Restriction map for
pFN21AB6518
(Linear) (Six-base) MAPSORT of: KIBB6518.seq Check: 8283 from: 1 to: 1158
KIBB6518.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 294 854 957 1158
Size: 294 560 103 201
Fragments arranged by size:
560 294 201 103
AccI GT'mk_AC
Cuts at: 0 449 1158
Size: 449 709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 196 817 1158
Size: 114 82 621 341
Fragments arranged by size:
621 341 114 82
AleI CACnn'nnGTG
Cuts at: 0 103 1158
Size: 103 1055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 883 1158
Size: 883 275
AlwNI CAG_nnn'CTG
Cuts at: 0 137 375 504 1158
Size: 137 238 129 654
Fragments arranged by size:
654 238 137 129
ApaI G_GGCC'C
Cuts at: 0 93 1158
Size: 93 1065
AvaI C'yCGr_G
Cuts at: 0 21 1158
Size: 21 1137
BanI G'GyrC_C
Cuts at: 0 939 1158
Size: 939 219
BanII G_rGCy'C
Cuts at: 0 14 93 646 1158
Size: 14 79 553 512
Fragments arranged by size:
553 512 79 14
BglI GCCn_nnn'nGGC
Cuts at: 0 1036 1051 1158
Size: 1036 15 107
Fragments arranged by size:
1036 107 15
Bme1580I G_kGCm'C
Cuts at: 0 83 93 944 1158
Size: 83 10 851 214
Fragments arranged by size:
851 214 83 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 1077 1101 1158
Size: 70 1007 24 57
Fragments arranged by size:
1007 70 57 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 295 802 1158
Size: 39 256 507 356
Fragments arranged by size:
507 356 256 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 117 475 517 1158
Size: 117 358 42 641
Fragments arranged by size:
641 358 117 42
BsaAI yAC'GTr
Cuts at: 0 549 1158
Size: 549 609
BsaHI Gr'CG_yC
Cuts at: 0 614 1158
Size: 614 544
BsaWI w'CCGG_w
Cuts at: 0 152 1158
Size: 152 1006
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 283 417 718 1158
Size: 112 171 134 301 440
Fragments arranged by size:
440 301 171 134 112
BseYI C'CCAG_C
Cuts at: 0 228 555 999 1158
Size: 228 327 444 159
Fragments arranged by size:
444 327 228 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 688 985 1158
Size: 274 414 297 173
Fragments arranged by size:
414 297 274 173
BsiEI CG_ry'CG
Cuts at: 0 597 758 1043 1158
Size: 597 161 285 115
Fragments arranged by size:
597 285 161 115
BsiHKAI G_wGCw'C
Cuts at: 0 646 1158
Size: 646 512
BsmI GAATG_Cn'
Cuts at: 0 383 1158
Size: 383 775
Bsp1286I G_dGCh'C
Cuts at: 0 14 83 93 646 944 1158
Size: 14 69 10 553 298 214
Fragments arranged by size:
553 298 214 69 14 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 461 854 957 1158
Size: 294 167 393 103 201
Fragments arranged by size:
393 294 201 167 103
BsrDI GCAATG_nn'
Cuts at: 0 401 615 1100 1158
Size: 401 214 485 58
Fragments arranged by size:
485 401 214 58
BsrFI r'CCGG_y
Cuts at: 0 1045 1158
Size: 1045 113
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1119 1158
Size: 1119 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 1158
Size: 108 1050
BstYI r'GATC_y
Cuts at: 0 414 825 1158
Size: 414 411 333
Fragments arranged by size:
414 411 333
BtgI C'CryG_G
Cuts at: 0 69 647 697 752 1031 1090 1158
Size: 69 578 50 55 279 59 68
Fragments arranged by size:
578 279 69 68 59 55 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 17 1158
Size: 17 1141
EaeI y'GGCC_r
Cuts at: 0 95 366 661 755 1028 1040 1082 1158
Size: 95 271 295 94 273 12 42 76
Fragments arranged by size:
295 273 271 95 94 76 42 12
EagI C'GGCC_G
Cuts at: 0 755 1040 1158
Size: 755 285 118
Fragments arranged by size:
755 285 118
EarI CTCTTCn'nnn_
Cuts at: 0 151 178 1158
Size: 151 27 980
Fragments arranged by size:
980 151 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 186 1087 1158
Size: 186 901 71
Fragments arranged by size:
901 186 71
EcoICRI GAG'CTC
Cuts at: 0 644 1158
Size: 644 514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 114 196 295 802 817 1158
Size: 39 75 82 99 507 15 341
Fragments arranged by size:
507 341 99 82 75 39 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 850 1158
Size: 850 308
EcoO109I rG'GnC_Cy
Cuts at: 0 25 90 540 633 1158
Size: 25 65 450 93 525
Fragments arranged by size:
525 450 93 65 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 586 618 1158
Size: 586 32 540
Fragments arranged by size:
586 540 32
HindIII A'AGCT_T
Cuts at: 0 715 923 1158
Size: 715 208 235
Fragments arranged by size:
715 235 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 1158
Size: 145 1013
MscI TGG'CCA
Cuts at: 0 97 368 1084 1158
Size: 97 271 716 74
Fragments arranged by size:
716 271 97 74
MslI CAynn'nnrTG
Cuts at: 0 103 580 1158
Size: 103 477 578
Fragments arranged by size:
578 477 103
MspA1I CmG'CkG
Cuts at: 0 134 372 699 1092 1158
Size: 134 238 327 393 66
Fragments arranged by size:
393 327 238 134 66
NaeI GCC'GGC
Cuts at: 0 1047 1158
Size: 1047 111
NgoMIV G'CCGG_C
Cuts at: 0 1045 1158
Size: 1045 113
NotI GC'GGCC_GC
Cuts at: 0 1040 1158
Size: 1040 118
NspI r_CATG'y
Cuts at: 0 1080 1158
Size: 1080 78
PasI CC'CwG_GG
Cuts at: 0 86 1158
Size: 86 1072
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 1158
Size: 375 783
PfoI T'CCnGG_A
Cuts at: 0 5 779 1158
Size: 5 774 379
Fragments arranged by size:
774 379 5
PpuMI rG'GwC_Cy
Cuts at: 0 540 633 1158
Size: 540 93 525
Fragments arranged by size:
540 525 93
PshAI GACnn'nnGTC
Cuts at: 0 56 1158
Size: 56 1102
PspOMI G'GGCC_C
Cuts at: 0 89 1158
Size: 89 1069
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 898 930 1158
Size: 898 32 228
Fragments arranged by size:
898 228 32
PstI C_TGCA'G
Cuts at: 0 338 689 854 1158
Size: 338 351 165 304
Fragments arranged by size:
351 338 304 165
PvuII CAG'CTG
Cuts at: 0 134 372 1158
Size: 134 238 786
Fragments arranged by size:
786 238 134
RsrII CG'GwC_CG
Cuts at: 0 1131 1158
Size: 1131 27
SacI G_AGCT'C
Cuts at: 0 646 1158
Size: 646 512
SacII CC_GC'GG
Cuts at: 0 700 1093 1158
Size: 700 393 65
Fragments arranged by size:
700 393 65
SanDI GG'GwC_CC
Cuts at: 0 633 1158
Size: 633 525
SapI GCTCTTCn'nnn_
Cuts at: 0 151 178 1158
Size: 151 27 980
Fragments arranged by size:
980 151 27
ScaI AGT'ACT
Cuts at: 0 340 1158
Size: 340 818
SfcI C'TryA_G
Cuts at: 0 334 685 850 1158
Size: 334 351 165 308
Fragments arranged by size:
351 334 308 165
SgrAI Cr'CCGG_yG
Cuts at: 0 1045 1158
Size: 1045 113
SmaI CCC'GGG
Cuts at: 0 23 1158
Size: 23 1135
StuI AGG'CCT
Cuts at: 0 119 673 1158
Size: 119 554 485
Fragments arranged by size:
554 485 119
StyI C'CwwG_G
Cuts at: 0 410 485 1158
Size: 410 75 673
Fragments arranged by size:
673 410 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1148 1158
Size: 1148 10
TatI w'GTAC_w
Cuts at: 0 338 1158
Size: 338 820
Tth111I GACn'n_nGTC
Cuts at: 0 952 1158
Size: 952 206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 83 105 671 1023 1158
Size: 83 22 566 352 135
Fragments arranged by size:
566 352 135 83 22
XmaI C'CCGG_G
Cuts at: 0 21 1158
Size: 21 1137
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI AvaI
BanI BanII BglI Bme1580I BmrI BpmI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI
BtgZI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I HindIII MmeI MscI MslI MspA1I NaeI
NgoMIV NotI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PsrI PstI PvuII RsrII SacI SacII SanDI
SapI ScaI SfcI SgrAI SmaI StuI StyI TaqII
TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI
BsaXI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PvuI SalI SbfI
SexAI SfiI SfoI SgfI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI XhoI XmnI ZraI