Restriction map for
pFN21AB6901
(Linear) (Six-base) MAPSORT of: KIBB6901.seq Check: 5327 from: 1 to: 609
KIBB6901.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 0 215 609
Size: 215 394
AclI AA'CG_TT
Cuts at: 0 459 609
Size: 459 150
ApoI r'AATT_y
Cuts at: 0 239 609
Size: 239 370
BanI G'GyrC_C
Cuts at: 0 93 609
Size: 93 516
BanII G_rGCy'C
Cuts at: 0 55 609
Size: 55 554
BbeI G_GCGC'C
Cuts at: 0 97 609
Size: 97 512
BbsI GAAGACnn'nnnn_
Cuts at: 0 291 609
Size: 291 318
BclI T'GATC_A
Cuts at: 0 561 609
Size: 561 48
BfrBI ATG'CAT
Cuts at: 0 349 609
Size: 349 260
BmtI G_CTAG'C
Cuts at: 0 599 609
Size: 599 10
BsaHI Gr'CG_yC
Cuts at: 0 58 94 212 609
Size: 58 36 118 397
Fragments arranged by size:
397 118 58 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 609
Size: 202 30 377
Fragments arranged by size:
377 202 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 207 346 609
Size: 207 139 263
Fragments arranged by size:
263 207 139
BsiEI CG_ry'CG
Cuts at: 0 107 131 164 609
Size: 107 24 33 445
Fragments arranged by size:
445 107 33 24
BsmI GAATG_Cn'
Cuts at: 0 278 609
Size: 278 331
BsmBI CGTCTCn'nnnn_
Cuts at: 0 219 609
Size: 219 390
Bsp1286I G_dGCh'C
Cuts at: 0 55 609
Size: 55 554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 429 609
Size: 429 180
BsrGI T'GTAC_A
Cuts at: 0 245 609
Size: 245 364
BstYI r'GATC_y
Cuts at: 0 315 609
Size: 315 294
BtgI C'CryG_G
Cuts at: 0 131 609
Size: 131 478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 90 609
Size: 90 519
EaeI y'GGCC_r
Cuts at: 0 104 128 134 161 609
Size: 104 24 6 27 448
Fragments arranged by size:
448 104 27 24 6
EagI C'GGCC_G
Cuts at: 0 104 128 161 609
Size: 104 24 33 448
Fragments arranged by size:
448 104 33 24
EarI CTCTTCn'nnn_
Cuts at: 0 274 473 609
Size: 274 199 136
Fragments arranged by size:
274 199 136
HaeII r_GCGC'y
Cuts at: 0 97 609
Size: 97 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 609
Size: 202 32 375
Fragments arranged by size:
375 202 32
KasI G'GCGC_C
Cuts at: 0 93 609
Size: 93 516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 609
Size: 330 279
MspA1I CmG'CkG
Cuts at: 0 45 109 133 157 609
Size: 45 64 24 24 452
Fragments arranged by size:
452 64 45 24 24
NarI GG'CG_CC
Cuts at: 0 94 609
Size: 94 515
NheI G'CTAG_C
Cuts at: 0 595 609
Size: 595 14
NotI GC'GGCC_GC
Cuts at: 0 128 161 609
Size: 128 33 448
Fragments arranged by size:
448 128 33
NsiI A_TGCA'T
Cuts at: 0 351 609
Size: 351 258
NspI r_CATG'y
Cuts at: 0 349 609
Size: 349 260
PstI C_TGCA'G
Cuts at: 0 393 609
Size: 393 216
SacII CC_GC'GG
Cuts at: 0 134 609
Size: 134 475
SfcI C'TryA_G
Cuts at: 0 389 609
Size: 389 220
SfoI GGC'GCC
Cuts at: 0 95 609
Size: 95 514
SspI AAT'ATT
Cuts at: 0 509 609
Size: 509 100
TatI w'GTAC_w
Cuts at: 0 245 325 609
Size: 245 80 284
Fragments arranged by size:
284 245 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 609
Size: 438 32 139
Fragments arranged by size:
438 139 32
XmnI GAAnn'nnTTC
Cuts at: 0 239 609
Size: 239 370
ZraI GAC'GTC
Cuts at: 0 213 609
Size: 213 396
Enzymes that do cut:
AatII AclI ApoI BanI BanII BbeI BbsI BclI
BfrBI BmtI BsaHI BsaXI BseRI BsiEI BsmI BsmBI
Bsp1286I BspMI BsrGI BstYI BtgI BtgZI EaeI EagI
EarI HaeII Hin4I KasI MmeI MspA1I NarI NheI
NotI NsiI NspI PstI SacII SfcI SfoI SspI
TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseYI
BsgI BsiHKAI BsiWI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
MslI NaeI NcoI NdeI NgoMIV NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI