003 3672 3003 Size: 669 5806 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PmlI CAC'GTG Cuts at: 2466 2466 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2473 3409 2473 Size: 936 5539 PshAI GACnn'nnGTC Cuts at: 1613 2832 1613 Size: 1219 5256 PsiI TTA'TAA Cuts at: 2484 4045 2484 Size: 1561 4914 PstI C_TGCA'G Cuts at: 830 3336 3417 3827 830 Size: 2506 81 410 3478 Fragments arranged by size: 3478 2506 410 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2861 3331 3418 3454 2861 Size: 470 87 36 5882 Fragments arranged by size: 5882 470 87 36 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 3332 3413 3823 4958 5639 5830 826 Size: 219 2287 81 410 1135 681 191 1471 Fragments arranged by size: 2287 1471 1135 681 410 219 191 81 SfoI GGC'GCC Cuts at: 2026 2026 Size: 6475 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 2165 3802 1872 Size: 293 1637 4545 Fragments arranged by size: 4545 1637 293 SmlI C'TyrA_G Cuts at: 820 1017 1943 2608 3473 4579 5480 5742 820 Size: 197 926 665 865 1106 901 262 1553 Fragments arranged by size: 1553 1106 926 901 865 665 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 3223 152 Size: 3071 3404 SphI G_CATG'C Cuts at: 1132 2589 3833 1132 Size: 1457 1244 3774 Fragments arranged by size: 3774 1457 1244 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6475 StyI C'CwwG_G Cuts at: 513 1065 2004 2850 3317 3914 513 Size: 552 939 846 467 597 3074 Fragments arranged by size: 3074 939 846 597 552 467 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3269 3283 4502 4519 4672 4857 5302 Size: 1255 1689 14 1219 17 153 185 445 Cuts at: 5302 325 Size: 1498 Fragments arranged by size: 1689 1498 1255 1219 445 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3529 4721 6433 Size: 274 80 33 51 1443 1552 1192 1712 Cuts at: 6433 96 Size: 138 Fragments arranged by size: 1712 1552 1443 1192 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6435 6467 6435 Size: 32 6443 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 2608 1943 Size: 665 5810 XmaI C'CCGG_G Cuts at: 1870 2163 3800 1870 Size: 293 1637 4545 Fragments arranged by size: 4545 1637 293 XmnI GAAnn'nnTTC Cuts at: 3180 4604 3180 Size: 1424 5051 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3622 4283 276 Size: 53 83 186 936 2088 661 2468 Fragments arranged by size: 2468 2088 936 661 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI