(Circular) (Six-base) MAPSORT of: pFN21AB6422.seq Check: 9938 from: 1 to: 5722 pFN21AB6422 5722 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2441 2441 Size: 5722 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2667 3065 1937 Size: 730 398 4594 Fragments arranged by size: 4594 730 398 Acc65I G'GTAC_C Cuts at: 2495 3043 2495 Size: 548 5174 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2028 2988 2994 3785 5169 5437 1632 Size: 396 960 6 791 1384 268 1917 Fragments arranged by size: 1917 1384 960 791 396 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 4621 5598 1203 Size: 3418 977 1327 Fragments arranged by size: 3418 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AlwNI CAG_nnn'CTG Cuts at: 2528 2937 3465 5037 2528 Size: 409 528 1572 3213 Fragments arranged by size: 3213 1572 528 409 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 2223 2276 2387 3031 3257 3409 5518 1493 Size: 730 53 111 644 226 152 2109 1697 Fragments arranged by size: 2109 1697 730 644 226 152 111 53 AseI AT'TA_AT Cuts at: 160 1032 2991 4276 160 Size: 872 1959 1285 1606 Fragments arranged by size: 1959 1606 1285 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 2580 2701 3047 1870 Size: 73 637 121 346 4545 Fragments arranged by size: 4545 637 346 121 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2031 2064 2031 Size: 33 5689 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 3043 4498 618 Size: 325 217 627 708 548 1455 1842 Fragments arranged by size: 1842 1455 708 627 548 325 217 BanII G_rGCy'C Cuts at: 1391 3041 1391 Size: 1650 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2408 2954 928 Size: 971 509 546 3696 Fragments arranged by size: 3696 971 546 509 BbvCI CC'TCA_GC Cuts at: 2232 2232 Size: 5722 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 1548 Size: 5722 BfrBI ATG'CAT Cuts at: 2888 2888 Size: 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2512 4333 136 Size: 107 122 71 631 605 840 1821 1525 Fragments arranged by size: 1821 1525 840 631 605 122 107 71 BglII A'GATC_T Cuts at: 2409 5717 2409 Size: 3308 2414 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 3783 4939 1790 Size: 1993 1156 2573 Fragments arranged by size: 2573 1993 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2406 3453 4411 Size: 416 53 83 399 24 989 1047 958 Cuts at: 4411 442 Size: 1753 Fragments arranged by size: 1753 1047 989 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2426 1056 Size: 1370 4352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4382 1239 Size: 3143 2579 Bpu10I CC'TnA_GC Cuts at: 2232 2232 Size: 5722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2802 3847 4712 5010 2802 Size: 1045 865 298 3514 Fragments arranged by size: 3514 1045 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2546 4385 882 Size: 617 1047 1839 2219 Fragments arranged by size: 2219 1839 1047 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 5716 5716 Size: 5722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4155 4553 4827 4974 5385 4155 Size: 398 274 147 411 4492 Fragments arranged by size: 4492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5497 Fragments arranged by size: 5497 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5722 BseYI C'CCAG_C Cuts at: 2607 4925 5421 2607 Size: 2318 496 2908 Fragments arranged by size: 2908 2318 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3783 3868 4939 3783 Size: 85 1071 4566 Fragments arranged by size: 4566 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3041 3783 3868 4939 1391 Size: 399 1251 742 85 1071 2174 Fragments arranged by size: 2174 1251 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 3611 5341 3611 Size: 1730 3992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2441 3077 844 Size: 1597 636 3489 Fragments arranged by size: 3489 1597 636 BsrBI CCG'CTC Cuts at: 1394 1823 3609 1394 Size: 429 1786 3507 Fragments arranged by size: 3507 1786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2016 4217 4391 65 Size: 1183 768 2201 174 1396 Fragments arranged by size: 2201 1396 1183 768 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4366 4553 1700 Size: 110 2556 187 2869 Fragments arranged by size: 2869 2556 187 110 BsrGI T'GTAC_A Cuts at: 96 2478 96 Size: 2382 3340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2363 2409 3052 3419 3804 3821 5273 5359 Size: 391 46 643 367 385 17 1452 86 Cuts at: 5359 5371 5717 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 643 391 385 367 346 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4231 Fragments arranged by size: 4231 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2849 2863 487 Size: 683 56 1623 14 3346 Fragments arranged by size: 3346 1623 683 56 14 BtsI GCAGTG_nn' Cuts at: 2099 2265 2529 2557 2654 2796 3238 4032 4052 Size: 166 264 28 97 142 442 794 20 Cuts at: 4052 2099 Size: 3769 Fragments arranged by size: 3769 794 442 264 166 142 97 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2739 3169 4058 Size: 54 1425 50 275 18 909 430 889 Cuts at: 4058 5687 8 Size: 1629 43 Fragments arranged by size: 1629 1425 909 889 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2914 3652 1353 Size: 1561 738 3423 Fragments arranged by size: 3423 1561 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2988 2994 3785 4382 5169 5437 Size: 393 396 960 6 791 597 787 268 Cuts at: 5437 1239 Size: 1524 Fragments arranged by size: 1524 960 791 787 597 396 393 268 6 EcoNI CCTnn'n_nnAGG Cuts at: 2865 2865 Size: 5722 EcoRI G'AATT_C Cuts at: 2276 2387 3031 3409 2276 Size: 111 644 378 4589 Fragments arranged by size: 4589 644 378 111 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 2524 2556 2859 Size: 32 135 32 776 32 144 32 303 Cuts at: 2859 2891 4363 4395 4437 4469 1373 Size: 32 1472 32 42 32 2626 Fragments arranged by size: 2626 1472 776 303 144 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 3312 669 Size: 988 281 1128 246 3079 Fragments arranged by size: 3079 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 2059 748 Size: 1311 4411 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 2499 3047 2499 Size: 548 5174 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4836 5020 1305 Size: 3531 184 2007 Fragments arranged by size: 3531 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2741 10 Size: 54 1425 343 909 2991 Fragments arranged by size: 2991 1425 909 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2375 3680 4039 4198 518 Size: 648 843 366 1305 359 159 2042 Fragments arranged by size: 2042 1305 843 648 366 359 159 MspA1I CmG'CkG Cuts at: 1606 3138 3815 4963 5208 1606 Size: 1532 677 1148 245 2120 Fragments arranged by size: 2120 1532 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4231 Fragments arranged by size: 4231 939 552 NdeI CA'TA_TG Cuts at: 387 2575 387 Size: 2188 3534 NheI G'CTAG_C Cuts at: 1052 2422 1052 Size: 1370 4352 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NsiI A_TGCA'T Cuts at: 2890 2890 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 3080 4625 1132 Size: 97 1851 1545 2229 Fragments arranged by size: 2229 1851 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 1108 Size: 1041 4681 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 5722 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PspXI vC'TCGA_Gb Cuts at: 2580 2580 Size: 5722 PstI C_TGCA'G Cuts at: 830 3074 830 Size: 2244 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 3070 4205 4886 5077 826 Size: 219 2025 1135 681 191 1471 Fragments arranged by size: 2025 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2580 2817 3826 4727 4989 820 Size: 197 926 637 237 1009 901 262 1553 Fragments arranged by size: 1553 1009 926 901 637 262 237 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 2191 3646 5 Size: 47 2139 1455 2081 Fragments arranged by size: 2139 2081 1455 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5722 StyI C'CwwG_G Cuts at: 513 1065 2004 3161 513 Size: 552 939 1157 3074 Fragments arranged by size: 3074 1157 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3749 3766 3919 4104 4549 325 Size: 1255 2169 17 153 185 445 1498 Fragments arranged by size: 2169 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 3968 5680 Size: 274 80 33 51 1443 501 1490 1712 Cuts at: 5680 96 Size: 138 Fragments arranged by size: 1712 1490 1443 501 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5682 5714 5682 Size: 32 5690 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 2580 1943 Size: 637 5085 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 3851 3851 Size: 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI