Restriction map for
pFN21AB7810
(Linear) (Six-base) MAPSORT of: KIBB7810.seq Check: 4142 from: 1 to: 1785
KIBB7810.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 369 1785
Size: 369 1416
AccI GT'mk_AC
Cuts at: 0 215 1785
Size: 215 1570
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 993 1417 1785
Size: 560 433 424 368
Fragments arranged by size:
560 433 424 368
AlwNI CAG_nnn'CTG
Cuts at: 0 1509 1785
Size: 1509 276
ApaLI G'TGCA_C
Cuts at: 0 678 1785
Size: 678 1107
ApoI r'AATT_y
Cuts at: 0 456 1785
Size: 456 1329
AvaI C'yCGr_G
Cuts at: 0 163 244 1785
Size: 163 81 1541
Fragments arranged by size:
1541 163 81
BanI G'GyrC_C
Cuts at: 0 169 381 1089 1599 1785
Size: 169 212 708 510 186
Fragments arranged by size:
708 510 212 186 169
BanII G_rGCy'C
Cuts at: 0 74 319 407 971 1717 1785
Size: 74 245 88 564 746 68
Fragments arranged by size:
746 564 245 88 74 68
BbeI G_GCGC'C
Cuts at: 0 173 1785
Size: 173 1612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1071 1105 1785
Size: 1071 34 680
Fragments arranged by size:
1071 680 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 755 1785
Size: 755 1030
BclI T'GATC_A
Cuts at: 0 890 1070 1119 1785
Size: 890 180 49 666
Fragments arranged by size:
890 666 180 49
BglI GCCn_nnn'nGGC
Cuts at: 0 479 1785
Size: 479 1306
BglII A'GATC_T
Cuts at: 0 1268 1785
Size: 1268 517
BlpI GC'TnA_GC
Cuts at: 0 965 1785
Size: 965 820
Bme1580I G_kGCm'C
Cuts at: 0 386 682 1604 1785
Size: 386 296 922 181
Fragments arranged by size:
922 386 296 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 1086 1785
Size: 1086 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 124 415 869 1388 1642 1785
Size: 88 36 291 454 519 254 143
Fragments arranged by size:
519 454 291 254 143 88 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1785
Size: 76 1709
BsaI GGTCTCn'nnnn_
Cuts at: 0 1174 1785
Size: 1174 611
BsaHI Gr'CG_yC
Cuts at: 0 170 471 1634 1785
Size: 170 301 1163 151
Fragments arranged by size:
1163 301 170 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1321 1351 1558 1588 1785
Size: 1321 30 207 30 197
Fragments arranged by size:
1321 207 197 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 794 1342 1510 1513 1696 1785
Size: 794 548 168 3 183 89
Fragments arranged by size:
794 548 183 168 89 3
BseYI C'CCAG_C
Cuts at: 0 406 1514 1785
Size: 406 1108 271
Fragments arranged by size:
1108 406 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 575 1785
Size: 316 259 1210
Fragments arranged by size:
1210 316 259
BsiEI CG_ry'CG
Cuts at: 0 197 935 1785
Size: 197 738 850
Fragments arranged by size:
850 738 197
BsiHKAI G_wGCw'C
Cuts at: 0 682 1193 1785
Size: 682 511 592
Fragments arranged by size:
682 592 511
BsmI GAATG_Cn'
Cuts at: 0 442 1728 1785
Size: 442 1286 57
Fragments arranged by size:
1286 442 57
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1678 1785
Size: 1678 107
Bsp1286I G_dGCh'C
Cuts at: 0 74 319 386 407 682 971 1193 1604
Size: 74 245 67 21 275 289 222 411
Cuts at: 1604 1717 1785
Size: 113 68
Fragments arranged by size:
411 289 275 245 222 113 74 68
67 21
BspHI T'CATG_A
Cuts at: 0 887 1785
Size: 887 898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 369 1622 1785
Size: 201 168 1253 163
Fragments arranged by size:
1253 201 168 163
BsrBI CCG'CTC
Cuts at: 0 108 885 1785
Size: 108 777 900
Fragments arranged by size:
900 777 108
BsrDI GCAATG_nn'
Cuts at: 0 570 1785
Size: 570 1215
BsrFI r'CCGG_y
Cuts at: 0 226 430 438 719 1785
Size: 226 204 8 281 1066
Fragments arranged by size:
1066 281 226 204 8
BsrGI T'GTAC_A
Cuts at: 0 152 1373 1574 1785
Size: 152 1221 201 211
Fragments arranged by size:
1221 211 201 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1586 1734 1785
Size: 1586 148 51
Fragments arranged by size:
1586 148 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 1785
Size: 279 1506
BstYI r'GATC_y
Cuts at: 0 50 95 1154 1268 1785
Size: 50 45 1059 114 517
Fragments arranged by size:
1059 517 114 50 45
BtgI C'CryG_G
Cuts at: 0 191 369 1184 1662 1676 1778 1785
Size: 191 178 815 478 14 102 7
Fragments arranged by size:
815 478 191 178 102 14 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1709 1785
Size: 1709 76
BtsI GCAGTG_nn'
Cuts at: 0 563 1706 1785
Size: 563 1143 79
Fragments arranged by size:
1143 563 79
DrdI GACnn_nn'nnGTC
Cuts at: 0 943 1785
Size: 943 842
EaeI y'GGCC_r
Cuts at: 0 194 372 494 874 932 1469 1703 1785
Size: 194 178 122 380 58 537 234 82
Fragments arranged by size:
537 380 234 194 178 122 82 58
EagI C'GGCC_G
Cuts at: 0 194 932 1785
Size: 194 738 853
Fragments arranged by size:
853 738 194
EarI CTCTTCn'nnn_
Cuts at: 0 697 771 1078 1403 1785
Size: 697 74 307 325 382
Fragments arranged by size:
697 382 325 307 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 124 415 560 869 993 1388 1417
Size: 88 36 291 145 309 124 395 29
Cuts at: 1417 1642 1785
Size: 225 143
Fragments arranged by size:
395 309 291 225 145 143 124 88
36 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 1785
Size: 118 1667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 639 1785
Size: 639 1146
FseI GG_CCGG'CC
Cuts at: 0 434 1785
Size: 434 1351
HaeII r_GCGC'y
Cuts at: 0 173 807 1182 1785
Size: 173 634 375 603
Fragments arranged by size:
634 603 375 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1152 1184 1785
Size: 1152 32 601
Fragments arranged by size:
1152 601 32
HindIII A'AGCT_T
Cuts at: 0 1016 1785
Size: 1016 769
KasI G'GCGC_C
Cuts at: 0 169 1785
Size: 169 1616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 938 1785
Size: 296 642 847
Fragments arranged by size:
847 642 296
MscI TGG'CCA
Cuts at: 0 496 876 1471 1705 1785
Size: 496 380 595 234 80
Fragments arranged by size:
595 496 380 234 80
MspA1I CmG'CkG
Cuts at: 0 193 321 348 410 1506 1785
Size: 193 128 27 62 1096 279
Fragments arranged by size:
1096 279 193 128 62 27
NaeI GCC'GGC
Cuts at: 0 432 440 721 1785
Size: 432 8 281 1064
Fragments arranged by size:
1064 432 281 8
NarI GG'CG_CC
Cuts at: 0 170 1785
Size: 170 1615
NcoI C'CATG_G
Cuts at: 0 1184 1785
Size: 1184 601
NgoMIV G'CCGG_C
Cuts at: 0 430 438 719 1785
Size: 430 8 281 1066
Fragments arranged by size:
1066 430 281 8
NotI GC'GGCC_GC
Cuts at: 0 194 1785
Size: 194 1591
NspI r_CATG'y
Cuts at: 0 1281 1651 1785
Size: 1281 370 134
Fragments arranged by size:
1281 370 134
PasI CC'CwG_GG
Cuts at: 0 114 1195 1785
Size: 114 1081 590
Fragments arranged by size:
1081 590 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 872 1467 1509 1785
Size: 872 595 42 276
Fragments arranged by size:
872 595 276 42
PfoI T'CCnGG_A
Cuts at: 0 28 1433 1785
Size: 28 1405 352
Fragments arranged by size:
1405 352 28
PstI C_TGCA'G
Cuts at: 0 365 1322 1493 1785
Size: 365 957 171 292
Fragments arranged by size:
957 365 292 171
PvuII CAG'CTG
Cuts at: 0 348 1785
Size: 348 1437
SacII CC_GC'GG
Cuts at: 0 194 1785
Size: 194 1591
SapI GCTCTTCn'nnn_
Cuts at: 0 697 1785
Size: 697 1088
SfcI C'TryA_G
Cuts at: 0 361 447 1318 1489 1785
Size: 361 86 871 171 296
Fragments arranged by size:
871 361 296 171 86
SfoI GGC'GCC
Cuts at: 0 171 1785
Size: 171 1614
SmaI CCC'GGG
Cuts at: 0 246 1785
Size: 246 1539
SmlI C'TyrA_G
Cuts at: 0 91 1785
Size: 91 1694
StuI AGG'CCT
Cuts at: 0 619 715 1481 1785
Size: 619 96 766 304
Fragments arranged by size:
766 619 304 96
StyI C'CwwG_G
Cuts at: 0 54 536 1184 1472 1785
Size: 54 482 648 288 313
Fragments arranged by size:
648 482 313 288 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 788 1208 1785
Size: 788 420 577
Fragments arranged by size:
788 577 420
TatI w'GTAC_w
Cuts at: 0 152 1373 1574 1785
Size: 152 1221 201 211
Fragments arranged by size:
1221 211 201 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 1785
Size: 1321 32 432
Fragments arranged by size:
1321 432 32
XbaI T'CTAG_A
Cuts at: 0 1134 1785
Size: 1134 651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 814 1785
Size: 814 971
XmaI C'CCGG_G
Cuts at: 0 244 1785
Size: 244 1541
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaLI ApoI AvaI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EagI EarI Eco57MI
EcoNI FalI FseI HaeII Hin4I HindIII KasI MmeI
MscI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PflMI PfoI PstI PvuII SacII SapI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BfrBI BmgBI BmtI BplI
Bpu10I BsaAI BsaBI BsaWI BsiWI BspEI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
EciI EcoICRI EcoO109I EcoRI EcoRV FspI FspAI HincII
HpaI KpnI MfeI MluI MslI NdeI NheI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XhoI XmnI
ZraI