(Circular) (Six-base) MAPSORT of: pFN21AB6840.seq Check: 1569 from: 1 to: 5278 pFN21AB6840 5278 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2250 2250 Size: 5278 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3088 278 Size: 53 83 186 936 1552 2468 Fragments arranged by size: 2468 1552 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2621 1937 Size: 684 4594 Acc65I G'GTAC_C Cuts at: 2599 2599 Size: 5278 AclI AA'CG_TT Cuts at: 1563 3405 3778 1563 Size: 1842 373 3063 Fragments arranged by size: 3063 1842 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 3341 4725 4993 1632 Size: 590 1119 1384 268 1917 Fragments arranged by size: 1917 1384 1119 590 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5081 AflIII A'CryG_T Cuts at: 1203 2244 4177 5154 1203 Size: 1041 1933 977 1327 Fragments arranged by size: 1933 1327 1041 977 AgeI A'CCGG_T Cuts at: 2510 4109 2510 Size: 1599 3679 AhdI GACnn_n'nnGTC Cuts at: 4007 4007 Size: 5278 AleI CACnn'nnGTG Cuts at: 1166 2114 1166 Size: 948 4330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2231 1184 Size: 1047 4231 AlwNI CAG_nnn'CTG Cuts at: 2554 3021 4593 2554 Size: 467 1572 3239 Fragments arranged by size: 3239 1572 467 ApaI G_GGCC'C Cuts at: 2466 2466 Size: 5278 ApaLI G'TGCA_C Cuts at: 3335 4491 3335 Size: 1156 4122 ApoI r'AATT_y Cuts at: 1493 2381 2587 2813 2965 5074 1493 Size: 888 206 226 152 2109 1697 Fragments arranged by size: 2109 1697 888 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 3832 160 Size: 872 2800 1606 Fragments arranged by size: 2800 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5278 AvaI C'yCGr_G Cuts at: 1870 1943 2292 2352 2421 2603 1870 Size: 73 349 60 69 182 4545 Fragments arranged by size: 4545 349 182 73 69 60 BamHI G'GATC_C Cuts at: 2608 2975 2608 Size: 367 4911 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2215 2599 4054 618 Size: 325 217 627 428 384 1455 1842 Fragments arranged by size: 1842 1455 627 428 384 325 217 BanII G_rGCy'C Cuts at: 1391 2053 2466 2597 1391 Size: 662 413 131 4072 Fragments arranged by size: 4072 662 413 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2392 928 Size: 971 493 3814 Fragments arranged by size: 3814 971 493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2037 2071 2101 2135 3469 3503 1903 Size: 34 100 34 30 34 1334 34 3678 Fragments arranged by size: 3678 1334 100 34 34 34 34 30 BciVI GTATCCnnnnn_n' Cuts at: 3170 4380 3170 Size: 1210 4068 BclI T'GATC_A Cuts at: 1548 1548 Size: 5278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3889 136 Size: 107 122 71 631 605 2217 1525 Fragments arranged by size: 2217 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5273 5273 Size: 5278 BlpI GC'TnA_GC Cuts at: 2695 2695 Size: 5278 Bme1580I G_kGCm'C Cuts at: 1790 2220 2466 3339 4495 1790 Size: 430 246 873 1156 2573 Fragments arranged by size: 2573 1156 873 430 246 BmgBI CAC'GTC Cuts at: 1318 2362 1318 Size: 1044 4234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2215 3009 3967 Size: 416 53 83 399 24 798 794 958 Cuts at: 3967 442 Size: 1753 Fragments arranged by size: 1753 958 798 794 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5278 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3938 1239 Size: 2699 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3403 4268 4566 3403 Size: 865 298 4115 Fragments arranged by size: 4115 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2168 3941 882 Size: 617 669 1773 2219 Fragments arranged by size: 2219 1773 669 617 BsaAI yAC'GTr Cuts at: 493 1204 3069 493 Size: 711 1865 2702 Fragments arranged by size: 2702 1865 711 BsaBI GATnn'nnATC Cuts at: 5272 5272 Size: 5278 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3085 3467 4102 275 Size: 53 83 186 936 1552 382 635 1451 Fragments arranged by size: 1552 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2510 3711 4109 4383 4530 4941 2510 Size: 1201 398 274 147 411 2847 Fragments arranged by size: 2847 1201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 1178 Size: 30 165 30 993 30 4030 Fragments arranged by size: 4030 993 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2465 2510 1186 Size: 1024 255 45 3954 Fragments arranged by size: 3954 1024 255 45 BseYI C'CCAG_C Cuts at: 4481 4977 4481 Size: 496 4782 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4705 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2728 3489 4517 1540 Size: 275 186 727 761 1028 2301 Fragments arranged by size: 2301 1028 761 727 275 186 BsiHKAI G_wGCw'C Cuts at: 2315 3339 3424 4495 2315 Size: 1024 85 1071 3098 Fragments arranged by size: 3098 1071 1024 85 BsmI GAATG_Cn' Cuts at: 2789 2882 2789 Size: 93 5185 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2220 2315 2466 2597 3339 3424 Size: 399 263 167 95 151 131 742 85 Cuts at: 3424 4495 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 263 167 151 131 95 85 BspEI T'CCGG_A Cuts at: 4941 4941 Size: 5278 BspHI T'CATG_A Cuts at: 3167 4897 3167 Size: 1730 3548 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2250 2386 2633 844 Size: 1406 136 247 3489 Fragments arranged by size: 3489 1406 247 136 BsrBI CCG'CTC Cuts at: 1394 1823 3165 1394 Size: 429 1342 3507 Fragments arranged by size: 3507 1342 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3773 3947 65 Size: 1183 2525 174 1396 Fragments arranged by size: 2525 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 2510 3922 4109 1700 Size: 110 445 255 1412 187 2869 Fragments arranged by size: 2869 1412 445 255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5278 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5278 BssSI C'ACGA_G Cuts at: 2046 3338 4350 2046 Size: 1292 1012 2974 Fragments arranged by size: 2974 1292 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5278 BstBI TT'CG_AA Cuts at: 4082 4082 Size: 5278 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5278 BstXI CCAn_nnnn'nTGG Cuts at: 2235 2235 Size: 5278 BstYI r'GATC_y Cuts at: 1972 2608 2975 3360 3377 4829 4915 4927 5273 Size: 636 367 385 17 1452 86 12 346 Cuts at: 5273 1972 Size: 1977 Fragments arranged by size: 1977 1452 636 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2532 513 Size: 552 939 66 462 3259 Fragments arranged by size: 3259 939 552 462 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4539 Fragments arranged by size: 4539 683 56 BtsI GCAGTG_nn' Cuts at: 2315 2794 3588 3608 2315 Size: 479 794 20 3985 Fragments arranged by size: 3985 794 479 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5278 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5243 DraI TTT'AAA Cuts at: 2582 2582 Size: 5278 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4285 809 Size: 507 2969 1802 Fragments arranged by size: 2969 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2725 3614 5243 Size: 54 1425 50 275 18 895 889 1629 Cuts at: 5243 8 Size: 43 Fragments arranged by size: 1629 1425 895 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2725 1537 Size: 275 913 4090 Fragments arranged by size: 4090 913 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2483 2492 2534 3208 1353 Size: 1130 9 42 674 3423 Fragments arranged by size: 3423 1130 674 42 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3865 4239 4385 387 Size: 1064 138 2276 374 146 1280 Fragments arranged by size: 2276 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 3341 3938 4725 4993 1239 Size: 393 590 1119 597 787 268 1524 Fragments arranged by size: 1524 1119 787 597 590 393 268 EcoO109I rG'GnC_Cy Cuts at: 2463 2470 2463 Size: 7 5271 EcoRI G'AATT_C Cuts at: 2587 2965 2587 Size: 378 4900 FspI TGC'GCA Cuts at: 3784 3784 Size: 5278 HaeII r_GCGC'y Cuts at: 1678 2455 4425 1678 Size: 777 1970 2531 Fragments arranged by size: 2531 1970 777 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3919 3951 3993 4025 1373 Size: 32 135 32 2347 32 42 32 2626 Fragments arranged by size: 2626 2347 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2242 2622 2868 669 Size: 988 281 304 380 246 3079 Fragments arranged by size: 3079 988 380 304 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5278 HpaI GTT'AAC Cuts at: 2868 2868 Size: 5278 KpnI G_GTAC'C Cuts at: 2603 2603 Size: 5278 MfeI C'AATT_G Cuts at: 2877 2877 Size: 5278 MluI A'CGCG_T Cuts at: 5154 5154 Size: 5278 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4392 4576 1305 Size: 3087 184 2007 Fragments arranged by size: 3087 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3456 Fragments arranged by size: 3456 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2114 2233 3236 3595 3754 518 Size: 648 909 39 119 1003 359 159 2042 Fragments arranged by size: 2042 1003 909 648 359 159 119 39 MspA1I CmG'CkG Cuts at: 1606 2252 2694 3371 4519 4764 1606 Size: 646 442 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 646 442 245 NaeI GCC'GGC Cuts at: 2257 2257 Size: 5278 NcoI C'CATG_G Cuts at: 513 1065 2004 2070 2532 513 Size: 552 939 66 462 3259 Fragments arranged by size: 3259 939 552 462 66 NdeI CA'TA_TG Cuts at: 387 387 Size: 5278 NgoMIV G'CCGG_C Cuts at: 2255 2255 Size: 5278 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5278 NotI GC'GGCC_GC Cuts at: 2725 2725 Size: 5278 NspI r_CATG'y Cuts at: 1132 1229 2636 4181 1132 Size: 97 1407 1545 2229 Fragments arranged by size: 2229 1545 1407 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5278 PciI A'CATG_T Cuts at: 4177 4177 Size: 5278 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5278 PmeI GTTT'AAAC Cuts at: 2582 2582 Size: 5278 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3347 3379 4860 4892 3347 Size: 32 1481 32 3733 Fragments arranged by size: 3733 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5278 PsiI TTA'TAA Cuts at: 2848 2848 Size: 5278 PspOMI G'GGCC_C Cuts at: 2462 2462 Size: 5278 PstI C_TGCA'G Cuts at: 830 2630 830 Size: 1800 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5278 PvuII CAG'CTG Cuts at: 2252 2252 Size: 5278 SalI G'TCGA_C Cuts at: 1936 2620 1936 Size: 684 4594 SapI GCTCTTCn'nnn_ Cuts at: 2534 2534 Size: 5278 SbfI CC_TGCA'GG Cuts at: 2630 2630 Size: 5278 ScaI AGT'ACT Cuts at: 3526 5238 3526 Size: 1712 3566 SfcI C'TryA_G Cuts at: 826 1045 2626 3761 4442 4633 826 Size: 219 1581 1135 681 191 1471 Fragments arranged by size: 1581 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5278 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5278 SmaI CCC'GGG Cuts at: 1872 2354 2605 1872 Size: 482 251 4545 Fragments arranged by size: 4545 482 251 SmlI C'TyrA_G Cuts at: 820 1017 1943 3382 4283 4545 820 Size: 197 926 1439 901 262 1553 Fragments arranged by size: 1553 1439 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3069 493 Size: 2576 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5278 SphI G_CATG'C Cuts at: 1132 2636 1132 Size: 1504 3774 SrfI GCCC'GGGC Cuts at: 2354 2354 Size: 5278 SspI AAT'ATT Cuts at: 5 52 3202 5 Size: 47 3150 2081 Fragments arranged by size: 3150 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5278 StyI C'CwwG_G Cuts at: 513 1065 2004 2070 2532 2717 513 Size: 552 939 66 462 185 3074 Fragments arranged by size: 3074 939 552 462 185 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 3305 3322 3475 3660 4105 325 Size: 1255 471 1254 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1254 471 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3524 5236 96 Size: 274 80 33 51 1443 1547 1712 138 Fragments arranged by size: 1712 1547 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2144 2176 5238 5270 2144 Size: 32 3062 32 2152 Fragments arranged by size: 3062 2152 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5278 XbaI T'CTAG_A Cuts at: 2614 2614 Size: 5278 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5278 XmaI C'CCGG_G Cuts at: 1870 2352 2603 1870 Size: 482 251 4545 Fragments arranged by size: 4545 482 251 XmnI GAAnn'nnTTC Cuts at: 3407 3407 Size: 5278 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3086 276 Size: 53 83 186 936 1552 2468 Fragments arranged by size: 2468 1552 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI XcmI