(Circular) (Six-base) MAPSORT of: pFN21AB6438.seq Check: 7127 from: 1 to: 5725 pFN21AB6438 5725 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2276 2652 2276 Size: 376 5349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 2873 2875 3788 5172 5440 1632 Size: 896 345 2 913 1384 268 1917 Fragments arranged by size: 1917 1384 913 896 345 268 2 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 2190 4624 5601 1203 Size: 987 2434 977 1327 Fragments arranged by size: 2434 1327 987 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4534 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2258 1184 Size: 1074 4651 AlwNI CAG_nnn'CTG Cuts at: 2800 2962 3468 5040 2800 Size: 162 506 1572 3485 Fragments arranged by size: 3485 1572 506 162 ApaI G_GGCC'C Cuts at: 2181 2316 2579 2826 2181 Size: 135 263 247 5080 Fragments arranged by size: 5080 263 247 135 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2316 3050 1870 Size: 73 373 734 4545 Fragments arranged by size: 4545 734 373 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2114 2601 3046 4501 Size: 325 217 627 257 70 487 445 1455 Cuts at: 4501 618 Size: 1842 Fragments arranged by size: 1842 1455 627 487 445 325 257 217 70 BanII G_rGCy'C Cuts at: 1391 2025 2181 2316 2579 2826 3044 1391 Size: 634 156 135 263 247 218 4072 Fragments arranged by size: 4072 634 263 247 218 156 135 BbeI G_GCGC'C Cuts at: 2605 2605 Size: 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2933 3617 4827 2933 Size: 684 1210 3831 Fragments arranged by size: 3831 1210 684 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2176 2803 4336 Size: 107 122 71 631 605 504 627 1533 Cuts at: 4336 136 Size: 1525 Fragments arranged by size: 1533 1525 631 627 605 504 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 2748 3142 2748 Size: 394 5331 Bme1580I G_kGCm'C Cuts at: 1790 2117 2181 2316 2535 2579 2591 2671 2826 Size: 327 64 135 219 44 12 80 155 Cuts at: 2826 3786 4942 1790 Size: 960 1156 2573 Fragments arranged by size: 2573 1156 960 327 219 155 135 80 64 44 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2820 2890 3456 Size: 416 53 83 399 24 1403 70 566 Cuts at: 3456 4414 442 Size: 958 1753 Fragments arranged by size: 1753 1403 958 566 416 399 83 70 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2521 4385 1239 Size: 863 419 1864 2579 Fragments arranged by size: 2579 1864 863 419 Bpu10I CC'TnA_GC Cuts at: 2019 2840 2019 Size: 821 4904 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2382 3850 4715 5013 2382 Size: 1468 865 298 3094 Fragments arranged by size: 3094 1468 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2193 2672 2921 3516 493 Size: 711 989 479 249 595 2702 Fragments arranged by size: 2702 989 711 595 479 249 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2226 2602 2886 3532 Size: 53 83 186 936 693 376 284 646 Cuts at: 3532 3914 4549 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 693 646 635 382 376 284 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5500 Fragments arranged by size: 5500 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2951 2954 2957 1186 Size: 1765 3 3 3954 Fragments arranged by size: 3954 1765 3 3 BseYI C'CCAG_C Cuts at: 2051 2060 2328 4928 5424 2051 Size: 9 268 2600 496 2352 Fragments arranged by size: 2600 2352 496 268 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2990 1163 Size: 573 1254 3898 Fragments arranged by size: 3898 1254 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2949 3786 3871 4942 2025 Size: 924 837 85 1071 2808 Fragments arranged by size: 2808 1071 924 837 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2117 2181 2316 2535 2579 2591 Size: 399 235 92 64 135 219 44 12 Cuts at: 2591 2671 2826 2949 3044 3786 3871 4942 1391 Size: 80 155 123 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 235 219 155 135 123 95 92 85 80 64 44 12 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 2457 3614 5344 2457 Size: 1157 1730 2838 Fragments arranged by size: 2838 1730 1157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2276 2628 2652 3080 844 Size: 1432 352 24 428 3489 Fragments arranged by size: 3489 1432 428 352 24 BsrBI CCG'CTC Cuts at: 1394 1823 2347 3612 1394 Size: 429 524 1265 3507 Fragments arranged by size: 3507 1265 524 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2254 4220 4394 65 Size: 1183 1006 1966 174 1396 Fragments arranged by size: 1966 1396 1183 1006 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 2056 2250 2286 2743 1925 Size: 131 194 36 457 4907 Fragments arranged by size: 4907 457 194 131 36 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 2121 2800 794 Size: 1327 679 3719 Fragments arranged by size: 3719 1327 679 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2958 2991 3055 3422 3807 3824 5276 5362 Size: 986 33 64 367 385 17 1452 86 Cuts at: 5362 5374 5720 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 986 385 367 346 86 64 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2152 2172 2337 513 Size: 552 1087 20 165 3901 Fragments arranged by size: 3901 1087 552 165 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 487 Size: 683 56 863 4123 Fragments arranged by size: 4123 863 683 56 BtsI GCAGTG_nn' Cuts at: 2252 3241 4035 4055 2252 Size: 989 794 20 3922 Fragments arranged by size: 3922 989 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2538 2573 529 Size: 35 1974 35 3681 Fragments arranged by size: 3681 1974 35 35 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DraIII CAC_nnn'GTG Cuts at: 2121 2196 2121 Size: 75 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2149 2155 2758 Size: 54 1425 50 275 18 319 6 603 Cuts at: 2758 3172 4061 5690 8 Size: 414 889 1629 43 Fragments arranged by size: 1629 1425 889 603 414 319 275 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 3655 1353 Size: 1106 1196 3423 Fragments arranged by size: 3423 1196 1106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2942 4312 4686 4832 387 Size: 1064 138 1353 1370 374 146 1280 Fragments arranged by size: 1370 1353 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2023 Size: 5725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2521 2528 2873 2875 3788 4385 Size: 393 470 419 7 345 2 913 597 Cuts at: 4385 5172 5440 1239 Size: 787 268 1524 Fragments arranged by size: 1524 913 787 597 470 419 393 345 268 7 2 EcoNI CCTnn'n_nnAGG Cuts at: 2624 2624 Size: 5725 EcoO109I rG'GnC_Cy Cuts at: 2177 2313 2320 2575 2686 2822 2836 3001 2177 Size: 136 7 255 111 136 14 165 4901 Fragments arranged by size: 4901 255 165 136 136 111 14 7 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 2102 2234 4231 2102 Size: 132 1997 3596 Fragments arranged by size: 3596 1997 132 HaeII r_GCGC'y Cuts at: 1678 2605 4872 1678 Size: 927 2267 2531 Fragments arranged by size: 2531 2267 927 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373 Size: 32 135 32 2794 32 42 32 2626 Fragments arranged by size: 2794 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KasI G'GCGC_C Cuts at: 2601 2601 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2763 4839 5023 1305 Size: 1458 2076 184 2007 Fragments arranged by size: 2076 2007 1458 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2157 10 Size: 54 1425 343 325 3578 Fragments arranged by size: 3578 1425 343 325 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2646 3683 4042 4201 518 Size: 648 1191 289 1037 359 159 2042 Fragments arranged by size: 2042 1191 1037 648 359 289 159 MspA1I CmG'CkG Cuts at: 1606 2051 2140 2174 2280 2339 2506 2932 3141 Size: 445 89 34 106 59 167 426 209 Cuts at: 3141 3818 4966 5211 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 445 426 245 209 167 106 89 59 34 NarI GG'CG_CC Cuts at: 2602 2602 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2651 2899 3083 4628 1132 Size: 97 1422 248 184 1545 2229 Fragments arranged by size: 2229 1545 1422 248 184 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PmlI CAC'GTG Cuts at: 2193 2672 2193 Size: 479 5246 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 2320 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PspOMI G'GGCC_C Cuts at: 2177 2312 2575 2822 2177 Size: 135 263 247 5080 Fragments arranged by size: 5080 263 247 135 PstI C_TGCA'G Cuts at: 830 2247 2931 2967 3077 830 Size: 1417 684 36 110 3478 Fragments arranged by size: 3478 1417 684 110 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2140 2506 2140 Size: 366 5359 SacI G_AGCT'C Cuts at: 2025 2025 Size: 5725 SacII CC_GC'GG Cuts at: 2175 2340 2175 Size: 165 5560 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 2972 3973 5685 2972 Size: 1001 1712 3012 Fragments arranged by size: 3012 1712 1001 SfcI C'TryA_G Cuts at: 826 1045 2243 2927 2963 3073 4208 4889 5080 Size: 219 1198 684 36 110 1135 681 191 Cuts at: 5080 826 Size: 1471 Fragments arranged by size: 1471 1198 1135 684 681 219 191 110 36 SfoI GGC'GCC Cuts at: 2603 2603 Size: 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 2318 3052 1872 Size: 446 734 4545 Fragments arranged by size: 4545 734 446 SmlI C'TyrA_G Cuts at: 820 1017 1943 2397 3829 4730 4992 820 Size: 197 926 454 1432 901 262 1553 Fragments arranged by size: 1553 1432 926 901 454 262 197 SnaBI TAC'GTA Cuts at: 493 2921 3516 493 Size: 2428 595 2702 Fragments arranged by size: 2702 2428 595 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 2899 3083 1132 Size: 1767 184 3774 Fragments arranged by size: 3774 1767 184 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 2241 1517 Size: 724 5001 StyI C'CwwG_G Cuts at: 513 1065 2625 3019 3164 513 Size: 552 1560 394 145 3074 Fragments arranged by size: 3074 1560 552 394 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3752 3769 3922 4107 4552 325 Size: 1255 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 2766 2970 Size: 274 80 33 51 1443 450 339 204 Cuts at: 2970 3971 5683 96 Size: 1001 1712 138 Fragments arranged by size: 1712 1443 1001 450 339 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5685 5717 5685 Size: 32 5693 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2137 2137 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 2316 3050 1870 Size: 446 734 4545 Fragments arranged by size: 4545 734 446 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI