Restriction map for
pFN21AB6943
(Linear) (Six-base) MAPSORT of: KIBB6943.seq Check: 9196 from: 1 to: 549
KIBB6943.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AfeI AGC'GCT
Cuts at: 0 26 549
Size: 26 523
AflIII A'CryG_T
Cuts at: 0 299 549
Size: 299 250
AvaI C'yCGr_G
Cuts at: 0 158 213 549
Size: 158 55 336
Fragments arranged by size:
336 158 55
BanI G'GyrC_C
Cuts at: 0 165 549
Size: 165 384
BanII G_rGCy'C
Cuts at: 0 475 549
Size: 475 74
BbeI G_GCGC'C
Cuts at: 0 169 549
Size: 169 380
BbsI GAAGACnn'nnnn_
Cuts at: 0 499 531 549
Size: 499 32 18
Fragments arranged by size:
499 32 18
BglI GCCn_nnn'nGGC
Cuts at: 0 163 549
Size: 163 386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 435 549
Size: 435 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 145 374 380 549
Size: 106 39 229 6 169
Fragments arranged by size:
229 169 106 39 6
BsaHI Gr'CG_yC
Cuts at: 0 166 549
Size: 166 383
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 549
Size: 114 435
BseYI C'CCAG_C
Cuts at: 0 401 549
Size: 401 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 549
Size: 280 269
BsiEI CG_ry'CG
Cuts at: 0 20 549
Size: 20 529
Bsp1286I G_dGCh'C
Cuts at: 0 475 549
Size: 475 74
BsrFI r'CCGG_y
Cuts at: 0 186 193 549
Size: 186 7 356
Fragments arranged by size:
356 186 7
EaeI y'GGCC_r
Cuts at: 0 17 549
Size: 17 532
EagI C'GGCC_G
Cuts at: 0 17 549
Size: 17 532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 145 374 380 549
Size: 106 39 229 6 169
Fragments arranged by size:
229 169 106 39 6
EcoO109I rG'GnC_Cy
Cuts at: 0 150 426 452 549
Size: 150 276 26 97
Fragments arranged by size:
276 150 97 26
HaeII r_GCGC'y
Cuts at: 0 28 169 549
Size: 28 141 380
Fragments arranged by size:
380 141 28
KasI G'GCGC_C
Cuts at: 0 165 549
Size: 165 384
NaeI GCC'GGC
Cuts at: 0 188 195 549
Size: 188 7 354
Fragments arranged by size:
354 188 7
NarI GG'CG_CC
Cuts at: 0 166 549
Size: 166 383
NgoMIV G'CCGG_C
Cuts at: 0 186 193 549
Size: 186 7 356
Fragments arranged by size:
356 186 7
NotI GC'GGCC_GC
Cuts at: 0 17 549
Size: 17 532
NspI r_CATG'y
Cuts at: 0 121 303 388 549
Size: 121 182 85 161
Fragments arranged by size:
182 161 121 85
PciI A'CATG_T
Cuts at: 0 299 549
Size: 299 250
PfoI T'CCnGG_A
Cuts at: 0 357 549
Size: 357 192
PpuMI rG'GwC_Cy
Cuts at: 0 150 426 452 549
Size: 150 276 26 97
Fragments arranged by size:
276 150 97 26
PstI C_TGCA'G
Cuts at: 0 56 549
Size: 56 493
SexAI A'CCwGG_T
Cuts at: 0 533 549
Size: 533 16
SfcI C'TryA_G
Cuts at: 0 52 549
Size: 52 497
SfoI GGC'GCC
Cuts at: 0 167 549
Size: 167 382
SmaI CCC'GGG
Cuts at: 0 160 215 549
Size: 160 55 334
Fragments arranged by size:
334 160 55
SphI G_CATG'C
Cuts at: 0 121 388 549
Size: 121 267 161
Fragments arranged by size:
267 161 121
SspI AAT'ATT
Cuts at: 0 226 549
Size: 226 323
StyI C'CwwG_G
Cuts at: 0 351 549
Size: 351 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 56 549
Size: 56 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 512 544 549
Size: 512 32 5
Fragments arranged by size:
512 32 5
Tth111I GACn'n_nGTC
Cuts at: 0 535 549
Size: 535 14
XbaI T'CTAG_A
Cuts at: 0 75 549
Size: 75 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 549
Size: 141 408
XmaI C'CCGG_G
Cuts at: 0 158 213 549
Size: 158 55 336
Fragments arranged by size:
336 158 55
Enzymes that do cut:
AfeI AflIII AvaI BanI BanII BbeI BbsI BglI
BplI BpmI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I
BsrFI EaeI EagI Eco57MI EcoO109I HaeII KasI NaeI
NarI NgoMIV NotI NspI PciI PfoI PpuMI PstI
SexAI SfcI SfoI SmaI SphI SspI StyI TaqII
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI StuI SwaI TatI XhoI XmnI ZraI