Restriction map for
pFN21AB6449
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbeI BbsI BcgI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 557 GTCTAC GT^[AC][GT]AC
AfaI 123 GTAC GT^AC
ApaI 42 GGGCCC GGGCC^C
Asp700I 823 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 412 GAGCTC G[AT]GC[AT]^C
AspI 489 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 160 CTCGAG C^[CT]CG[GA]G
AvaII 675 GGACC G^G[AT]CC
BanI 174 GGTGCC G^G[CT][GA]CC
BbrPI 552 CACGTG CAC^GTG
BbvCI 379 GCTGAGG CCTCAGC(-5/-2)
BciVI 791 GGATAC GTATCC(6/5)
BclI 356 TGATCA T^GATCA
BcnI 788 CCCGG CC^[GC]GG
BfaI 820 CTAG C^TAG
BsaAI 552 CACGTG [CT]AC^GT[GA]
BsaI 296 GAGACC GGTCTC(1/5)
BsiHKAI 412 GAGCTC G[AT]GC[AT]^C
BsmFI 221 GGGAC GGGAC(10/14)
BsoBI 160 CTCGAG C^[CT]CG[GA]G
BsrBI 67 GAGCGG CCGCTC(-3/-3)
BsrI 75 ACTGG ACTGG(1/-1)
BstAPI 168 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 152 AGATCC [GA]^GATC[CT]
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
FbaI 356 TGATCA T^GATCA
HaeII 17 AGCGCC [GA]GCGC^[CT]
HgaI 939 GCGTC GACGC(5/10)
HincII 727 GTCAAC GT[CT]^[GA]AC
HindII 727 GTCAAC GT[CT]^[GA]AC
MaeI 820 CTAG C^TAG
MflI 152 AGATCC [GA]^GATC[CT]
MslI 270 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 788 CCCGG CC^[GC]GG
PaeR7I 160 CTCGAG C^TCGAG
PflFI 489 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 552 CACGTG CAC^GTG
PmlI 552 CACGTG CAC^GTG
RsaI 123 GTAC GT^AC
SacI 412 GAGCTC GAGCT^C
SexAI 692 ACCTGGT A^CC[AT]GGT
SmlI 160 CTCGAG C^T[CT][GA]AG
Tsp45I 699 GTCAC ^GT[GC]AC
Tsp509I 375 AATT ^AATT
Tth111I 489 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 160 CTCGAG C^TCGAG
XhoII 152 AGATCC [GA]^GATC[CT]
XmnI 823 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 551 ACACGT A^C[GA][CT]GT
553 ACGTGT
AhdI 691 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACACAGAGTC
AspEI 691 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACACAGAGTC
BseRI 118 GAGGAG GAGGAG(10/8)
856 GAGGAG
BsgI 232 CTGCAC GTGCAG(16/14)
498 CTGCAC
BsiEI 11 CGGCCG CG[GA][CT]^CG
81 CGACCG
BsmAI 296 GAGAC GTCTC(1/5)
419 GAGAC
BspMI 326 ACCTGC ACCTGC(4/8)
677 ACCTGC
CfoI 18 GCGC GCG^C
180 GCGC
DraII 41 AGGGCCC [GA]G^G[ACGT]CC[CT]
42 GGGCCCC
Eam1105I 691 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACACAGAGTC
EcoO109I 41 AGGGCCC [GA]G^G[ACGT]CC[CT]
42 GGGCCCC
FauI 47 CCCGC CCCGC(4/6)
507 CCCGC
HapII 651 CCGG C^CGG
789 CCGG
HhaI 18 GCGC GCG^C
180 GCGC
HinP1I 18 GCGC G^CGC
180 GCGC
HpaII 651 CCGG C^CGG
789 CCGG
MaeII 553 ACGT A^CGT
946 ACGT
MaeIII 699 GTCAC ^GT[ACGT]AC
736 GTTAC
MboII 60 GAAGA GAAGA(8/7)
253 GAAGA
MspI 651 CCGG C^CGG
789 CCGG
PstI 145 CTGCAG CTGCA^G
328 CTGCAG
PvuII 802 CAGCTG CAG^CTG
811 CAGCTG
SfaNI 834 GATGC GCATC(5/9)
590 GCATC
SfcI 145 CTGCAG C^T[GA][CT]AG
328 CTGCAG
TaqI 161 TCGA T^CGA
426 TCGA
TspRI 263 CACTG CA[GC]TG[ACGT][ACGT]^
671 CAGTG
TthHB8I 161 TCGA T^CGA
426 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
BalI BanII BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MboI MluNI MnlI MscI
MspA1I MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI StyI TseI