(Circular) (Six-base) MAPSORT of: pFN21AB6902.seq Check: 8598 from: 1 to: 5374 pFN21AB6902 5374 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2269 2269 Size: 5374 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2073 3184 278 Size: 53 83 186 936 537 1111 2468 Fragments arranged by size: 2468 1111 936 537 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2558 1184 Size: 1374 4000 AlwNI CAG_nnn'CTG Cuts at: 2080 2521 3117 4689 2080 Size: 441 596 1572 2765 Fragments arranged by size: 2765 1572 596 441 ApaI G_GGCC'C Cuts at: 2399 2399 Size: 5374 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2699 1870 Size: 73 388 368 4545 Fragments arranged by size: 4545 388 368 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2695 4150 618 Size: 325 217 627 264 644 1455 1842 Fragments arranged by size: 1842 1455 644 627 325 264 217 BanII G_rGCy'C Cuts at: 1391 2399 2693 1391 Size: 1008 294 4072 Fragments arranged by size: 4072 1008 294 BbeI G_GCGC'C Cuts at: 2055 2055 Size: 5374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4403 BbvCI CC'TCA_GC Cuts at: 2343 2343 Size: 5374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3565 3599 1903 Size: 34 1628 34 3678 Fragments arranged by size: 3678 1628 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 2257 1548 Size: 709 4665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2148 3985 136 Size: 107 122 71 631 605 476 1837 1525 Fragments arranged by size: 1837 1525 631 605 476 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2399 3435 4591 1790 Size: 609 1036 1156 2573 Fragments arranged by size: 2573 1156 1036 609 BmgBI CAC'GTC Cuts at: 1318 2131 2203 1318 Size: 813 72 4489 Fragments arranged by size: 4489 813 72 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2384 3105 4063 Size: 416 53 83 399 24 967 721 958 Cuts at: 4063 442 Size: 1753 Fragments arranged by size: 1753 967 958 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4034 1239 Size: 2795 2579 Bpu10I CC'TnA_GC Cuts at: 2343 2343 Size: 5374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 2070 3181 3563 Size: 53 83 186 936 519 18 1111 382 Cuts at: 3563 4198 275 Size: 635 1451 Fragments arranged by size: 1451 1111 936 635 519 382 186 83 53 18 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5149 Fragments arranged by size: 5149 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2111 2300 1186 Size: 843 82 189 4260 Fragments arranged by size: 4260 843 189 82 BseYI C'CCAG_C Cuts at: 2443 2625 2644 4577 5073 2443 Size: 182 19 1933 496 2744 Fragments arranged by size: 2744 1933 496 182 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 2288 2564 1163 Size: 573 543 9 276 3973 Fragments arranged by size: 3973 573 543 276 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 3520 4591 3435 Size: 85 1071 4218 Fragments arranged by size: 4218 1071 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2399 2693 3435 3520 4591 1391 Size: 399 609 294 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 609 399 294 85 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2269 2729 844 Size: 1425 460 3489 Fragments arranged by size: 3489 1425 460 BsrBI CCG'CTC Cuts at: 1394 1823 2031 3261 1394 Size: 429 208 1230 3507 Fragments arranged by size: 3507 1230 429 208 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 4018 4205 1700 Size: 110 238 1970 187 2869 Fragments arranged by size: 2869 1970 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 3434 4446 3434 Size: 1012 4362 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 794 Size: 1508 3866 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstXI CCAn_nnnn'nTGG Cuts at: 2625 2625 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2402 513 Size: 552 1337 3485 Fragments arranged by size: 3485 1337 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2437 487 Size: 683 56 1211 3424 Fragments arranged by size: 3424 1211 683 56 BtsI GCAGTG_nn' Cuts at: 2274 2519 2542 2890 3684 3704 2274 Size: 245 23 348 794 20 3944 Fragments arranged by size: 3944 794 348 245 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3710 5339 Size: 54 1425 50 275 18 991 889 1629 Cuts at: 5339 8 Size: 43 Fragments arranged by size: 1629 1425 991 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3304 1353 Size: 1951 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3437 4034 4821 5089 1239 Size: 393 1805 597 787 268 1524 Fragments arranged by size: 1805 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2290 2395 2396 2290 Size: 105 1 5268 Fragments arranged by size: 5268 105 1 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2055 4521 1678 Size: 377 2466 2531 Fragments arranged by size: 2531 2466 377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373 Size: 32 135 32 2443 32 42 32 2626 Fragments arranged by size: 2626 2443 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KasI G'GCGC_C Cuts at: 2051 2051 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 2642 4488 4672 1305 Size: 729 608 1846 184 2007 Fragments arranged by size: 2007 1846 729 608 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3552 Fragments arranged by size: 3552 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 3332 3691 3850 518 Size: 648 990 1176 359 159 2042 Fragments arranged by size: 2042 1176 990 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 2302 2790 3467 4615 4860 1606 Size: 564 132 488 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 564 488 245 132 NaeI GCC'GGC Cuts at: 2050 2050 Size: 5374 NarI GG'CG_CC Cuts at: 2052 2052 Size: 5374 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 2243 387 Size: 1856 3518 NgoMIV G'CCGG_C Cuts at: 2048 2048 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NruI TCG'CGA Cuts at: 2313 2313 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PfoI T'CCnGG_A Cuts at: 2073 2073 Size: 5374 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PspOMI G'GGCC_C Cuts at: 2395 2395 Size: 5374 PstI C_TGCA'G Cuts at: 830 2253 2726 830 Size: 1423 473 3478 Fragments arranged by size: 3478 1423 473 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 PvuII CAG'CTG Cuts at: 2302 2302 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2249 2722 3857 4538 4729 826 Size: 219 1204 473 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 681 473 219 191 SfoI GGC'GCC Cuts at: 2053 2053 Size: 5374 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2333 2701 1872 Size: 461 368 4545 Fragments arranged by size: 4545 461 368 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2813 513 Size: 552 1748 3074 Fragments arranged by size: 3074 1748 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2519 3401 3418 3571 3756 4201 325 Size: 1255 939 882 17 153 185 445 1498 Fragments arranged by size: 1498 1255 939 882 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 5332 96 Size: 274 80 33 51 1443 1643 1712 138 Fragments arranged by size: 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5334 5366 5334 Size: 32 5342 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2150 2150 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2331 2699 1870 Size: 461 368 4545 Fragments arranged by size: 4545 461 368 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2071 3182 276 Size: 53 83 186 936 537 1111 2468 Fragments arranged by size: 2468 1111 936 537 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI