Restriction map for pFN21AB6790
 (Circular) (Six-base) MAPSORT of: pFN21AB6790.seq  Check: 4708  from: 1  to: 5170



pFN21AB6790  5170 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:43  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2980     278 
   Size:         53      83     186     936    1444    2468

  Fragments arranged by size: 

                2468    1444     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2513    1937 
   Size:        576    4594
 
Acc65I G'GTAC_C

Cuts at:   2491    2491 
   Size:       5170
 
AclI AA'CG_TT

Cuts at:   1563    3297    3670    1563 
   Size:       1734     373    3063

  Fragments arranged by size: 

                3063    1734     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2031    3233    4617    4885    1632 
   Size:        399    1202    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1202     399     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4973
 
AflIII A'CryG_T

Cuts at:   1203    4069    5046    1203 
   Size:       2866     977    1327

  Fragments arranged by size: 

                2866    1327     977
 
AgeI A'CCGG_T

Cuts at:   4001    4001 
   Size:       5170
 
AhdI GACnn_n'nnGTC

Cuts at:   3899    3899 
   Size:       5170
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5170
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2205    1184 
   Size:       1021    4149
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2285    2317    2285 
   Size:         32    5138
 
AlwNI CAG_nnn'CTG

Cuts at:   2913    4485    2913 
   Size:       1572    3598
 
ApaLI G'TGCA_C

Cuts at:   3227    4383    3227 
   Size:       1156    4014
 
ApoI r'AATT_y

Cuts at:   1493    2479    2705    2857    4966    1493 
   Size:        986     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     986     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3724     160 
   Size:        872    2692    1606

  Fragments arranged by size: 

                2692    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5170
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2495    1870 
   Size:         73     552    4545

  Fragments arranged by size: 

                4545     552      73
 
BamHI G'GATC_C

Cuts at:   2121    2500    2867    2121 
   Size:        379     367    4424

  Fragments arranged by size: 

                4424     379     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2491    3946     618 
   Size:        325     217     627     704    1455    1842

  Fragments arranged by size: 

                1842    1455     704     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2489    1391 
   Size:       1098    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4199
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3361    3395    1903 
   Size:         34    1424      34    3678

  Fragments arranged by size: 

                3678    1424      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3062    4272    3062 
   Size:       1210    3960
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5170
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3781     136 
   Size:        107     122      71     631     605    2109    1525

  Fragments arranged by size: 

                2109    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5165    5165 
   Size:       5170
 
BlpI GC'TnA_GC

Cuts at:   2587    2587 
   Size:       5170
 
Bme1580I G_kGCm'C

Cuts at:   1790    3231    4387    1790 
   Size:       1441    1156    2573

  Fragments arranged by size: 

                2573    1441    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5170
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2901    3859     442 
   Size:        416      53      83     399      24    1484     958    1753

  Fragments arranged by size: 

                1753    1484     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2046    2336    3830    1239 
   Size:        807     290    1494    2579

  Fragments arranged by size: 

                2579    1494     807     290
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2366    3295    4160    4458    2366 
   Size:        929     865     298    3078

  Fragments arranged by size: 

                3078     929     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3833     882 
   Size:        617    2334    2219

  Fragments arranged by size: 

                2334    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2961     493 
   Size:        711    1757    2702

  Fragments arranged by size: 

                2702    1757     711
 
BsaBI GATnn'nnATC

Cuts at:   5164    5164 
   Size:       5170
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2977    3359    3994     275 
   Size:         53      83     186     936    1444     382     635    1451

  Fragments arranged by size: 

                1451    1444     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2128    3603    4001    4275    4422    4833    2128 
   Size:       1475     398     274     147     411    2465

  Fragments arranged by size: 

                2465    1475     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4945

  Fragments arranged by size: 

                4945     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2078    2333    1186 
   Size:        892     255    4023

  Fragments arranged by size: 

                4023     892     255
 
BseYI C'CCAG_C

Cuts at:   4373    4869    4373 
   Size:        496    4674
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4597
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2620    3381    4409    1540 
   Size:        275     186     619     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     619     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3231    3316    4387    3231 
   Size:         85    1071    4014

  Fragments arranged by size: 

                4014    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2681    2774    2681 
   Size:         93    5077
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2489    3231    3316    4387    1391 
   Size:        399     699     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     699     399      85
 
BspEI T'CCGG_A

Cuts at:   2128    4833    2128 
   Size:       2705    2465
 
BspHI T'CATG_A

Cuts at:   3059    4789    3059 
   Size:       1730    3440
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2525     844 
   Size:       1681    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3057    1394 
   Size:        429    1234    3507

  Fragments arranged by size: 

                3507    1234     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3665    3839      65 
   Size:       1183    2417     174    1396

  Fragments arranged by size: 

                2417    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3814    4001    1700 
   Size:        110    2004     187    2869

  Fragments arranged by size: 

                2869    2004     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5170
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5170
 
BssSI C'ACGA_G

Cuts at:   3230    4242    3230 
   Size:       1012    4158
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5170
 
BstBI TT'CG_AA

Cuts at:   3974    3974 
   Size:       5170
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5170
 
BstYI r'GATC_y

Cuts at:   1972    2121    2500    2867    3252    3269    4721    4807    4819 
   Size:        149     379     367     385      17    1452      86      12

Cuts at:   4819    5165    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     379     367     346     149      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2117    2117 
   Size:       5170
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2247     513 
   Size:        552     939     243    3436

  Fragments arranged by size: 

                3436     939     552     243
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4431

  Fragments arranged by size: 

                4431     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2686    3480    3500    2686 
   Size:        794      20    4356

  Fragments arranged by size: 

                4356     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5170
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5135
 
DraI TTT'AAA

Cuts at:   2474    2474 
   Size:       5170
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4177     809 
   Size:        507    2861    1802

  Fragments arranged by size: 

                2861    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2233    2244    2425 
   Size:         54    1425      50     275      18     403      11     181

Cuts at:   2425    2617    3506    5135       8 
   Size:        192     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     403     275     192     181      54
                  50      43      18      11
 
EagI C'GGCC_G

Cuts at:   1537    1812    2617    1537 
   Size:        275     805    4090

  Fragments arranged by size: 

                4090     805     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2467    3100    1353 
   Size:       1114     633    3423

  Fragments arranged by size: 

                3423    1114     633
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3757    4131    4277     387 
   Size:       1064     138    2168     374     146    1280

  Fragments arranged by size: 

                2168    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2031    2046    2336    3233    3830    4617    4885 
   Size:        393     399      15     290     897     597     787     268

Cuts at:   4885    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     897     787     597     399     393     290     268
                  15
 
EcoRI G'AATT_C

Cuts at:   2479    2857    2479 
   Size:        378    4792
 
FspI TGC'GCA

Cuts at:   3676    3676 
   Size:       5170
 
HaeII r_GCGC'y

Cuts at:   1678    4317    1678 
   Size:       2639    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3811    3843    3885    3917    1373 
   Size:         32     135      32    2239      32      42      32    2626

  Fragments arranged by size: 

                2626    2239     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2514    2760     669 
   Size:        988     281     576     246    3079

  Fragments arranged by size: 

                3079     988     576     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5170
 
HpaI GTT'AAC

Cuts at:   2760    2760 
   Size:       5170
 
KpnI G_GTAC'C

Cuts at:   2495    2495 
   Size:       5170
 
MfeI C'AATT_G

Cuts at:   2769    2769 
   Size:       5170
 
MluI A'CGCG_T

Cuts at:   5046    5046 
   Size:       5170
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4284    4468    1305 
   Size:       2979     184    2007

  Fragments arranged by size: 

                2979    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2235    2246    2427      10 
   Size:         54    1425     343     403      11     181    2753

  Fragments arranged by size: 

                2753    1425     403     343     181      54      11
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3128    3487    3646     518 
   Size:        648    1962     359     159    2042

  Fragments arranged by size: 

                2042    1962     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2179    2586    3263    4411    4656    1606 
   Size:        573     407     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     573     407     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2247     513 
   Size:        552     939     243    3436

  Fragments arranged by size: 

                3436     939     552     243
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5170
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5170
 
NotI GC'GGCC_GC

Cuts at:   2617    2617 
   Size:       5170
 
NspI r_CATG'y

Cuts at:   1132    1229    2357    2528    4073    1132 
   Size:         97    1128     171    1545    2229

  Fragments arranged by size: 

                2229    1545    1128     171      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5170
 
PciI A'CATG_T

Cuts at:   4069    4069 
   Size:       5170
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2242    1108 
   Size:       1134    4036
 
PfoI T'CCnGG_A

Cuts at:   2304    2304 
   Size:       5170
 
PmeI GTTT'AAAC

Cuts at:   2474    2474 
   Size:       5170
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3239    3271    4752    4784    3239 
   Size:         32    1481      32    3625

  Fragments arranged by size: 

                3625    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5170
 
PsiI TTA'TAA

Cuts at:   2740    2740 
   Size:       5170
 
PstI C_TGCA'G

Cuts at:    830    2184    2522     830 
   Size:       1354     338    3478

  Fragments arranged by size: 

                3478    1354     338
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5170
 
PvuII CAG'CTG

Cuts at:   2179    2179 
   Size:       5170
 
SalI G'TCGA_C

Cuts at:   1936    2512    1936 
   Size:        576    4594
 
SbfI CC_TGCA'GG

Cuts at:   2522    2522 
   Size:       5170
 
ScaI AGT'ACT

Cuts at:   2240    3418    5130    2240 
   Size:       1178    1712    2280

  Fragments arranged by size: 

                2280    1712    1178
 
SfcI C'TryA_G

Cuts at:    826    1045    2095    2180    2518    3653    4334    4525     826 
   Size:        219    1050      85     338    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1050     681     338     219     191      85
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5170
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5170
 
SmaI CCC'GGG

Cuts at:   1872    2497    1872 
   Size:        625    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2381    3274    4175    4437     820 
   Size:        197     926     438     893     901     262    1553

  Fragments arranged by size: 

                1553     926     901     893     438     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2961     493 
   Size:       2468    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5170
 
SphI G_CATG'C

Cuts at:   1132    2357    2528    1132 
   Size:       1225     171    3774

  Fragments arranged by size: 

                3774    1225     171
 
SspI AAT'ATT

Cuts at:      5      52    3094       5 
   Size:         47    3042    2081

  Fragments arranged by size: 

                3042    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5170
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2247    2609     513 
   Size:        552     939     243     362    3074

  Fragments arranged by size: 

                3074     939     552     362     243
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2057    3197    3214    3367    3552    3997     325 
   Size:       1255     477    1140      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1140     477     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2238    3416    5128 
   Size:        274      80      33      51    1443     261    1178    1712

Cuts at:   5128      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1178     274     261     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5130    5162    5130 
   Size:         32    5138
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5170
 
XbaI T'CTAG_A

Cuts at:   2506    2506 
   Size:       5170
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5170
 
XmaI C'CCGG_G

Cuts at:   1870    2495    1870 
   Size:        625    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3299    3299 
   Size:       5170
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2978     276 
   Size:         53      83     186     936    1444    2468

  Fragments arranged by size: 

                2468    1444     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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