Restriction map for
pFN21AB6476
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BclI BcnI
BfrI BglI BglII BlnI BpmI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraII DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 724 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 190 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 334 CTCGGG C^[CT]CG[GA]G
BanI 44 GGTGCC G^G[CT][GA]CC
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BciVI 277 GGATAC GTATCC(6/5)
BlpI 220 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 220 GCTAAGC GC^T[ACGT]AGC
BsaAI 1036 TACGTG [CT]AC^GT[GA]
BsaI 898 GGTCTC GGTCTC(1/5)
BsmAI 899 GTCTC GTCTC(1/5)
BsmI 230 GAATGC GAATGC(1/-1)
BsoBI 334 CTCGGG C^[CT]CG[GA]G
BspMI 261 GCAGGT ACCTGC(4/8)
BsrDI 356 GCAATG GCAATG(2/0)
BstAPI 723 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 251 AGATCC [GA]^GATC[CT]
CelII 220 GCTAAGC GC^T[ACGT]AGC
DrdI 562 GACGAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 875 CCATGG C^C[GA][CT]GG
EarI 595 GAAGAG CTCTTC(1/4)
EcoT22I 359 ATGCAT ATGCA^T
FauI 282 CCCGC CCCGC(4/6)
Ksp632I 595 GAAGAG CTCTTC(1/4)
MaeII 1037 ACGT A^CGT
MflI 251 AGATCC [GA]^GATC[CT]
MslI 870 CATTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 758 CAGCTG C[AC]G^C[GT]G
NcoI 875 CCATGG C^CATGG
NlaIV 44 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 359 ATGCAT ATGCA^T
NspI 623 GCATGT [GA]CATG^[CT]
PvuII 758 CAGCTG CAG^CTG
StuI 796 AGGCCT AGG^CCT
TaqI 247 TCGA T^CGA
TthHB8I 247 TCGA T^CGA
XhoII 251 AGATCC [GA]^GATC[CT]
XmnI 190 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 252 GATCC GGATC(4/5)
445 GATCC
AseI 421 ATTAAT AT^TAAT
1030 ATTAAT
AsnI 421 ATTAAT AT^TAAT
1030 ATTAAT
AspHI 331 GAGCTC G[AT]GC[AT]^C
820 GAGCTC
AvaII 56 GGACC G^G[AT]CC
879 GGACC
BanII 331 GAGCTC G[GA]GC[CT]^C
820 GAGCTC
BmyI 331 GAGCTC G[AGT]GC[ACT]^C
820 GAGCTC
BsiHKAI 331 GAGCTC G[AT]GC[AT]^C
820 GAGCTC
Bsp1286I 331 GAGCTC G[AGT]GC[ACT]^C
820 GAGCTC
BssKI 53 CCTGG ^CC[ACGT]GG
519 CCTGG
BstF5I 764 CATCC GGATG(2/0)
3 GGATG
BstNI 53 CCTGG CC^[AT]GG
519 CCTGG
Cac8I 296 GCTTGC GC[ACGT]^[ACGT]GC
737 GCAAGC
Cfr13I 56 GGACC G^G[ACGT]CC
879 GGACC
DraI 474 TTTAAA TTT^AAA
940 TTTAAA
EcoRII 53 CCTGG ^CC[AT]GG
519 CCTGG
FokI 764 CATCC GGATG(9/13)
3 GGATG
HaeIII 511 GGCC GG^CC
797 GGCC
MaeIII 148 GTTAC ^GT[ACGT]AC
456 GTTAC
MvaI 53 CCTGG CC^[AT]GG
519 CCTGG
PshBI 421 ATTAAT AT^TAAT
1030 ATTAAT
PstI 721 CTGCAG CTGCA^G
730 CTGCAG
SacI 331 GAGCTC GAGCT^C
820 GAGCTC
Sau96I 56 GGACC G^G[ACGT]CC
879 GGACC
ScrFI 53 CCTGG CC^[ACGT]GG
519 CCTGG
XcmI 383 CCATGAAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1028 CCATTAATTACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AluI ApoI BbsI
BbvI BfaI BpuAI BsaJI BsiYI
BslI BsmFI BsrI DdeI DpnII
EcoT14I Fnu4HI HinfI HphI ItaI
MaeI MboI MboII MnlI MseI
MwoI NdeII NlaIII PleI Sau3AI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI