(Circular) (Six-base) MAPSORT of: pFN21AB7810.seq Check: 9083 from: 1 to: 6490 pFN21AB7810 6490 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2374 2374 Size: 6490 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2220 3833 1937 Size: 283 1613 4594 Fragments arranged by size: 4594 1613 283 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2565 2998 3422 4553 5937 6205 1632 Size: 933 433 424 1131 1384 268 1917 Fragments arranged by size: 1917 1384 1131 933 433 424 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 5389 6366 1203 Size: 4186 977 1327 Fragments arranged by size: 4186 1327 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 5219 5219 Size: 6490 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AlwNI CAG_nnn'CTG Cuts at: 3514 4233 5805 3514 Size: 719 1572 4199 Fragments arranged by size: 4199 1572 719 ApaLI G'TGCA_C Cuts at: 2683 4547 5703 2683 Size: 1864 1156 3470 Fragments arranged by size: 3470 1864 1156 ApoI r'AATT_y Cuts at: 1493 2461 3799 4025 4177 6286 1493 Size: 968 1338 226 152 2109 1697 Fragments arranged by size: 2109 1697 1338 968 226 152 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2168 2249 3815 1870 Size: 73 225 81 1566 4545 Fragments arranged by size: 4545 1566 225 81 73 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2386 3094 3604 3811 Size: 325 217 627 387 212 708 510 207 Cuts at: 3811 5266 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 708 627 510 387 325 217 212 207 BanII G_rGCy'C Cuts at: 1391 2079 2324 2412 2976 3722 3809 1391 Size: 688 245 88 564 746 87 4072 Fragments arranged by size: 4072 746 688 564 245 88 87 BbeI G_GCGC'C Cuts at: 2178 2178 Size: 6490 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5519 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3076 3110 4681 4715 1903 Size: 34 1139 34 1571 34 3678 Fragments arranged by size: 3678 1571 1139 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2760 4382 5592 2760 Size: 1622 1210 3658 Fragments arranged by size: 3658 1622 1210 BclI T'GATC_A Cuts at: 1548 2895 3075 3124 1548 Size: 1347 180 49 4914 Fragments arranged by size: 4914 1347 180 49 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2484 5101 136 Size: 107 122 71 631 605 812 2617 1525 Fragments arranged by size: 2617 1525 812 631 605 122 107 71 BglII A'GATC_T Cuts at: 3273 6485 3273 Size: 3212 3278 BlpI GC'TnA_GC Cuts at: 2970 3907 2970 Size: 937 5553 Bme1580I G_kGCm'C Cuts at: 1790 2391 2687 3609 4551 5707 1790 Size: 601 296 922 942 1156 2573 Fragments arranged by size: 2573 1156 942 922 601 296 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3091 4221 5179 Size: 416 53 83 399 24 1674 1130 958 Cuts at: 5179 442 Size: 1753 Fragments arranged by size: 1753 1674 1130 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2129 2420 2874 3393 3647 5150 1239 Size: 854 36 291 454 519 254 1503 2579 Fragments arranged by size: 2579 1503 854 519 454 291 254 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 4615 5480 5778 2081 Size: 2534 865 298 2793 Fragments arranged by size: 2793 2534 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3179 5153 882 Size: 617 1680 1974 2219 Fragments arranged by size: 2219 1974 1680 617 BsaAI yAC'GTr Cuts at: 493 1204 4281 493 Size: 711 3077 2702 Fragments arranged by size: 3077 2702 711 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 2476 3639 4297 Size: 53 83 186 936 642 301 1163 658 Cuts at: 4297 4679 5314 275 Size: 382 635 1451 Fragments arranged by size: 1451 1163 936 658 642 635 382 301 186 83 53 BsaWI w'CCGG_w Cuts at: 4923 5321 5595 5742 6153 4923 Size: 398 274 147 411 5260 Fragments arranged by size: 5260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3326 3356 3563 3593 1178 Size: 30 165 30 1923 30 207 30 4075 Fragments arranged by size: 4075 1923 207 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2799 3347 3515 3518 3701 1186 Size: 1613 548 168 3 183 3975 Fragments arranged by size: 3975 1613 548 183 168 3 BseYI C'CCAG_C Cuts at: 2411 3519 5693 6189 2411 Size: 1108 2174 496 2712 Fragments arranged by size: 2712 2174 1108 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2580 1163 Size: 573 585 259 5073 Fragments arranged by size: 5073 585 573 259 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2202 2940 3789 3940 4701 5729 Size: 275 186 201 738 849 151 761 1028 Cuts at: 5729 1540 Size: 2301 Fragments arranged by size: 2301 1028 849 761 738 275 201 186 151 BsiHKAI G_wGCw'C Cuts at: 2687 3198 4551 4636 5707 2687 Size: 511 1353 85 1071 3470 Fragments arranged by size: 3470 1353 1071 511 85 BsmI GAATG_Cn' Cuts at: 2447 3733 4001 4094 2447 Size: 1286 268 93 4843 Fragments arranged by size: 4843 1286 268 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3683 3683 Size: 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2324 2391 2412 2687 2976 3198 Size: 399 289 245 67 21 275 289 222 Cuts at: 3198 3609 3722 3809 4551 4636 5707 1391 Size: 411 113 87 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 289 289 275 245 222 113 87 85 67 21 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 2892 4379 6109 2892 Size: 1487 1730 3273 Fragments arranged by size: 3273 1730 1487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2206 2374 3627 3845 844 Size: 1362 168 1253 218 3489 Fragments arranged by size: 3489 1362 1253 218 168 BsrBI CCG'CTC Cuts at: 1394 1823 2113 2890 4377 1394 Size: 429 290 777 1487 3507 Fragments arranged by size: 3507 1487 777 429 290 BsrDI GCAATG_nn' Cuts at: 65 1248 2575 4985 5159 65 Size: 1183 1327 2410 174 1396 Fragments arranged by size: 2410 1396 1327 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2231 2435 2443 2724 5134 5321 1700 Size: 110 421 204 8 281 2410 187 2869 Fragments arranged by size: 2869 2410 421 281 204 187 110 8 BsrGI T'GTAC_A Cuts at: 96 2157 3378 3579 96 Size: 2061 1221 201 3007 Fragments arranged by size: 3007 2061 1221 201 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 4550 5562 4550 Size: 1012 5478 BstAPI GCAn_nnn'nTGC Cuts at: 794 3591 3739 794 Size: 2797 148 3545 Fragments arranged by size: 3545 2797 148 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstXI CCAn_nnnn'nTGG Cuts at: 2284 2284 Size: 6490 BstYI r'GATC_y Cuts at: 1972 2055 2100 3159 3273 3820 4187 4572 4589 Size: 83 45 1059 114 547 367 385 17 Cuts at: 4589 6041 6127 6139 6485 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1059 547 385 367 346 114 86 83 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2196 2374 3189 3667 3681 3783 513 Size: 552 1131 178 815 478 14 102 3220 Fragments arranged by size: 3220 1131 815 552 478 178 102 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3714 487 Size: 683 56 2488 3263 Fragments arranged by size: 3263 2488 683 56 BtsI GCAGTG_nn' Cuts at: 2568 3711 4006 4800 4820 2568 Size: 1143 295 794 20 4238 Fragments arranged by size: 4238 1143 794 295 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2948 5497 809 Size: 507 1632 2549 1802 Fragments arranged by size: 2549 1802 1632 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2377 2499 Size: 54 1425 50 275 18 369 178 122 Cuts at: 2499 2879 2937 3474 3708 3937 4826 6455 8 Size: 380 58 537 234 229 889 1629 43 Fragments arranged by size: 1629 1425 889 537 380 369 275 234 229 178 122 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2199 2937 3937 1537 Size: 275 387 738 1000 4090 Fragments arranged by size: 4090 1000 738 387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2702 2776 3083 3408 4420 1353 Size: 1349 74 307 325 1012 3423 Fragments arranged by size: 3423 1349 1012 325 307 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5077 5451 5597 387 Size: 1064 138 3488 374 146 1280 Fragments arranged by size: 3488 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2129 2420 2565 2874 2998 3393 Size: 393 461 36 291 145 309 124 395 Cuts at: 3393 3422 3647 4553 5150 5937 6205 1239 Size: 29 225 906 597 787 268 1524 Fragments arranged by size: 1524 906 787 597 461 395 393 309 291 268 225 145 124 36 29 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2123 Size: 6490 EcoRI G'AATT_C Cuts at: 3799 4177 3799 Size: 378 6112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2644 2644 Size: 6490 FseI GG_CCGG'CC Cuts at: 2439 2439 Size: 6490 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 2178 2812 3187 5637 1678 Size: 500 634 375 2450 2531 Fragments arranged by size: 2531 2450 634 500 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3157 3189 5131 5163 5205 Size: 32 135 32 1585 32 1942 32 42 Cuts at: 5205 5237 1373 Size: 32 2626 Fragments arranged by size: 2626 1942 1585 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 3021 748 Size: 2273 4217 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KasI G'GCGC_C Cuts at: 2174 2174 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 2943 5604 5788 1305 Size: 996 642 2661 184 2007 Fragments arranged by size: 2661 2007 996 642 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2501 2881 3476 3710 10 Size: 54 1425 343 669 380 595 234 2790 Fragments arranged by size: 2790 1425 669 595 380 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4448 4807 4966 518 Size: 648 843 2439 359 159 2042 Fragments arranged by size: 2439 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2198 2326 2353 2415 3511 3906 4583 5731 Size: 592 128 27 62 1096 395 677 1148 Cuts at: 5731 5976 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1096 677 592 395 245 128 62 27 NaeI GCC'GGC Cuts at: 2437 2445 2726 2437 Size: 8 281 6201 Fragments arranged by size: 6201 281 8 NarI GG'CG_CC Cuts at: 2175 2175 Size: 6490 NcoI C'CATG_G Cuts at: 513 1065 3189 513 Size: 552 2124 3814 Fragments arranged by size: 3814 2124 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NgoMIV G'CCGG_C Cuts at: 2435 2443 2724 2435 Size: 8 281 6201 Fragments arranged by size: 6201 281 8 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 2199 3937 2199 Size: 1738 4752 NspI r_CATG'y Cuts at: 1132 1229 3286 3656 3848 5393 1132 Size: 97 2057 370 192 1545 2229 Fragments arranged by size: 2229 2057 1545 370 192 97 PasI CC'CwG_GG Cuts at: 1348 2119 3200 1348 Size: 771 1081 4638 Fragments arranged by size: 4638 1081 771 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 2877 3472 3514 1108 Size: 1769 595 42 4084 Fragments arranged by size: 4084 1769 595 42 PfoI T'CCnGG_A Cuts at: 2033 3438 2033 Size: 1405 5085 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4559 4591 6072 6104 4559 Size: 32 1481 32 4945 Fragments arranged by size: 4945 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PstI C_TGCA'G Cuts at: 830 2370 3327 3498 3842 830 Size: 1540 957 171 344 3478 Fragments arranged by size: 3478 1540 957 344 171 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 2353 2353 Size: 6490 SacII CC_GC'GG Cuts at: 2199 2199 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SapI GCTCTTCn'nnn_ Cuts at: 2702 2702 Size: 6490 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SfcI C'TryA_G Cuts at: 826 1045 2366 2452 3323 3494 3838 4973 5654 Size: 219 1321 86 871 171 344 1135 681 Cuts at: 5654 5845 826 Size: 191 1471 Fragments arranged by size: 1471 1321 1135 871 681 344 219 191 171 86 SfoI GGC'GCC Cuts at: 2176 2176 Size: 6490 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 2251 3817 1872 Size: 379 1566 4545 Fragments arranged by size: 4545 1566 379 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 4594 5495 5757 820 Size: 197 926 153 2498 901 262 1553 Fragments arranged by size: 2498 1553 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 2624 2720 3486 1517 Size: 1107 96 766 4521 Fragments arranged by size: 4521 1107 766 96 StyI C'CwwG_G Cuts at: 513 1065 2059 2541 3189 3477 3929 513 Size: 552 994 482 648 288 452 3074 Fragments arranged by size: 3074 994 648 552 482 452 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2793 3213 4517 4534 4687 4872 5317 Size: 1255 1213 420 1304 17 153 185 445 Cuts at: 5317 325 Size: 1498 Fragments arranged by size: 1498 1304 1255 1213 445 420 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 3378 3579 Size: 274 80 33 51 1443 180 1221 201 Cuts at: 3579 4736 6448 96 Size: 1157 1712 138 Fragments arranged by size: 1712 1443 1221 1157 274 201 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 6450 6482 3326 Size: 32 3092 32 3334 Fragments arranged by size: 3334 3092 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3139 3826 3139 Size: 687 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2819 2819 Size: 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 2249 3815 1870 Size: 379 1566 4545 Fragments arranged by size: 4545 1566 379 XmnI GAAnn'nnTTC Cuts at: 4619 4619 Size: 6490 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI