(Circular) (Six-base) MAPSORT of: pFN21AB6550.seq Check: 4230 from: 1 to: 5881 pFN21AB6550 5881 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2376 2460 3177 3944 5328 5596 1632 Size: 744 84 717 767 1384 268 1917 Fragments arranged by size: 1917 1384 767 744 717 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 2377 4780 5757 1203 Size: 1174 2403 977 1327 Fragments arranged by size: 2403 1327 1174 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 3624 5196 3624 Size: 1572 4309 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 2090 2527 3190 3416 3568 5677 1493 Size: 597 437 663 226 152 2109 1697 Fragments arranged by size: 2109 1697 663 597 437 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2892 3206 1870 Size: 73 115 834 314 4545 Fragments arranged by size: 4545 834 314 115 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 3202 4657 618 Size: 325 217 627 264 1151 1455 1842 Fragments arranged by size: 1842 1455 1151 627 325 264 217 BanII G_rGCy'C Cuts at: 1391 3155 3200 1391 Size: 1764 45 4072 Fragments arranged by size: 4072 1764 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3147 928 Size: 971 1248 3662 Fragments arranged by size: 3662 1248 971 BbvCI CC'TCA_GC Cuts at: 2777 2777 Size: 5881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2546 2580 4072 4106 1903 Size: 34 609 34 1492 34 3678 Fragments arranged by size: 3678 1492 609 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 2626 1548 Size: 1078 4803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4492 136 Size: 107 122 71 631 605 337 2483 1525 Fragments arranged by size: 2483 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 2054 3942 5098 1790 Size: 264 1888 1156 2573 Fragments arranged by size: 2573 1888 1156 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3612 4570 442 Size: 416 53 83 399 24 2195 958 1753 Fragments arranged by size: 2195 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2895 2895 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2908 4541 1239 Size: 1669 1633 2579 Fragments arranged by size: 2579 1669 1633 Bpu10I CC'TnA_GC Cuts at: 2777 2777 Size: 5881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2322 4006 4871 5169 2322 Size: 1684 865 298 3034 Fragments arranged by size: 3034 1684 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3021 4544 882 Size: 617 1522 1523 2219 Fragments arranged by size: 2219 1523 1522 617 BsaAI yAC'GTr Cuts at: 493 1204 2861 3672 493 Size: 711 1657 811 2702 Fragments arranged by size: 2702 1657 811 711 BsaBI GATnn'nnATC Cuts at: 2295 2887 5875 2295 Size: 592 2988 2301 Fragments arranged by size: 2988 2301 592 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5656 Fragments arranged by size: 5656 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2794 2887 3016 3163 1186 Size: 1608 93 129 147 3904 Fragments arranged by size: 3904 1608 147 129 93 BseYI C'CCAG_C Cuts at: 5084 5580 5084 Size: 496 5385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 1163 Size: 573 894 4414 Fragments arranged by size: 4414 894 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3155 3942 4027 5098 3155 Size: 787 85 1071 3938 Fragments arranged by size: 3938 1071 787 85 BsmI GAATG_Cn' Cuts at: 3392 3485 3392 Size: 93 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 3155 3200 3942 4027 5098 1391 Size: 399 264 1101 45 742 85 1071 2174 Fragments arranged by size: 2174 1101 1071 742 399 264 85 45 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 2140 2383 2602 3770 5500 2140 Size: 243 219 1168 1730 2521 Fragments arranged by size: 2521 1730 1168 243 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3236 844 Size: 2392 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2210 2922 3768 1394 Size: 429 387 712 846 3507 Fragments arranged by size: 3507 846 712 429 387 BsrDI GCAATG_nn' Cuts at: 65 1248 2283 4376 4550 65 Size: 1183 1035 2093 174 1396 Fragments arranged by size: 2093 1396 1183 1035 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4525 4712 1700 Size: 110 2715 187 2869 Fragments arranged by size: 2869 2715 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 2026 1925 Size: 101 5780 BssSI C'ACGA_G Cuts at: 3081 3941 4953 3081 Size: 860 1012 4009 Fragments arranged by size: 4009 1012 860 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5881 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2167 2813 3005 3211 3578 3963 3980 5432 Size: 195 646 192 206 367 385 17 1452 Cuts at: 5432 5518 5530 5876 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 646 385 367 346 206 195 192 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2262 513 Size: 552 1197 4132 Fragments arranged by size: 4132 1197 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2045 2816 2891 487 Size: 683 56 819 771 75 3477 Fragments arranged by size: 3477 819 771 683 75 56 BtsI GCAGTG_nn' Cuts at: 3397 4191 4211 3397 Size: 794 20 5067 Fragments arranged by size: 5067 794 20 ClaI AT'CG_AT Cuts at: 1554 2842 1554 Size: 1288 4593 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DraIII CAC_nnn'GTG Cuts at: 3083 3083 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 3328 4217 Size: 54 1425 50 275 18 483 1015 889 Cuts at: 4217 5846 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1015 889 483 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2737 2742 3153 3811 1353 Size: 984 400 5 411 658 3423 Fragments arranged by size: 3423 984 658 411 400 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4468 4842 4988 387 Size: 1064 138 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3153 3153 Size: 5881 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2376 2460 2908 3177 3944 4541 5328 Size: 393 744 84 448 269 767 597 787 Cuts at: 5328 5596 1239 Size: 268 1524 Fragments arranged by size: 1524 787 767 744 597 448 393 269 268 84 EcoO109I rG'GnC_Cy Cuts at: 2784 2916 2784 Size: 132 5749 EcoRI G'AATT_C Cuts at: 2527 3190 3568 2527 Size: 663 378 4840 Fragments arranged by size: 4840 663 378 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3026 3058 4522 4554 4596 Size: 32 135 32 1454 32 1464 32 42 Cuts at: 4596 4628 1373 Size: 32 2626 Fragments arranged by size: 2626 1464 1454 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2081 3225 3471 669 Size: 988 281 143 1144 246 3079 Fragments arranged by size: 3079 1144 988 281 246 143 HindIII A'AGCT_T Cuts at: 748 2675 748 Size: 1927 3954 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4995 5179 1305 Size: 3690 184 2007 Fragments arranged by size: 3690 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 10 Size: 54 1425 343 483 3576 Fragments arranged by size: 3576 1425 483 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2607 3839 4198 4357 518 Size: 648 1441 1232 359 159 2042 Fragments arranged by size: 2042 1441 1232 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3297 3974 5122 5367 1606 Size: 1691 677 1148 245 2120 Fragments arranged by size: 2120 1691 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2262 513 Size: 552 1197 4132 Fragments arranged by size: 4132 1197 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 3239 4784 1132 Size: 97 2010 1545 2229 Fragments arranged by size: 2229 2010 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2197 1108 Size: 939 150 4792 Fragments arranged by size: 4792 939 150 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PmlI CAC'GTG Cuts at: 2861 2861 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2784 2916 2784 Size: 132 5749 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PstI C_TGCA'G Cuts at: 830 2277 3233 830 Size: 1447 956 3478 Fragments arranged by size: 3478 1447 956 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 SacI G_AGCT'C Cuts at: 3155 3155 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SapI GCTCTTCn'nnn_ Cuts at: 3153 3153 Size: 5881 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2273 3229 4364 5045 5236 826 Size: 219 1228 956 1135 681 191 1471 Fragments arranged by size: 1471 1228 1135 956 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 2060 3208 1872 Size: 188 1148 4545 Fragments arranged by size: 4545 1148 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 2337 3985 4886 5148 820 Size: 197 926 394 1648 901 262 1553 Fragments arranged by size: 1648 1553 926 901 394 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2262 2418 3320 513 Size: 552 1197 156 902 3074 Fragments arranged by size: 3074 1197 902 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3908 3925 4078 4263 4708 325 Size: 1255 2328 17 153 185 445 1498 Fragments arranged by size: 2328 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4127 5839 96 Size: 274 80 33 51 1443 2150 1712 138 Fragments arranged by size: 2150 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5841 5873 5841 Size: 32 5849 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 2972 3217 2972 Size: 245 5636 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 2058 3206 1870 Size: 188 1148 4545 Fragments arranged by size: 4545 1148 188 XmnI GAAnn'nnTTC Cuts at: 2379 4010 2379 Size: 1631 4250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI