Restriction map for
pFN21AB6929
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Aor51HI ApoI
AscI AseI AsnI Asp700I AvaII
AvrII BalI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI EarI Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp509I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 492 GGTACC G^GTACC
AhdI 222 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 376 GTGCAC G^TGCAC
AlwNI 612 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 31 CCTGAGG CC^T[ACGT]AGG
ApaLI 376 GTGCAC G^TGCAC
Asp718I 492 GGTACC G^GTACC
AspEI 222 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 130 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 602 TGCGCA TGC^GCA
BamHI 56 GGATCC G^GATCC
BbeI 83 GGCGCC GGCGC^C
BseRI 256 GAGGAG GAGGAG(10/8)
BsiEI 452 CGGCCG CG[GA][CT]^CG
BsmAI 306 GAGAC GTCTC(1/5)
BsrFI 450 GCCGGC [GA]^CCGG[CT]
BsrI 101 CCAGT ACTGG(1/-1)
BstXI 539 CCATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 31 CCTGAGG CC^T[ACGT]AGG
Cfr10I 450 GCCGGC [GA]^CCGG[CT]
DdeI 32 CTGAG C^T[ACGT]AG
DraIII 520 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 452 CGGCCG C^GGCCG
Eam1105I 222 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 452 CGGCCG C^GGCCG
Eco52I 452 CGGCCG C^GGCCG
Eco81I 31 CCTGAGG CC^T[ACGT]AGG
FauI 585 GCGGG CCCGC(4/6)
FspI 602 TGCGCA TGC^GCA
KasI 83 GGCGCC G^GCGCC
KpnI 492 GGTACC GGTAC^C
MaeII 42 ACGT A^CGT
MaeIII 321 GTCAC ^GT[ACGT]AC
NaeI 450 GCCGGC GCC^GGC
NarI 83 GGCGCC GG^CGCC
NgoMIV 450 GCCGGC G^CCGGC
NspI 363 GCATGT [GA]CATG^[CT]
PflFI 130 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 519 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 568 GAGCTC GAGCT^C
SfoI 83 GGCGCC GGC^GCC
Tsp45I 321 GTCAC ^GT[GC]AC
Tth111I 130 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 519 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 83 GGCGCC G[GA]^CG[CT]C
181 GGCGTC
AfaI 195 GTAC GT^AC
493 GTAC
ApaI 405 GGGCCC GGGCC^C
528 GGGCCC
AvaI 61 CCCGAG C^[CT]CG[GA]G
69 CCCGAG
BanI 83 GGCGCC G^G[CT][GA]CC
492 GGTACC
BcnI 123 CCCGG CC^[GC]GG
462 CCGGG
BsaHI 83 GGCGCC G[GA]^CG[CT]C
181 GGCGTC
BsgI 331 CTGCAC GTGCAG(16/14)
430 CTGCAC
BsoBI 61 CCCGAG C^[CT]CG[GA]G
69 CCCGAG
BspMI 359 ACCTGC ACCTGC(4/8)
428 ACCTGC
BstF5I 411 CATCC GGATG(2/0)
201 GGATG
BstYI 56 GGATCC [GA]^GATC[CT]
206 AGATCC
DsaI 524 CCGTGG C^C[GA][CT]GG
545 CCGTGG
EaeI 371 TGGCCG [CT]^GGCC[GA]
452 CGGCCG
FokI 411 CATCC GGATG(9/13)
201 GGATG
Hin1I 83 GGCGCC G[GA]^CG[CT]C
181 GGCGTC
MflI 56 GGATCC [GA]^GATC[CT]
206 AGATCC
MspA1I 79 CAGCGG C[AC]G^C[GT]G
612 CAGCGG
NciI 123 CCCGG CC^[GC]GG
462 CCGGG
RsaI 195 GTAC GT^AC
493 GTAC
TaqI 152 TCGA T^CGA
254 TCGA
TthHB8I 152 TCGA T^CGA
254 TCGA
XhoII 56 GGATCC [GA]^GATC[CT]
206 AGATCC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII Fnu4HI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MnlI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TseI
TspRI