Restriction map for
pFN21AB6475
(Circular) (Six-base) MAPSORT of: pFN21AB6475.seq Check: 8955 from: 1 to: 5683
pFN21AB6475 5683 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2952 2952
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2858 3746 5130 5398 1632
Size: 1226 888 1384 268 1917
Fragments arranged by size:
1917 1384 1226 888 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2302 4582 5559 1203
Size: 1099 2280 977 1327
Fragments arranged by size:
2280 1327 1099 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 2600 1166
Size: 1434 4249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2348 2911 3426 4998 2348
Size: 563 515 1572 3033
Fragments arranged by size:
3033 1572 563 515
ApaLI G'TGCA_C
Cuts at: 2251 3740 4896 2251
Size: 1489 1156 3038
Fragments arranged by size:
3038 1489 1156
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 1870
Size: 73 1065 4545
Fragments arranged by size:
4545 1065 73
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2202 3004 4459 618
Size: 325 217 627 282 133 802 1455 1842
Fragments arranged by size:
1842 1455 802 627 325 282 217 133
BanII G_rGCy'C
Cuts at: 1391 3002 1391
Size: 1611 4072
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 5683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2733 928
Size: 971 834 3878
Fragments arranged by size:
3878 971 834
BbvCI CC'TCA_GC
Cuts at: 2198 2198
Size: 5683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2680 1548
Size: 1132 4551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2255 2358 2887 3744 4900 1790
Size: 465 103 529 857 1156 2573
Fragments arranged by size:
2573 1156 857 529 465 103
BmgBI CAC'GTC
Cuts at: 1318 2704 1318
Size: 1386 4297
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2370 2564 2632 4343 1239
Size: 989 142 194 68 1711 2579
Fragments arranged by size:
2579 1711 989 194 142 68
Bpu10I CC'TnA_GC
Cuts at: 2198 2198
Size: 5683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 4346 882
Size: 617 705 2142 2219
Fragments arranged by size:
2219 2142 705 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 2679 5677 2679
Size: 2998 2685
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 3490 3872 4507
Size: 53 83 186 936 537 1420 382 635
Cuts at: 4507 275
Size: 1451
Fragments arranged by size:
1451 1420 936 635 537 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5458
Fragments arranged by size:
5458 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 1186
Size: 985 4698
BseYI C'CCAG_C
Cuts at: 2857 2951 4886 5382 2857
Size: 94 1935 496 3158
Fragments arranged by size:
3158 1935 496 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 1163
Size: 573 429 4681
Fragments arranged by size:
4681 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 2255 2566 3744 3829 4900 2223
Size: 32 311 1178 85 1071 3006
Fragments arranged by size:
3006 1178 1071 311 85 32
BsmI GAATG_Cn'
Cuts at: 2051 3194 3287 2051
Size: 1143 93 4447
Fragments arranged by size:
4447 1143 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2255 2358 2566 2887 3002 3744
Size: 399 433 32 103 208 321 115 742
Cuts at: 3744 3829 4900 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 321 208 115
103 85 32
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2952 3038 844
Size: 2108 86 3489
Fragments arranged by size:
3489 2108 86
BsrBI CCG'CTC
Cuts at: 1394 1823 2170 3570 1394
Size: 429 347 1400 3507
Fragments arranged by size:
3507 1400 429 347
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 4327 4514 1700
Size: 110 340 2177 187 2869
Fragments arranged by size:
2869 2177 340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 2461 1184
Size: 1277 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 2329 2602 2329
Size: 273 5410
BstYI r'GATC_y
Cuts at: 1972 2605 3013 3380 3765 3782 5234 5320 5332
Size: 633 408 367 385 17 1452 86 12
Cuts at: 5332 5678 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 633 408 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2601 513
Size: 552 939 597 3595
Fragments arranged by size:
3595 939 597 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2417 487
Size: 683 56 1191 3753
Fragments arranged by size:
3753 1191 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 2811 1554
Size: 1257 4426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DraIII CAC_nnn'GTG
Cuts at: 2553 2553
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648
Size: 54 1425 50 275 18 1300 889 1629
Cuts at: 5648 8
Size: 43
Fragments arranged by size:
1629 1425 1300 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3613 1353
Size: 2260 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 4270 4644 4790 387
Size: 1064 138 480 2201 374 146 1280
Fragments arranged by size:
2201 1280 1064 480 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2370 2564 2632 2858 3746 4343
Size: 393 596 142 194 68 226 888 597
Cuts at: 4343 5130 5398 1239
Size: 787 268 1524
Fragments arranged by size:
1524 888 787 597 596 393 268 226
194 142 68
EcoO109I rG'GnC_Cy
Cuts at: 2908 2908
Size: 5683
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
EcoRV GAT'ATC
Cuts at: 2809 2809
Size: 5683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2849 2849
Size: 5683
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 2073 4830 1678
Size: 395 2757 2531
Fragments arranged by size:
2757 2531 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373
Size: 32 135 32 2752 32 42 32 2626
Fragments arranged by size:
2752 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 2787 3282 2787
Size: 495 5188
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2600 3641 4000 4159 518
Size: 648 1293 141 1041 359 159 2042
Fragments arranged by size:
2042 1293 1041 648 359 159 141
MspA1I CmG'CkG
Cuts at: 1606 2472 3099 3776 4924 5169 1606
Size: 866 627 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 677 627 245
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 5683
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NruI TCG'CGA
Cuts at: 2436 2436
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2167 2306 3041 4586 1132
Size: 97 938 139 735 1545 2229
Fragments arranged by size:
2229 1545 938 735 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 2302 4582 2302
Size: 2280 3403
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2348 2911 1108
Size: 1240 563 3880
Fragments arranged by size:
3880 1240 563
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2908 2908
Size: 5683
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 3035 830
Size: 2205 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2512 2878 3031 4166 4847 5038 826
Size: 219 1467 366 153 1135 681 191 1471
Fragments arranged by size:
1471 1467 1135 681 366 219 191 153
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 5683
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 2167 3041 1132
Size: 1035 874 3774
Fragments arranged by size:
3774 1035 874
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2004 3122 513
Size: 552 939 1118 3074
Fragments arranged by size:
3074 1118 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2910 3710 3727 3880 4065 4510 325
Size: 1255 1330 800 17 153 185 445 1498
Fragments arranged by size:
1498 1330 1255 800 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 5643 5675 2006
Size: 32 3605 32 2014
Fragments arranged by size:
3605 2014 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2185
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 2640 3812 2084
Size: 556 1172 3955
Fragments arranged by size:
3955 1172 556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI
NaeI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI