(Circular) (Six-base) MAPSORT of: pFN21AB8277.seq Check: 7500 from: 1 to: 5776 pFN21AB8277 5776 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2383 2383 Size: 5776 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2063 2496 3119 1937 Size: 126 433 623 4594 Fragments arranged by size: 4594 623 433 126 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 3839 5223 5491 1632 Size: 707 1500 1384 268 1917 Fragments arranged by size: 1917 1500 1384 707 268 AfeI AGC'GCT Cuts at: 3048 3048 Size: 5776 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2775 2955 4675 5652 1203 Size: 1572 180 1720 977 1327 Fragments arranged by size: 1720 1572 1327 977 180 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2670 2671 2702 2703 2670 Size: 1 31 1 5743 Fragments arranged by size: 5743 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2476 3519 5091 2476 Size: 1043 1572 3161 Fragments arranged by size: 3161 1572 1043 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2975 3085 3311 3463 5572 1493 Size: 1482 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 1482 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2643 3101 1870 Size: 73 700 458 4545 Fragments arranged by size: 4545 700 458 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2743 2776 2743 Size: 33 5743 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 3095 1391 Size: 1704 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2375 928 Size: 971 476 4329 Fragments arranged by size: 4329 971 476 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 2104 2922 1548 Size: 556 818 4402 Fragments arranged by size: 4402 818 556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4387 136 Size: 107 122 71 631 605 2715 1525 Fragments arranged by size: 2715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 2090 2163 3193 2090 Size: 73 1030 4673 Fragments arranged by size: 4673 1030 73 Bme1580I G_kGCm'C Cuts at: 1790 2727 3837 4993 1790 Size: 937 1110 1156 2573 Fragments arranged by size: 2573 1156 1110 937 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2021 1056 Size: 965 4811 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2623 2623 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2443 2543 2682 2717 4436 1239 Size: 1124 80 100 139 35 1719 2579 Fragments arranged by size: 2579 1719 1124 139 100 80 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2802 2941 4439 882 Size: 617 1303 139 1498 2219 Fragments arranged by size: 2219 1498 1303 617 139 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 2702 2927 5770 2702 Size: 225 2843 2708 Fragments arranged by size: 2843 2708 225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3060 3090 1178 Size: 30 165 30 1657 30 3864 Fragments arranged by size: 3864 1657 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5776 BseYI C'CCAG_C Cuts at: 2036 2419 4979 5475 2036 Size: 383 2560 496 2337 Fragments arranged by size: 2560 2337 496 383 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3837 3922 4993 3837 Size: 85 1071 4620 Fragments arranged by size: 4620 1071 85 BsmI GAATG_Cn' Cuts at: 2844 3287 3380 2844 Size: 443 93 5240 Fragments arranged by size: 5240 443 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2025 2339 2025 Size: 314 5462 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2727 3095 3837 3922 4993 1391 Size: 399 937 368 742 85 1071 2174 Fragments arranged by size: 2174 1071 937 742 399 368 85 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 2706 3665 5395 2706 Size: 959 1730 3087 Fragments arranged by size: 3087 1730 959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2383 3131 844 Size: 1539 748 3489 Fragments arranged by size: 3489 1539 748 BsrBI CCG'CTC Cuts at: 1394 1823 2167 2716 3663 1394 Size: 429 344 549 947 3507 Fragments arranged by size: 3507 947 549 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 2257 4271 4445 65 Size: 1183 1009 2014 174 1396 Fragments arranged by size: 2014 1396 1183 1009 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 2508 96 Size: 2412 3364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 2218 1184 Size: 1034 4742 BstXI CCAn_nnnn'nTGG Cuts at: 2209 2209 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2647 3106 3473 3858 3875 5327 5413 5425 Size: 675 459 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 675 459 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2950 3292 4086 4106 2950 Size: 342 794 20 4620 Fragments arranged by size: 4620 794 342 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2335 3223 4112 Size: 54 1425 50 275 18 505 888 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 888 505 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2587 2663 3706 1353 Size: 1234 76 1043 3423 Fragments arranged by size: 3423 1234 1043 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2363 2443 2543 2682 2717 3839 Size: 393 707 24 80 100 139 35 1122 Cuts at: 3839 4436 5223 5491 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1122 787 707 597 393 268 139 100 80 35 24 EcoNI CCTnn'n_nnAGG Cuts at: 2582 2582 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2917 2917 Size: 5776 EcoRI G'AATT_C Cuts at: 2975 3085 3463 2975 Size: 110 378 5288 Fragments arranged by size: 5288 378 110 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 2110 2344 2068 Size: 42 234 5500 Fragments arranged by size: 5500 234 42 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 3050 4923 1678 Size: 1372 1873 2531 Fragments arranged by size: 2531 1873 1372 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2362 2394 2420 2452 2901 Size: 32 135 32 790 32 26 32 449 Cuts at: 2901 2933 4417 4449 4491 4523 1373 Size: 32 1484 32 42 32 2626 Fragments arranged by size: 2626 1484 790 449 135 42 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 2230 3120 3366 669 Size: 988 281 292 890 246 3079 Fragments arranged by size: 3079 988 890 292 281 246 HindIII A'AGCT_T Cuts at: 748 2403 748 Size: 1655 4121 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2760 4890 5074 1305 Size: 1455 2130 184 2007 Fragments arranged by size: 2130 2007 1455 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2337 10 Size: 54 1425 343 505 3449 Fragments arranged by size: 3449 1425 505 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3734 4093 4252 518 Size: 648 2568 359 159 2042 Fragments arranged by size: 2568 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3192 3869 5017 5262 1606 Size: 1586 677 1148 245 2120 Fragments arranged by size: 2120 1586 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 NdeI CA'TA_TG Cuts at: 387 2128 387 Size: 1741 4035 NheI G'CTAG_C Cuts at: 1052 2017 1052 Size: 965 4811 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2779 2959 3134 4679 1132 Size: 97 1550 180 175 1545 2229 Fragments arranged by size: 2229 1550 1545 180 175 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 2775 2955 4675 2775 Size: 180 1720 3876 Fragments arranged by size: 3876 1720 180 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2917 2917 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 2481 3128 830 Size: 1651 647 3478 Fragments arranged by size: 3478 1651 647 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2587 2587 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SexAI A'CCwGG_T Cuts at: 2832 2832 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2477 3124 4259 4940 5131 826 Size: 219 1432 647 1135 681 191 1471 Fragments arranged by size: 1471 1432 1135 681 647 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 3215 513 Size: 552 939 1211 3074 Fragments arranged by size: 3074 1211 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2726 3803 3820 3973 4158 4603 325 Size: 1255 1146 1077 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1146 1077 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 3003 4022 Size: 274 80 33 51 1443 531 495 1019 Cuts at: 4022 5734 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1019 531 495 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2416 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI