(Circular) (Six-base) MAPSORT of: pFN21AB6442.seq Check: 2256 from: 1 to: 5743 pFN21AB6442 5743 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2439 2439 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2870 3553 278 Size: 53 83 186 936 1334 683 2468 Fragments arranged by size: 2468 1334 936 683 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 2613 3064 2613 Size: 451 5292 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2092 2147 2318 3806 5190 5458 1632 Size: 460 55 171 1488 1384 268 1917 Fragments arranged by size: 1917 1488 1384 460 268 171 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2066 2098 2066 Size: 32 5711 AlwNI CAG_nnn'CTG Cuts at: 2643 3038 3486 5058 2643 Size: 395 448 1572 3328 Fragments arranged by size: 3328 1572 448 395 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2891 3068 1870 Size: 73 948 177 4545 Fragments arranged by size: 4545 948 177 73 AvrII C'CTAG_G Cuts at: 2113 2113 Size: 5743 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2613 3064 4519 618 Size: 325 217 627 826 451 1455 1842 Fragments arranged by size: 1842 1455 826 627 451 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2378 2406 2733 3062 1391 Size: 673 314 28 327 329 4072 Fragments arranged by size: 4072 673 329 327 314 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 2471 928 Size: 971 316 256 4200 Fragments arranged by size: 4200 971 316 256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3035 4354 136 Size: 107 122 71 631 605 1363 1319 1525 Fragments arranged by size: 1525 1363 1319 631 605 122 107 71 BglII A'GATC_T Cuts at: 2244 5738 2244 Size: 3494 2249 BlpI GC'TnA_GC Cuts at: 2768 3160 2768 Size: 392 5351 Bme1580I G_kGCm'C Cuts at: 1790 2187 3804 4960 1790 Size: 397 1617 1156 2573 Fragments arranged by size: 2573 1617 1156 397 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 2948 2984 4403 1239 Size: 1163 546 36 1419 2579 Fragments arranged by size: 2579 1419 1163 546 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2390 3868 4733 5031 2390 Size: 1478 865 298 3102 Fragments arranged by size: 3102 1478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2939 4406 882 Size: 617 1440 1467 2219 Fragments arranged by size: 2219 1467 1440 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 2628 2867 3550 Size: 53 83 186 936 723 372 239 683 Cuts at: 3550 3932 4567 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 723 683 635 382 372 239 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 2552 2582 1178 Size: 30 165 30 663 30 456 30 4339 Fragments arranged by size: 4339 663 456 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2161 2741 2804 1186 Size: 975 580 63 4125 Fragments arranged by size: 4125 975 580 63 BseYI C'CCAG_C Cuts at: 2237 2266 2980 4946 5442 2237 Size: 29 714 1966 496 2538 Fragments arranged by size: 2538 1966 714 496 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2733 3804 3889 4960 2733 Size: 1071 85 1071 3516 Fragments arranged by size: 3516 1071 1071 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2187 2378 2406 2733 3062 3804 Size: 399 274 123 191 28 327 329 742 Cuts at: 3804 3889 4960 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 329 327 274 191 123 85 28 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 2070 2457 2787 3632 5362 2070 Size: 387 330 845 1730 2451 Fragments arranged by size: 2451 1730 845 387 330 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2439 3098 844 Size: 1595 659 3489 Fragments arranged by size: 3489 1595 659 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 2833 1184 Size: 1649 4094 BstYI r'GATC_y Cuts at: 1972 2244 3073 3440 3825 3842 5294 5380 5392 Size: 272 829 367 385 17 1452 86 12 Cuts at: 5392 5738 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 829 385 367 346 272 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2660 2796 513 Size: 552 939 656 136 3460 Fragments arranged by size: 3460 939 656 552 136 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2166 487 Size: 683 56 940 4064 Fragments arranged by size: 4064 940 683 56 BtsI GCAGTG_nn' Cuts at: 3259 4053 4073 3259 Size: 794 20 4929 Fragments arranged by size: 4929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3190 4079 5708 Size: 54 1425 50 275 18 1360 889 1629 Cuts at: 5708 8 Size: 43 Fragments arranged by size: 1629 1425 1360 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2345 2831 2927 3673 1353 Size: 977 15 486 96 746 3423 Fragments arranged by size: 3423 977 746 486 96 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2731 2731 Size: 5743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2092 2147 2318 2402 2948 2984 3806 Size: 393 460 55 171 84 546 36 822 Cuts at: 3806 4403 5190 5458 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 822 787 597 546 460 393 268 171 84 55 36 EcoO109I rG'GnC_Cy Cuts at: 3028 3028 Size: 5743 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2012 4890 1678 Size: 334 2878 2531 Fragments arranged by size: 2878 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2533 2552 2565 2584 2867 Size: 32 135 32 961 19 13 19 283 Cuts at: 2867 2899 4384 4416 4458 4490 1373 Size: 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 961 283 135 42 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 2617 3068 2617 Size: 451 5292 MfeI C'AATT_G Cuts at: 2844 3342 2844 Size: 498 5245 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2910 4857 5041 1305 Size: 1605 1947 184 2007 Fragments arranged by size: 2007 1947 1605 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 3701 4060 4219 518 Size: 648 1308 1227 359 159 2042 Fragments arranged by size: 2042 1308 1227 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2640 3159 3836 4984 5229 1606 Size: 1034 519 677 1148 245 2120 Fragments arranged by size: 2120 1148 1034 677 519 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2796 513 Size: 552 939 792 3460 Fragments arranged by size: 3460 939 792 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2479 3101 4646 1132 Size: 97 1250 622 1545 2229 Fragments arranged by size: 2229 1545 1250 622 97 PasI CC'CwG_GG Cuts at: 1348 2392 1348 Size: 1044 4699 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 3038 1108 Size: 1930 3813 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2066 2098 3812 3844 5325 5357 2066 Size: 32 1714 32 1481 32 2452 Fragments arranged by size: 2452 1714 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PstI C_TGCA'G Cuts at: 830 2484 2756 3095 830 Size: 1654 272 339 3478 Fragments arranged by size: 3478 1654 339 272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2640 2640 Size: 5743 SacI G_AGCT'C Cuts at: 2733 2733 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SapI GCTCTTCn'nnn_ Cuts at: 2831 2927 2831 Size: 96 5647 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 2423 3991 5703 2423 Size: 1568 1712 2463 Fragments arranged by size: 2463 1712 1568 SexAI A'CCwGG_T Cuts at: 2616 2994 2616 Size: 378 5365 SfcI C'TryA_G Cuts at: 826 1045 2480 2704 2752 2776 2861 3091 4226 Size: 219 1435 224 48 24 85 230 1135 Cuts at: 4226 4907 5098 826 Size: 681 191 1471 Fragments arranged by size: 1471 1435 1135 681 230 224 219 191 85 48 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 3847 4748 5010 820 Size: 197 926 462 1442 901 262 1553 Fragments arranged by size: 1553 1442 926 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 2957 1517 Size: 1440 4303 StyI C'CwwG_G Cuts at: 513 1065 2004 2056 2113 2398 2796 3182 513 Size: 552 939 52 57 285 398 386 3074 Fragments arranged by size: 3074 939 552 398 386 285 57 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2731 2789 3770 3787 3940 4125 4570 Size: 1255 1151 58 981 17 153 185 445 Cuts at: 4570 325 Size: 1498 Fragments arranged by size: 1498 1255 1151 981 445 185 153 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 3989 5701 Size: 274 80 33 51 1443 444 1568 1712 Cuts at: 5701 96 Size: 138 Fragments arranged by size: 1712 1568 1443 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 2627 1316 Size: 1311 4432 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 2078 3872 2078 Size: 1794 3949 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2868 3551 276 Size: 53 83 186 936 1334 683 2468 Fragments arranged by size: 2468 1334 936 683 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI