Restriction map for pFN21AB6475
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbrPI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1407I    BspEI       
BspHI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
CelII       CpoI        DraI        DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoT14I     
EcoT22I     FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MluI        MluNI       MroI        MscI        
NaeI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   59 enzymes) #
###################################

AcyI           64  GGCGCC               G[GA]^CG[CT]C
AflIII        297  ACATGT               A^C[GA][CT]GT
Alw44I        246  GTGCAC               G^TGCAC
AlwI          600  GGATC                GGATC(4/5)
ApaLI         246  GTGCAC               G^TGCAC
AvaII         903  GGACC                G^G[AT]CC
BbeI           64  GGCGCC               GGCGC^C
BbsI          721  GAAGAC               GAAGAC(2/6)
BbvCI         192  GCTGAGG              CCTCAGC(-5/-2)
BclI          675  TGATCA               T^GATCA
BpuAI         721  GAAGAC               GAAGAC(2/6)
BsaBI         670  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          64  GGCGCC               G[GA]^CG[CT]C
BsaI          205  GAGACC               GGTCTC(1/5)
BseRI         151  GAGGAG               GAGGAG(10/8)
BsgI          139  GTGCAG               GTGCAG(16/14)
BsmI           40  GAATGC               GAATGC(1/-1)
BspDI         805  ATCGAT               AT^CGAT
BspLU11I      297  ACATGT               A^CATGT
BspMI         938  ACCTGC               ACCTGC(4/8)
BsrBI         163  CCGCTC               CCGCTC(-3/-3)
BsrFI         145  ACCGGC               [GA]^CCGG[CT]
BstEII        456  GGTGACC              G^GT[ACGT]ACC
BstF5I        174  CATCC                GGATG(2/0)
BstPI         456  GGTGACC              G^GT[ACGT]ACC
BstYI         600  GGATCT               [GA]^GATC[CT]
Cfr10I        145  ACCGGC               [GA]^CCGG[CT]
ClaI          805  ATCGAT               AT^CGAT
DraII         902  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        543  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          596  CCGTGG               C^C[GA][CT]GG
EcoO109I      902  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       456  GGTGACC              G^GT[ACGT]ACC
EcoRV         802  GATATC               GAT^ATC
FbaI          675  TGATCA               T^GATCA
FokI          174  CATCC                GGATG(9/13)
HaeII          64  GGCGCC               [GA]GCGC^[CT]
HapII         146  CCGG                 C^CGG
Hin1I          64  GGCGCC               G[GA]^CG[CT]C
HpaII         146  CCGG                 C^CGG
KasI           64  GGCGCC               G^GCGCC
MamI          670  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          782  CAATTG               C^AATTG
MflI          600  GGATCT               [GA]^GATC[CT]
MseI          848  TTAA                 T^TAA
MspA1I        465  CAGCGG               C[AC]G^C[GT]G
MspI          146  CCGG                 C^CGG
MunI          782  CAATTG               C^AATTG
NarI           64  GGCGCC               GG^CGCC
NruI          429  TCGCGA               TCG^CGA
PleI          707  GACTC                GAGTC(4/5)
PpuMI         902  AGGACCT              [GA]G^G[AT]CC[CT]
SfoI           64  GGCGCC               GGC^GCC
SphI          158  GCATGC               GCATG^C
TfiI          931  GATTC                G^A[AT]TC
Tru9I         848  TTAA                 T^TAA
Tsp509I       783  AATT                 ^AATT
XcmI          173  CCATCCGCGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         600  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI           36  GTAC                 GT^AC
              552  GTAC                
AlwNI         338  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              901  CAGGACCTG           
Asp700I        75  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              631  GAAATGTTTC          
BanI           64  GGCGCC               G^G[CT][GA]CC
              197  GGCACC              
BbvI          464  GCAGC                GCAGC(8/12)
              617  GCTGC               
BsmAI         205  GAGAC                GTCTC(1/5)
              885  GTCTC               
BsrI          725  ACTGG                ACTGG(1/-1)
              576  CCAGT               
BstXI         317  CCACTTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              590  CCACCCCCGTGG        
Cfr13I         87  GGCCC                G^G[ACGT]CC
              903  GGACC               
DdeI          193  CTGAG                C^T[ACGT]AG
              531  CTCAG               
HinfI         707  GACTC                G^A[ACGT]TC
              931  GATTC               
HphI          456  GGTGA                GGTGA(8/7)
              888  TCACC               
MaeII         224  ACGT                 A^CGT
              698  ACGT                
MslI          450  CATTCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              591  CACCCCCGTG          
NlaIII        159  CATG                 CATG^
              298  CATG                
NlaIV          64  GGCGCC               GG[ACGT]^[ACGT]CC
              197  GGCACC              
NspI          158  GCATGC               [GA]CATG^[CT]
              297  ACATGT              
PflMI         337  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              900  CCAGGACCTGG         
RsaI           36  GTAC                 GT^AC
              552  GTAC                
Sau96I         87  GGCCC                G^G[ACGT]CC
              903  GGACC               
SfaNI         426  GCATC                GCATC(5/9)
              503  GCATC               
SfcI          507  CTATAG               C^T[GA][CT]AG
              873  CTACAG              
TseI          464  GCAGC                G^C[AT]GC
              617  GCTGC               
Van91I        337  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              900  CCAGGACCTGG         
XmnI           75  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              631  GAAATGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       BfaI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BssKI       BstNI       BstUI       
Cac8I       CfoI        DpnII       EcoRII      FauI        
Fnu4HI      HaeIII      HhaI        HinP1I      ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MvaI        MvnI        MwoI        NdeII       Sau3AI      
ScrFI       TaqI        Tsp45I      TspRI       TthHB8I     
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