(Circular) (Six-base) MAPSORT of: pFN21AB6569.seq Check: 6873 from: 1 to: 5830 pFN21AB6569 5830 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3893 5277 5545 1632 Size: 2261 1384 268 1917 Fragments arranged by size: 2261 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 2050 2216 2558 2842 3573 5145 2050 Size: 166 342 284 731 1572 2735 Fragments arranged by size: 2735 1572 731 342 284 166 ApaI G_GGCC'C Cuts at: 2180 2262 2433 3019 2180 Size: 82 171 586 4991 Fragments arranged by size: 4991 586 171 82 ApaLI G'TGCA_C Cuts at: 2845 3887 5043 2845 Size: 1042 1156 3632 Fragments arranged by size: 3632 1156 1042 ApoI r'AATT_y Cuts at: 1493 3139 3365 3517 5626 1493 Size: 1646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1646 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2181 2605 3155 1870 Size: 73 238 424 550 4545 Fragments arranged by size: 4545 550 424 238 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2447 2591 3151 4606 618 Size: 325 217 627 660 144 560 1455 1842 Fragments arranged by size: 1842 1455 660 627 560 325 217 144 BanII G_rGCy'C Cuts at: 1391 2180 2262 2433 2468 2763 3019 3030 3149 Size: 789 82 171 35 295 256 11 119 Cuts at: 3149 1391 Size: 4072 Fragments arranged by size: 4072 789 295 256 171 119 82 35 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2081 928 Size: 971 182 4677 Fragments arranged by size: 4677 971 182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4021 4055 1903 Size: 34 50 34 2000 34 3678 Fragments arranged by size: 3678 2000 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2184 2490 4441 Size: 107 122 71 631 605 512 306 1951 Cuts at: 4441 136 Size: 1525 Fragments arranged by size: 1951 1525 631 605 512 306 122 107 71 BglII A'GATC_T Cuts at: 3063 5825 3063 Size: 2762 3068 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2180 2262 2433 2452 2849 3019 3891 5047 Size: 390 82 171 19 397 170 872 1156 Cuts at: 5047 1790 Size: 2573 Fragments arranged by size: 2573 1156 872 397 390 171 170 82 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2094 2246 2319 Size: 416 53 83 399 24 677 152 73 Cuts at: 2319 3022 3561 4519 442 Size: 703 539 958 1753 Fragments arranged by size: 1753 958 703 677 539 416 399 152 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 2583 2655 2711 4490 1239 Size: 1071 273 72 56 1779 2579 Fragments arranged by size: 2579 1779 1071 273 72 56 Bpu10I CC'TnA_GC Cuts at: 2942 2942 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 4820 5118 3955 Size: 865 298 4667 Fragments arranged by size: 4667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2709 2739 1178 Size: 30 165 30 1306 30 4269 Fragments arranged by size: 4269 1306 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2009 1186 Size: 823 5007 BseYI C'CCAG_C Cuts at: 2426 5033 5529 2426 Size: 2607 496 2727 Fragments arranged by size: 2727 2607 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2010 1163 Size: 573 274 4983 Fragments arranged by size: 4983 573 274 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2763 2849 3891 3976 5047 2763 Size: 86 1042 85 1071 3546 Fragments arranged by size: 3546 1071 1042 86 85 BsmI GAATG_Cn' Cuts at: 2628 2899 3341 3434 2628 Size: 271 442 93 5024 Fragments arranged by size: 5024 442 271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2262 2433 2452 2468 2763 2849 Size: 399 390 82 171 19 16 295 86 Cuts at: 2849 3019 3030 3149 3891 3976 5047 1391 Size: 170 11 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 295 171 170 119 86 85 82 19 16 11 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2683 3185 844 Size: 1839 502 3489 Fragments arranged by size: 3489 1839 502 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 2558 794 Size: 1764 4066 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstYI r'GATC_y Cuts at: 1972 3063 3160 3527 3912 3929 5381 5467 5479 Size: 1091 97 367 385 17 1452 86 12 Cuts at: 5479 5825 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1091 385 367 346 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2397 2496 2397 Size: 99 5731 BtgI C'CryG_G Cuts at: 513 1065 2568 513 Size: 552 1503 3775 Fragments arranged by size: 3775 1503 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2169 487 Size: 683 56 943 4148 Fragments arranged by size: 4148 943 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4140 4160 3346 Size: 794 20 5016 Fragments arranged by size: 5016 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2773 3277 4166 Size: 54 1425 50 275 18 943 504 889 Cuts at: 4166 5795 8 Size: 1629 43 Fragments arranged by size: 1629 1425 943 889 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2353 2768 3760 1353 Size: 665 335 415 992 3423 Fragments arranged by size: 3423 992 665 415 335 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2761 2761 Size: 5830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2583 2655 2711 3893 4490 5277 Size: 393 678 273 72 56 1182 597 787 Cuts at: 5277 5545 1239 Size: 268 1524 Fragments arranged by size: 1524 1182 787 678 597 393 273 268 72 56 EcoO109I rG'GnC_Cy Cuts at: 2176 2177 2197 2330 2366 2393 2492 2573 2600 Size: 1 20 133 36 27 99 81 27 Cuts at: 2600 3016 2176 Size: 416 4990 Fragments arranged by size: 4990 416 133 99 81 36 27 27 20 1 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 4977 1678 Size: 3299 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 3029 3061 4471 Size: 32 135 32 689 32 736 32 1410 Cuts at: 4471 4503 4545 4577 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1410 736 689 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2263 3033 4944 5128 1305 Size: 958 770 1911 184 2007 Fragments arranged by size: 2007 1911 958 770 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2775 10 Size: 54 1425 343 943 3065 Fragments arranged by size: 3065 1425 943 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3788 4147 4306 518 Size: 648 2622 359 159 2042 Fragments arranged by size: 2622 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2354 2839 2888 2911 3246 3923 5071 5316 Size: 748 485 49 23 335 677 1148 245 Cuts at: 5316 1606 Size: 2120 Fragments arranged by size: 2120 1148 748 677 485 335 245 49 23 NcoI C'CATG_G Cuts at: 513 1065 2568 513 Size: 552 1503 3775 Fragments arranged by size: 3775 1503 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NruI TCG'CGA Cuts at: 2154 2154 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2461 3188 4733 1132 Size: 97 1232 727 1545 2229 Fragments arranged by size: 2229 1545 1232 727 97 PasI CC'CwG_GG Cuts at: 1348 2050 2371 2578 3020 1348 Size: 702 321 207 442 4158 Fragments arranged by size: 4158 702 442 321 207 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PfoI T'CCnGG_A Cuts at: 2326 2632 2326 Size: 306 5524 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2957 2989 3899 3931 5412 5444 2957 Size: 32 910 32 1481 32 3343 Fragments arranged by size: 3343 1481 910 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2330 2366 2393 2573 2600 2197 Size: 133 36 27 180 27 5427 Fragments arranged by size: 5427 180 133 36 27 27 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspOMI G'GGCC_C Cuts at: 2176 2258 2429 3015 2176 Size: 82 171 586 4991 Fragments arranged by size: 4991 586 171 82 PstI C_TGCA'G Cuts at: 830 2221 3182 830 Size: 1391 961 3478 Fragments arranged by size: 3478 1391 961 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2354 2839 2911 2354 Size: 485 72 5273 Fragments arranged by size: 5273 485 72 SacI G_AGCT'C Cuts at: 2763 2763 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SanDI GG'GwC_CC Cuts at: 2197 2573 2600 2197 Size: 376 27 5427 Fragments arranged by size: 5427 376 27 SapI GCTCTTCn'nnn_ Cuts at: 2018 2768 2018 Size: 750 5080 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2217 3178 4313 4994 5185 826 Size: 219 1172 961 1135 681 191 1471 Fragments arranged by size: 1471 1172 1135 961 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2490 2490 Size: 5830 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 2607 3157 1872 Size: 735 550 4545 Fragments arranged by size: 4545 735 550 SmlI C'TyrA_G Cuts at: 820 1017 1943 3934 4835 5097 820 Size: 197 926 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 2461 3188 1132 Size: 1329 727 3774 Fragments arranged by size: 3774 1329 727 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 2484 2702 1517 Size: 967 218 4645 Fragments arranged by size: 4645 967 218 StyI C'CwwG_G Cuts at: 513 1065 2568 3269 513 Size: 552 1503 701 3074 Fragments arranged by size: 3074 1503 701 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 3874 4027 4212 4657 325 Size: 1255 2277 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2716 4076 5788 Size: 274 80 33 51 1443 739 1360 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1712 1443 1360 739 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 2605 3155 1870 Size: 735 550 4545 Fragments arranged by size: 4545 735 550 XmnI GAAnn'nnTTC Cuts at: 2795 3959 2795 Size: 1164 4666 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI