Restriction map for
pFN21AB6431
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII AccI AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BalI
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BpuAI BsaAI
BsaBI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
MaeII MamI MfeI MflI MluI
MluNI MscI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 386 GGTACC G^GTACC
AccIII 116 TCCGGA T^CCGGA
AcyI 445 GGCGCC G[GA]^CG[CT]C
ApaI 652 GGGCCC GGGCC^C
Asp718I 386 GGTACC G^GTACC
AvrII 811 CCTAGG C^CTAGG
BbeI 445 GGCGCC GGCGC^C
BbvCI 744 GCTGAGG CCTCAGC(-5/-2)
BfaI 812 CTAG C^TAG
BglI 616 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 811 CCTAGG C^CTAGG
BlpI 235 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 235 GCTGAGC GC^T[ACGT]AGC
BsaHI 445 GGCGCC G[GA]^CG[CT]C
BsaWI 116 TCCGGA [AT]^CCGG[AT]
BseAI 116 TCCGGA T^CCGGA
BsiEI 920 CGGCCG CG[GA][CT]^CG
BspEI 116 TCCGGA T^CCGGA
BspMI 383 GCAGGT ACCTGC(4/8)
BsrDI 705 CATTGC GCAATG(2/0)
BssSI 826 CACGAG CACGAG(-5/-1)
BstAPI 453 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 235 GCTGAGC GC^T[ACGT]AGC
DpnII 831 GATC ^GATC
EagI 920 CGGCCG C^GGCCG
EarI 122 GAAGAG CTCTTC(1/4)
EclXI 920 CGGCCG C^GGCCG
Eco52I 920 CGGCCG C^GGCCG
EcoT14I 811 CCTAGG C^C[AT][AT]GG
HgaI 168 GCGTC GACGC(5/10)
Hin1I 445 GGCGCC G[GA]^CG[CT]C
HincII 289 GTCAAC GT[CT]^[GA]AC
HindII 289 GTCAAC GT[CT]^[GA]AC
KasI 445 GGCGCC G^GCGCC
KpnI 386 GGTACC GGTAC^C
Ksp632I 122 GAAGAG CTCTTC(1/4)
MaeI 812 CTAG C^TAG
MboI 831 GATC ^GATC
MboII 122 GAAGA GAAGA(8/7)
MroI 116 TCCGGA T^CCGGA
NarI 445 GGCGCC GG^CGCC
NdeII 831 GATC ^GATC
NspI 720 GCATGT [GA]CATG^[CT]
PpuMI 69 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 283 CAGCTG CAG^CTG
Sau3AI 831 GATC ^GATC
SfoI 445 GGCGCC GGC^GCC
StyI 811 CCTAGG C^C[AT][AT]GG
TfiI 731 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 379 GTAC GT^AC
387 GTAC
Alw44I 724 GTGCAC G^TGCAC
877 GTGCAC
ApaLI 724 GTGCAC G^TGCAC
877 GTGCAC
AvaI 73 CCCGGG C^[CT]CG[GA]G
106 CCCGGG
BsaI 781 GAGACC GGTCTC(1/5)
931 GGTCTC
BsoBI 73 CCCGGG C^[CT]CG[GA]G
106 CCCGGG
DraII 69 GGGACCC [GA]G^G[ACGT]CC[CT]
470 GGGGCCT
DsaI 85 CCGCGG C^C[GA][CT]GG
172 CCGCGG
EcoO109I 69 GGGACCC [GA]G^G[ACGT]CC[CT]
470 GGGGCCT
KspI 85 CCGCGG CCGC^GG
172 CCGCGG
MaeIII 402 GTCAC ^GT[ACGT]AC
436 GTGAC
RsaI 379 GTAC GT^AC
387 GTAC
SacII 85 CCGCGG CCGC^GG
172 CCGCGG
SexAI 389 ACCAGGT A^CC[AT]GGT
431 ACCAGGT
SmaI 73 CCCGGG CCC^GGG
106 CCCGGG
SmlI 33 CTTGAG C^T[CT][GA]AG
244 CTCAAG
Tsp45I 402 GTCAC ^GT[GC]AC
436 GTGAC
XmaCI 73 CCCGGG C^CCGGG
106 CCCGGG
XmaI 73 CCCGGG C^CCGGG
106 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PstI
Sau96I ScrFI SfaNI SfcI TseI
TspRI