Restriction map for
pFN21AB6860
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 339 GTAC GT^AC
AlwNI 256 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 176 AGCGCT AGC^GCT
Asp700I 270 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 320 TGGCCA TGG^CCA
BanI 225 GGCACC G^G[CT][GA]CC
BbvCI 388 GCTGAGG CCTCAGC(-5/-2)
BfaI 73 CTAG C^TAG
BglI 355 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmFI 164 GGGAC GGGAC(10/14)
BspMI 260 ACCTGC ACCTGC(4/8)
BsrBI 112 GAGCGG CCGCTC(-3/-3)
BstYI 485 AGATCC [GA]^GATC[CT]
DdeI 389 CTGAG C^T[ACGT]AG
DrdI 418 GACTTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 320 TGGCCA [CT]^GGCC[GA]
EarI 10 CTCTTC CTCTTC(1/4)
Eco47III 176 AGCGCT AGC^GCT
EcoT14I 323 CCATGG C^C[AT][AT]GG
Ksp632I 10 CTCTTC CTCTTC(1/4)
KspI 49 CCGCGG CCGC^GG
MaeI 73 CTAG C^TAG
MaeII 167 ACGT A^CGT
MaeIII 416 GTGAC ^GT[ACGT]AC
MflI 485 AGATCC [GA]^GATC[CT]
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
MslI 198 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 323 CCATGG C^CATGG
PflMI 323 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 257 AGGACCT [GA]G^G[AT]CC[CT]
PstI 220 CTGCAG CTGCA^G
PvuII 490 CAGCTG CAG^CTG
RsaI 339 GTAC GT^AC
SacI 303 GAGCTC GAGCT^C
SacII 49 CCGCGG CCGC^GG
SfcI 220 CTGCAG C^T[GA][CT]AG
SmlI 357 CTCAAG C^T[CT][GA]AG
StyI 323 CCATGG C^C[AT][AT]GG
Tsp45I 416 GTGAC ^GT[GC]AC
TspRI 507 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 323 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 455 CCAGCGACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 485 AGATCC [GA]^GATC[CT]
XmnI 270 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 171 GATCC GGATC(4/5)
486 GATCC
AspHI 247 GAGCAC G[AT]GC[AT]^C
303 GAGCTC
BsiHKAI 247 GAGCAC G[AT]GC[AT]^C
303 GAGCTC
DpnII 171 GATC ^GATC
486 GATC
HaeII 17 GGCGCT [GA]GCGC^[CT]
176 AGCGCT
HapII 58 CCGG C^CGG
108 CCGG
HgaI 236 GCGTC GACGC(5/10)
425 GCGTC
HinfI 382 GACTC G^A[ACGT]TC
460 GACTC
HpaII 58 CCGG C^CGG
108 CCGG
MboI 171 GATC ^GATC
486 GATC
MboII 213 GAAGA GAAGA(8/7)
11 TCTTC
MspI 58 CCGG C^CGG
108 CCGG
NdeII 171 GATC ^GATC
486 GATC
PleI 382 GACTC GAGTC(4/5)
460 GACTC
Sau3AI 171 GATC ^GATC
486 GATC
SfaNI 216 GATGC GCATC(5/9)
548 GCATC
SmaI 57 CCCGGG CCC^GGG
107 CCCGGG
XmaCI 57 CCCGGG C^CCGGG
107 CCCGGG
XmaI 57 CCCGGG C^CCGGG
107 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DraII DsaI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI MnlI
MspA1I MvaI MvnI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TseI