Restriction map for pFN21AB6475
 (Linear) (Six-base) MAPSORT of: KIBB6475.seq  Check: 7798  from: 1  to: 978



KIBB6475.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     947     978 
   Size:        947      31
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     853     978 
   Size:        853     125
 
AflIII A'CryG_T

Cuts at:      0     297     978 
   Size:        297     681
 
AleI CACnn'nnGTG

Cuts at:      0     595     978 
   Size:        595     383
 
AlwNI CAG_nnn'CTG

Cuts at:      0     343     906     978 
   Size:        343     563      72

  Fragments arranged by size: 

                 563     343      72
 
ApaLI G'TGCA_C

Cuts at:      0     246     978 
   Size:        246     732
 
BanI G'GyrC_C

Cuts at:      0      64     197     978 
   Size:         64     133     781

  Fragments arranged by size: 

                 781     133      64
 
BbeI G_GCGC'C

Cuts at:      0      68     978 
   Size:         68     910
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     728     978 
   Size:        728     250
 
BbvCI CC'TCA_GC

Cuts at:      0     193     978 
   Size:        193     785
 
BclI T'GATC_A

Cuts at:      0     675     978 
   Size:        675     303
 
Bme1580I G_kGCm'C

Cuts at:      0     250     353     882     978 
   Size:        250     103     529      96

  Fragments arranged by size: 

                 529     250     103      96
 
BmgBI CAC'GTC

Cuts at:      0     699     978 
   Size:        699     279
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     365     559     627     978 
   Size:        223     142     194      68     351

  Fragments arranged by size: 

                 351     223     194     142      68
 
Bpu10I CC'TnA_GC

Cuts at:      0     193     978 
   Size:        193     785
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     199     978 
   Size:        199     779
 
BsaBI GATnn'nnATC

Cuts at:      0     674     978 
   Size:        674     304
 
BsaHI Gr'CG_yC

Cuts at:      0      65     978 
   Size:         65     913
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     166     978 
   Size:        166     812
 
BseYI C'CCAG_C

Cuts at:      0     852     946     978 
   Size:        852      94      32

  Fragments arranged by size: 

                 852      94      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     978 
   Size:        160     818
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     250     561     978 
   Size:        218      32     311     417

  Fragments arranged by size: 

                 417     311     218      32
 
BsmI GAATG_Cn'

Cuts at:      0      46     978 
   Size:         46     932
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     250     353     561     882     978 
   Size:        218      32     103     208     321      96

  Fragments arranged by size: 

                 321     218     208     103      96      32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     947     978 
   Size:        947      31
 
BsrBI CCG'CTC

Cuts at:      0     165     978 
   Size:        165     813
 
BsrFI r'CCGG_y

Cuts at:      0     145     978 
   Size:        145     833
 
BstEII G'GTnAC_C

Cuts at:      0     456     978 
   Size:        456     522
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     324     597     978 
   Size:        324     273     381

  Fragments arranged by size: 

                 381     324     273
 
BstYI r'GATC_y

Cuts at:      0     600     978 
   Size:        600     378
 
BtgI C'CryG_G

Cuts at:      0     596     978 
   Size:        596     382
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     412     978 
   Size:        412     566
 
ClaI AT'CG_AT

Cuts at:      0     806     978 
   Size:        806     172
 
DraIII CAC_nnn'GTG

Cuts at:      0     548     978 
   Size:        548     430
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      64     978 
   Size:         64     914
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     365     559     627     853     978 
   Size:        223     142     194      68     226     125

  Fragments arranged by size: 

                 226     223     194     142     125      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     903     978 
   Size:        903      75
 
EcoRV GAT'ATC

Cuts at:      0     804     978 
   Size:        804     174
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     844     978 
   Size:        844     134
 
HaeII r_GCGC'y

Cuts at:      0      68     978 
   Size:         68     910
 
KasI G'GCGC_C

Cuts at:      0      64     978 
   Size:         64     914
 
MfeI C'AATT_G

Cuts at:      0     782     978 
   Size:        782     196
 
MslI CAynn'nnrTG

Cuts at:      0     454     595     978 
   Size:        454     141     383

  Fragments arranged by size: 

                 454     383     141
 
MspA1I CmG'CkG

Cuts at:      0     467     978 
   Size:        467     511
 
NarI GG'CG_CC

Cuts at:      0      65     978 
   Size:         65     913
 
NruI TCG'CGA

Cuts at:      0     431     978 
   Size:        431     547
 
NspI r_CATG'y

Cuts at:      0     162     301     978 
   Size:        162     139     677

  Fragments arranged by size: 

                 677     162     139
 
PciI A'CATG_T

Cuts at:      0     297     978 
   Size:        297     681
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     343     906     978 
   Size:        343     563      72

  Fragments arranged by size: 

                 563     343      72
 
PfoI T'CCnGG_A

Cuts at:      0     899     978 
   Size:        899      79
 
PpuMI rG'GwC_Cy

Cuts at:      0     903     978 
   Size:        903      75
 
SfcI C'TryA_G

Cuts at:      0     507     873     978 
   Size:        507     366     105

  Fragments arranged by size: 

                 507     366     105
 
SfoI GGC'GCC

Cuts at:      0      66     978 
   Size:         66     912
 
SphI G_CATG'C

Cuts at:      0     162     978 
   Size:        162     816
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     905     978 
   Size:        905      73
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       1      33     978 
   Size:          1      32     945

  Fragments arranged by size: 

                 945      32       1
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     180     978 
   Size:        180     798
 
XmnI GAAnn'nnTTC

Cuts at:      0      79     635     978 
   Size:         79     556     343

  Fragments arranged by size: 

                 556     343      79
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI    ApaLI     BanI     BbeI
     BbsI    BbvCI     BclI Bme1580I    BmgBI     BpmI   Bpu10I     BsaI
    BsaBI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BsrFI   BstEII    BstXI    BstYI     BtgI    BtgZI
     ClaI   DraIII     EciI  Eco57MI EcoO109I    EcoRV     FalI    HaeII
     KasI     MfeI     MslI   MspA1I     NarI     NruI     NspI     PciI
    PflMI     PfoI    PpuMI     SfcI     SfoI     SphI    TaqII     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaWI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     BtsI    CspCI
     DraI     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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