(Circular) (Six-base) MAPSORT of: pFN21AB6518.seq Check: 3149 from: 1 to: 5863 pFN21AB6518 5863 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2299 2859 2962 2299 Size: 560 103 5200 Fragments arranged by size: 5200 560 103 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 3206 1937 Size: 517 752 4594 Fragments arranged by size: 4594 752 517 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2119 2201 2822 3926 5310 5578 1632 Size: 487 82 621 1104 1384 268 1917 Fragments arranged by size: 1917 1384 1104 621 487 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 2108 1166 Size: 942 4921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2888 1184 Size: 1704 4159 AlwNI CAG_nnn'CTG Cuts at: 2142 2380 2509 3606 5178 2142 Size: 238 129 1097 1572 2827 Fragments arranged by size: 2827 1572 1097 238 129 ApaI G_GGCC'C Cuts at: 2098 2098 Size: 5863 ApaLI G'TGCA_C Cuts at: 3920 5076 3920 Size: 1156 4707 ApoI r'AATT_y Cuts at: 1493 3172 3398 3550 5659 1493 Size: 1679 226 152 2109 1697 Fragments arranged by size: 2109 1697 1679 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2026 3188 1870 Size: 73 83 1162 4545 Fragments arranged by size: 4545 1162 83 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2944 3184 4639 618 Size: 325 217 627 1157 240 1455 1842 Fragments arranged by size: 1842 1455 1157 627 325 240 217 BanII G_rGCy'C Cuts at: 1391 2019 2098 2651 3182 1391 Size: 628 79 553 531 4072 Fragments arranged by size: 4072 628 553 531 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4892 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4054 4088 1903 Size: 34 2117 34 3678 Fragments arranged by size: 3678 2117 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3041 3056 4474 Size: 107 122 71 631 605 1369 15 1418 Cuts at: 4474 136 Size: 1525 Fragments arranged by size: 1525 1418 1369 631 605 122 107 71 15 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 2088 2098 2949 3924 5080 1790 Size: 298 10 851 975 1156 2573 Fragments arranged by size: 2573 1156 975 851 298 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 3082 3106 Size: 416 53 83 399 24 658 1007 24 Cuts at: 3106 3594 4552 442 Size: 488 958 1753 Fragments arranged by size: 1753 1007 958 658 488 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 2300 2807 4523 1239 Size: 805 256 507 1716 2579 Fragments arranged by size: 2579 1716 805 507 256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3988 4853 5151 3988 Size: 865 298 4700 Fragments arranged by size: 4700 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2122 2480 2522 4526 882 Size: 617 623 358 42 2004 2219 Fragments arranged by size: 2219 2004 623 617 358 42 BsaAI yAC'GTr Cuts at: 493 1204 2554 3654 493 Size: 711 1350 1100 2702 Fragments arranged by size: 2702 1350 1100 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2619 3670 4052 4687 Size: 53 83 186 936 1086 1051 382 635 Cuts at: 4687 275 Size: 1451 Fragments arranged by size: 1451 1086 1051 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2157 4296 4694 4968 5115 5526 2157 Size: 2139 398 274 147 411 2494 Fragments arranged by size: 2494 2139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5638 Fragments arranged by size: 5638 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2288 2422 2723 1186 Size: 931 171 134 301 4326 Fragments arranged by size: 4326 931 301 171 134 BseYI C'CCAG_C Cuts at: 2233 2560 3004 5066 5562 2233 Size: 327 444 2062 496 2534 Fragments arranged by size: 2534 2062 496 444 327 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 2693 2990 1163 Size: 573 543 414 297 4036 Fragments arranged by size: 4036 573 543 414 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2602 2763 3048 3313 4074 5102 Size: 275 186 601 161 285 265 761 1028 Cuts at: 5102 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 601 285 275 265 186 161 BsiHKAI G_wGCw'C Cuts at: 2651 3924 4009 5080 2651 Size: 1273 85 1071 3434 Fragments arranged by size: 3434 1273 1071 85 BsmI GAATG_Cn' Cuts at: 2388 3374 3467 2388 Size: 986 93 4784 Fragments arranged by size: 4784 986 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2088 2098 2651 2949 3182 3924 Size: 399 229 69 10 553 298 233 742 Cuts at: 3924 4009 5080 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 399 298 233 229 85 69 10 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2299 2466 2859 2962 3218 844 Size: 1455 167 393 103 256 3489 Fragments arranged by size: 3489 1455 393 256 167 103 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2406 2620 3105 4358 4532 65 Size: 1183 1158 214 485 1253 174 1396 Fragments arranged by size: 1396 1253 1183 1158 485 214 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3050 4507 4694 1700 Size: 110 1240 1457 187 2869 Fragments arranged by size: 2869 1457 1240 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 3124 794 Size: 2330 3533 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2113 Size: 5863 BstYI r'GATC_y Cuts at: 1972 2419 2830 3193 3560 3945 3962 5414 5500 Size: 447 411 363 367 385 17 1452 86 Cuts at: 5500 5512 5858 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 447 411 385 367 363 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2074 2652 2702 2757 3036 3095 513 Size: 552 1009 578 50 55 279 59 3281 Fragments arranged by size: 3281 1009 578 552 279 59 55 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2022 487 Size: 683 56 796 4328 Fragments arranged by size: 4328 796 683 56 BtsI GCAGTG_nn' Cuts at: 3379 4173 4193 3379 Size: 794 20 5049 Fragments arranged by size: 5049 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2371 2666 Size: 54 1425 50 275 18 270 271 295 Cuts at: 2666 2760 3033 3045 3087 3310 4199 5828 8 Size: 94 273 12 42 223 889 1629 43 Fragments arranged by size: 1629 1425 889 295 275 273 271 270 223 94 54 50 43 42 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2760 3045 3310 1537 Size: 275 948 285 265 4090 Fragments arranged by size: 4090 948 285 275 265 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 2183 3793 1353 Size: 803 27 1610 3423 Fragments arranged by size: 3423 1610 803 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2191 3092 4450 4824 4970 387 Size: 1064 138 602 901 1358 374 146 1280 Fragments arranged by size: 1358 1280 1064 901 602 374 146 138 EcoICRI GAG'CTC Cuts at: 2649 2649 Size: 5863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2119 2201 2300 2807 2822 3926 Size: 393 412 75 82 99 507 15 1104 Cuts at: 3926 4523 5310 5578 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1104 787 597 507 412 393 268 99 82 75 15 EcoNI CCTnn'n_nnAGG Cuts at: 2855 2855 Size: 5863 EcoO109I rG'GnC_Cy Cuts at: 2030 2095 2545 2638 2030 Size: 65 450 93 5255 Fragments arranged by size: 5255 450 93 65 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 5010 1678 Size: 3332 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2591 2623 4504 4536 4578 Size: 32 135 32 1019 32 1881 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 1881 1019 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 2720 2928 748 Size: 1972 208 3683 Fragments arranged by size: 3683 1972 208 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2150 4977 5161 1305 Size: 845 2827 184 2007 Fragments arranged by size: 2827 2007 845 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2102 2373 3089 10 Size: 54 1425 343 270 271 716 2784 Fragments arranged by size: 2784 1425 716 343 271 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2585 3821 4180 4339 518 Size: 648 942 477 1236 359 159 2042 Fragments arranged by size: 2042 1236 942 648 477 359 159 MspA1I CmG'CkG Cuts at: 1606 2139 2377 2704 3097 3279 3956 5104 5349 Size: 533 238 327 393 182 677 1148 245 Cuts at: 5349 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 533 393 327 245 238 182 NaeI GCC'GGC Cuts at: 3052 3052 Size: 5863 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5311 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NgoMIV G'CCGG_C Cuts at: 3050 3050 Size: 5863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3045 3310 3045 Size: 265 5598 NspI r_CATG'y Cuts at: 1132 1229 3085 3221 4766 1132 Size: 97 1856 136 1545 2229 Fragments arranged by size: 2229 1856 1545 136 97 PasI CC'CwG_GG Cuts at: 1348 2091 1348 Size: 743 5120 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 2380 1108 Size: 1272 4591 PfoI T'CCnGG_A Cuts at: 2010 2784 2010 Size: 774 5089 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2545 2638 2545 Size: 93 5770 PshAI GACnn'nnGTC Cuts at: 1613 2061 1613 Size: 448 5415 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PspOMI G'GGCC_C Cuts at: 2094 2094 Size: 5863 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2903 2935 2903 Size: 32 5831 PstI C_TGCA'G Cuts at: 830 2343 2694 2859 3215 830 Size: 1513 351 165 356 3478 Fragments arranged by size: 3478 1513 356 351 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2139 2377 2139 Size: 238 5625 RsrII CG'GwC_CG Cuts at: 3136 3136 Size: 5863 SacI G_AGCT'C Cuts at: 2651 2651 Size: 5863 SacII CC_GC'GG Cuts at: 2705 3098 2705 Size: 393 5470 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SanDI GG'GwC_CC Cuts at: 2638 2638 Size: 5863 SapI GCTCTTCn'nnn_ Cuts at: 2156 2183 2156 Size: 27 5836 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 2345 4111 5823 2345 Size: 1766 1712 2385 Fragments arranged by size: 2385 1766 1712 SfcI C'TryA_G Cuts at: 826 1045 2339 2690 2855 3211 4346 5027 5218 Size: 219 1294 351 165 356 1135 681 191 Cuts at: 5218 826 Size: 1471 Fragments arranged by size: 1471 1294 1135 681 356 351 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 3050 1700 Size: 1350 4513 SmaI CCC'GGG Cuts at: 1872 2028 3190 1872 Size: 156 1162 4545 Fragments arranged by size: 4545 1162 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 3967 4868 5130 820 Size: 197 926 2024 901 262 1553 Fragments arranged by size: 2024 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 2124 2678 1517 Size: 607 554 4702 Fragments arranged by size: 4702 607 554 StyI C'CwwG_G Cuts at: 513 1065 2415 2490 3302 513 Size: 552 1350 75 812 3074 Fragments arranged by size: 3074 1350 812 552 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3153 3890 3907 4060 4245 4690 325 Size: 1255 1573 737 17 153 185 445 1498 Fragments arranged by size: 1573 1498 1255 737 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 4109 5821 Size: 274 80 33 51 1443 366 1766 1712 Cuts at: 5821 96 Size: 138 Fragments arranged by size: 1766 1712 1443 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5823 5855 5823 Size: 32 5831 Tth111I GACn'n_nGTC Cuts at: 1316 2957 1316 Size: 1641 4222 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2088 2110 2676 3028 2088 Size: 22 566 352 4923 Fragments arranged by size: 4923 566 352 22 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 2026 3188 1870 Size: 156 1162 4545 Fragments arranged by size: 4545 1162 156 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI SexAI SfiI SfoI SrfI SwaI