Restriction map for
pFN21AB6929
(Linear) (Six-base) MAPSORT of: KIBB6929.seq Check: 462 from: 1 to: 621
KIBB6929.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 368 621
Size: 368 253
Acc65I G'GTAC_C
Cuts at: 0 492 621
Size: 492 129
AhdI GACnn_n'nnGTC
Cuts at: 0 227 621
Size: 227 394
AlwNI CAG_nnn'CTG
Cuts at: 0 617 621
Size: 617 4
ApaI G_GGCC'C
Cuts at: 0 409 532 621
Size: 409 123 89
Fragments arranged by size:
409 123 89
ApaLI G'TGCA_C
Cuts at: 0 376 621
Size: 376 245
AvaI C'yCGr_G
Cuts at: 0 61 69 621
Size: 61 8 552
Fragments arranged by size:
552 61 8
BamHI G'GATC_C
Cuts at: 0 56 621
Size: 56 565
BanI G'GyrC_C
Cuts at: 0 83 492 621
Size: 83 409 129
Fragments arranged by size:
409 129 83
BanII G_rGCy'C
Cuts at: 0 409 532 553 572 621
Size: 409 123 21 19 49
Fragments arranged by size:
409 123 49 21 19
BbeI G_GCGC'C
Cuts at: 0 87 621
Size: 87 534
Bme1580I G_kGCm'C
Cuts at: 0 380 409 532 621
Size: 380 29 123 89
Fragments arranged by size:
380 123 89 29
BmgBI CAC'GTC
Cuts at: 0 43 621
Size: 43 578
BsaHI Gr'CG_yC
Cuts at: 0 84 182 621
Size: 84 98 439
Fragments arranged by size:
439 98 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 621
Size: 271 350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 415 621
Size: 316 99 206
Fragments arranged by size:
316 206 99
BsiEI CG_ry'CG
Cuts at: 0 455 621
Size: 455 166
BsiHKAI G_wGCw'C
Cuts at: 0 16 380 572 621
Size: 16 364 192 49
Fragments arranged by size:
364 192 49 16
Bsp1286I G_dGCh'C
Cuts at: 0 16 380 409 532 553 572 621
Size: 16 364 29 123 21 19 49
Fragments arranged by size:
364 123 49 29 21 19 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 368 437 621
Size: 368 69 184
Fragments arranged by size:
368 184 69
BsrFI r'CCGG_y
Cuts at: 0 450 621
Size: 450 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 546 621
Size: 546 75
BstYI r'GATC_y
Cuts at: 0 56 206 621
Size: 56 150 415
Fragments arranged by size:
415 150 56
Bsu36I CC'TnA_GG
Cuts at: 0 32 621
Size: 32 589
BtgI C'CryG_G
Cuts at: 0 524 545 621
Size: 524 21 76
Fragments arranged by size:
524 76 21
BtsI GCAGTG_nn'
Cuts at: 0 573 621
Size: 573 48
DraIII CAC_nnn'GTG
Cuts at: 0 525 621
Size: 525 96
EaeI y'GGCC_r
Cuts at: 0 371 452 621
Size: 371 81 169
Fragments arranged by size:
371 169 81
EagI C'GGCC_G
Cuts at: 0 452 621
Size: 452 169
EcoICRI GAG'CTC
Cuts at: 0 570 621
Size: 570 51
EcoO109I rG'GnC_Cy
Cuts at: 0 36 405 406 529 621
Size: 36 369 1 123 92
Fragments arranged by size:
369 123 92 36 1
FspI TGC'GCA
Cuts at: 0 604 621
Size: 604 17
HaeII r_GCGC'y
Cuts at: 0 87 143 426 621
Size: 87 56 283 195
Fragments arranged by size:
283 195 87 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 213 245 621
Size: 213 32 376
Fragments arranged by size:
376 213 32
KasI G'GCGC_C
Cuts at: 0 83 621
Size: 83 538
KpnI G_GTAC'C
Cuts at: 0 496 621
Size: 496 125
MslI CAynn'nnrTG
Cuts at: 0 362 544 562 621
Size: 362 182 18 59
Fragments arranged by size:
362 182 59 18
MspA1I CmG'CkG
Cuts at: 0 81 614 621
Size: 81 533 7
Fragments arranged by size:
533 81 7
NaeI GCC'GGC
Cuts at: 0 452 621
Size: 452 169
NarI GG'CG_CC
Cuts at: 0 84 621
Size: 84 537
NgoMIV G'CCGG_C
Cuts at: 0 450 621
Size: 450 171
NspI r_CATG'y
Cuts at: 0 367 621
Size: 367 254
PasI CC'CwG_GG
Cuts at: 0 243 621
Size: 243 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 525 621
Size: 525 96
PspOMI G'GGCC_C
Cuts at: 0 405 528 621
Size: 405 123 93
Fragments arranged by size:
405 123 93
SacI G_AGCT'C
Cuts at: 0 572 621
Size: 572 49
SfoI GGC'GCC
Cuts at: 0 85 621
Size: 85 536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 621
Size: 31 590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 41 73 621
Size: 41 32 548
Fragments arranged by size:
548 41 32
Tth111I GACn'n_nGTC
Cuts at: 0 133 621
Size: 133 488
Enzymes that do cut:
AarI Acc65I AhdI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI Bme1580I BmgBI BsaHI BseRI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EcoICRI EcoO109I FspI
HaeII Hin4I KasI KpnI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PspOMI SacI SfoI TaqII
TstI Tth111I
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI MfeI MluI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI XbaI XcmI XhoI XmaI
XmnI ZraI