(Circular) (Six-base) MAPSORT of: pFN21AB6430.seq Check: 3193 from: 1 to: 5734 pFN21AB6430 5734 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3797 5181 5449 1632 Size: 2165 1384 268 1917 Fragments arranged by size: 2165 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 2653 2862 4633 5610 1203 Size: 1450 209 1771 977 1327 Fragments arranged by size: 1771 1450 1327 977 209 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 2401 4463 2401 Size: 2062 3672 AleI CACnn'nnGTG Cuts at: 1166 2582 1166 Size: 1416 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AlwNI CAG_nnn'CTG Cuts at: 2332 3477 5049 2332 Size: 1145 1572 3017 Fragments arranged by size: 3017 1572 1145 ApaI G_GGCC'C Cuts at: 2068 2707 2068 Size: 639 5095 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2059 3059 1870 Size: 73 116 1000 4545 Fragments arranged by size: 4545 1000 116 73 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 2236 2836 3055 4510 Size: 325 217 627 245 204 600 219 1455 Cuts at: 4510 618 Size: 1842 Fragments arranged by size: 1842 1455 627 600 325 245 219 217 204 BanII G_rGCy'C Cuts at: 1391 2068 2096 2151 2707 2781 2898 3029 3053 Size: 677 28 55 556 74 117 131 24 Cuts at: 3053 1391 Size: 4072 Fragments arranged by size: 4072 677 556 131 117 74 55 28 24 BbeI G_GCGC'C Cuts at: 2036 2240 2036 Size: 204 5530 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2913 928 Size: 971 116 898 3749 Fragments arranged by size: 3749 971 898 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 4345 136 Size: 107 122 71 631 605 874 1799 1525 Fragments arranged by size: 1799 1525 874 631 605 122 107 71 BglII A'GATC_T Cuts at: 2514 5729 2514 Size: 3215 2519 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2068 2579 2707 3795 4951 1790 Size: 278 511 128 1088 1156 2573 Fragments arranged by size: 2573 1156 1088 511 278 128 BmgBI CAC'GTC Cuts at: 1318 2773 1318 Size: 1455 4279 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2591 2627 2865 3009 4394 1239 Size: 1121 231 36 238 144 1385 2579 Fragments arranged by size: 2579 1385 1121 238 231 144 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2075 2780 4397 882 Size: 617 576 705 1617 2219 Fragments arranged by size: 2219 1617 705 617 576 BsaAI yAC'GTr Cuts at: 493 1204 2173 2580 2656 2865 3525 493 Size: 711 969 407 76 209 660 2702 Fragments arranged by size: 2702 969 711 660 407 209 76 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2033 2237 2413 Size: 53 83 186 936 479 21 204 176 Cuts at: 2413 3541 3923 4558 275 Size: 1128 382 635 1451 Fragments arranged by size: 1451 1128 936 635 479 382 204 186 176 83 53 21 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2403 2433 2468 2498 2564 Size: 30 165 30 1000 30 35 30 66 Cuts at: 2564 2594 2982 3012 1178 Size: 30 388 30 3900 Fragments arranged by size: 3900 1000 388 165 66 35 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2408 2864 1186 Size: 1105 117 456 4056 Fragments arranged by size: 4056 1105 456 117 BseYI C'CCAG_C Cuts at: 2700 4937 5433 2700 Size: 2237 496 3001 Fragments arranged by size: 3001 2237 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2894 1163 Size: 573 1158 4003 Fragments arranged by size: 4003 1158 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2140 3000 3184 3945 4973 1540 Size: 275 186 139 860 184 761 1028 2301 Fragments arranged by size: 2301 1028 860 761 275 186 184 139 BsiHKAI G_wGCw'C Cuts at: 2781 3029 3795 3880 4951 2781 Size: 248 766 85 1071 3564 Fragments arranged by size: 3564 1071 766 248 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2096 2151 2579 2707 2781 2898 Size: 399 278 28 55 428 128 74 117 Cuts at: 2898 3029 3053 3795 3880 4951 1391 Size: 131 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 428 399 278 131 128 117 85 74 55 28 24 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2331 2487 3089 844 Size: 1487 156 602 3489 Fragments arranged by size: 3489 1487 602 156 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 2632 1925 Size: 707 5027 BssSI C'ACGA_G Cuts at: 2780 3794 4806 2780 Size: 1014 1012 3708 Fragments arranged by size: 3708 1014 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2191 2332 794 Size: 1397 141 4196 Fragments arranged by size: 4196 1397 141 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstYI r'GATC_y Cuts at: 1972 2514 3064 3431 3816 3833 5285 5371 5383 Size: 542 550 367 385 17 1452 86 12 Cuts at: 5383 5729 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 550 542 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2693 2693 Size: 5734 BtgI C'CryG_G Cuts at: 513 1065 2140 2163 2231 2240 2422 513 Size: 552 1075 23 68 9 182 3825 Fragments arranged by size: 3825 1075 552 182 68 23 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2143 487 Size: 683 56 917 4078 Fragments arranged by size: 4078 917 683 56 BtsI GCAGTG_nn' Cuts at: 2502 3250 4044 4064 2502 Size: 748 794 20 4172 Fragments arranged by size: 4172 794 748 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2004 2039 529 Size: 35 1440 35 4224 Fragments arranged by size: 4224 1440 35 35 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2538 3181 Size: 54 1425 50 275 18 307 401 643 Cuts at: 3181 4070 5699 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 643 401 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2137 3181 1537 Size: 275 325 1044 4090 Fragments arranged by size: 4090 1044 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2268 3017 3664 1353 Size: 698 217 749 647 3423 Fragments arranged by size: 3423 749 698 647 217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2779 3027 2779 Size: 248 5486 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2591 2627 2865 3009 3797 4394 Size: 393 728 231 36 238 144 788 597 Cuts at: 4394 5181 5449 1239 Size: 787 268 1524 Fragments arranged by size: 1524 788 787 728 597 393 268 238 231 144 36 EcoNI CCTnn'n_nnAGG Cuts at: 2471 2471 Size: 5734 EcoO109I rG'GnC_Cy Cuts at: 2064 2065 2476 2704 2064 Size: 1 411 228 5094 Fragments arranged by size: 5094 411 228 1 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 2036 2121 2240 4881 1678 Size: 358 85 119 2641 2531 Fragments arranged by size: 2641 2531 358 119 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2387 2403 2419 Size: 32 135 32 620 32 163 16 16 Cuts at: 2419 2435 2468 2500 2982 3014 4375 4407 4449 Size: 16 33 32 482 32 1361 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 1361 620 482 163 135 42 33 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KasI G'GCGC_C Cuts at: 2032 2236 2032 Size: 204 5530 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 4848 5032 1305 Size: 1125 2418 184 2007 Fragments arranged by size: 2418 2007 1125 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3912 Fragments arranged by size: 3912 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2582 3692 4051 4210 518 Size: 648 996 420 1110 359 159 2042 Fragments arranged by size: 2042 1110 996 648 420 359 159 MspA1I CmG'CkG Cuts at: 1606 2142 2233 2424 2498 2746 2828 2978 3150 Size: 536 91 191 74 248 82 150 172 Cuts at: 3150 3827 4975 5220 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 536 248 245 191 172 150 91 82 74 NarI GG'CG_CC Cuts at: 2033 2237 2033 Size: 204 5530 NcoI C'CATG_G Cuts at: 513 1065 2163 513 Size: 552 1098 4084 Fragments arranged by size: 4084 1098 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 3092 4637 1132 Size: 97 1863 1545 2229 Fragments arranged by size: 2229 1863 1545 97 PasI CC'CwG_GG Cuts at: 1348 2707 1348 Size: 1359 4375 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 2773 1108 Size: 1665 4069 PfoI T'CCnGG_A Cuts at: 2486 2486 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PmlI CAC'GTG Cuts at: 2580 2656 2865 2580 Size: 76 209 5449 Fragments arranged by size: 5449 209 76 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 2476 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PspOMI G'GGCC_C Cuts at: 2064 2703 2064 Size: 639 5095 PstI C_TGCA'G Cuts at: 830 2328 2631 2800 3086 830 Size: 1498 303 169 286 3478 Fragments arranged by size: 3478 1498 303 286 169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2828 2978 2828 Size: 150 5584 SacI G_AGCT'C Cuts at: 2781 3029 2781 Size: 248 5486 SacII CC_GC'GG Cuts at: 2143 2234 2425 2143 Size: 91 191 5452 Fragments arranged by size: 5452 191 91 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SapI GCTCTTCn'nnn_ Cuts at: 3017 3017 Size: 5734 SbfI CC_TGCA'GG Cuts at: 2800 3086 2800 Size: 286 5448 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2324 2627 2796 2979 3082 4217 4898 Size: 219 1279 303 169 183 103 1135 681 Cuts at: 4898 5089 826 Size: 191 1471 Fragments arranged by size: 1471 1279 1135 681 303 219 191 183 169 103 SfoI GGC'GCC Cuts at: 2034 2238 2034 Size: 204 5530 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3838 4739 5001 820 Size: 197 926 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 2794 1517 Size: 1277 4457 StyI C'CwwG_G Cuts at: 513 1065 2015 2163 2292 3173 513 Size: 552 950 148 129 881 3074 Fragments arranged by size: 3074 950 881 552 148 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 2233 3761 3778 3931 4116 4561 Size: 1255 513 140 1528 17 153 185 445 Cuts at: 4561 325 Size: 1498 Fragments arranged by size: 1528 1498 1255 513 445 185 153 140 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2564 2596 5694 5726 2564 Size: 32 3098 32 2572 Fragments arranged by size: 3098 2572 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2170 2384 2941 2170 Size: 214 557 4963 Fragments arranged by size: 4963 557 214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI