Restriction map for
pFN21AB6438
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbsI BbvCI
BcgI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SpeI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflIII 185 ACACGT A^C[GA][CT]GT
AvaI 311 CCCGGG C^[CT]CG[GA]G
BalI 150 TGGCCA TGG^CCA
BbeI 596 GGCGCC GGCGC^C
BciVI 917 GTATCC GTATCC(6/5)
BlpI 742 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 742 GCTGAGC GC^T[ACGT]AGC
BsgI 1000 CTGCAC GTGCAG(16/14)
BsoBI 311 CCCGGG C^[CT]CG[GA]G
BspHI 452 TCATGA T^CATGA
BsrBI 340 GAGCGG CCGCTC(-3/-3)
BsrDI 250 CATTGC GCAATG(2/0)
CelII 742 GCTGAGC GC^T[ACGT]AGC
EarI 448 CTCTTC CTCTTC(1/4)
EcoNI 615 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 596 GGCGCC [GA]GCGC^[CT]
HinfI 859 GACTC G^A[ACGT]TC
KasI 596 GGCGCC G^GCGCC
Ksp632I 448 CTCTTC CTCTTC(1/4)
MluNI 150 TGGCCA TGG^CCA
MscI 150 TGGCCA TGG^CCA
NarI 596 GGCGCC GG^CGCC
PleI 859 GACTC GAGTC(4/5)
PpuMI 314 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 452 TCATGA T^CATGA
SacI 16 GAGCTC GAGCT^C
ScaI 965 AGTACT AGT^ACT
SfoI 596 GGCGCC GGC^GCC
SmaI 311 CCCGGG CCC^GGG
SmlI 392 CTCAAG C^T[CT][GA]AG
SnaBI 914 TACGTA TAC^GTA
SphI 890 GCATGC GCATG^C
StuI 234 AGGCCT AGG^CCT
XcmI 125 CCACACGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 311 CCCGGG C^CCGGG
XmaI 311 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 954 GATCC GGATC(4/5)
987 GATCC
AlwNI 790 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
952 CAGATCCTG
AspHI 16 GAGCTC G[AT]GC[AT]^C
940 GAGCAC
AvaII 138 GGTCC G^G[AT]CC
315 GGACC
AviII 95 TGCGCA TGC^GCA
227 TGCGCA
BbrPI 186 CACGTG CAC^GTG
665 CACGTG
BglI 165 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
792 GCCCCTGCGGC
BpmI 531 CTCCAG CTGGAG(16/14)
76 CTGGAG
BsiHKAI 16 GAGCTC G[AT]GC[AT]^C
940 GAGCAC
BsmAI 376 GAGAC GTCTC(1/5)
520 GTCTC
BsrI 875 ACTGG ACTGG(1/-1)
821 CCAGT
BstAPI 110 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
789 GCAGCCCCTGC
BstYI 953 AGATCC [GA]^GATC[CT]
986 AGATCC
DraIII 111 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
186 CACGTGGTG
EcoT14I 620 CCAAGG C^C[AT][AT]GG
1014 CCTTGG
FspI 95 TGCGCA TGC^GCA
227 TGCGCA
HphI 870 GGTGA GGTGA(8/7)
260 TCACC
KspI 167 CCGCGG CCGC^GG
332 CCGCGG
MflI 953 AGATCC [GA]^GATC[CT]
986 AGATCC
MslI 348 CACGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
637 CACCTGCATG
NspI 642 GCATGT [GA]CATG^[CT]
890 GCATGC
PmaCI 186 CACGTG CAC^GTG
665 CACGTG
PmlI 186 CACGTG CAC^GTG
665 CACGTG
PvuII 133 CAGCTG CAG^CTG
499 CAGCTG
SacII 167 CCGCGG CCGC^GG
332 CCGCGG
StyI 620 CCAAGG C^C[AT][AT]GG
1014 CCTTGG
TspRI 241 CAGTG CA[GC]TG[ACGT][ACGT]^
822 CAGTG
XhoII 953 AGATCC [GA]^GATC[CT]
986 AGATCC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI BanI BanII BbvI BcnI
BmyI BsaAI BsaHI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BspMI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I