(Circular) (Six-base) MAPSORT of: pFN21AB6431.seq Check: 661 from: 1 to: 5641 pFN21AB6431 5641 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2391 2962 2391 Size: 571 5070 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3704 5088 5356 1632 Size: 2072 1384 268 1917 Fragments arranged by size: 2072 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2604 2726 1166 Size: 1438 122 4081 Fragments arranged by size: 4081 1438 122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2194 2401 2464 2624 3384 4956 2194 Size: 207 63 160 760 1572 2879 Fragments arranged by size: 2879 1572 760 207 160 63 ApaI G_GGCC'C Cuts at: 2661 2661 Size: 5641 ApaLI G'TGCA_C Cuts at: 2729 2882 3698 4854 2729 Size: 153 816 1156 3516 Fragments arranged by size: 3516 1156 816 153 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2111 2966 1870 Size: 73 135 33 855 4545 Fragments arranged by size: 4545 855 135 73 33 AvrII C'CTAG_G Cuts at: 2816 2816 Size: 5641 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2375 2408 2375 Size: 33 5608 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2254 2391 2450 2962 4417 Size: 325 217 627 467 137 59 512 1455 Cuts at: 4417 618 Size: 1842 Fragments arranged by size: 1842 1455 627 512 467 325 217 137 59 BanII G_rGCy'C Cuts at: 1391 2112 2216 2247 2271 2661 2960 1391 Size: 721 104 31 24 390 299 4072 Fragments arranged by size: 4072 721 390 299 104 31 24 BbeI G_GCGC'C Cuts at: 2454 2454 Size: 5641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BbvCI CC'TCA_GC Cuts at: 2750 2750 Size: 5641 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2627 4252 136 Size: 107 122 71 631 605 955 1625 1525 Fragments arranged by size: 1625 1525 955 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2241 3058 2241 Size: 817 4824 Bme1580I G_kGCm'C Cuts at: 1790 2374 2661 2699 2733 2886 3702 4858 1790 Size: 584 287 38 34 153 816 1156 2573 Fragments arranged by size: 2573 1156 816 584 287 153 38 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2350 2404 2569 2822 4301 1239 Size: 1111 54 165 253 1479 2579 Fragments arranged by size: 2579 1479 1111 253 165 54 Bpu10I CC'TnA_GC Cuts at: 2750 2750 Size: 5641 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2059 2234 3766 4631 4929 2059 Size: 175 1532 865 298 2771 Fragments arranged by size: 2771 1532 865 298 175 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2780 2942 4304 882 Size: 617 1281 162 1362 2219 Fragments arranged by size: 2219 1362 1281 617 162 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2451 3448 3830 4465 Size: 53 83 186 936 918 997 382 635 Cuts at: 4465 275 Size: 1451 Fragments arranged by size: 1451 997 936 918 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2121 4074 4472 4746 4893 5304 2121 Size: 1953 398 274 147 411 2458 Fragments arranged by size: 2458 1953 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2566 2798 2819 1186 Size: 934 446 232 21 4008 Fragments arranged by size: 4008 934 446 232 21 BseYI C'CCAG_C Cuts at: 2084 2518 2617 4844 5340 2084 Size: 434 99 2227 496 2385 Fragments arranged by size: 2385 2227 496 434 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2928 3091 3852 4880 1540 Size: 275 186 927 163 761 1028 2301 Fragments arranged by size: 2301 1028 927 761 275 186 163 BsiHKAI G_wGCw'C Cuts at: 2355 2733 2886 3702 3787 4858 2355 Size: 378 153 816 85 1071 3138 Fragments arranged by size: 3138 1071 816 378 153 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2216 2247 2271 2355 2374 2661 Size: 399 322 104 31 24 84 19 287 Cuts at: 2661 2699 2733 2886 2960 3702 3787 4858 1391 Size: 38 34 153 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 322 287 153 104 85 84 74 38 34 31 24 19 BspEI T'CCGG_A Cuts at: 2121 5304 2121 Size: 3183 2458 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 2996 844 Size: 1535 617 3489 Fragments arranged by size: 3489 1535 617 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2709 4136 4310 65 Size: 1183 1461 1427 174 1396 Fragments arranged by size: 1461 1427 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4285 4472 1700 Size: 110 2475 187 2869 Fragments arranged by size: 2869 2475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 2831 3701 4713 2831 Size: 870 1012 3759 Fragments arranged by size: 3759 1012 870 BstAPI GCAn_nnn'nTGC Cuts at: 794 2464 794 Size: 1670 3971 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636 Size: 999 367 385 17 1452 86 12 346 Cuts at: 5636 1972 Size: 1977 Fragments arranged by size: 1977 1452 999 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2090 2177 513 Size: 552 1025 87 3977 Fragments arranged by size: 3977 1025 552 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2353 2721 3157 3951 3971 2353 Size: 368 436 794 20 4023 Fragments arranged by size: 4023 794 436 368 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 2860 2925 Size: 54 1425 50 275 18 798 232 65 Cuts at: 2925 3088 3977 5606 8 Size: 163 889 1629 43 Fragments arranged by size: 1629 1425 889 798 275 232 163 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2925 3088 1537 Size: 275 1113 163 4090 Fragments arranged by size: 4090 1113 275 163 EarI CTCTTCn'nnn_ Cuts at: 1353 2122 3571 1353 Size: 769 1449 3423 Fragments arranged by size: 3423 1449 769 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2350 2404 2569 2822 3704 4301 5088 Size: 393 718 54 165 253 882 597 787 Cuts at: 5088 5356 1239 Size: 268 1524 Fragments arranged by size: 1524 882 787 718 597 393 268 253 165 54 EcoO109I rG'GnC_Cy Cuts at: 2075 2476 2075 Size: 401 5240 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 2454 2586 2645 4788 1678 Size: 776 132 59 2143 2531 Fragments arranged by size: 2531 2143 776 132 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2296 2985 3231 669 Size: 988 281 358 689 246 3079 Fragments arranged by size: 3079 988 689 358 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KasI G'GCGC_C Cuts at: 2450 2450 Size: 5641 KpnI G_GTAC'C Cuts at: 2395 2966 2395 Size: 571 5070 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2358 2604 2714 2726 3599 3958 4117 Size: 648 1192 246 110 12 873 359 159 Cuts at: 4117 518 Size: 2042 Fragments arranged by size: 2042 1192 873 648 359 246 159 110 12 MspA1I CmG'CkG Cuts at: 1606 2092 2179 2290 3057 3734 4882 5127 1606 Size: 486 87 111 767 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 677 486 245 111 87 NarI GG'CG_CC Cuts at: 2451 2451 Size: 5641 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5089 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2729 2999 4544 1132 Size: 97 1500 270 1545 2229 Fragments arranged by size: 2229 1545 1500 270 97 PasI CC'CwG_GG Cuts at: 1348 2932 1348 Size: 1584 4057 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2075 2075 Size: 5641 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2657 2657 Size: 5641 PstI C_TGCA'G Cuts at: 830 2140 2502 2595 2993 830 Size: 1310 362 93 398 3478 Fragments arranged by size: 3478 1310 398 362 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2290 2290 Size: 5641 SacII CC_GC'GG Cuts at: 2093 2180 2093 Size: 87 5554 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SanDI GG'GwC_CC Cuts at: 2075 2075 Size: 5641 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SexAI A'CCwGG_T Cuts at: 2394 2436 2394 Size: 42 5599 SfcI C'TryA_G Cuts at: 826 1045 2136 2498 2591 2989 4124 4805 4996 Size: 219 1091 362 93 398 1135 681 191 Cuts at: 4996 826 Size: 1471 Fragments arranged by size: 1471 1135 1091 681 398 362 219 191 93 SfoI GGC'GCC Cuts at: 2452 2452 Size: 5641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2080 2113 2968 1872 Size: 208 33 855 4545 Fragments arranged by size: 4545 855 208 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 2038 2249 3745 4646 4908 820 Size: 197 926 95 211 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 262 211 197 95 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SrfI GCCC'GGGC Cuts at: 2113 2113 Size: 5641 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2816 3080 513 Size: 552 1751 264 3074 Fragments arranged by size: 3074 1751 552 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3887 5599 96 Size: 274 80 33 51 1443 1910 1712 138 Fragments arranged by size: 1910 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2835 2867 5601 5633 2835 Size: 32 2734 32 2843 Fragments arranged by size: 2843 2734 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2078 2111 2966 1870 Size: 208 33 855 4545 Fragments arranged by size: 4545 855 208 33 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SfiI SwaI XcmI