(Circular) (Six-base) MAPSORT of: pFN21AB6449.seq Check: 3623 from: 1 to: 5656 pFN21AB6449 5656 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2563 2999 1937 Size: 626 436 4594 Fragments arranged by size: 4594 626 436 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2552 2819 3719 5103 5371 1632 Size: 452 468 267 900 1384 268 1917 Fragments arranged by size: 1917 1384 900 468 452 268 267 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2556 2558 4555 5532 1203 Size: 1353 2 1997 977 1327 Fragments arranged by size: 1997 1353 1327 977 2 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 2701 2878 4385 2701 Size: 177 1507 3972 Fragments arranged by size: 3972 1507 177 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2809 2842 2947 3399 4971 2809 Size: 33 105 452 1572 3494 Fragments arranged by size: 3494 1572 452 105 33 ApaI G_GGCC'C Cuts at: 2051 2051 Size: 5656 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2165 2981 1870 Size: 73 222 816 4545 Fragments arranged by size: 4545 816 222 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2977 4432 618 Size: 325 217 627 392 798 1455 1842 Fragments arranged by size: 1842 1455 798 627 392 325 217 BanII G_rGCy'C Cuts at: 1391 2051 2199 2421 2646 2975 1391 Size: 660 148 222 225 329 4072 Fragments arranged by size: 4072 660 329 225 222 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4685 BbvCI CC'TCA_GC Cuts at: 2385 2385 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2790 3548 4758 2790 Size: 758 1210 3688 Fragments arranged by size: 3688 1210 758 BclI T'GATC_A Cuts at: 1548 2361 1548 Size: 813 4843 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2051 3717 4873 1790 Size: 261 1666 1156 2573 Fragments arranged by size: 2573 1666 1156 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4316 1239 Size: 3077 2579 Bpu10I CC'TnA_GC Cuts at: 2385 2415 2637 2385 Size: 30 222 5404 Fragments arranged by size: 5404 222 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2295 4319 882 Size: 617 796 2024 2219 Fragments arranged by size: 2219 2024 796 617 BsaAI yAC'GTr Cuts at: 493 1204 2559 3447 493 Size: 711 1355 888 2702 Fragments arranged by size: 2702 1355 888 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 1178 Size: 30 165 30 1104 30 4297 Fragments arranged by size: 4297 1104 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2876 1186 Size: 952 738 3966 Fragments arranged by size: 3966 952 738 BseYI C'CCAG_C Cuts at: 2809 4859 5355 2809 Size: 2050 496 3110 Fragments arranged by size: 3110 2050 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2222 2488 1163 Size: 573 486 266 4331 Fragments arranged by size: 4331 573 486 266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2089 3106 3867 4895 1540 Size: 275 186 18 70 1017 761 1028 2301 Fragments arranged by size: 2301 1028 1017 761 275 186 70 18 BsiHKAI G_wGCw'C Cuts at: 2421 3717 3802 4873 2421 Size: 1296 85 1071 3204 Fragments arranged by size: 3204 1296 1071 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2199 2421 2646 2975 3717 3802 Size: 399 261 148 222 225 329 742 85 Cuts at: 3802 4873 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 329 261 225 222 148 85 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 2691 3011 844 Size: 1496 351 320 3489 Fragments arranged by size: 3489 1496 351 320 BsrBI CCG'CTC Cuts at: 1394 1823 2074 3543 1394 Size: 429 251 1469 3507 Fragments arranged by size: 3507 1469 429 251 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 2179 794 Size: 1385 4271 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2157 2986 3353 3738 3755 5207 5293 5305 Size: 185 829 367 385 17 1452 86 12 Cuts at: 5305 5651 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 829 385 367 346 185 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 2274 2581 513 Size: 552 975 234 307 3588 Fragments arranged by size: 3588 975 552 307 234 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 2682 3172 3966 3986 2682 Size: 490 794 20 4352 Fragments arranged by size: 4352 794 490 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2334 2369 529 Size: 35 1770 35 3816 Fragments arranged by size: 3816 1770 35 35 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2244 2475 Size: 54 1425 50 275 18 186 228 231 Cuts at: 2475 2551 2653 3103 3992 5621 8 Size: 76 102 450 889 1629 43 Fragments arranged by size: 1629 1425 889 450 275 231 228 186 102 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 3103 1537 Size: 275 204 1087 4090 Fragments arranged by size: 4090 1087 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 3586 1353 Size: 2233 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2150 2276 4243 4617 4763 387 Size: 1064 138 561 126 1967 374 146 1280 Fragments arranged by size: 1967 1280 1064 561 374 146 138 126 EcoICRI GAG'CTC Cuts at: 2419 2419 Size: 5656 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2552 2819 3719 4316 5103 5371 Size: 393 452 468 267 900 597 787 268 Cuts at: 5371 1239 Size: 1524 Fragments arranged by size: 1524 900 787 597 468 452 393 268 267 EcoO109I rG'GnC_Cy Cuts at: 2047 2048 2047 Size: 1 5655 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 2026 4803 1678 Size: 348 2777 2531 Fragments arranged by size: 2777 2531 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2687 2719 2864 Size: 32 135 32 641 32 442 32 145 Cuts at: 2864 2896 4297 4329 4371 4403 1373 Size: 32 1401 32 42 32 2626 Fragments arranged by size: 2626 1401 641 442 145 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2734 3000 3246 669 Size: 988 281 796 266 246 3079 Fragments arranged by size: 3079 988 796 281 266 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2246 2477 2553 10 Size: 54 1425 343 414 231 76 3113 Fragments arranged by size: 3113 1425 414 343 231 76 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 3614 3973 4132 518 Size: 648 1113 1335 359 159 2042 Fragments arranged by size: 2042 1335 1113 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 2809 2818 3072 3749 4897 5142 1606 Size: 494 709 9 254 677 1148 245 2120 Fragments arranged by size: 2120 1148 709 677 494 254 245 9 NcoI C'CATG_G Cuts at: 513 1065 2040 2274 2581 513 Size: 552 975 234 307 3588 Fragments arranged by size: 3588 975 552 307 234 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 3014 4559 1132 Size: 97 1785 1545 2229 Fragments arranged by size: 2229 1785 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PmlI CAC'GTG Cuts at: 2559 2559 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2870 2902 3725 3757 5238 5270 2870 Size: 32 823 32 1481 32 3256 Fragments arranged by size: 3256 1481 823 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PspOMI G'GGCC_C Cuts at: 2047 2047 Size: 5656 PspXI vC'TCGA_Gb Cuts at: 2165 2165 Size: 5656 PstI C_TGCA'G Cuts at: 830 2154 2337 3008 830 Size: 1324 183 671 3478 Fragments arranged by size: 3478 1324 671 183 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2809 2818 2809 Size: 9 5647 SacI G_AGCT'C Cuts at: 2421 2421 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SexAI A'CCwGG_T Cuts at: 2697 2697 Size: 5656 SfcI C'TryA_G Cuts at: 826 1045 2150 2333 3004 4139 4820 5011 826 Size: 219 1105 183 671 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 681 671 219 191 183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 3760 4661 4923 820 Size: 197 926 222 1595 901 262 1553 Fragments arranged by size: 1595 1553 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2040 2274 2581 2616 3095 513 Size: 552 975 234 307 35 479 3074 Fragments arranged by size: 3074 975 552 479 307 234 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2609 3683 3700 3853 4038 4483 325 Size: 1255 1029 1074 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1074 1029 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2507 2539 5616 5648 2507 Size: 32 3077 32 2515 Fragments arranged by size: 3077 2515 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2497 1316 Size: 1181 4475 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 2165 1943 Size: 222 5434 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2832 3785 2832 Size: 953 4703 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI