Restriction map for
pFN21AB6430
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AhdI 391 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 322 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 687 CCTGAGG CC^T[ACGT]AGG
AspEI 391 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 54 CTCGGG C^[CT]CG[GA]G
BglI 535 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 509 AGATCT A^GATCT
BsgI 868 GTGCAG GTGCAG(16/14)
BsmFI 123 GTCCC GGGAC(10/14)
BsoBI 54 CTCGGG C^[CT]CG[GA]G
BssHII 627 GCGCGC G^CGCGC
BssSI 775 CTCGTG CACGAG(-5/-1)
BstYI 509 AGATCT [GA]^GATC[CT]
Bsu36I 687 CCTGAGG CC^T[ACGT]AGG
EagI 132 CGGCCG C^GGCCG
Eam1105I 391 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 132 CGGCCG C^GGCCG
Eco52I 132 CGGCCG C^GGCCG
Eco81I 687 CCTGAGG CC^T[ACGT]AGG
EcoNI 462 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 202 GACTC G^A[ACGT]TC
MflI 509 AGATCT [GA]^GATC[CT]
NcoI 158 CCATGG C^CATGG
PflMI 762 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 202 GACTC GAGTC(4/5)
PpuMI 470 AGGACCT [GA]G^G[AT]CC[CT]
SapI 1017 GAAGAGC GCTCTTC(1/4)
SbfI 790 CCTGCAGG CCTGCA^GG
SfaNI 618 GATGC GCATC(5/9)
Sse8387I 790 CCTGCAGG CCTGCA^GG
StuI 787 AGGCCT AGG^CCT
TaqI 194 TCGA T^CGA
TthHB8I 194 TCGA T^CGA
Van91I 762 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 509 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 648 ACACGT A^C[GA][CT]GT
857 ACACGT
ApaI 59 GGGCCC GGGCC^C
698 GGGCCC
AspHI 772 GAGCTC G[AT]GC[AT]^C
1020 GAGCTC
BbeI 27 GGCGCC GGCGC^C
231 GGCGCC
BbsI 3 GAAGAC GAAGAC(2/6)
915 GTCTTC
BcnI 125 CCCGG CC^[GC]GG
130 CCCGG
BpuAI 3 GAAGAC GAAGAC(2/6)
915 GTCTTC
BsaI 76 GAGACC GGTCTC(1/5)
781 GAGACC
BsiEI 132 CGGCCG CG[GA][CT]^CG
992 CGGTCG
BsiHKAI 772 GAGCTC G[AT]GC[AT]^C
1020 GAGCTC
BspMI 317 ACCTGC ACCTGC(4/8)
473 ACCTGC
BstAPI 180 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
321 GCAGACCCTGC
DpnII 510 GATC ^GATC
723 GATC
EaeI 132 CGGCCG [CT]^GGCC[GA]
533 CGGCCA
HgaI 6 GACGC GACGC(5/10)
407 GACGC
KasI 27 GGCGCC G^GCGCC
231 GGCGCC
MaeIII 652 GTGAC ^GT[ACGT]AC
979 GTCAC
MboI 510 GATC ^GATC
723 GATC
MslI 153 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
573 CACGTGCGTG
NarI 27 GGCGCC GG^CGCC
231 GGCGCC
NciI 125 CCCGG CC^[GC]GG
130 CCCGG
NdeII 510 GATC ^GATC
723 GATC
PvuII 821 CAGCTG CAG^CTG
971 CAGCTG
SacI 772 GAGCTC GAGCT^C
1020 GAGCTC
Sau3AI 510 GATC ^GATC
723 GATC
SfoI 27 GGCGCC GGC^GCC
231 GGCGCC
Tsp45I 652 GTGAC ^GT[GC]AC
979 GTCAC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BanII BbrPI BbvI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
Ksp632I KspI MaeII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV PmaCI PmlI PstI
SacII Sau96I ScrFI SfcI StyI
TseI TspRI XcmI