Restriction map for
pFN21AB6840
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DpnII
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI MwoI NarI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 376 AAATTC [GA]^AATT[CT]
AflIII 239 ACGTGT A^C[GA][CT]GT
AgeI 505 ACCGGT A^CCGGT
AlwNI 544 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 457 GGGCCC GGGCC^C
ApoI 376 AAATTC [GA]^AATT[CT]
AspHI 306 GTGCTC G[AT]GC[AT]^C
BanI 210 GGTGCC G^G[CT][GA]CC
BbsI 394 GTCTTC GAAGAC(2/6)
BpuAI 394 GTCTTC GAAGAC(2/6)
BsaI 169 GAGACC GGTCTC(1/5)
BsaWI 505 ACCGGT [AT]^CCGG[AT]
BsiHKAI 306 GTGCTC G[AT]GC[AT]^C
BssSI 41 CACGAG CACGAG(-5/-1)
BstXI 223 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 250 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 447 GCGC GCG^C
FauI 30 GCGGG CCCGC(4/6)
HaeII 446 AGCGCC [GA]GCGC^[CT]
HgaI 434 GCGTC GACGC(5/10)
HhaI 447 GCGC GCG^C
HinP1I 447 GCGC G^CGC
HincII 235 GTCAAC GT[CT]^[GA]AC
HindII 235 GTCAAC GT[CT]^[GA]AC
HinfI 148 GAATC G^A[ACGT]TC
MspA1I 245 CAGCTG C[AC]G^C[GT]G
NaeI 250 GCCGGC GCC^GGC
NgoMIV 250 GCCGGC G^CCGGC
PinAI 505 ACCGGT A^CCGGT
PvuII 245 CAGCTG CAG^CTG
SapI 534 GAAGAGC GCTCTTC(1/4)
SmaI 347 CCCGGG CCC^GGG
TfiI 148 GAATC G^A[AT]TC
Tsp45I 103 GTCAC ^GT[GC]AC
TspRI 304 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 347 CCCGGG C^CCGGG
XmaI 347 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanII 44 GAGCCC G[GA]GC[CT]^C
457 GGGCCC
BcgI 43 CGAGCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
107 CGACTGTGCTGC
BcnI 347 CCCGG CC^[GC]GG
348 CCGGG
BsmAI 37 GAGAC GTCTC(1/5)
169 GAGAC
BsmFI 182 GGGAC GGGAC(10/14)
290 GGGAC
BspMI 254 GCAGGT ACCTGC(4/8)
390 GCAGGT
BsrFI 250 GCCGGC [GA]^CCGG[CT]
505 ACCGGT
BsrI 216 CCAGT ACTGG(1/-1)
543 CCAGT
Cfr10I 250 GCCGGC [GA]^CCGG[CT]
505 ACCGGT
DraII 457 GGGCCCC [GA]G^G[ACGT]CC[CT]
464 AGGCCCT
DsaI 65 CCATGG C^C[GA][CT]GG
527 CCATGG
EcoO109I 457 GGGCCCC [GA]G^G[ACGT]CC[CT]
464 AGGCCCT
EcoT14I 65 CCATGG C^C[AT][AT]GG
527 CCATGG
MaeII 239 ACGT A^CGT
356 ACGT
MaeIII 61 GTAAC ^GT[ACGT]AC
103 GTCAC
NciI 347 CCCGG CC^[GC]GG
348 CCGGG
NcoI 65 CCATGG C^CATGG
527 CCATGG
StyI 65 CCATGG C^C[AT][AT]GG
527 CCATGG
Tsp509I 334 AATT ^AATT
377 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI Cfr13I EarI EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI Ksp632I MboII MnlI MslI
MspI MvaI NlaIII NlaIV RsaI
Sau96I ScrFI TseI